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Bait Protein: RLF2
Organism: Saccharomyces cerevisiae
Publication: Green EM, Antczak AJ, Bailey AO, Franco AA, Wu KJ, Yates JR 3rd, Kaufman PD. Replication-independent histone deposition by the HIR complex and Asf1. Curr Biol. 2005 Nov 22;15(22):2044-9.
Comments: identification data - first search in cascade

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   MSI1 Subunit of chromatin assembly factor I (CAF-1), negative regulator of the RAS/cAMP pathway via seque... 53 493 79.6% 0.0906 47365
[View Peptides]   CAC2 Component of the chromatin assembly complex (with Rlf2p and Msi1p) that assembles newly synthesized ... 39 332 62.6% 0.055 51252
[View Peptides]   RLF2 Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1) with Cac2p and Msi1p that assembles ... 62 398 59.1% 0.0509 70210
[View Peptides]   RPP1A Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation facto... 3 10 49.1% 0.00732 10908
[View Peptides]   HTA2 Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of tw... 6 18 39.4% 0.0106 13989
[View Peptides]   HTA1 Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of tw... 6 18 39.4% 0.0106 13989
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 17 97 38.6% 0.0164 50033
[View Peptides]   RPP2B Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... 2 6 32.7% 0.00423 11050
[View Peptides]   RPP2A Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... 2 4 31.1% 0.00293 10746
[View Peptides]   HHT1, HHT2 Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated t... 6 23 28.7% 0.0131 15356
[View Peptides]   HHF1, HHF2 Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two... 3 14 28.2% 0.0105 11368
[View Peptides]   RNQ1 [PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate 16 48 26.9% 0.00919 42580
[View Peptides]   HTB2 Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly id... 3 12 26.0% 0.00711 14237
[View Peptides]   HTB1 Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly id... 3 12 26.0% 0.00711 14252
[View Peptides]   RPP0 Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphoryl... 6 16 22.8% 0.00398 33717
[View Peptides]   SPC42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... 4 4 20.7% 8.55E-4 42271
[View Peptides]   RSC58 Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and e... 5 9 19.5% 0.00139 57795
[View Peptides]   ARF1 ADP-ribosylation factor, GTPase of the Ras superfamily involved in regulation of coated vesicle form... 2 2 19.3% 8.57E-4 20529
[View Peptides]   YNL208W Protein of unknown function; may interact with ribosomes, based on co-purification experiments; auth... 2 2 16.7% 7.6E-4 21964
[View Peptides]   TFS1 Protein that interacts with and inhibits carboxypeptidase Y and Ira2p; phosphatidylethanolamine-bind... 2 2 15.1% 7.08E-4 24357
[View Peptides]   DED1 ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... 5 15 14.9% 0.00193 65553
[View Peptides]   STP22 Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into ... 2 2 14.5% 5.24E-4 33304
[View Peptides]   YHM2 Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing n... 2 4 13.4% 9.88E-4 34185
[View Peptides]   ADH1 Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the red... 2 2 13.2% 4.46E-4 36849
[View Peptides]   ARP7 Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved... 3 3 13.0% 4.88E-4 53810
[View Peptides]   RVB1 Essential protein involved in transcription regulation; component of chromatin remodeling complexes;... 3 5 12.1% 8.38E-4 50453
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 3 4 11.8% 6.21E-4 54545
[View Peptides]   NOP56 Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that ... 3 4 11.3% 6.16E-4 56864
[View Peptides]   RSC8 Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homol... 4 9 11.1% 0.00125 63168
[View Peptides]   NUG1 GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits... 4 4 10.8% 5.97E-4 57709
[View Peptides]   NPL6 Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Ldb7p, and Htl1p to... 2 5 10.8% 8.92E-4 49651
[View Peptides]   PET9 Major ADP/ATP carrier of the mitochondrial inner membrane, exchanges cytosolic ADP for mitochondrial... 2 5 10.4% 0.00122 34426
[View Peptides]   SUM1 Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also... 6 8 10.3% 5.84E-4 118201
[View Peptides]   NCP1 NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately reg... 4 11 10.0% 0.00123 76772
[View Peptides]   PMA1 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... 5 10 9.9% 8.45E-4 99619
