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Protein Overview: FUN12

General Information:
Name(s) found: FUN12 / YAL035W [SGD]
Description(s) found:
Found 23 descriptions.
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  • GTPase, required for general translation initiation by promoting Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; homolog of bacterial IF2 [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 1002 amino acids

    Gene Ontology:
    Cellular Component: mitochondrion [IDALink to PubMed articleLink to PubMed article]
    Biological Process: translational initiation [IDALink to PubMed article]
    Molecular Function: translation initiation factor activity [IDALink to PubMed article]
    GTPase activity [IMPLink to PubMed article][ISSLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAKKSKKNQQ NYWDEEFEED AAQNEEISAT PTPNPESSAG ADDTSREASA SAEGAEAIEG  60
   61 DFMSTLKQSK KKQEKKVIEE KKDGKPILKS KKEKEKEKKE KEKQKKKEQA ARKKAQQQAQ 120
  121 KEKNKELNKQ NVEKAAAEKA AAEKSQKSKG ESDKPSASAK KPAKKVPAGL AALRRQLELK 180
  181 KQLEEQEKLE REEEERLEKE EEERLANEEK MKEEAKAAKK EKEKAKREKR KAEGKLLTRK 240
  241 QKEEKKLLER RRAALLSSGN VKVAGLAKKD GEENKPKKVV YSKKKKRTTQ ENASEAIKSD 300
  301 SKKDSEVVPD DELKESEDVL IDDWENLALG DDDEEGTNEE TQESTASHEN EDQNQGEEEE 360
  361 EGEEEEEEEE ERAHVHEVAK STPAATPAAT PTPSSASPNK KDLRSPICCI LGHVDTGKTK 420
  421 LLDKIRQTNV QGGEAGGITQ QIGATYFPID AIKAKTKVMA EYEKQTFDVP GLLVIDTPGH 480
  481 ESFSNLRSRG SSLCNIAILV IDIMHGLEQQ TIESIKLLRD RKAPFVVALN KIDRLYDWKA 540
  541 IPNNSFRDSF AKQSRAVQEE FQSRYSKIQL ELAEQGLNSE LYFQNKNMSK YVSIVPTSAV 600
  601 TGEGVPDLLW LLLELTQKRM SKQLMYLSHV EATILEVKVV EGFGTTIDVI LSNGYLREGD 660
  661 RIVLCGMNGP IVTNIRALLT PQPLRELRLK SEYVHHKEVK AALGVKIAAN DLEKAVSGSR 720
  721 LLVVGPEDDE DELMDDVMDD LTGLLDSVDT TGKGVVVQAS TLGSLEALLD FLKDMKIPVM 780
  781 SIGLGPVYKR DVMKASTMLE KAPEYAVMLC FDVKVDKEAE QYAEQEGIKI FNADVIYHLF 840
  841 DSFTAYQEKL LEERRKDFLD YAIFPCVLQT LQIINKRGPM IIGVDVLEGT LRVGTPICAV 900
  901 KTDPTTKERQ TLILGKVISL EINHQPVQEV KKGQTAAGVA VRLEDPSGQQ PIWGRHVDEN 960
  961 DTLYSLVSRR SIDTLKDKAF RDQVARSDWL LLKKLKVVFG IE

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) AVO1 CBF5 DBP2 ECM16 FUN12 HCR1 IMD1 IMD3 IMD4 KEM1 KRE33 MAK5 MIS1 NIP1 NPL3 PRP43 PRT1 PUF6 RBG1 RPA49 RPF2 RPG1 RPL15A RPL20B RPL2A, RPL2B RPL4B RPL5 RPL6A RPP0 RPS11A, RPS11B RPS1A RPS1B RPS3 RPS4B, RPS4A RPS6A, RPS6B RPS7A RPS8B, RPS8A STM1 TIF34 TIF35 TIF5 TOP2 UTP22 YGR054W
    View Details Gavin AC, et al. (2006) FUN12 HCR1 NIP1 PRT1 RLI1 RPG1 RPL12B, RPL12A TIF34 TIF35 TIF5
    View Details Gavin AC, et al. (2002) AAR2 ARB1 ARX1 ASC1 ATG9 BRX1 CBF5 CIC1 CLU1 DBP9 DRS1 ERB1 FUN12 GAR1 GLT1 HAS1 HCR1 HHF1, HHF2 IST2 KRE33 KRR1 KTR5 LHP1 LSG1 MAK21 MDN1 MIS1 MRP7 NHP2 NIP1 NOC2 NOG1 NOP1 NOP12 NOP2 NOP4 NOP56 NOP6 NOP7 NUG1 POL2 PRP4 PRT1 PUF6 RIC1 RLI1 RLP7 RPG1 RRP12 SMB1 SNU114 SNU66 SPT5 SRO9 SUA7 SUI1 SUI2 SUI3 SUP45 TIF34 TIF35 TIF4631 TIF4632 TIF5 URB1 UTP10 UTP20 UTP21 UTP6 VMA13 YGL036W YGR102C YMR310C YTM1
    View Details Ho Y, et al. (2002) AI1 AIM46 ARE1 ATP3 AVO1 CCT5 CDC33 CLU1 DHH1 DPB2 FET4 FUN12 GAL7 GPH1 GRS1 HAS1 HHO1 IDH1 ILV2 IMD2 IMD3 IMD4 KRE33 KRI1 MAG1 MAG2 MET16 MGM101 MKK2 MPH1 MSC3 MSH2 NOC2 NOG2 NOP12 NOP4 NPA3 NPL3 OYE2 PDC6 PDI1 PDX1 PHO85 PUB1 PUF3 RET1 RPC82 RPF2 RPN12 RRP12 SGN1 SIS1 SLX1 SPT2 SRP54 STI1 TEM1 TFC7 TIF4631 TIF4632 UTP22 VMA8 YER077C YGR250C YGR266W YKR051W YKU80 YLR271W YPR003C
    View Details Qiu et al. (2008) FUN12 GCD11 NIP1 PRT1 RPG1 SUI3 TIF3 TIF34 TIF35 TIF5 YGR054W

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run TID3 No Comments Cheeseman IM, et al. (2002)
    View Run HSL7 No Comments McCusker D, et al (2007)
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run RLF2 cac1: TAP-tagged Green EM, et al (2005)
    View Run RLF2 phosphorylation data - cascade search Green EM, et al (2005)
    View Run RLF2 identification data - first search in cascade Green EM, et al (2005)
    View Run BOI1 Sample bob1 (2nd set) from october 2005 McCusker D, et al (2007)
    View Run MLP2 #25 Asynchronous Prep3-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #31 Asynchronous Prep (Protease cleavage) Keck JM, et al. (2011)
    View Run MLP2 #29 Asynchronous Prep5-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #13 Mitotic Prep2-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #02 Alpha Factor Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)
    View Run MLP2 #11 Mitotic Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data FUN12 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..304] PSI-BLAST 3.09691 Heavy meromyosin subfragment
    2 View Details [305..385] deduced N/A No confident structure predictions are available.
    3 View Details [386..623] PSI-BLAST 1064.0 Initiation factor IF2/eIF5b, domains 2 and 4; Initiation factor IF2/eIF5b, domain 3; Initiation factor IF2/eIF5b, N-terminal (G) domain
    4 View Details [624..741] PSI-BLAST 1064.0 Initiation factor IF2/eIF5b, domains 2 and 4; Initiation factor IF2/eIF5b, domain 3; Initiation factor IF2/eIF5b, N-terminal (G) domain
    5 View Details [742..854] PSI-BLAST 1064.0 Initiation factor IF2/eIF5b, domains 2 and 4; Initiation factor IF2/eIF5b, domain 3; Initiation factor IF2/eIF5b, N-terminal (G) domain
    6 View Details [855..1002] PSI-BLAST 1064.0 Initiation factor IF2/eIF5b, domains 2 and 4; Initiation factor IF2/eIF5b, domain 3; Initiation factor IF2/eIF5b, N-terminal (G) domain

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2 No functions predicted.
    3
    Term Confidence Notes
  • translation initiation factor activity
    		•  4.55032413246022 bayes_pls_golite062009
  • catalytic activity
    		•  1.37148167194523 bayes_pls_golite062009
  • nucleic acid binding
    		•  0.993540749660144 bayes_pls_golite062009
  • binding
    		•  0.923764328759134 bayes_pls_golite062009
  • translation regulator activity
    		•  0.92120490618839 bayes_pls_golite062009
  • translation factor activity, nucleic acid binding
    		•  0.91253097238055 bayes_pls_golite062009
  • purine ribonucleotide binding
    		•  0.16063798469602 bayes_pls_golite062009
  • ribonucleotide binding
    		•  0.16015711008109 bayes_pls_golite062009
  • protein binding
    		•  0.153255883232138 bayes_pls_golite062009
  • purine nucleotide binding
    		•  0.146890053953086 bayes_pls_golite062009
  • nucleotide binding
    		•  0.136564780426908 bayes_pls_golite062009
  • DNA binding
    		•  0.128213991729785 bayes_pls_golite062009
  • transcription regulator activity
    		•  0.125640198783258 bayes_pls_golite062009
    4 No functions predicted.
    5 No functions predicted.
    6 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle