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Bait Protein: DAD1
Organism: Saccharomyces cerevisiae
Publication: Shimogawa M. M. et al. (2006) Mps1 phosphorylation of Dam1 couples kinetochores to microtubule plus ends at metaphase. Curr Biol. 2006 Aug 8;16(15):1489-501.
Comments: Dam1-765 complex purified from yeast with Dad1-TAP

Raw Data:

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FILTERED Run Results: [unfilter]

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   DAD1 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 9 19 97.9% 0.028 10516
[View Peptides]   ASK1 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 25 68 84.6% 0.0322 32056
[View Peptides]   SPC19 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 23 51 82.4% 0.0428 18910
[View Peptides]   DAD3 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 10 20 77.7% 0.0295 10848
[View Peptides]   DUO1 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 30 74 77.3% 0.0415 27473
[View Peptides]   SPC34 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 25 67 69.8% 0.0314 34078
[View Peptides]   HSK3 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 4 4 66.7% 0.00802 8088
[View Peptides]   ASC1 G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 th... 14 24 64.3% 0.0104 34805
[View Peptides]   DAM1 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 23 52 54.5% 0.021 38422
[View Peptides]   PSA1 GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from ... 17 30 48.8% 0.0115 39566
[View Peptides]   ACT1 Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... 9 17 39.7% 0.00628 41690
[View Peptides]   ADH1 Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the red... 13 25 38.2% 0.00994 36849
[View Peptides]   DAD4 Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced... 4 18 36.1% 0.0346 8155
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 15 25 33.2% 0.00756 50033
[View Peptides]   VMA2 Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... 10 13 31.1% 0.00348 57749
[View Peptides]   MIR1 Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redunda... 4 5 30.2% 0.00223 32812
[View Peptides]   ERG13 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-Co... 8 11 26.9% 0.0031 55014
[View Peptides]   YEF3 Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes b... 12 15 26.1% 0.00199 115945
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 7 11 25.0% 0.00305 54545
[View Peptides]   GFA1 Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glut... 11 11 24.5% 0.00212 80047
[View Peptides]   ATP3 Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily ... 5 5 23.5% 0.00223 34351
[View Peptides]   YHB1 Nitric oxide oxidoreductase, flavohemoglobin involved in nitric oxide detoxification; plays a role i... 4 4 22.8% 0.00139 44646
[View Peptides]   ILV2 Acetolactate synthase, catalyses the first common step in isoleucine and valine biosynthesis and is ... 6 6 21.5% 0.00121 74937
[View Peptides]   ATP2 Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily c... 5 6 19.8% 0.00163 54794
[View Peptides]   APA1 Diadenosine 5',5''-P1,P4-tetraphosphate phosphorylase I (AP4A phosphorylase), involved in catabolism... 2 3 19.3% 0.00129 36493
[View Peptides]   TFP1 Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein prec... 11 14 17.2% 0.00181 118637
[View Peptides]   CCT4 Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of... 4 5 17.2% 0.00131 57604
[View Peptides]   ARF1 ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated vesicle form... 3 3 17.1% 0.00229 20529
[View Peptides]   EFT2, EFT1 Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... 8 11 16.3% 0.00181 93289
[View Peptides]   PDC1 Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... 4 5 15.5% 0.00123 61495
[View Peptides]   TUB2 Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymeriz... 3 8 15.1% 0.00242 50923
[View Peptides]   DED1 ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... 4 5 14.2% 0.00115 65553
[View Peptides]   GRS1 Cytoplasmic and mitochondrial glycyl-tRNA synthase that ligates glycine to the cognate anticodon bea... 6 8 14.1% 0.00166 75411
[View Peptides]   KAP123 Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and... 7 9 13.5% 0.00112 122601
[View Peptides]   YHR020W Protein of unknown function that may interact with ribosomes, based on co-purification experiments; ... 6 8 13.2% 0.00161 77386
[View Peptides]   ADE5,7 Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole... 5 7 13.1% 0.00121 86068
[View Peptides]   PET9 Major ADP/ATP carrier of the mitochondrial inner membrane, exchanges cytosolic ADP for mitochondrial... 2 3 12.9% 0.00131 34426
[View Peptides]   GUS1 Glutamyl-tRNA synthetase (GluRS), forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; ... 6 8 12.3% 0.00156 80843
[View Peptides]   DPM1 Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P... 2 2 12.0% 0.00104 30362
[View Peptides]   ENO2 Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... 2 5 11.9% 0.00158 46914
[View Peptides]   TUB1 Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form... 3 5 11.6% 0.00155 49800
[View Peptides]   RPP0 Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphoryl... 2 2 11.2% 8.87E-4 33717
[View Peptides]   RPT4 One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation ... 3 3 10.3% 9.5E-4 49408
[View Peptides]   ILV1 Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under genera... 3 4 10.1% 9.61E-4 63831
[View Peptides]   ATP1 Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily ... 3 5 9.7% 0.00127 58618
[View Peptides]   SPC42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... 2 2 9.1% 7.63E-4 42271
[View Peptides]   URA2 Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes t... 10 10 8.8% 6.25E-4 245123
[View Peptides]   THS1 Threonyl-tRNA synthetase, essential cytoplasmic protein 4 5 8.3% 9.43E-4 84520
[View Peptides]   PMA1 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... 3 4 7.7% 6.03E-4 99619
[View Peptides]   NDE1 Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes t... 2 2 7.7% 4.94E-4 62774
[View Peptides]   CYS4 Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the ... 2 2 7.7% 5.46E-4 56022
[View Peptides]   RPT6 One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation ... 2 2 7.7% 6.84E-4 45272
[View Peptides]   PMA2 Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator ... 3 4 7.5% 5.85E-4 102172
[View Peptides]   NOP58 Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the ... 2 3 6.8% 8.13E-4 56956
[View Peptides]   SES1 Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), di... 2 3 6.7% 8.99E-4 53310
[View Peptides]   URA7 Major CTP synthase isozyme (see also URA8), catalyzes the ATP-dependent transfer of the amide nitrog... 2 2 6.0% 4.78E-4 64710
[View Peptides]   HIS4 Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrol... 3 4 5.8% 6.93E-4 87721
[View Peptides]   ALA1 Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation... 3 3 5.3% 4.33E-4 107277
[View Peptides]   FAA1 Long chain fatty acyl-CoA synthetase, activates imported fatty acids with a preference for C12:0-C16... 2 2 5.0% 3.95E-4 77866
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 2 2 4.7% 4.06E-4 74468
[View Peptides]   CCT8 Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of... 2 2 4.4% 4.87E-4 61662
[View Peptides]   PFK1 Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaer... 2 2 4.1% 2.8E-4 107970
[View Peptides]   MES1 Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which in... 2 2 3.9% 3.69E-4 85678
[View Peptides]   MET5 Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by meth... 2 2 3.7% 1.92E-4 161218
[View Peptides]   ACC1 Acetyl-CoA carboxylase, biotin containing enzyme that catalyzes the carboxylation of acetyl-CoA to f... 4 4 3.1% 2.48E-4 250351
[View Peptides]   PYC2 Pyruvate carboxylase isoform, cytoplasmic enzyme that converts pyruvate to oxaloacetate; highly simi... 3 3 3.1% 3.52E-4 130167
[View Peptides]   PYC1 Pyruvate carboxylase isoform, cytoplasmic enzyme that converts pyruvate to oxaloacetate; highly simi... 3 3 3.1% 3.53E-4 130099
[View Peptides]   DUR1,2 Urea amidolyase, contains both urea carboxylase and allophanate hydrolase activities, degrades urea ... 2 2 2.8% 1.51E-4 201830
[View Peptides]   YDR261C-D Retrotransposon TYA Gag and TYB Pol genes; in YDRCTY1-3 TYB is mutant and probably non-functional 2 2 1.9% 1.73E-4 181660
[View Peptides]   YGR027W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 1.8% 1.58E-4 198656
[View Peptides]   YBR012W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 1.8% 1.58E-4 198868
[View Peptides]   YDR098C-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 1.8% 1.58E-4 198594
[View Peptides]   YGR038C-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 1.8% 1.58E-4 198520
[View Peptides]   YDR365W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 1.8% 1.58E-4 198658
[View Peptides]   YER160C Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 1.8% 1.58E-4 198568
[View Peptides]   YDR316W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 1.8% 1.58E-4 198526
[View Peptides]   YER138C Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 1.8% 1.58E-4 198561
[View Peptides]   YDR210C-D Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 1.8% 1.58E-4 198838

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