Protein Overview: SEC28

General Information:
Name(s) found:	SEC28 / YIL076W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|82795256\|ref\|NP_012189.2\| Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; Sec28p [Saccharomyces cerevisiae] [NCBI NR] gi\|3915654\|sp\|P40509.2\|COPE_YEAST RecName: Full=Coatomer subunit epsilon; AltName: Full=Epsilon-coat protein; Short=Epsilon-COP [NCBI NR] gi\|2832291\|gb\|AAC14023.1\| epsilon-COP coatomer subunit Sec28p [Saccharomyces cerevisiae] [NCBI NR] Coatomer subunit epsilon OS=Saccharomyces cerevisiae GN=SEC28 PE=1 SV=2 [Swiss-Prot] SEC28 Epsilon-COP coatomer subunit [mips] Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth [SGD] (P40509) Coatomer epsilon subunit (Epsilon-coat protein) (Epsilon-COP) [4932.FASTACCERV.txt] Coatomer epsilon subunit (Epsilon-coat protein) (Epsilon-COP)gi\|2832291\|gb\|AAC14023.1\| epsilon-COP coatomer subunit Sec28p [Saccharomyces cerevisiae] [nrdb_11152004_Chlamydomonas] SEC28 SGDID:S000001338, Chr IX from 216655-217545, Verified ORF, "Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Coatomer subunit epsilon (Epsilon-coat protein) (Epsilon-COP) [nr-271106-contam.fasta] Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; Sec28p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] epsilon-COP coatomer subunit Sec28p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Coatomer subunit epsilon OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SEC28 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SEC28 SGDID:S000001338, Chr IX from 216658-217548, Genome Release 64-1-1, Verified ORF, "Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth" [yeast-030211-contam.fasta] SEC28 SGDID:S000001338, Chr IX from 216658-217548, Genome Release 64-2-1, Verified ORF, "Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress" [20141208_orf_trans.fasta] YIL076W SGDID:S000001338, Chr IX from 216658-217548, Genome Release 64-1-1, Verified ORF, "Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	296 amino acids
Gene Ontology:
Cellular Component:	endosome [IDA] COPI vesicle coat [IMP][ISS]
Biological Process:	vesicle coating [IGI][IMP] ER to Golgi vesicle-mediated transport [IMP]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDYFNIKQNY YTGNFVQCLQ EIEKFSKVTD NTLLFYKAKT LLALGQYQSQ DPTSKLGKVL  60
   61 DLYVQFLDTK NIEELENLLK DKQNSPYELY LLATAQAILG DLDKSLETCV EGIDNDEAEG 120
  121 TTELLLLAIE VALLNNNVST ASTIFDNYTN AIEDTVSGDN EMILNLAESY IKFATNKETA 180
  181 TSNFYYYEEL SQTFPTWKTQ LGLLNLHLQQ RNIAEAQGIV ELLLSDYYSV EQKENAVLYK 240
  241 PTFLANQITL ALMQGLDTED LTNQLVKLDH EHAFIKHHQE IDAKFDELVR KYDTSN

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		COP1 RET2 RET3 SEC21 SEC26 SEC27 SEC28
View Details	(MIPS) Mewes HW, et al. (2004)		ARF1 COP1 RET2 RET3 SEC21 SEC26 SEC27 SEC28
View Details	Krogan NJ, et al. (2006)		COP1 FCF2 FMP23 LCB5 PIP2 RET2 RET3 SCS2 SEC2 SEC21 SEC26 SEC27 SEC28
View Details	Gavin AC, et al. (2006)		COP1 RET2 RET3 SEC21 SEC26 SEC27 SEC28
View Details	Gavin AC, et al. (2002)		COP1 GLO3 GPT2 ILV1 PGK1 RET2 RET3 SAM1 SCJ1 SEC21 SEC26 SEC27 SEC28 SEH1 TOS1
View Details	Ho Y, et al. (2002)		ADR1 ARG4 ARP2 ATP3 AYR1 BTN2 CBK1 CCT2 CCT6 COP1 COR1 CPR6 CTR1 DHH1 EAP1 ERG27 FMP40 GAL7 GDT1 GPT2 IDH1 LIA1 MAE1 MEH1 MOB2 OM45 PCT1 PDA1 PET10 PET9 PHO86 PIL1 PRB1 PRE10 PRO3 RET2 RNR2 RPN7 RPT3 RTN2 RVS161 SEC18 SEC21 SEC26 SEC27 SEC28 SIS1 STI1 TAO3 TCP1 TIF34 TIF35 TPA1 TRP3 VMA6 YCR076C YNL181W
View Details	Qiu et al. (2008)		ARF1 ARF2 COP1 GLO3 RET2 SAR1 SEC16 SEC21 SEC26 SEC27 SEC28

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	SEC28	Huh WK, et al. (2003)
View Data	SEC28 and COP1	Huh WK, et al. (2003)
View Data	SEC28 and ANP1	Huh WK, et al. (2003)

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Protein Overview: SEC28

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: