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Protein Overview: GLT1

General Information:
Name(s) found: GLT1 / YDL171C [SGD]
Description(s) found:
Found 27 descriptions.
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  • NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 2145 amino acids

    Gene Ontology:
    Cellular Component: mitochondrion [IDALink to PubMed articleLink to PubMed article]
    glutamate synthase complex [IPILink to PubMed article]
    Biological Process: glutamate biosynthetic process [IEPLink to PubMed article]
    Molecular Function: glutamate synthase (NADH) activity [ISSLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPVLKSDNFD PLEEAYEGGT IQNYNDEHHL HKSWANVIPD KRGLYDPDYE HDACGVGFVA  60
   61 NKHGEQSHKI VTDARYLLVN MTHRGAVSSD GNGDGAGILL GIPHEFMKRE FKLDLDLDIP 120
  121 EMGKYAVGNV FFKKNEKNNK KNLIKCQKIF EDLAASFNLS VLGWRNVPVD STILGDVALS 180
  181 REPTILQPLL VPLYDEKQPE FNETKFRTQL YLLRKEASLQ IGLENWFYVC SLNNTTIVYK 240
  241 GQLTPAQVYN YYPDLTNAHF KSHMALVHSR FSTNTFPSWD RAQPLRWLAH NGEINTLRGN 300
  301 KNWMRSREGV MNSATFKDEL DKLYPIIEEG GSDSAALDNV LELLTINGTL SLPEAVMMMV 360
  361 PEAYHKDMDS DLKAWYDWAA CLMEPWDGPA LLTFTDGRYC GAILDRNGLR PCRYYITSDD 420
  421 RVICASEVGV IPIENSLVVQ KGKLKPGDLF LVDTQLGEMV DTKKLKSQIS KRQDFKSWLS 480
  481 KVIKLDDLLS KTANLVPKEF ISQDSLSLKV QSDPRLLANG YTFEQVTFLL TPMALTGKEA 540
  541 LGSMGNDAPL ACLNENPVLL YDYFRQLFAQ VTNPPIDPIR EANVMSLECY VGPQGNLLEM 600
  601 HSSQCDRLLL KSPILHWNEF QALKNIEAAY PSWSVAEIDI TFDKSEGLLG YTDTIDKITK 660
  661 LASEAIDDGK KILIITDRKM GANRVSISSL IAISCIHHHL IRNKQRSQVA LILETGEARE 720
  721 IHHFCVLLGY GCDGVYPYLA METLVRMNRE GLLRNVNNDN DTLEEGQILE NYKHAIDAGI 780
