YRC Logo
PROTEIN SEARCH:
Descriptions Names[Advanced Search]

Protein Overview: CEG1

General Information:
Name(s) found: CEG1 / YGL130W [SGD]
Description(s) found:
Found 26 descriptions.
SHOW ALL
  • Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphophatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 459 amino acids

    Gene Ontology:
    Cellular Component: nucleus [IPILink to PubMed article]
    Biological Process: mRNA capping [TASLink to PubMed articleLink to PubMed article]
    positive regulation of transcription from RNA polymerase II promoter [IGILink to PubMed article][IMPLink to PubMed article]
    Molecular Function: mRNA guanylyltransferase activity [TASLink to PubMed articleLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVLAMESRVA PEIPGLIQPG NVTQDLKMMV CKLLNSPKPT KTFPGSQPVS FQHSDVEEKL  60
   61 LAHDYYVCEK TDGLRVLMFI VINPVTGEQG CFMIDRENNY YLVNGFRFPR LPQKKKEELL 120
  121 ETLQDGTLLD GELVIQTNPM TKLQELRYLM FDCLAINGRC LTQSPTSSRL AHLGKEFFKP 180
  181 YFDLRAAYPN RCTTFPFKIS MKHMDFSYQL VKVAKSLDKL PHLSDGLIFT PVKAPYTAGG 240
  241 KDSLLLKWKP EQENTVDFKL ILDIPMVEDP SLPKDDRNRW YYNYDVKPVF SLYVWQGGAD 300
  301 VNSRLKHFDQ PFDRKEFEIL ERTYRKFAEL SVSDEEWQNL KNLEQPLNGR IVECAKNQET 360
  361 GAWEMLRFRD DKLNGNHTSV VQKVLESIND SVSLEDLEEI VGDIKRCWDE RRANMAGGSG 420
  421 RPLPSQSQNA TLSTSKPVHS QPPSNDKEPK YVDEDDWSD

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) CEG1 CET1
    View Details (MIPS) Mewes HW, et al. (2004) CEG1 CET1
    View Details Krogan NJ, et al. (2006) CEG1 CET1 YHR130C
    View Details Gavin AC, et al. (2006) CEG1 CET1
    View Details Gavin AC, et al. (2002) AVO1 BEM2 CEG1 CET1 CLU1 CTR9 HHF1, HHF2 LSM12 NIP1 NOP1 PDB1 PRP43 RPG1 SPT16 SSA3 SSA4 TOP2 VPS1
    View Details Ho Y, et al. (2002) CDC28 CEG1 CKA1 CKA2 CKB1 CKB2 FKH1 FYV8 GCD2 GCD7 GCN3 HHF1, HHF2 HTB1 MBP1 MGM101 MPH1 NET1 NOP1 RRP1 SEC2 SIN3 SUI2 SUI3 UBP12 URE2 YGR017W YMR144W

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run ZDS1 Sample 3 - cpn + atp, from june 2005 McCusker D, et al (2007)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen PRR1 CEG1 1 Unpublished Fields Lab Data
    View Screen HTZ1 CEG1 1 Unpublished Fields Lab Data

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data CEG1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..47]
    [72..198]    		 PSI-BLAST 149.218487 RNA guanylyltransferase (mRNA capping enzyme); RNA guanylyltransferase (mRNA capping enzyme), N-terminal domain
    2 View Details [48..71]
    [199..253]    		 PSI-BLAST 149.218487 RNA guanylyltransferase (mRNA capping enzyme); RNA guanylyltransferase (mRNA capping enzyme), N-terminal domain
    3 View Details [254..338] PSI-BLAST 149.218487 RNA guanylyltransferase (mRNA capping enzyme); RNA guanylyltransferase (mRNA capping enzyme), N-terminal domain
    4 View Details [339..459] ORFEUS 10.46 RNA guanylyltransferase (mRNA capping enzyme); RNA guanylyltransferase (mRNA capping enzyme), N-terminal domain

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • mRNA guanylyltransferase activity
    		•  8.90512192081791 bayes_pls_golite062009
  • RNA guanylyltransferase activity
    		•  8.46124967325993 bayes_pls_golite062009
  • ligase activity, forming phosphoric ester bonds
    		•  2.90848936326491 bayes_pls_golite062009
  • DNA ligase activity
    		•  2.88630907730339 bayes_pls_golite062009
  • nucleic acid binding
    		•  2.49304224824605 bayes_pls_golite062009
  • ligase activity
    		•  2.44151794329612 bayes_pls_golite062009
  • RNA binding
    		•  2.41160981373213 bayes_pls_golite062009
  • DNA binding
    		•  2.3408239279957 bayes_pls_golite062009
  • catalytic activity
    		•  2.15370807854636 bayes_pls_golite062009
  • transcription regulator activity
    		•  2.10110937001387 bayes_pls_golite062009
  • sequence-specific DNA binding
    		•  1.9581702424972 bayes_pls_golite062009
  • binding
    		•  1.82998145778732 bayes_pls_golite062009
  • structure-specific DNA binding
    		•  1.81524862921454 bayes_pls_golite062009
  • guanylyltransferase activity
    		•  1.64088197294498 bayes_pls_golite062009
  • single-stranded DNA binding
    		•  1.62996999055328 bayes_pls_golite062009
    		• 1.5765009871268 bayes_pls_golite062009
  • structural constituent of ribosome
    		•  1.51215866188241 bayes_pls_golite062009
  • protein binding
    		•  1.38006517521813 bayes_pls_golite062009
  • transcription activator activity
    		•  1.24141936405635 bayes_pls_golite062009
  • ribonuclease activity
    		•  1.19236946227657 bayes_pls_golite062009
  • structural molecule activity
    		•  1.16496993393687 bayes_pls_golite062009
  • exoribonuclease activity, producing 5'-phosphomonoesters
    		•  1.15212468568198 bayes_pls_golite062009
  • transcription factor activity
    		•  1.14242112812892 bayes_pls_golite062009
  • exoribonuclease activity
    		•  1.10445667068538 bayes_pls_golite062009
  • DNA ligase (ATP) activity
    		•  1.02324195596817 bayes_pls_golite062009
  • 3'-5'-exoribonuclease activity
    		•  0.83268201349295 bayes_pls_golite062009
  • DNA ligase (NAD+) activity
    		•  0.802565778478831 bayes_pls_golite062009
  • RNA polymerase II transcription factor activity
    		•  0.583085644017496 bayes_pls_golite062009
  • nuclease activity
    		•  0.57381531077345 bayes_pls_golite062009
  • translation regulator activity
    		•  0.515576958825022 bayes_pls_golite062009
  • RNA polymerase activity
    		•  0.510809870824979 bayes_pls_golite062009
  • DNA-directed RNA polymerase activity
    		•  0.510809870824979 bayes_pls_golite062009
  • translation factor activity, nucleic acid binding
    		•  0.468402630523277 bayes_pls_golite062009
  • mRNA binding
    		•  0.4162128308402 bayes_pls_golite062009
  • translation initiation factor activity
    		•  0.2080607201312 bayes_pls_golite062009
  • chromatin binding
    		•  0.19409870825073 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.
    4 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle