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Bait Protein: VAM3
Organism: Saccharomyces cerevisiae
Publication: Hao Xu , Youngsoo Jun, James Thompson, John Yates and William Wickner (2010) HOPS Prevents the Disassembly of Trans-SNARE Complexes by Sec17p/Sec18p during Membrane Fusion [In Press]
Comments: Sample: HX11 - both fusion and trans-SNARE complex formation take place.

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   SEC17 Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' ste... 24 247 66.8% 0.0635 32803
[View Peptides]   VAM7 Component of the vacuole SNARE complex involved in vacuolar morphogenesis; SNAP-25 homolog; function... 19 247 49.1% 0.0587 36711
[View Peptides]   NYV1 v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent C... 10 55 39.9% 0.0163 28964
[View Peptides]   VAM3 Syntaxin-related protein required for vacuolar assembly; functions with Vam7p in vacuolar protein tr... 14 189 35.3% 0.0501 32498
[View Peptides]   RPP2B Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... 2 3 28.2% 0.00205 11050
[View Peptides]   PDA1 E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarbo... 12 46 27.6% 0.00822 46343
[View Peptides]   VTI1 Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and ... 6 175 26.7% 0.0605 24668
[View Peptides]   RPP2A Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... 2 2 23.6% 0.00142 10746
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 7 16 22.0% 0.0024 54545
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 12 77 21.1% 0.00847 74468
[View Peptides]   LAT1 Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes... 9 48 19.5% 0.00747 51818
[View Peptides]   PHO8 Repressible alkaline phosphatase, a glycoprotein localized to the vacuole; regulated by levels of in... 10 39 18.9% 0.00517 63004
[View Peptides]   PDB1 E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved mu... 5 60 18.9% 0.0123 40054
[View Peptides]   SEC4 Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with t... 3 7 17.2% 0.00244 23506
[View Peptides]   IVY1 Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the... 5 6 16.8% 9.94E-4 49976
[View Peptides]   SHM2 Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrof... 8 18 16.6% 0.00288 52219
[View Peptides]   VAM6 Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by fa... 13 44 16.2% 0.00315 122881
[View Peptides]   PSA1 GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from ... 4 12 15.8% 0.00249 39566
[View Peptides]   SSC1 Hsp70 family ATPase, constituent of the import motor component of the Translocase of the Inner Mitoc... 6 8 15.7% 9.18E-4 70628
[View Peptides]   VMA6 Subunit d of the five-subunit V0 integral membrane domain of vacuolar H+-ATPase (V-ATPase), an elect... 3 4 14.8% 8.7E-4 39791
[View Peptides]   PMA1 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... 7 13 14.1% 0.00106 99619
[View Peptides]   ADE5,7 Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole... 7 10 13.8% 9.36E-4 86068
[View Peptides]   PPN1 Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer 6 12 13.6% 0.00134 78344
[View Peptides]   YDR089W Protein of unknown function; deletion confers resistance to Nickel 10 43 13.5% 0.00371 98712
[View Peptides]   PDX1 Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogena... 4 5 13.4% 9.15E-4 45362
[View Peptides]   VPH1 Subunit a of vacuolar-ATPase V0 domain, one of two isoforms (Vph1p and Stv1p); Vph1p is located in V... 8 28 13.1% 0.0025 95529
[View Peptides]   CPS1 Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions 6 11 12.5% 0.00143 64597
[View Peptides]   TSA1 Thioredoxin peroxidase, acts as both a ribosome-associated and free cytoplasmic antioxidant; self-as... 2 2 12.2% 7.66E-4 21590
[View Peptides]   YKT6 Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and wit... 2 9 12.0% 0.00338 22707
[View Peptides]   YPK9 Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-t... 12 41 11.9% 0.00209 166749
[View Peptides]   DBP2 Essential ATP-dependent RNA helicase of the DEAD-box protein family, involved in nonsense-mediated m... 5 10 11.4% 0.00137 60999
[View Peptides]   YNL058C Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to th... 2 8 11.4% 0.0019 35046
[View Peptides]   SEC18 ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuol... 5 10 11.2% 9.9E-4 84056
[View Peptides]   CCC1 Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack ... 3 3 11.2% 6.99E-4 34250
[View Peptides]   VTC3 Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuol... 7 21 11.0% 0.00189 96553
[View Peptides]   VTC4 Vacuolar membrane polyphosphate polymerase; subunit of the vacuolar transporter chaperone (VTC) comp... 6 17 10.8% 0.00177 83155
[View Peptides]   VPS16 Subunit of the vacuole fusion and protein sorting HOPS complex and the CORVET tethering complex; par... 7 7 10.5% 6.58E-4 92741
[View Peptides]   NOP56 Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that ... 3 6 10.5% 8.93E-4 56864
[View Peptides]   PDI1 Protein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, es... 3 9 10.2% 0.00129 58227
[View Peptides]   PHO84 High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated... 4 27 10.1% 0.00345 64382
[View Peptides]   DAP2 Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membra... 5 20 9.5% 0.00183 93404
[View Peptides]   VAC8 Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for ... 4 14 9.2% 0.00182 63208
[View Peptides]   VMA2 Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... 3 3 8.9% 4.35E-4 57749
[View Peptides]   RPP0 Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphoryl... 2 8 8.7% 0.00192 33717
[View Peptides]   TFP1 Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein prec... 9 24 8.6% 0.00168 118637
[View Peptides]   HMG1 One of two isozymes of HMG-CoA reductase that catalyzes the conversion of HMG-CoA to mevalonate, whi... 6 22 8.4% 0.00157 115625
[View Peptides]   LPD1 Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydroge... 3 7 8.4% 0.00105 54010
[View Peptides]   VPS41 Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sort... 7 21 8.3% 0.00159 113412
[View Peptides]   SEC63 Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/Bi... 4 5 8.3% 5.66E-4 75345
[View Peptides]   YNL217W Hypothetical protein 2 4 8.3% 9.21E-4 37151
[View Peptides]   SED5 cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, bi... 2 2 8.2% 4.41E-4 38807
[View Peptides]   TUB1 Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form... 2 3 8.1% 5.04E-4 49800
[View Peptides]   FKS1 Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... 9 22 8.0% 8.8E-4 214849
[View Peptides]   YCF1 Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detox... 9 31 7.9% 0.00154 171120
[View Peptides]   YME1 Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible f... 5 11 7.9% 0.00111 81772
[View Peptides]   LCB4 Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which fun... 3 4 7.9% 4.81E-4 69639
[View Peptides]   DED1 ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... 3 3 7.8% 3.73E-4 65553
[View Peptides]   PGK1 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phospha... 2 3 7.7% 5.41E-4 44738
[View Peptides]   FRA1 Protein involved in negative regulation of transcription of iron regulon; forms an iron independent ... 4 20 7.2% 0.002 84924
[View Peptides]   YDL180W Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to th... 2 3 7.1% 4.12E-4 62288
[View Peptides]   VAC7 Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p k... 8 12 7.0% 7.73E-4 128140
[View Peptides]   KTR1 Alpha-1,2-mannosyltransferase involved in O- and N-linked protein glycosylation; type II membrane pr... 2 4 6.9% 7.64E-4 46022
[View Peptides]   RMD8 Cytosolic protein required for sporulation 3 5 6.8% 5.67E-4 75914
[View Peptides]   SAC1 Phosphatidylinositol phosphate (PtdInsP) phosphatase involved in hydrolysis of PtdIns[4]P; transmemb... 3 5 6.7% 6.02E-4 71125
[View Peptides]   KTR6 Probable mannosylphosphate transferase involved in the synthesis of core oligosaccharides in protein... 2 2 6.5% 3.37E-4 52128
[View Peptides]   PEP3 Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular ... 5 6 6.3% 4.9E-4 107398
[View Peptides]   PEP5 Component of CORVET tethering complex; peripheral vacuolar membrane protein required for protein tra... 6 16 6.2% 0.00117 117477
[View Peptides]   MNN1 Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for add... 4 9 6.0% 8.86E-4 88529
[View Peptides]   YCK3 Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fus... 2 2 5.9% 2.86E-4 60261
[View Peptides]   TUB2 Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymeriz... 2 2 5.9% 3.28E-4 50923
[View Peptides]   MNR2 Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has si... 4 8 5.8% 6.2E-4 109736
[View Peptides]   URA2 Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes t... 9 14 5.7% 4.75E-4 245123
[View Peptides]   CHS1 Chitin synthase I, requires activation from zymogenic form in order to catalyze the transfer of N-ac... 4 6 5.7% 3.98E-4 129919
[View Peptides]   TDA7 Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p 2 3 5.7% 3.54E-4 67357
[View Peptides]   PCL8 Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen s... 2 3 5.5% 4.58E-4 55431
[View Peptides]   YBT1 Transporter of the ATP-binding cassette (ABC) family involved in bile acid transport; similar to mam... 7 14 5.4% 6.33E-4 189161
[View Peptides]   STD1 Protein involved in control of glucose-regulated gene expression; interacts with protein kinase Snf1... 2 2 5.4% 3.38E-4 50261
[View Peptides]   MPS3 Nuclear envelope protein required for SPB duplication and nuclear fusion; localizes to the SPB half ... 2 2 5.1% 2.2E-4 79175
[View Peptides]   PHO90 Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthet... 3 7 5.0% 5.96E-4 97689
[View Peptides]   HSP60 Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides... 2 2 4.9% 2.62E-4 60752
[View Peptides]   SKG6 Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell ... 2 2 4.8% 2.04E-4 81849
[View Peptides]   VTC2 Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuol... 3 3 4.7% 2.72E-4 95441
[View Peptides]   FAA1 Long chain fatty acyl-CoA synthetase, activates imported fatty acids with a preference for C12:0-C16... 2 2 4.7% 2.14E-4 77866
[View Peptides]   MAM3 Protein required for normal mitochondrial morphology, has similarity to hemolysins 3 3 4.5% 3.19E-4 77712
[View Peptides]   TAF7 TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation 2 2 4.4% 2.54E-4 67556
[View Peptides]   PYK2 Pyruvate kinase that appears to be modulated by phosphorylation; PYK2 transcription is repressed by ... 2 2 4.2% 2.97E-4 55195
[View Peptides]   DBP1 Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability o... 2 2 4.1% 2.43E-4 67917
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 2 7 4.1% 0.00115 50033
[View Peptides]   YER138C Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 5 9 3.9% 3.85E-4 198561
[View Peptides]   CHS2 Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of ... 2 2 3.8% 1.56E-4 109882
[View Peptides]   SYG1 Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G... 2 2 3.5% 1.66E-4 104217
[View Peptides]   VBA4 Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GF... 2 2 3.4% 1.95E-4 85683
[View Peptides]   KRE6 Protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; appears functional... 2 2 3.3% 2.08E-4 80123
[View Peptides]   PHO91 Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90,... 4 11 3.2% 9.23E-4 99490
[View Peptides]   CYK3 SH3-domain protein located in the mother-bud neck and the cytokinetic actin ring; mutant phenotype a... 2 2 3.1% 1.7E-4 100621
[View Peptides]   VPS33 ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essen... 2 2 3.0% 2.17E-4 79271
[View Peptides]   HMG2 One of two isozymes of HMG-CoA reductase that convert HMG-CoA to mevalonate, a rate-limiting step in... 2 3 2.8% 2.15E-4 115692
[View Peptides]   YLR241W Putative protein of unknown function, may be involved in detoxification 2 6 2.8% 5.76E-4 89344
[View Peptides]   PEP1 Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golg... 3 3 2.7% 1.43E-4 177777
[View Peptides]   ENA1 P-type ATPase sodium pump, involved in Na+ and Li+ efflux to allow salt tolerance 2 2 2.7% 1.38E-4 120357
[View Peptides]   ENA2 P-type ATPase sodium pump, involved in Na+ efflux to allow salt tolerance; likely not involved in Li... 2 2 2.7% 1.38E-4 120317
[View Peptides]   ENA5 Protein with similarity to P-type ATPase sodium pumps, member of the Na+ efflux ATPase family 2 2 2.7% 1.38E-4 120296
[View Peptides]   PDR5 Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter actively regulated by ... 2 2 2.4% 9.93E-5 170437
[View Peptides]   YGR125W Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal ... 2 2 2.4% 1.45E-4 116971
[View Peptides]   MIS1 Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation ... 2 2 2.4% 1.54E-4 106217
[View Peptides]   PMC1 Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activ... 2 2 2.2% 1.28E-4 130861
[View Peptides]   RTC1 Protein of unknown function; may interact with ribosomes, based on co-purification experiments; null... 2 2 2.1% 1.12E-4 149269
[View Peptides]   MHP1 Microtubule-associated protein involved in assembly and stabilization of microtubules; overproductio... 2 4 2.0% 2.15E-4 155207
[View Peptides]   SNF2 Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation... 2 4 1.9% 1.76E-4 194050
[View Peptides]   BPT1 ABC type transmembrane transporter of MRP/CFTR family, found in vacuolar membrane, involved in the t... 2 7 1.9% 3.37E-4 176873
[View Peptides]   RTG2 Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcri... 2 3 1.5% 3.83E-4 65572
[View Peptides]   GCN1 Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn20p; proposed to stimulate ... 2 2 0.8% 5.62E-5 296697

YRC Informatics Platform - Version 3.0
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