  781 LKVMSKMGIS TLASYKGAQI FEALGLDNSI VDLCFTGTSS RIRGVTFEYL AQDAFSLHER 840
  841 GYPSRQTISK SVNLPESGEY HFRDGGYKHV NEPTAIASLQ DTVRNKNDVS WQLYVKKEME 900
  901 AIRDCTLRGL LELDFENSVS IPLEQVEPWT EIARRFASGA MSYGSISMEA HSTLAIAMNR 960
  961 LGAKSNCGEG GEDAERSAVQ ENGDTMRSAI KQVASARFGV TSYYLSDADE IQIKIAQGAK1020
 1021 PGEGGELPAH KVSKDIAKTR HSTPNVGLIS PPPHHDIYSI EDLKQLIYDL KCANPRAGIS1080
 1081 VKLVSEVGVG IVASGVAKAK ADHILVSGHD GGTGAARWTS VKYAGLPWEL GLAETHQTLV1140
 1141 LNDLRRNVVV QTDGQLRTGF DIAVAVLLGA ESFTLATVPL IAMGCVMLRR CHLNSCAVGI1200
 1201 ATQDPYLRSK FKGQPEHVIN FFYYLIQDLR QIMAKLGFRT IDEMVGHSEK LKKRDDVNAK1260
 1261 AINIDLSPIL TPAHVIRPGV PTKFTKKQDH KLHTRLDNKL IDEAEVTLDR GLPVNIDASI1320
 1321 INTDRALGST LSYRVSKKFG EDGLPKDTVV VNIEGSAGQS FGAFLASGIT FILNGDANDY1380
 1381 VGKGLSGGII VIKPPKDSKF KSDENVIVGN TCFYGATSGT AFISGSAGER FGVRNSGATI1440
 1441 VVERIKGNNA FEYMTGGRAI VLSQMESLNA FSGATGGIAY CLTSDYDDFV GKINKDTVEL1500
 1501 ESLCDPVEIA FVKNLIQEHW NYTQSDLAAR ILGNFNHYLK DFVKVIPTDY KKVLLKEKAE1560
 1561 AAKAKAKATS EYLKKFRSNQ EVDDEVNTLL IANQKAKEQE KKKSITISNK ATLKEPKVVD1620
 1621 LEDAVPDSKQ LEKNSERIEK TRGFMIHKRR HETHRDPRTR VNDWKEFTNP ITKKDAKYQT1680
 1681 ARCMDCGTPF CLSDTGCPLS NIIPKFNELL FKNQWKLALD KLLETNNFPE FTGRVCPAPC1740
 1741 EGACTLGIIE DPVGIKSVER IIIDNAFKEG WIKPCPPSTR TGFTVGVIGS GPAGLACADM1800
 1801 LNRAGHTVTV YERSDRCGGL LMYGIPNMKL DKAIVQRRID LLSAEGIDFV TNTEIGKTIS1860
 1861 MDELKNKHNA VVYAIGSTIP RDLPIKGREL KNIDFAMQLL ESNTKALLNK DLEIIREKIQ1920
 1921 GKKVIVVGGG DTGNDCLGTS VRHGAASVLN FELLPEPPVE RAKDNPWPQW PRVMRVDYGH1980
 1981 AEVKEHYGRD PREYCILSKE FIGNDEGEVT AIRTVRVEWK KSQSGVWQMV EIPNSEEIFE2040
 2041 ADIILLSMGF VGPELINGND NEVKKTRRGT IATLDDSSYS IDGGKTFACG DCRRGQSLIV2100
 2101 WAIQEGRKCA ASVDKFLMDG TTYLPSNGGI VQRDYKLLKE LASQV

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Gavin AC, et al. (2006) GLT1 RPL10 RPS5
    View Details Gavin AC, et al. (2002) ASC1 ATG9 BCP1 CDC45 CLU1 ERG26 FUN12 GFA1 GLT1 HAT1 HCR1 HSP42 IST2 KAP123 KTR5 LSG1 LYS12 MCM2 MCM5 MCM6 MCM7 NIP1 NOC2 NOG1 NOP7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 PRT1 RIC1 RKM1 RLI1 RPD3 RPG1 RPN9 SIN3 SPT5 SSA4 SUA7 SUI1 SUI2 SUI3 SUP45 TFP1 TIF34 TIF35 TIF5 UTP6 YGL036W YOL098C
    View Details Ho Y, et al. (2002) ACC1 ARP2 BGL2 CCT6 FAA1 FAA4 GCD11 GLT1 GSF2 MAE1 MET18 NDE1 NPA3 RMT2 RNR2 RPT4 SAP155 SAP185 SAP190 SIT4 SPC24 SPC25 TAP42 YMR196W

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run SEC10 No Comments De Craene, J., et al. (2006)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run ASF1 3912: TAP-tagged, strain background includes hpc2(delta) Green EM, et al (2005)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run MLP2 #25 Asynchronous Prep3-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #29 Asynchronous Prep5-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #11 Mitotic Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data GLT1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..499] PSI-BLAST 11000.0 Alpha subunit of glutamate synthase, C-terminal domain; Alpha subunit of glutamate synthase, central and FMN domains; Alpha subunit of glutamate synthase, N-terminal domain
    2 View Details [500..856] PSI-BLAST 11000.0 Alpha subunit of glutamate synthase, C-terminal domain; Alpha subunit of glutamate synthase, central and FMN domains; Alpha subunit of glutamate synthase, N-terminal domain
    3 View Details [857..1292] PSI-BLAST 11000.0 Alpha subunit of glutamate synthase, C-terminal domain; Alpha subunit of glutamate synthase, central and FMN domains; Alpha subunit of glutamate synthase, N-terminal domain
    4 View Details [1293..1608] PSI-BLAST 11000.0 Alpha subunit of glutamate synthase, C-terminal domain; Alpha subunit of glutamate synthase, central and FMN domains; Alpha subunit of glutamate synthase, N-terminal domain
    5 View Details [1609..2145] PSI-BLAST 259.9897 Dihydropyrimidine dehydrogenase, N-terminal domain; Dihydropyrimidine dehydrogenase, domain 4; Dihydropyrimidine dehydrogenase, domain 3; Dihydropyrimidine dehydrogenase, domain 2; Dihydropyrimidine dehydrogenase, C-terminal domain

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • glutamate synthase activity
    		•  10.2123323896554 bayes_pls_golite062009
  • glutamate synthase activity, NADH or NADPH as acceptor
    		•  9.64220719720108 bayes_pls_golite062009
  • glutamate synthase (NADPH) activity
    		•  7.71666213946687 bayes_pls_golite062009
  • oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor
    		•  3.97793872368585 bayes_pls_golite062009
  • oxidoreductase activity
    		•  3.55520438339582 bayes_pls_golite062009
  • oxidoreductase activity, acting on the CH-CH group of donors
    		•  1.75223963289059 bayes_pls_golite062009
  • catalytic activity
    		•  1.19663085909195 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.07596988808091 bayes_pls_golite062009
  • endopeptidase activity
    		•  1.05931638234802 bayes_pls_golite062009
  • peptidase activity, acting on L-amino acid peptides
    		•  0.821264875808897 bayes_pls_golite062009
  • peptidase activity
    		•  0.755649562279406 bayes_pls_golite062009
  • binding
    		•  0.752066499785082 bayes_pls_golite062009
  • glutamate synthase (NADH) activity
    		•  0.589012306271491 bayes_pls_golite062009
  • oxidoreductase activity, acting on the CH-NH2 group of donors
    		•  0.391558094818592 bayes_pls_golite062009
  • transferase activity, transferring acyl groups
    		•  0.181206025480438 bayes_pls_golite062009
    2
    Term Confidence Notes
  • glutamate synthase activity
    		•  8.25305391467034 bayes_pls_golite062009
  • glutamate synthase activity, NADH or NADPH as acceptor
    		•  8.23578981764913 bayes_pls_golite062009
  • glutamate synthase (NADPH) activity
    		•  6.79992270958953 bayes_pls_golite062009
  • oxidoreductase activity
    		•  3.19495080984613 bayes_pls_golite062009
  • oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor
    		•  2.59457345868059 bayes_pls_golite062009
  • succinate dehydrogenase activity
    		•  1.80846474206463 bayes_pls_golite062009
  • catalytic activity
    		•  1.56773888754354 bayes_pls_golite062009
  • binding
    		•  0.993436593091194 bayes_pls_golite062009
  • oxidoreductase activity, acting on sulfur group of donors
    		•  0.704326257168965 bayes_pls_golite062009
  • oxidoreductase activity, acting on sulfur group of donors, NAD or NADP as acceptor
    		•  0.242456601435627 bayes_pls_golite062009
  • succinate dehydrogenase (ubiquinone) activity
    		•  0.228954938673819 bayes_pls_golite062009
  • oxidoreductase activity, acting on the CH-CH group of donors, quinone or related compound as acceptor
    		•  0.228954938673819 bayes_pls_golite062009
  • protein binding
    		•  0.116927178396589 bayes_pls_golite062009
    3 No functions predicted.
    4 No functions predicted.
    5 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.94

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle