Protein Overview: URA2

General Information:
Name(s) found:	URA2 / YJL130C [SGD]
Description(s) found: Found 22 descriptions. SHOW ALL	Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP [SGD]
Organism:	Saccharomyces cerevisiae
Length:	2214 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA] mitochondrion [IDA ] integral to membrane [IDA]
Biological Process:	'de novo' pyrimidine base biosynthetic process [TAS] glutamine metabolic process [IDA] pyrimidine base biosynthetic process [IDA]
Molecular Function:	aspartate carbamoyltransferase activity [IDA] carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MATIAPTAPI TPPMESTGDR LVTLELKDGT VLQGYSFGAE KSVAGELVFQ TGMVGYPESV  60
   61 TDPSYEGQIL VITYPLVGNY GVPDMHLRDE LVEELPRYFE SNRIHIAGLV ISHYTDEYSH 120
  121 YLRKSSLGKW LQNEGIPAVY GVDTRSLTKH LRDAGSMLGR LSLEKSGSDR TISRSSSWRS 180
  181 AFDVPEWVDP NVQNLVSKVS INEPKLYVPP ADNKHIELQT GPDGKVLRIL AIDVGMKYNQ 240
  241 IRCFIKRGVE LKVVPWNYDF TKEDYDGLFI SNGPGDPSVL DDLSQRLSNV LEAKKTPVFG 300
  301 ICLGHQLIAR AAGASTLKLK FGNRGHNIPC TSTISGRCYI TSQNHGFAVD VDTLTSGWKP 360
  361 LFVNANDDSN EGIYHSELPY FSVQFHPEST PGPRDTEFLF DVFIQAVKEF KYTQVLKPIA 420
  421 FPGGLLEDNV KAHPRIEAKK VLVLGSGGLS IGQAGEFDYS GSQAIKALKE EGIYTILINP 480
  481 NIATIQTSKG LADKVYFVPV TAEFVRKVIL HERPDAIYVT FGGQTALSVG IAMKDEFEAL 540
  541 GVKVLGTPID TIITTEDREL FSNAIDEINE KCAKSQAANS VDEALAAVKE IGFPVIVRAA 600
  601 YALGGLGSGF ANNEKELVDL CNVAFSSSPQ VLVEKSMKGW KEVEYEVVRD AFDNCITVCN 660
  661 MENFDPLGIH TGDSIVVAPS QTLSDEDYNM LRTTAVNVIR HLGVVGECNI QYALNPVSKD 720
  721 YCIIEVNARL SRSSALASKA TGYPLAYTAA KLGLNIPLNE VKNSVTKSTC ACFEPSLDYC 780
  781 VVKMPRWDLK KFTRVSTELS SSMKSVGEVM SIGRTFEEAI QKAIRSTEYA NLGFNETDLD 840
  841 IDIDYELNNP TDMRVFAIAN AFAKKGYSVD KVWEMTRIDK WFLNKLHDLV QFAEKISSFG 900
  901 TKEELPSLVL RQAKQLGFDD RQIARFLDSN EVAIRRLRKE YGITPFVKQI DTVAAEFPAY 960
  961 TNYLYMTYNA DSHDLSFDDH GVMVLGSGVY RIGSSVEFDW CAVTAVRTLR ANNIKTIMVN1020
 1021 YNPETVSTDY DEADRLYFET INLERVLDIY EIENSSGVVV SMGGQTSNNI AMTLHRENVK1080
 1081 ILGTSPDMID SAENRYKFSR MLDQIGVDQP AWKELTSMDE AESFAEKVGY PVLVRPSYVL1140
 1141 SGAAMNTVYS KNDLESYLNQ AVEVSRDYPV VITKYIENAK EIEMDAVARN GELVMHVVSE1200
 1201 HVENAGVHSG DATLIVPPQD LAPETVDRIV VATAKIGKAL KITGPYNIQF IAKDNEIKVI1260
 1261 ECNVRASRSF PFISKVVGVN LIELATKAIM GLPLTPYPVE KLPDDYVAVK VPQFSFPRLA1320
 1321 GADPVLGVEM ASTGEVATFG HSKYEAYLKS LLATGFKLPK KNILLSIGSY KEKQELLSSV1380
 1381 QKLYNMGYKL FATSGTADFL SEHGIAVQYL EVLNKDDDDQ KSEYSLTQHL ANNEIDLYIN1440
 1441 LPSANRFRRP ASYVSKGYKT RRLAVDYSVP LVTNVKCAKL LIEAISRNIT LDVSERDAQT1500
 1501 SHRTITLPGL INIATYVPNA SHVIKGPAEL KETTRLFLES GFTYCQLMPR SISGPVITDV1560
 1561 ASLKAANSVS QDSSYTDFSF TIAGTAHNAH SVTQSASKVT ALFLPLRELK NKITAVAELL1620
 1621 NQWPTEKQVI AEAKTADLAS VLLLTSLQNR SIHITGVSNK EDLALIMTVK AKDPRVTCDV1680
 1681 NIYSLFIAQD DYPEAVFLPT KEDQEFFWNN LDSIDAFSVG ALPVALANVT GNKVDVGMGI1740
 1741 KDSLPLLLAA VEEGKLTIDD IVLRLHDNPA KIFNIPTQDS VVEIDLDYSF RRNKRWSPFN1800
 1801 KDMNGGIERV VYNGETLVLS GELVSPGAKG KCIVNPSPAS ITASAELQST SAKRRFSITE1860
 1861 EAIADNLDAA EDAIPEQPLE QKLMSSRPPR ELVAPGAIQN LIRSNNPFRG RHILSIKQFK1920
 1921 RSDFHVLFAV AQELRAAVAR EGVLDLMKGH VITTIFFEPS TRTCSSFIAA MERLGGRIVN1980
 1981 VNPLVSSVKK GETLQDTIRT LACYSDAIVM RHSEEMSVHI AAKYSPVPII NGGNGSREHP2040
 2041 TQAFLDLFTI REEIGTVNGI TVTFMGDLKH GRTVHSLCRL LMHYQVRINL VSPPELRLPE2100
 2101 GLREELRKAG LLGVESIELT PHIISKTDVL YCTRVQEERF NSPEEYARLK DTYIVDNKIL2160
 2161 AHAKENMAIM HPLPRVNEIK EEVDYDHRAA YFRQMKYGLF VRMALLAMVM GVDM

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		NPL3 RPS0B RPS4B, RPS4A URA2 VMA2 YDJ1
View Details	Krogan NJ, et al. (2006)		CPA1 CPA2 URA2

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	KIP3	No Comments	Cheeseman IM, et al. (2002)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	ASF1	3912: TAP-tagged, strain background includes hpc2(delta)	Green EM, et al (2005)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	RLF2	phosphorylation data - cascade search	Green EM, et al (2005)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi1 with gst from october 2005	McCusker D, et al (2007)
View Run	BOI1	Sample bob1 (2nd set) from october 2005	McCusker D, et al (2007)
View Run	VAM3	Sample: HX11 - both fusion and trans-SNARE complex formation take place.	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control).	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX14 - mixture in detergent (control).	Hao Xu, et al. (2010)
View Run	VAM3	hx23: mixture in detergent (control) Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex	Hao Xu, et al. (2010)
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#13 Mitotic Prep2-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#02 Alpha Factor Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..190]	PSI-BLAST	764.0	Carbamoyl phosphate synthetase, small subunit N-terminal domain; Carbamoyl phosphate synthetase, small subunit C-terminal domain
2	View Details	[191..422]	PSI-BLAST	764.0	Carbamoyl phosphate synthetase, small subunit N-terminal domain; Carbamoyl phosphate synthetase, small subunit C-terminal domain
3	View Details	[423..573] [637..779]	PSI-BLAST	11000.0	Carbamoyl phosphate synthetase, large subunit connection domain; Carbamoyl phosphate synthetase, large subunit allosteric, C-terminal domain; Carbamoyl phosphate synthetase (CPS), large subunit PreATP-grasp domains; Carbamoyl phosphate synthetase (CPS), large subunit ATP-binding domains
4	View Details	[574..636] [780..890]	PSI-BLAST	11000.0	Carbamoyl phosphate synthetase, large subunit connection domain; Carbamoyl phosphate synthetase, large subunit allosteric, C-terminal domain; Carbamoyl phosphate synthetase (CPS), large subunit PreATP-grasp domains; Carbamoyl phosphate synthetase (CPS), large subunit ATP-binding domains
5	View Details	[891..964]	PSI-BLAST	11000.0	Carbamoyl phosphate synthetase, large subunit connection domain; Carbamoyl phosphate synthetase, large subunit allosteric, C-terminal domain; Carbamoyl phosphate synthetase (CPS), large subunit PreATP-grasp domains; Carbamoyl phosphate synthetase (CPS), large subunit ATP-binding domains
6	View Details	[965..1086]	PSI-BLAST	11000.0	Carbamoyl phosphate synthetase, large subunit connection domain; Carbamoyl phosphate synthetase, large subunit allosteric, C-terminal domain; Carbamoyl phosphate synthetase (CPS), large subunit PreATP-grasp domains; Carbamoyl phosphate synthetase (CPS), large subunit ATP-binding domains
7	View Details	[1087..1110] [1176..1356] [1484..1490]	PSI-BLAST	11000.0	Carbamoyl phosphate synthetase, large subunit connection domain; Carbamoyl phosphate synthetase, large subunit allosteric, C-terminal domain; Carbamoyl phosphate synthetase (CPS), large subunit PreATP-grasp domains; Carbamoyl phosphate synthetase (CPS), large subunit ATP-binding domains
8	View Details	[1111..1175] [1357..1483]	PSI-BLAST	11000.0	Carbamoyl phosphate synthetase, large subunit connection domain; Carbamoyl phosphate synthetase, large subunit allosteric, C-terminal domain; Carbamoyl phosphate synthetase (CPS), large subunit PreATP-grasp domains; Carbamoyl phosphate synthetase (CPS), large subunit ATP-binding domains
9	View Details	[1491..1837]	PSI-BLAST	32.0	L-hydantoinase
10	View Details	[1838..1897]	deduced	N/A	No confident structure predictions are available.
11	View Details	[1898..1946] [2034..2046] [2195..2214]	PSI-BLAST	717.9691	Aspartate carbamoyltransferase catalytic subunit
12	View Details	[1947..2033] [2047..2194]	PSI-BLAST	717.9691	Aspartate carbamoyltransferase catalytic subunit

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity	11.6556846811261	bayes_pls_golite062009
carbamoyl-phosphate synthase activity	11.614541080865	bayes_pls_golite062009
carbon-nitrogen ligase activity, with glutamine as amido-N-donor	8.83104490344765	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	6.7523653122648	bayes_pls_golite062009
ligase activity	2.64230127203047	bayes_pls_golite062009
nucleic acid binding	1.83357525248647	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	1.68223120807504	bayes_pls_golite062009
kinase activity	1.67508566693087	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	1.57588199570083	bayes_pls_golite062009
transferase activity	1.56222597521764	bayes_pls_golite062009
phosphoribosylformylglycinamidine synthase activity	1.51568729563523	bayes_pls_golite062009
binding	1.22860407524238	bayes_pls_golite062009
catalytic activity	0.902495165806215	bayes_pls_golite062009
transferase activity, transferring glycosyl groups	0.774587372455935	bayes_pls_golite062009
hydrolase activity	0.488948677783382	bayes_pls_golite062009
imidazoleglycerol-phosphate synthase activity	0.270391651600004	bayes_pls_golite062009
anthranilate synthase activity	0.243205818526006	bayes_pls_golite062009
peptidase activity	0.0466975117232061	bayes_pls_golite062009
protein binding	0.0152024556625711	bayes_pls_golite062009

Term	Confidence	Notes
carbamoyl-phosphate synthase activity	10.3961907577933	bayes_pls_golite062009
carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity	10.3817460390594	bayes_pls_golite062009
carbon-nitrogen ligase activity, with glutamine as amido-N-donor	7.55690629692775	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	6.95559501787172	bayes_pls_golite062009
carbamoyl-phosphate synthase (ammonia) activity	5.29301695671381	bayes_pls_golite062009
aspartate carbamoyltransferase activity	3.38433995801364	bayes_pls_golite062009
ligase activity	3.1029847500488	bayes_pls_golite062009
dihydroorotase activity	2.87758723400434	bayes_pls_golite062009
succinate-CoA ligase activity	2.77725772893528	bayes_pls_golite062009
carboxyl- or carbamoyltransferase activity	2.46049425383361	bayes_pls_golite062009
acid-amino acid ligase activity	2.05746473175561	bayes_pls_golite062009
CoA-ligase activity	1.95069402824125	bayes_pls_golite062009
binding	1.94172111366073	bayes_pls_golite062009
acid-thiol ligase activity	1.72672517234442	bayes_pls_golite062009
transferase activity	1.65470153555003	bayes_pls_golite062009
phosphoribosylaminoimidazole carboxylase activity	1.57708889650281	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	1.43253315277789	bayes_pls_golite062009
catalytic activity	1.08688404807699	bayes_pls_golite062009
phosphoribosylamine-glycine ligase activity	1.04915286326924	bayes_pls_golite062009
carbon-oxygen lyase activity, acting on phosphates	0.964679554894316	bayes_pls_golite062009
ligase activity, forming carbon-sulfur bonds	0.96165574211624	bayes_pls_golite062009
biotin carboxylase activity	0.925630424589009	bayes_pls_golite062009
phosphoribosylaminoimidazolecarboxamide formyltransferase activity	0.898425265043358	bayes_pls_golite062009
hydrolase activity	0.756177577540965	bayes_pls_golite062009
succinate-CoA ligase (ADP-forming) activity	0.731047220665703	bayes_pls_golite062009
carbon-oxygen lyase activity	0.482425669839603	bayes_pls_golite062009
protein binding	0.455938814211606	bayes_pls_golite062009
lyase activity	0.432948879967458	bayes_pls_golite062009
acetyl-CoA carboxylase activity	0.39538073184604	bayes_pls_golite062009
propionyl-CoA carboxylase activity	0.377204236873769	bayes_pls_golite062009
ATP binding	0.268802736141542	bayes_pls_golite062009
adenyl ribonucleotide binding	0.245910202001696	bayes_pls_golite062009
hydroxymethyl-, formyl- and related transferase activity	0.2431211195117	bayes_pls_golite062009
adenyl nucleotide binding	0.23797201509664	bayes_pls_golite062009
glutathione binding	0.20743435331623	bayes_pls_golite062009
nucleotide binding	0.106800787341606	bayes_pls_golite062009
ribonucleotide binding	0.105378337351009	bayes_pls_golite062009
purine nucleotide binding	0.105328960473132	bayes_pls_golite062009
purine ribonucleotide binding	0.105164298301833	bayes_pls_golite062009
glutathione synthase activity	0.0685848191991462	bayes_pls_golite062009
succinate-CoA ligase (GDP-forming) activity	0.04189990190488	bayes_pls_golite062009

Term	Confidence	Notes
dihydroorotase activity	5.83804402298952	bayes_pls_golite062009
hydrolase activity	3.43682651851542	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides	3.03591306120652	bayes_pls_golite062009
transcription regulator activity	2.05422491012934	bayes_pls_golite062009
deaminase activity	1.79186203771221	bayes_pls_golite062009
nucleic acid binding	1.75483241027992	bayes_pls_golite062009
catalytic activity	1.70199291046899	bayes_pls_golite062009
DNA binding	1.70075498563244	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	1.60895003474033	bayes_pls_golite062009
binding	1.20793368650496	bayes_pls_golite062009
transcription factor activity	1.01581417842484	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines	0.573107361273483	bayes_pls_golite062009
deacetylase activity	0.57075565732108	bayes_pls_golite062009
guanine deaminase activity	0.134430884998957	bayes_pls_golite062009
dihydropyrimidinase activity	0.101722148781441	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
carboxyl- or carbamoyltransferase activity	8.44774290170513	bayes_pls_golite062009
carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity	8.39877026738977	bayes_pls_golite062009
carbamoyl-phosphate synthase activity	8.35868658131065	bayes_pls_golite062009
aspartate carbamoyltransferase activity	7.47628089321489	bayes_pls_golite062009
ornithine carbamoyltransferase activity	6.08861672645671	bayes_pls_golite062009
carbon-nitrogen ligase activity, with glutamine as amido-N-donor	5.70428077078256	bayes_pls_golite062009
transferase activity	4.46011936943199	bayes_pls_golite062009
dihydroorotase activity	4.10312514971972	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	4.09429261864362	bayes_pls_golite062009
aspartate binding	3.43574933938267	bayes_pls_golite062009
UTP binding	3.43574933938267	bayes_pls_golite062009
transferase activity, transferring one-carbon groups	2.1749430001471	bayes_pls_golite062009
catalytic activity	2.06069688716378	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	1.71477949033699	bayes_pls_golite062009
kinase activity	1.70283432187593	bayes_pls_golite062009
binding	1.63251597999268	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	1.5976562586299	bayes_pls_golite062009
protein kinase activity	1.48895685647086	bayes_pls_golite062009
hydrolase activity	0.628276058923848	bayes_pls_golite062009
ligase activity	0.345534785035194	bayes_pls_golite062009
amine binding	0.33918556173094	bayes_pls_golite062009
protein binding	0.271043612914069	bayes_pls_golite062009
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides	0.254873181665999	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

	PROTEIN(S)	PUBLICATION
View Data	URA2	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Protein Overview: URA2

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: