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Run Information:

Bait Protein: ASF1
Organism: Saccharomyces cerevisiae
Publication: Green EM, Antczak AJ, Bailey AO, Franco AA, Wu KJ, Yates JR 3rd, Kaufman PD. Replication-independent histone deposition by the HIR complex and Asf1. Curr Biol. 2005 Nov 22;15(22):2044-9.
Comments: 3912: TAP-tagged, strain background includes hpc2(delta)

Raw Data:

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   ASF1 Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein t... 78 1394 70.3% 0.716 31603
[View Peptides]   RAD53 Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autopho... 90 362 63.9% 0.0632 91962
[View Peptides]   SHM1 Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahy... 8 8 26.1% 0.00234 53686
[View Peptides]   GPM1 Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycer... 3 3 25.9% 0.00174 27609
[View Peptides]   PDC1 Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... 9 16 20.8% 0.00407 61495
[View Peptides]   ENO2 Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... 6 7 20.6% 0.0023 46914
[View Peptides]   HHF1, HHF2 Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two... 3 7 18.4% 0.00974 11368
[View Peptides]   PGK1 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phospha... 4 4 14.4% 0.00138 44738
[View Peptides]   YDJ1 Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein trans... 2 2 13.4% 7.01E-4 44671
[View Peptides]   ENO1 Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosph... 4 5 12.8% 0.00164 46816
[View Peptides]   IST1 Protein with a positive role in the multivesicular body sorting pathway; functions and forms a compl... 2 2 10.1% 9.62E-4 34496
[View Peptides]   CYS4 Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the ... 2 2 8.5% 5.66E-4 56022
[View Peptides]   SAM1 S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom ... 2 2 8.4% 7.51E-4 41818
[View Peptides]   SAM2 S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom ... 2 2 8.3% 7.47E-4 42256
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 4 5 8.2% 0.00143 54545
[View Peptides]   YHR054C Putative protein of unknown function 2 2 8.2% 8.1E-4 41061
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 2 2 8.1% 6.26E-4 50033
[View Peptides]   GLO4 Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and... 2 2 7.4% 0.00101 32339
[View Peptides]   PAP2 Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP co... 2 2 6.7% 4.91E-4 66031
[View Peptides]   CYS3 Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathw... 2 2 6.6% 7.28E-4 42542
[View Peptides]   ADH1 Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the red... 2 2 6.6% 8.24E-4 36849
[View Peptides]   SPG5 Protein required for survival at high temperature during stationary phase; not required for growth o... 2 2 6.4% 7.69E-4 42163
[View Peptides]   HSP60 Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides... 2 2 5.4% 5.01E-4 60752
[View Peptides]   ADP1 Putative ATP-dependent permease of the ABC transporter family of proteins 2 3 4.9% 4.1E-4 117231
[View Peptides]   MSC6 Protein of unknown function; mutant is defective in directing meiotic recombination events to homolo... 2 2 4.6% 4.14E-4 79947
[View Peptides]   DSL1 Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p a... 3 4 4.2% 7.61E-4 88072
[View Peptides]   PDC5 Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruva... 2 3 3.9% 7.64E-4 61912
[View Peptides]   VPS34 Phosphatidylinositol 3-kinase responsible for the synthesis of phosphatidylinositol 3-phosphate; for... 2 2 3.8% 3.28E-4 100921
[View Peptides]   PIB2 Protein binding phosphatidylinositol 3-phosphate, involved in telomere-proximal repression of gene e... 2 2 3.8% 4.52E-4 70617
[View Peptides]   YBP2 Central kinetochore associated protein that mediates mitotic progression; interacts with several cen... 2 3 3.7% 6.71E-4 73102
[View Peptides]   TIF4631 Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that al... 2 2 3.6% 3.01E-4 107101
[View Peptides]   LHS1 Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and fo... 2 2 3.6% 3.25E-4 99572
[View Peptides]   RSC30 Component of the RSC chromatin remodeling complex; non-essential gene required for regulation of rib... 2 2 3.5% 3.45E-4 95520
[View Peptides]   SFB2 Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat, required for cargo selection du... 2 8 3.3% 0.00131 98943
[View Peptides]   ECM10 Heat shock protein of the Hsp70 family, localized in mitochondrial nucleoids, plays a role in protei... 2 3 3.3% 6.68E-4 70085
[View Peptides]   SSC1 Hsp70 family ATPase, constituent of the import motor component of the Translocase of the Inner Mitoc... 2 2 3.2% 4.38E-4 70628
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 2 3 3.1% 6.31E-4 74468
[View Peptides]   APL2 Beta-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; invol... 2 4 2.9% 7.9E-4 81869
[View Peptides]   SSM4 Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear en... 2 4 2.6% 4.35E-4 151454
[View Peptides]   GDE1 Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphospha... 2 20 2.3% 0.00234 138014
[View Peptides]   GLT1 NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglut... 2 3 2.2% 2.0E-4 238100
[View Peptides]   YLR001C Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purifi... 3 23 2.2% 0.00383 97618
[View Peptides]   NUP159 Nucleoporin, subunit of the nuclear pore complex that is found exclusively on the cytoplasmic side, ... 2 2 2.1% 1.96E-4 158907
[View Peptides]   YBR235W Putative ion transporter, similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family; Y... 2 2 2.1% 2.56E-4 123998
[View Peptides]   MUC1 GPI-anchored cell surface glycoprotein (flocculin) required for pseudohyphal formation, invasive gro... 2 4 1.7% 4.19E-4 136110
[View Peptides]   TOR2 PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth i... 2 2 1.4% 1.16E-4 281509
[View Peptides]   URA2 Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes t... 2 2 1.4% 1.3E-4 245123
[View Peptides]   MDM20 Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of pro... 2 2 1.1% 3.6E-4 92809
[View Peptides]   TOM1 E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may reg... 2 4 1.0% 1.75E-4 374186
[View Peptides]   BLM10 Proteasome activator subunit; found in association with core particles, with and without the 19S reg... 2 3 1.0% 2.01E-4 245993

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