Protein Overview: PRI2

General Information:
Name(s) found:	PRI2 / YKL045W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|666094\|emb\|CAA50626.1\| DNA primase large chain (P58) [Saccharomyces cerevisiae] [NCBI NR] gi\|6322806\|ref\|NP_012879.1\| Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair; Pri2p [Saccharomyces cerevisiae] [NCBI NR] gi\|486060\|emb\|CAA81880.1\| PRI2 [Saccharomyces cerevisiae] [NCBI NR] gi\|172235\|gb\|AAA34900.1\| PR12 [Saccharomyces cerevisiae] [NCBI NR] gi\|130899\|sp\|P20457.1\|PRI2_YEAST RecName: Full=DNA primase large subunit; AltName: Full=DNA primase 58 kDa subunit; AltName: Full=DNA polymerase alpha:primase complex p58 subunit; Short=Pol alpha-primase complex p58 subunit; Short=DNA polymerase-primase c [NCBI NR] pir\|\|A32497 DNA primase (EC 2.7.7.-) large chain - yeast (Saccharomyces cerevisiae) [NCBI NR] DNA primase large subunit OS=Saccharomyces cerevisiae GN=PRI2 PE=1 SV=1 [Swiss-Prot] PRI2 DNA-directed DNA polymerase alpha , 58 KD subunit (DNA primase) [mips] Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair [SGD] PRI2, Chr XI from 353134-354720 [yeast_orfs2] Chr XI from 353134-354720 [yeastorf3.fasta] (P20457) DNA primase large subunit (EC 2.7.7.-) (DNA primase 58 kDa subunit) (p58) [4932.FASTACCERV.txt] PRI2 SGDID:S0001528, Chr XI from 353138-354724, Verified ORF [yeastflysequest.fasta] Pri2p [Saccharomyces cerevisiae]gi\|486060\|emb\|CAA81880.1\| PRI2 [Saccharomyces cerevisiae]gi\|130899\|sp\|P20457\|PRI2_YEAST DNA primase large subunit (DNA primase 58 kDa subunit) (p58)gi\|101284\|pir\|\|A32497 DNA primase (EC 2.7.7.-) large chain - yeast (Sacc [nrdb_11152004_Chlamydomonas] PRI2 SGDID:S000001528, Chr XI from 353137-354723, Verified ORF, "Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] DNA primase large chain (P58) [Saccharomyces cerevisiae] [nr-271106-contam.fasta] DNA primase large subunit (DNA primase 58 kDa subunit) (DNA polymerase alpha:primase complex p58 subunit) (Pol alpha-primase complex p58 subunit) (DNA polymerase-primase complex p58 subunit) [nr-271106-contam.fasta] Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair; Pri2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] PR12 [nr-271106-contam.fasta] PRI2 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] DNA primase large subunit OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PRI2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] PRI2 SGDID:S000001528, Chr XI from 353493-355079, Genome Release 64-1-1, Verified ORF, "Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair" [yeast-030211-contam.fasta] PRI2 SGDID:S000001528, Chr XI from 353493-355079, Genome Release 64-2-1, Verified ORF, "Subunit of DNA primase; DNA primase is required for DNA synthesis and double-strand break repair" [20141208_orf_trans.fasta] TR5226\|c0_g1_i1\|g.3590 ORF TR5226\|c0_g1_i1\|g.3590 TR5226\|c0_g1_i1\|m.3590 type:complete len:529 (+) TR5226\|c0_g1_i1:148-1734(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YKL045W SGDID:S000001528, Chr XI from 353493-355079, Genome Release 64-1-1, Verified ORF, "Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] ID=asmbl_6728\|g.4058 ORF ID=asmbl_6728\|g.4058 ID=asmbl_6728\|m.4058 type:complete len:529 (+) ID=asmbl_6728:173-1759(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	528 amino acids
Gene Ontology:
Cellular Component:	nuclear envelope [IDA] nucleus [IDA] alpha DNA polymerase:primase complex [IDA]
Biological Process:	DNA replication initiation [IC] lagging strand elongation [IC] DNA replication [IMP] DNA synthesis involved in DNA repair [IMP] DNA replication, synthesis of RNA primer [IDA]
Molecular Function:	DNA primase activity [IDA ][IGI][IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MFRQSKRRIA SRKNFSSYDD IVKSELDVGN TNAANQIILS SSSSEEEKKL YARLYESKLS  60
   61 FYDLPPQGEI TLEQFEIWAI DRLKILLEIE SCLSRNKSIK EIETIIKPQF QKLLPFNTES 120
  121 LEDRKKDYYS HFILRLCFCR SKELREKFVR AETFLFKIRF NMLTSTDQTK FVQSLDLPLL 180
  181 QFISNEEKAE LSHQLYQTVS ASLQFQLNLN EEHQRKQYFQ QEKFIKLPFE NVIELVGNRL 240
  241 VFLKDGYAYL PQFQQLNLLS NEFASKLNQE LIKTYQYLPR LNEDDRLLPI LNHLSSGYTI 300
  301 ADFNQQKANQ FSENVDDEIN AQSVWSEEIS SNYPLCIKNL MEGLKKNHHL RYYGRQQLSL 360
  361 FLKGIGLSAD EALKFWSEAF TRNGNMTMEK FNKEYRYSFR HNYGLEGNRI NYKPWDCHTI 420
  421 LSKPRPGRGD YHGCPFRDWS HERLSAELRS MKLTQAQIIS VLDSCQKGEY TIACTKVFEM 480
  481 THNSASADLE IGEQTHIAHP NLYFERSRQL QKKQQKLEKE KLFNNGNH

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		POL1 POL12 PRI1 PRI2
View Details	(MIPS) Mewes HW, et al. (2004)		POL1 POL12 PRI1 PRI2
View Details	Gavin AC, et al. (2006)		POL1 POL12 PRI1 PRI2
View Details	Gavin AC, et al. (2002)		AHA1 ATP11 CAF130 CAF40 CCR4 CCT6 CDC36 CDC39 COP1 FAS2 GCN1 MIS1 MOT2 NOT3 NOT5 PDC1 POL1 POL12 POP2 PRI1 PRI2 RFC1 RFC3 RFC4 RFC5 RVB2 SAM1 SEC27 TFC7 TFP1 USO1 YRA1
View Details	Ho Y, et al. (2002)		ARO1 BCK1 CHS1 CMP2 DBP7 KOG1 KSP1 PRI2 PSY2 TPD3
View Details	Qiu et al. (2008)		DPB2 DPB3 DPB4 HCS1 MCM5 POL1 POL12 POL2 POL3 POL30 POL31 POL32 PRI1 PRI2 RFA1 RFA2 RFA3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	PRI2	Sundin BA, et al. (2004)
View Data	PRI2	Sundin BA, et al. (2004)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..139]	MSA	1.014976	View MSA. Confident ab initio structure predictions are available.
2	View Details	[140..220]	deduced	N/A	Confident ab initio structure predictions are available.
3	View Details	[221..468]	MSA	5.019968	View MSA. No confident structure predictions are available.
4	View Details	[469..528]	deduced	N/A	No confident structure predictions are available.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Protein Overview: PRI2

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: