YRC Public Data Repository - Protein Structure Prediction

Protein:	BNI1
Organism:	Saccharomyces cerevisiae
Length:	1953 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for BNI1.

Description	E-value	Query Range	Subject Range
BNI1 - Formin, nucleates the formation of linear actin filaments, involved in cell processes such as buddin... BNI1_YEAST - Protein BNI1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BNI1 PE=1 SV=3 gi\|190409240 - gi\|190409240\|gb\|EDV12505.1\| formin [Saccharomyces cerevisiae RM11-1a]	843.0	[0..1]	[1953..1]
gi\|68388302 - gi\|68388302\|ref\|XP_696268.1\| PREDICTED: similar to slow myosin heavy chain 1 [Danio rerio]	511.0	[0..410]	[1295..1733]
gi\|50757613 - gi\|50757613\|ref\|XP_415578.1\| PREDICTED: similar to myosin heavy chain [Gallus gallus]	400.0	[0..409]	[1295..689]
DAAM-PC - The gene Dishevelled Associated Activator of Morphogenesis is referred to in FlyBase by the symbol D...	396.0	[0..1165]	[1840..763]
gi\|114649940 - gi\|114649940\|ref\|XP_509808.2\| PREDICTED: diaphanous homolog 3 [Pan troglodytes]	386.0	[0..825]	[1832..176]
gi\|122889105, gi... - gi\|58532637\|gb\|AAW78862.1\| diaphanous-related formin 3 [Homo sapiens], gi\|123230067\|emb\|CAM19738.1\| ...	378.0	[0..825]	[1832..115]
gi\|50291265, gi\|... - gi\|50291265\|ref\|XP_448065.1\| unnamed protein product [Candida glabrata], gi\|49527376\|emb\|CAG61016.1\|... gi\|49527376 - gi\|49527376\|emb\|CAG61016.1\| unnamed protein product [Candida glabrata]	372.0	[0..670]	[1952..593]

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Predicted Domain #1

Region A:
Residues: [1-430]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLKNSGSKHS NSKESHSNSS SGIFQNLKRL ANSNATNSNT GSPTYASQQQ HSPVGNEVST  60
   61 SPASSSSFRK LNAPSRSTST EARPLNKKST LNTQNLSQYM NGKLSGDVPV SSQHARSHSM 120
  121 QSKYSYSKRN SSQASNKLTR QHTGQSHSAS SLLSQGSLTN LSKFTTPDGK IYLEMPSDPY 180
  181 EVEVLFEDIM YKRNIFQSLS EDKQEALMGY SIEKKWLIVK QDLQNELKKM RANTTSSSTA 240
  241 SRTSMASDHH PILTANSSLS SPKSVLMTSA SSPTSTVYSN SLNHSTTLSS VGTSTSKGKK 300
  301 LVSGSLKKQP SLNNIYRGGA ENNTSASTLP GDRTNRPPIH YVQRILADKL TSDEMKDLWV 360
  361 TLRTEQLDWV DAFIDHQGHI AMANVLMNSI YKTAPRENLT KELLEKENSF FKCFRVLSML 420
  421 SQGLYEFSTH

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	42.09691
Match:	1i84S
Description:	Heavy meromyosin subfragment
Matching Structure (courtesy of the PDB):

Predicted Domain #2

Region A:
Residues: [431-521]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 RLMTDTVAEG LFSTKLATRK MATEIFVCML EKKNKSRFEA VLTSLDKKFR IGQNLHMIQN  60
   61 FKKMPQYFSH LTLESHLKII QAWLFAVEQT L

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	10.69897
Match:	2mysA
Description:	Myosin S1 fragment, N-terminal domain; Myosin S1, motor domain
Matching Structure (courtesy of the PDB):

Predicted Domain #3

Region A:
Residues: [522-814]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 DGRGKMGSLV GASDEFKNGG GENAILEYCQ WTMVFINHLC SCSDNINQRM LLRTKLENCG  60
   61 ILRIMNKIKL LDYDKVIDQI ELYDNNKLDD FNVKLEANNK AFNVDLHDPL SLLKNLWDIC 120
  121 KGTENEKLLV SLVQHLFLSS SKLIEENQNS SKLTKQLKLM DSLVTNVSVA STSDEETNMN 180
  181 MAIQRLYDAM QTDEVARRAI LESRALTKKL EEIQAERDSL SEKLSKAEHG LVGQLEDELH 240
  241 ERDRILAKNQ RVMQQLEAEL EELKKKHLLE KHQQEVELRK MLTILNSRPE ESF

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	8.0
Match:	1c1gA
Description:	Tropomyosin
Matching Structure (courtesy of the PDB):

Predicted Domain #4

Region A:
Residues: [815-1263]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NKNEGTRGMN SSLNSSEKAN IQKVLQDGLS RAKKDYKDDS KKFGMTLQPN KRLKMLRMQM  60
   61 ENIENEARQL EMTNFAEFEK DRLEPPIHIK KPKVKKMKNK DRKPLVKPQE ADVNKLNDLR 120
  121 RALTEIQMES NDISKFNVEE RVNELFNEKK SLALKRLKEL ETKYKGFGID FNVDEIMDSP 180
  181 KKNTGDVETE EDANYASLDP KTYQKKLDEI NRITDQLLDI QTQTEHEIQV EEDGESDLSS 240
  241 SSSDDESEEI YQDASPTQEL RSEHSELSSG SGPGSFLDAL SQKYGTGQNV TASAAFGENN 300
  301 NGSGIGPLHS KVEKTFMNRL RKSTVSSAPY LEELTQKVNK VEPYEQNEDE GLDKKSLPEN 360
  361 STASAASAFD KAEKDMRQHV ENGKQGRVVN HEEDKTADFS AVSKLNNTDG AEDLSTQSSV 420
  421 LSSQPPPPPP PPPPVPAKLF GESLEKEKK

[Run NCBI BLAST on this sequence.]

Detection Method:	FFAS03
Confidence:	1.02
Match:	1yvlA
Description:	Structure of Unphosphorylated STAT1
Matching Structure (courtesy of the PDB):

Predicted Domain #5

Region A:
Residues: [1264-1341]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SEDDTVKQET TGDSPAPPPP PPPPPPPPMA LFGKPKGETP PPPPLPSVLS SSTDGVIPPA  60
   61 PPMMPASQIK SAVTSPLL

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #6

Region A:
Residues: [1342-1825]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 PQSPSLFEKY PRPHKKLKQL HWEKLDCTDN SIWGTGKAEK FADDLYEKGV LADLEKAFAA  60
   61 REIKSLASKR KEDLQKITFL SRDISQQFGI NLHMYSSLSV ADLVKKILNC DRDFLQTPSV 120
  121 VEFLSKSEII EVSVNLARNY APYSTDWEGV RNLEDAKPPE KDPNDLQRAD QIYLQLMVNL 180
  181 ESYWGSRMRA LTVVTSYERE YNELLAKLRK VDKAVSALQE SDNLRNVFNV ILAVGNFMND 240
  241 TSKQAQGFKL STLQRLTFIK DTTNSMTFLN YVEKIVRLNY PSFNDFLSEL EPVLDVVKVS 300
  301 IEQLVNDCKD FSQSIVNVER SVEIGNLSDS SKFHPLDKVL IKTLPVLPEA RKKGDLLEDE 360
  361 VKLTIMEFES LMHTYGEDSG DKFAKISFFK KFADFINEYK KAQAQNLAAE EEERLYIKHK 420
  421 KIVEEQQKRA QEKEKQKENS NSPSSEGNEE DEAEDRRAVM DKLLEQLKNA GPAKSDPSSA 480
  481 RKRA

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	94.30103
Match:	1y64B
Description:	Bni1p Formin Homology 2 Domain complexed with ATP-actin
Matching Structure (courtesy of the PDB):

Predicted Domain #7

Region A:
Residues: [1826-1953]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 LVRKKYLSEK DNAPQLLNDL DTEEGSILYS PEAMDPTADT VIHAESPTPL ATRGVMNTSE  60
   61 DLPSPSKTSA LEDQEEISDR ARMLLKELRG SDTPVKQNSI LDEHLEKLRA RKERSIGEAS 120
  121 TGNRLSFK

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits