Protein Overview: BNI1

General Information:
Name(s) found:	BNI1 / YNL271C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324058\|ref\|NP_014128.1\| Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1; Bni1 [NCBI NR] pir\|\|S63244 BNI1 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1705492\|sp\|P41832.2\|BNI1_YEAST RecName: Full=Protein BNI1; AltName: Full=Synthetic lethal 39; AltName: Full=Sensitive to high expression protein 5 [NCBI NR] gi\|1302334\|emb\|CAA96179.1\| BNI1 [Saccharomyces cerevisiae] [NCBI NR] Protein BNI1 OS=Saccharomyces cerevisiae GN=BNI1 PE=1 SV=2 [Swiss-Prot] BNI1 Regulator of budding [mips] Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 [SGD] Chr XIV from 129521-135382, reverse complement [yeastorf3.fasta] Chr XIV from 135382-129521, reverse complement [yeastorf5.fasta] (P41832) BNI1 protein (Synthetic lethal 39) [4932.FASTACCERV.txt] BNI1, Chr XIV from 129521-135382, reverse complement [yeast_orfs2] BNI1 SGDID:S0005215, Chr XIV from 135382-129521, reverse complement, Verified ORF [yeastflysequest.fasta] Bni1p [Saccharomyces cerevisiae]gi\|1302334\|emb\|CAA96179.1\| BNI1 [Saccharomyces cerevisiae]gi\|1705492\|sp\|P41832\|BNI1_YEAST BNI1 protein (Synthetic lethal 39)gi\|2131197\|pir\|\|S63244 BNI1 protein - yeast (Saccharomyces cerevisiae) [nrdb_11152004_Chlamydomonas] BNI1 SGDID:S000005215, Chr XIV from 135384-129523, reverse complement, Verified ORF, "Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of pola [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Protein BNI1 (Synthetic lethal 39) [nr-271106-contam.fasta] Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1; Bni1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] BNI1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] BNI1 SGDID:S000005215, Chr XIV from 135384-129523, reverse complement, Verified ORF, "Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1" [yeast-bovrabprots.fasta] [chemostatdb.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1953 amino acids
Gene Ontology:
Cellular Component:	polarisome [TAS] incipient cellular bud site [IDA] cellular bud tip [IDA] cellular bud neck [IDA] mating projection tip [IDA] actin filament [IDA] actin cap [IDA]
Biological Process:	intracellular mRNA localization [IMP] bipolar cellular bud site selection [TAS] regulation of initiation of mating projection growth [IMP] response to osmotic stress [TAS] pseudohyphal growth [IMP] actin filament organization [IPI] Rho protein signal transduction [IPI] budding cell bud growth [TAS] pheromone-dependent signal transduction involved in conjugation with cellular fusion [IMP] gene conversion at mating-type locus [IMP] establishment of mitotic spindle orientation [IMP] filamentous growth [IMP] actin nucleation [IDA] regulation of termination of mating projection growth [IMP]
Molecular Function:	cytoskeletal regulatory protein binding [IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLKNSGSKHS NSKESHSNSS SGIFQNLKRL ANSNATNSNT GSPTYASQQQ HSPVGNEVST  60
   61 SPASSSSFRK LNAPSRSTST EARPLNKKST LNTQNLSQYM NGKLSGDVPV SSQHARSHSM 120
  121 QSKYSYSKRN SSQASNKLTR QHTGQSHSAS SLLSQGSLTN LSKFTTPDGK IYLEMPSDPY 180
  181 EVEVLFEDIM YKRNIFQSLS EDKQEALMGY SIEKKWLIVK QDLQNELKKM RANTTSSSTA 240
  241 SRTSMASDHH PILTANSSLS SPKSVLMTSA SSPTSTVYSN SLNHSTTLSS VGTSTSKGKK 300
  301 LVSGSLKKQP SLNNIYRGGA ENNTSASTLP GDRTNRPPIH YVQRILADKL TSDEMKDLWV 360
  361 TLRTEQLDWV DAFIDHQGHI AMANVLMNSI YKTAPRENLT KELLEKENSF FKCFRVLSML 420
  421 SQGLYEFSTH RLMTDTVAEG LFSTKLATRK MATEIFVCML EKKNKSRFEA VLTSLDKKFR 480
  481 IGQNLHMIQN FKKMPQYFSH LTLESHLKII QAWLFAVEQT LDGRGKMGSL VGASDEFKNG 540
  541 GGENAILEYC QWTMVFINHL CSCSDNINQR MLLRTKLENC GILRIMNKIK LLDYDKVIDQ 600
  601 IELYDNNKLD DFNVKLEANN KAFNVDLHDP LSLLKNLWDI CKGTENEKLL VSLVQHLFLS 660
  661 SSKLIEENQN SSKLTKQLKL MDSLVTNVSV ASTSDEETNM NMAIQRLYDA MQTDEVARRA 720
  721 ILESRALTKK LEEIQAERDS LSEKLSKAEH GLVGQLEDEL HERDRILAKN QRVMQQLEAE 780
  781 LEELKKKHLL EKHQQEVELR KMLTILNSRP EESFNKNEGT RGMNSSLNSS EKANIQKVLQ 840
  841 DGLSRAKKDY KDDSKKFGMT LQPNKRLKML RMQMENIENE ARQLEMTNFA EFEKDRLEPP 900
  901 IHIKKPKVKK MKNKDRKPLV KPQEADVNKL NDLRRALTEI QMESNDISKF NVEERVNELF 960
  961 NEKKSLALKR LKELETKYKG FGIDFNVDEI MDSPKKNTGD VETEEDANYA SLDPKTYQKK1020
 1021 LDEINRITDQ LLDIQTQTEH EIQVEEDGES DLSSSSSDDE SEEIYQDASP TQELRSEHSE1080
 1081 LSSGSGPGSF LDALSQKYGT GQNVTASAAF GENNNGSGIG PLHSKVEKTF MNRLRKSTVS1140
 1141 SAPYLEELTQ KVNKVEPYEQ NEDEGLDKKS LPENSTASAA SAFDKAEKDM RQHVENGKQG1200
 1201 RVVNHEEDKT ADFSAVSKLN NTDGAEDLST QSSVLSSQPP PPPPPPPPVP AKLFGESLEK1260
 1261 EKKSEDDTVK QETTGDSPAP PPPPPPPPPP PMALFGKPKG ETPPPPPLPS VLSSSTDGVI1320
 1321 PPAPPMMPAS QIKSAVTSPL LPQSPSLFEK YPRPHKKLKQ LHWEKLDCTD NSIWGTGKAE1380
 1381 KFADDLYEKG VLADLEKAFA AREIKSLASK RKEDLQKITF LSRDISQQFG INLHMYSSLS1440
 1441 VADLVKKILN CDRDFLQTPS VVEFLSKSEI IEVSVNLARN YAPYSTDWEG VRNLEDAKPP1500
 1501 EKDPNDLQRA DQIYLQLMVN LESYWGSRMR ALTVVTSYER EYNELLAKLR KVDKAVSALQ1560
 1561 ESDNLRNVFN VILAVGNFMN DTSKQAQGFK LSTLQRLTFI KDTTNSMTFL NYVEKIVRLN1620
 1621 YPSFNDFLSE LEPVLDVVKV SIEQLVNDCK DFSQSIVNVE RSVEIGNLSD SSKFHPLDKV1680
 1681 LIKTLPVLPE ARKKGDLLED EVKLTIMEFE SLMHTYGEDS GDKFAKISFF KKFADFINEY1740
 1741 KKAQAQNLAA EEEERLYIKH KKIVEEQQKR AQEKEKQKEN SNSPSSEGNE EDEAEDRRAV1800
 1801 MDKLLEQLKN AGPAKSDPSS ARKRALVRKK YLSEKDNAPQ LLNDLDTEEG SILYSPEAMD1860
 1861 PTADTVIHAE SPTPLATRGV MNTSEDLPSP SKTSALEDQE EISDRARMLL KELRGSDTPV1920
 1921 KQNSILDEHL EKLRARKERS IGEASTGNRL SFK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Gavin AC, et al. (2002)		BNI1 CDC48 DOA1 FAL1 MRPS28 NPL4 PDC1 RAI1 REG1 RPA135 RPA190 RPO31 SHP1 UFD1 YDR049W
View Details	Ho Y, et al. (2002)		ADR1 ARC15 ARC18 ARC19 ARC35 ARC40 ARP2 ARP3 BNI1 DUR1,2 IMH1 KAP122 MCM4 MCM7 MET18 RPT4 TRP3 YJR029W
View Details	Qiu et al. (2008)		ABP1 ABP140 ACT1 AIP1 ARC40 ARP2 BBC1 BEM1 BIK1 BNI1 BNR1 BOI1 BOI2 BUD6 CAP1 CAP2 CDC24 COF1 CRN1 HOF1 HSK3 IQG1 MYO2 MYO4 PAN1 PEA2 PFY1 RVS167 SCP1 SHE3 SLA1 SLA2 SPA2 SPH1 SRV2 TPM2 TWF1 VRP1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	YNL260C	No Comments	Hazbun TR, et al. (2003)
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#22 Asynchronous Prep2-IMAC Phosphopeptide enrichment B1-2	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	ZDS2	BNI1	2	Drees BL, et al. (2001)
View Screen	BNI1	SYF1	2	Unpublished Fields Lab Data
View Screen	BNI1	XBP1	2	Unpublished Fields Lab Data
View Screen	BNI1	ADY4	2	Unpublished Fields Lab Data
View Screen	BNI1	YOR343C	2	Unpublished Fields Lab Data
View Screen	BNI1	NOC4	1	Unpublished Fields Lab Data
View Screen	HMT1	BNI1	1	Unpublished Fields Lab Data
View Screen	BNI1	YBR137W	1	Unpublished Fields Lab Data
View Screen	BNI1	YLR269C	1	Unpublished Fields Lab Data
View Screen	BNI1	SIN4	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	BNI1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..430]	PSI-BLAST	42.09691	Heavy meromyosin subfragment
2	View Details	[431..521]	PSI-BLAST	10.69897	Myosin S1 fragment, N-terminal domain; Myosin S1, motor domain
3	View Details	[522..814]	PSI-BLAST	8.0	Tropomyosin
4	View Details	[815..1263]	FFAS03	1.02	Structure of Unphosphorylated STAT1
5	View Details	[1264..1341]	deduced	N/A	No confident structure predictions are available.
6	View Details	[1342..1825]	PSI-BLAST	94.30103	Bni1p Formin Homology 2 Domain complexed with ATP-actin
7	View Details	[1826..1953]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2