Protein Overview: PRP22

General Information:
Name(s) found:	PRP22 / YER013W [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|S13643 PRP22 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6320850\|ref\|NP_010929.1\| DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes; Prp [NCBI NR] gi\|603605\|gb\|AAB64546.1\| Prp22p: pre-mRNA splicing factor RNA helicase [Saccharomyces cerevisiae] [NCBI NR] gi\|4234\|emb\|CAA41530.1\| PRP22 [Saccharomyces cerevisiae] [NCBI NR] gi\|130830\|sp\|P24384.1\|PRP22_YEAST RecName: Full=Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22 [NCBI NR] Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22 OS=Saccharomyces cerevisiae GN=PRP22 PE=1 SV=1 [Swiss-Prot] (P24384) Pre-mRNA splicing factor RNA helicase PRP22 [Swiss-Prot] PRP22 Pre-mRNA splicing factor [mips] DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes [SGD] PRP22, Chr V from 178840-182277 [yeast_orfs2] Chr V from 178840-182277 [yeastorf3.fasta] PRP22 SGDID:S0000815, Chr V from 178840-182277, Verified ORF [yeastflysequest.fasta] Prp22p [Saccharomyces cerevisiae]gi\|4234\|emb\|CAA41530.1\| PRP22 [Saccharomyces cerevisiae]gi\|130830\|sp\|P24384\|PR22_YEAST Pre-mRNA splicing factor RNA helicase PRP22gi\|83433\|pir\|\|S13643 PRP22 protein - yeast (Saccharomyces cerevisiae)gi\|603605\|gb\|AAB645 [nrdb_11152004_Chlamydomonas] PRP22 SGDID:S000000815, Chr V from 178840-182277, Verified ORF, "DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spli [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] PRP22 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Prp22p: pre-mRNA splicing factor RNA helicase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes; Prp22p [Saccharomyces cerevisia [nr-271106-contam.fasta] Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22 [nr-271106-contam.fasta] PRP22 SGDID:S000000815, Chr V from 178840-182277, Verified ORF, "DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PRP22 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] PRP22 SGDID:S000000815, Chr V from 178841-182278, Genome Release 64-1-1, Verified ORF, "DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes" [yeast-030211-contam.fasta] PRP22 SGDID:S000000815, Chr V from 178841-182278, Genome Release 64-2-1, Verified ORF, "DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase; associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes; required for proofreading the exon ligation reaction" [20141208_orf_trans.fasta] YER013W SGDID:S000000815, Chr V from 178841-182278, Genome Release 64-1-1, Verified ORF, "DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1145 amino acids
Gene Ontology:
Cellular Component:	spliceosomal complex [TAS]
Biological Process:	generation of catalytic spliceosome for second transesterification step [IDA]
Molecular Function:	second spliceosomal transesterification activity [IDA] ATP-dependent RNA helicase activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSDISKLIGA IVGSDDPVII EFVLNIINKS GNLQEFIRNI QKLDAGISYE DSIKMYNAFL  60
   61 GKQEEEKVRN KVKSSPLSQK INQVLKDDVN LDDPVVTEFV LSILNKSKSI TEFQEQLNLM 120
  121 QSGLDNETIF KIYQIASPPV MKEEVSVLPS TKIPAKIEAK IEEEVQKIES LDPSPVLHKV 180
  181 YEGKVRNITT FGCFVQIFGT RMKNCDGLVH ISEMSDQRTL DPHDVVRQGQ HIFVEVIKIQ 240
  241 NNGKISLSMK NIDQHSGEIR KRNTESVEDR GRSNDAHTSR NMKNKIKRRA LTSPERWEIR 300
  301 QLIASGAASI DDYPELKDEI PINTSYLTAK RDDGSIVNGN TEKVDSKLEE QQRDETDEID 360
  361 VELNTDDGPK FLKDQQVKGA KKYEMPKITK VPRGFMNRSA INGSNAIRDH REEKLRKKRE 420
  421 IEQQIRKQQS FDDPTKNKKD SRNEIQMLKN QLIVTEWEKN RMNESISYGK RTSLPISAQR 480
  481 QTLPVYAMRS ELIQAVRDNQ FLVIVGETGS GKTTQITQYL DEEGFSNYGM IGCTQPRRVA 540
  541 AVSVAKRVAE EVGCKVGHDV GYTIRFEDVT GPDTRIKYMT DGMLQREALL DPEMSKYSVI 600
  601 MLDEAHERTV ATDVLFALLK KAAIKRPELK VIVTSATLNS AKFSEYFLNC PIINIPGKTF 660
  661 PVEVLYSQTP QMDYIEAALD CVIDIHINEG PGDILVFLTG QEEIDSCCEI LYDRVKTLGD 720
  721 SIGELLILPV YSALPSEIQS KIFEPTPKGS RKVVFATNIA ETSITIDGIY YVVDPGFAKI 780
  781 NIYNARAGIE QLIVSPISQA QANQRKGRAG RTGPGKCYRL YTESAFYNEM LENTVPEIQR 840
  841 QNLSHTILML KAMGINDLLK FDFMDPPPKN LMLNALTELY HLQSLDDEGK LTNLGKEMSL 900
  901 FPMDPTLSRS LLSSVDNQCS DEIVTIISML SVQNVFYRPK DRQLEADSKK AKFHHPYGDH 960
  961 LTLLNVYTRW QQANYSEQYC KTNFLHFRHL KRARDVKSQI SMIFKKIGLK LISCHSDPDL1020
 1021 IRKTFVSGFF MNAAKRDSQV GYKTINGGTE VGIHPSSSLY GKEYEYVMYH SIVLTSREYM1080
 1081 SQVTSIEPQW LLEVAPHFYK AGDAESQSRK KAKIIPLHNK FAKDQNSWRL SSIRQSRERA1140
 1141 LGIKR

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		AAR2 BRR1 BRR2 CBC2 CDC40 CWC15 CWC2 CWC22 CWC23 DHR2 LSM4 MAK5 MUD2 PRP16 PRP18 PRP2 PRP22 PRP24 PRP28 PRP31 PRP38 PRP39 PRP4 PRP40 PRP43 PRP5 PRP6 PRP8 SLU7 SMD1 SMD3 SME1 SNP1 SPP2 SPP381 STO1 YHC1
View Details	Krogan NJ, et al. (2006)		KIP1 PRP22 SOK2
View Details	Gavin AC, et al. (2002)		BUD31 CDC40 CEF1 CLF1 CWC2 CWC22 CWC23 ECM2 HSH155 ISY1 LEA1 PRP19 PRP2 PRP21 PRP22 PRP43 PRP45 PRP46 RSE1 SMD2 SNT309 SNU114 SPP382 STO1 SYF1
View Details	Qiu et al. (2008)		AAR2 BRR1 BRR2 BUD31 CBC2 CDC40 CEF1 CLF1 CUS1 CWC15 CWC2 CWC21 CWC22 CWC23 CWC25 DHR2 DIB1 ECM2 HSH155 IST3 ISY1 LEA1 LSM2 LSM3 LSM4 LSM7 LSM8 LUC7 MAK5 MSL1 MUD1 MUD2 NAM8 NTC20 NTR2 PRP11 PRP16 PRP18 PRP19 PRP2 PRP21 PRP22 PRP24 PRP28 PRP3 PRP31 PRP38 PRP39 PRP4 PRP40 PRP42 PRP43 PRP45 PRP5 PRP6 PRP8 PRP9 RSE1 SLU7 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNP1 SNT309 SNU114 SNU23 SNU56 SNU66 SNU71 SPP2 SPP381 SPP382 STO1 SUB2 SYF1 SYF2 YHC1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	PRP22	Huh WK, et al. (2003)

Searching the YRC Public Image Repository, found results:

Currently showing to of results.

[View Search in the YRC Public Image Repository]

Searching the YRC Public Image Repository, found no results.

Ginzu Version Information

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Gene Ontology predictions

Term	Confidence	Notes
	4.75811129307632	bayes_pls_golite062009
transcription termination factor activity	4.27506184839126	bayes_pls_golite062009
RNA binding	4.05373509885278	bayes_pls_golite062009
structural constituent of ribosome	3.73145378633995	bayes_pls_golite062009
transcription regulator activity	3.24341202600661	bayes_pls_golite062009
structural molecule activity	3.16728233432927	bayes_pls_golite062009
structure-specific DNA binding	1.81524862921454	bayes_pls_golite062009
binding	1.7774714353448	bayes_pls_golite062009
mRNA binding	1.6485756688073	bayes_pls_golite062009
single-stranded DNA binding	1.62996999055328	bayes_pls_golite062009
nucleic acid binding	1.45130233071198	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	1.35850109411274	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	1.35845277283398	bayes_pls_golite062009
ligase activity, forming aminoacyl-tRNA and related compounds	1.35845277283398	bayes_pls_golite062009
ribonuclease activity	1.19236946227657	bayes_pls_golite062009
exoribonuclease activity, producing 5'-phosphomonoesters	1.15212468568198	bayes_pls_golite062009
exoribonuclease activity	1.10445667068538	bayes_pls_golite062009
DNA binding	1.01454217691792	bayes_pls_golite062009
3'-5'-exoribonuclease activity	0.83268201349295	bayes_pls_golite062009
second spliceosomal transesterification activity	0.448378021030889	bayes_pls_golite062009
protein binding	0.423986689445573	bayes_pls_golite062009
ligase activity	0.346041081217685	bayes_pls_golite062009
sequence-specific DNA binding	0.32084431704638	bayes_pls_golite062009
transcription factor activity	0.298729064067577	bayes_pls_golite062009
translation regulator activity	0.22908707234671	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.179682348023561	bayes_pls_golite062009
aspartate-tRNA ligase activity	0.151650064901878	bayes_pls_golite062009
translation initiation factor activity	0.11625379846973	bayes_pls_golite062009
catalytic activity	0.10089766806364	bayes_pls_golite062009
DNA-directed RNA polymerase activity	0.0740938021136293	bayes_pls_golite062009
RNA polymerase activity	0.0740938021136293	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
	4.704140727858	bayes_pls_golite062009
binding	2.44689197844883	bayes_pls_golite062009
RNA binding	2.29590582680214	bayes_pls_golite062009
structural constituent of ribosome	1.57653963868443	bayes_pls_golite062009
RNA helicase activity	1.42791449104435	bayes_pls_golite062009
nucleic acid binding	1.41477043612798	bayes_pls_golite062009
RNA-dependent ATPase activity	1.39335733374328	bayes_pls_golite062009
ATP-dependent RNA helicase activity	1.36724390116598	bayes_pls_golite062009
DNA binding	1.11699929651302	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
transcription regulator activity	0.989226939888218	bayes_pls_golite062009
mRNA binding	0.76699944561918	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	0.652333406433794	bayes_pls_golite062009
helicase activity	0.6191010101321	bayes_pls_golite062009
motor activity	0.61636596887462	bayes_pls_golite062009
structural molecule activity	0.596382945962261	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.461763181659193	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.458831409927227	bayes_pls_golite062009
pyrophosphatase activity	0.455976987338331	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.422944846459286	bayes_pls_golite062009
snRNA binding	0.418985924846052	bayes_pls_golite062009
purine NTP-dependent helicase activity	0.29664013896754	bayes_pls_golite062009
ATP-dependent helicase activity	0.29664013896754	bayes_pls_golite062009
translation release factor activity	0.178286731022211	bayes_pls_golite062009
translation termination factor activity	0.151456842619444	bayes_pls_golite062009
nucleotide binding	0.114848452385726	bayes_pls_golite062009
purine nucleotide binding	0.10713474095071	bayes_pls_golite062009
purine ribonucleotide binding	0.0916519414118148	bayes_pls_golite062009
ribonucleotide binding	0.0915913646217636	bayes_pls_golite062009
protein binding	0.071010918255742	bayes_pls_golite062009
transcription factor activity	0.0434163635422169	bayes_pls_golite062009
cytoskeletal protein binding	0.0272673919333979	bayes_pls_golite062009

No functions predicted.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..122]	MSA	3.158997	View MSA. No confident structure predictions are available.
2	View Details	[123..282]	PSI-BLAST	98.144602	S1 RNA-binding domain of polyribonucleotide phosphorylase, PNPase
3	View Details	[283..432]	MSA	4.179995	View MSA. No confident structure predictions are available.
4	View Details	[433..661]	PSI-BLAST	9.154902	RecG, N-terminal domain; RecG "wedge" domain; RecG helicase domain
5	View Details	[662..833]	PSI-BLAST	9.154902	RecG, N-terminal domain; RecG "wedge" domain; RecG helicase domain
6	View Details	[834..1145]	MSA	12.325996	View MSA. No confident structure predictions are available.

Protein Overview: PRP22

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: