Enzymatic activities of Ura2 and Ura1 proteins (aspartate carbamoyltransferase and dihydro-orotate dehydrogenase) are present in both isolated membranes and cytoplasm of Saccharomyces cerevisiae

J Vorísek; Z Techniková; J Schwippel; P Benoist

doi:10.1002/yea.845

Enzymatic activities of Ura2 and Ura1 proteins (aspartate carbamoyltransferase and dihydro-orotate dehydrogenase) are present in both isolated membranes and cytoplasm of Saccharomyces cerevisiae

Yeast. 2002 Mar 30;19(5):449-57. doi: 10.1002/yea.845.

Authors

J Vorísek¹, Z Techniková, J Schwippel, P Benoist

Affiliation

¹ Institute of Microbiology, Academy of Sciences of Czech Republic, Vídenská 1083, CZ-14220 Praha 4-Krc, Czech Republic.

PMID: 11921093
DOI: 10.1002/yea.845

Abstract

Computational analysis predicted three potential hydrophobic transmembrane alpha-helices within the Ura2 multidomain protein of Saccharomyces cerevisiae, the C-terminal subdomain of which catalyses the second step of uridine-monophosphate biosynthesis by its L-aspartate carbamoyltransferase activity (EC 2.1.3.2). The fourth step of pyrimidine biosynthesis is catalysed by dihydro-orotate dehydrogenase (Ura1 protein; EC 1.3.99.11), which was similarly characterized as a peripheral membrane protein. Ex situ, the activities of the investigated enzymes were associated both with isolated yeast membranes, fractionated by differential centrifugation to remove intact nuclei, and with soluble cytoplasmic proteins.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Aspartate Carbamoyltransferase / biosynthesis
Aspartate Carbamoyltransferase / metabolism*
Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / biosynthesis
Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / metabolism*
Cell Membrane / enzymology
Cytoplasm / enzymology
Dihydroorotate Dehydrogenase
Fluorescent Dyes / chemistry
Indoles / chemistry
Molecular Sequence Data
Multienzyme Complexes / biosynthesis
Multienzyme Complexes / metabolism*
Oxidoreductases / biosynthesis
Oxidoreductases / metabolism*
Oxidoreductases Acting on CH-CH Group Donors*
Protein Structure, Secondary
Saccharomyces cerevisiae / enzymology*
Saccharomyces cerevisiae / physiology
Saccharomyces cerevisiae Proteins*

Substances

Dihydroorotate Dehydrogenase
Fluorescent Dyes
Indoles
Multienzyme Complexes
Saccharomyces cerevisiae Proteins
URA2 protein, S cerevisiae
DAPI
Oxidoreductases
Oxidoreductases Acting on CH-CH Group Donors
Aspartate Carbamoyltransferase
Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)