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Bait Protein: SEC10
Organism: Saccharomyces cerevisiae
Publication: De Craene, J., Coleman, J., de Martin, P.E., Pypaert, M., Anderson, S., Yates, J.R. 3rd, Ferro-Novick, S., and Novick, P. (2006). Rtn1p is involved in structuring the cortical ER. Mol Biol Cell. In Press
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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   EXO70 Subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), whi... 20 33 35.8% 0.0107 71294
[View Peptides]   CLC1 Clathrin light chain, subunit of the major coat protein involved in intracellular protein transport ... 6 12 32.2% 0.0104 26532
[View Peptides]   TIM13 Mitochondrial intermembrane space protein, forms a complex with Tim8p that delivers a subset of hydr... 2 2 31.4% 0.00384 11286
[View Peptides]   EXO84 Essential protein with dual roles in spliceosome assembly and exocytosis; the exocyst complex (Sec3p... 22 33 30.9% 0.00884 85538
[View Peptides]   TIM11 Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex re... 2 3 30.2% 0.0063 10875
[View Peptides]   TIM44 Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers ... 10 12 29.0% 0.00562 48854
[View Peptides]   ATP7 Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily c... 6 6 27.6% 0.00696 19810
[View Peptides]   SEC5 Essential 107kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p,... 21 31 25.8% 0.00644 112121
[View Peptides]   RPP2A Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... 3 3 23.6% 0.00571 10746
[View Peptides]   SEC3 Subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, Exo84p) which me... 26 38 22.5% 0.00574 154694
[View Peptides]   SEC10 Essential 100kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p,... 18 23 21.5% 0.00533 100342
[View Peptides]   SEC8 Essential 121kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p,... 17 26 21.5% 0.00492 122224
[View Peptides]   SEC15 Essential 113kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p,... 20 28 18.8% 0.00621 105064
[View Peptides]   MRPL13 Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation 4 5 18.5% 0.00367 31526
[View Peptides]   SEC6 Essential 88kDa subunit of the exocyst complex, which mediates polarized targeting of secretory vesi... 14 20 17.3% 0.00501 93428
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 5 5 16.8% 0.00202 54545
[View Peptides]   CYT1 Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme... 3 3 16.5% 0.00196 34055
[View Peptides]   PSA1 GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from ... 4 4 15.8% 0.00224 39566
[View Peptides]   PDB1 E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved mu... 4 6 15.6% 0.00331 40054
[View Peptides]   RTT103 Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription terminatio... 4 4 15.6% 0.00197 46488
[View Peptides]   LAT1 Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes... 5 5 15.4% 0.00209 51818
[View Peptides]   KRE2 Alpha1,2-mannosyltransferase of the Golgi involved in protein mannosylation 4 6 15.2% 0.00274 51387
[View Peptides]   SRV2 CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cycl... 4 5 14.6% 0.00192 57522
[View Peptides]   KTR6 Probable mannosylphosphate transferase involved in the synthesis of core oligosaccharides in protein... 3 5 13.9% 0.00226 52128
[View Peptides]   NUP60 Subunit of the nuclear pore complex (NPC), functions to anchor Nup2p to the NPC in a process control... 4 5 13.7% 0.00187 59039
[View Peptides]   PIL1 Primary component of eisosomes, which are large immobile cell cortex structures associated with endo... 3 3 13.3% 0.00179 38349
[View Peptides]   RTN1 ER membrane protein that interacts with Sey1p to maintain ER morphology; interacts with exocyst subu... 3 3 13.2% 0.00205 32916
[View Peptides]   SSE1 ATPase that is a component of the heat shock protein Hsp90 chaperone complex; binds unfolded protein... 6 8 12.1% 0.00233 77367
[View Peptides]   VMA4 Subunit E of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... 3 3 11.6% 0.0026 26471
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 6 6 11.6% 0.00264 50033
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 6 6 11.6% 0.00264 50033
[View Peptides]   YGL082W Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; g... 2 3 10.5% 0.00159 43321
[View Peptides]   IMD3 Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-... 3 4 10.5% 0.00154 56585
[View Peptides]   LSP1 Primary component of eisosomes, which are large immobile patch structures at the cell cortex associa... 2 2 10.3% 0.00118 38071
[View Peptides]   GDA1 Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golg... 3 4 10.2% 0.00156 56822
[View Peptides]   YBR139W Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent prot... 4 4 9.1% 0.00159 57639
[View Peptides]   ACT1 Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... 2 3 9.1% 0.00161 41690
[View Peptides]   MCR1 Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis 2 2 8.9% 0.00134 34138
[View Peptides]   NCP1 NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately reg... 4 5 8.5% 0.00146 76772
[View Peptides]   GUS1 Glutamyl-tRNA synthetase (GluRS), forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; ... 6 6 8.4% 0.00167 82663
[View Peptides]   MNN5 Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of th... 3 3 7.5% 0.00103 67252
[View Peptides]   ARC1 Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering t... 2 3 7.2% 0.00161 42084
[View Peptides]   DJP1 Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in perox... 2 2 7.2% 9.34E-4 48575
[View Peptides]   RVS167 Actin-associated protein, interacts with Rvs161p to regulate actin cytoskeleton, endocytosis, and vi... 2 2 6.8% 8.37E-4 52774
[View Peptides]   PIB2 Protein binding phosphatidylinositol 3-phosphate, involved in telomere-proximal repression of gene e... 2 2 6.5% 6.35E-4 70617
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 3 4 6.2% 0.00118 74468
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 3 4 6.2% 0.00118 74468
[View Peptides]   PDA1 E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarbo... 2 2 5.6% 9.11E-4 48891
[View Peptides]   SSC1 Hsp70 family ATPase, constituent of the import motor component of the Translocase of the Inner Mitoc... 2 3 5.5% 9.25E-4 70628
[View Peptides]   DED1 ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... 2 2 5.3% 6.68E-4 65553
[View Peptides]   HSP60 Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides... 2 2 5.1% 7.05E-4 60752
[View Peptides]   HSC82 Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... 2 2 4.4% 5.72E-4 80900
[View Peptides]   GLT1 NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglut... 2 3 1.9% 2.82E-4 238100

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