[View Peptides]   DEF1 RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and prot... 5 9 9.9% 9.46E-4 83973
[View Peptides]   FUN12 GTPase, required for general translation initiation by promoting Met-tRNAiMet binding to ribosomes a... 5 10 9.6% 7.74E-4 112268
[View Peptides]   SVP26 Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II... 3 3 9.6% 0.00102 26284
[View Peptides]   VMA2 Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... 3 3 9.1% 4.5E-4 57749
[View Peptides]   STH1 ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic g... 7 12 7.9% 6.85E-4 156742
[View Peptides]   RIM20 Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix fam... 2 2 7.6% 2.35E-4 75947
[View Peptides]   PMA2 Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator ... 4 6 7.3% 4.91E-4 102172
[View Peptides]   RVB2 Essential protein involved in transcription regulation; component of chromatin remodeling complexes;... 2 2 7.2% 3.29E-4 51612
[View Peptides]   YHL008C Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appea... 2 5 6.9% 6.19E-4 69969
[View Peptides]   FKS1 Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... 6 6 6.4% 2.48E-4 214849
[View Peptides]   SSZ1 Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that ... 3 4 5.9% 5.77E-4 58238
[View Peptides]   RTT106 Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced ch... 2 3 5.9% 5.11E-4 51621
[View Peptides]   TOP2 Essential type II topoisomerase, relieves torsional strain in DNA by cleaving and re-sealing the pho... 5 6 5.7% 3.26E-4 164214
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 2 2 5.4% 2.27E-4 74468
[View Peptides]   HAL5 Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disrupt... 2 2 5.3% 1.81E-4 95455
[View Peptides]   YGR130C Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; possible compon... 3 3 4.7% 2.85E-4 92698
[View Peptides]   GCN20 Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate G... 2 2 4.4% 2.06E-4 85027
[View Peptides]   HIR2 Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene tra... 2 2 4.3% 1.77E-4 98445
[View Peptides]   SBE22 Protein involved in the transport of cell wall components from the Golgi to the cell surface; simila... 2 2 3.8% 1.82E-4 96398
[View Peptides]   PDR1 Zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins t... 2 3 3.7% 2.18E-4 121793
[View Peptides]   RGC1 Pleckstrin homology domain containing protein proposed to function as a glycerol channel activator; ... 2 2 3.7% 1.43E-4 120395
[View Peptides]   SPT5 Protein involved in regulating Pol I and Pol II transcription and pre-mRNA processing; forms a compl... 2 2 3.3% 1.46E-4 115650
[View Peptides]   GSC2 Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore... 3 3 3.2% 1.23E-4 216988
[View Peptides]   COG4 Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tet... 2 2 2.8% 1.8E-4 98665
[View Peptides]   YPL216W Putative protein of unknown function; YPL216W is not an essential gene 2 2 2.6% 1.41E-4 128133
[View Peptides]   SNF2 Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation... 2 2 2.5% 9.11E-5 194050
[View Peptides]   NUP159 Nucleoporin, subunit of the nuclear pore complex that is found exclusively on the cytoplasmic side, ... 2 2 2.5% 1.06E-4 158907
[View Peptides]   YJL113W Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 3 2.4% 1.29E-4 207708
[View Peptides]   YHL009W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 3 2.4% 1.29E-4 207969
[View Peptides]   KEM1 Evolutionarily-conserved 5'-3' exonuclease component of cytoplasmic processing (P) bodies involved i... 2 3 2.4% 1.52E-4 175459
[View Peptides]   RGA1 GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of se... 2 5 2.4% 3.85E-4 112831
[View Peptides]   SLH1 Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; req... 2 2 2.3% 7.89E-5 224828
[View Peptides]   OXP1 5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green... 2 2 2.3% 1.21E-4 140427
[View Peptides]   AUS1 Transporter of the ATP-binding cassette family, involved in uptake of sterols and anaerobic growth 2 2 2.0% 1.11E-4 157750
[View Peptides]   PDR5 Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter actively regulated by ... 2 2 1.9% 1.03E-4 170437
[View Peptides]   ACC1 Acetyl-CoA carboxylase, biotin containing enzyme that catalyzes the carboxylation of acetyl-CoA to f... 2 2 1.6% 6.95E-5 250351
[View Peptides]   ARO1 Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which i... 2 2 1.4% 9.77E-5 174754

YRC Informatics Platform - Version 3.0
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