Protein Overview: RFC2

General Information:
Name(s) found:	RFC2 / YJR068W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|841464\|gb\|AAC49061.1\| Rfc2p [NCBI NR] gi\|730502\|sp\|P40348.1\|RFC2_YEAST RecName: Full=Replication factor C subunit 2; Short=Replication factor C2; AltName: Full=Activator 1 41 kDa subunit [NCBI NR] gi\|6322528\|ref\|NP_012602.1\| Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and e [NCBI NR] pir\|\|S45531 replication factor C chain RFC2 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|498463\|dbj\|BAA05858.1\| Rfc2 protein [Saccharomyces cerevisiae] [NCBI NR] gi\|1019690\|gb\|AAB39294.1\| ORF YJR068w [NCBI NR] gi\|1015747\|emb\|CAA89596.1\| RFC2 [Saccharomyces cerevisiae] [NCBI NR] Replication factor C subunit 2 OS=Saccharomyces cerevisiae GN=RFC2 PE=1 SV=1 [Swiss-Prot] RFC2 DNA replication factor C, 41 KD subunit [mips] Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon [SGD] RFC2, Chr X from 567335-568396 [yeast_orfs2] Chr X from 567335-568396 [yeastorf3.fasta] Chr X from 567336-568397 [yeastorf4.fasta] Chr X from 567337-568398 [yeastorf5.fasta] (P40348) Activator 1 41 kDa subunit (Replication factor C subunit 2) (Replication factor C2) [4932.FASTACCERV.txt] RFC2 SGDID:S0003829, Chr X from 567557-568618, Verified ORF [yeastflysequest.fasta] Rfc2p [Saccharomyces cerevisiae]gi\|1015747\|emb\|CAA89596.1\| RFC2 [Saccharomyces cerevisiae]gi\|498463\|dbj\|BAA05858.1\| Rfc2 protein [Saccharomyces cerevisiae]gi\|841464\|gb\|AAC49061.1\| Rfc2pgi\|1019690\|gb\|AAB39294.1\| ORF YJR068wgi\|626873\|pir\|\|S45531 replic [nrdb_11152004_Chlamydomonas] RFC2 SGDID:S000003829, Chr X from 567557-568618, Verified ORF, "Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity fa [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] RFC2 SGDID:S000003829, Chr X from 567636-568697, Verified ORF, "Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity fa [yeast-contam.fasta] RFC2 SGDID:S000003829, Chr X from 567635-568696, Verified ORF, "Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity fa [yeast-200209-contam.fasta] Replication factor C subunit 2 (Replication factor C2) (Activator 1 41 kDa subunit) [nr-271106-contam.fasta] ORF YJR068w [nr-271106-contam.fasta] Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; Rfc2p [Saccharomyces [nr-271106-contam.fasta] Rfc2p [nr-271106-contam.fasta] RFC2 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Rfc2 protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] RFC2 SGDID:S000003829, Chr X from 567635-568696, Verified ORF, "Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon" [yeast-bovrabprots.fasta] RFC2 SGDID:S000003829, Chr X from 567636-568697, Verified ORF, "Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon" [yeast-contam_custom.fasta] [chemostatdb.fasta] Replication factor C subunit 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RFC2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] RFC2 SGDID:S000003829, Chr X from 567643-568704, Genome Release 64-1-1, Verified ORF, "Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon" [yeast-030211-contam.fasta] RFC2 SGDID:S000003829, Chr X from 567643-568704, Genome Release 64-2-1, Verified ORF, "Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon" [20141208_orf_trans.fasta] YJR068W SGDID:S000003829, Chr X from 567643-568704, Genome Release 64-1-1, Verified ORF, "Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	353 amino acids
Gene Ontology:
Cellular Component:	DNA replication factor C complex [IDA]
Biological Process:	sister chromatid cohesion [IPI] cell cycle checkpoint [IMP] leading strand elongation [IDA] mismatch repair [TAS]
Molecular Function:	DNA clamp loader activity [IDA ] purine nucleotide binding [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MFEGFGPNKK RKISKLAAEQ SLAQQPWVEK YRPKNLDEVT AQDHAVTVLK KTLKSANLPH  60
   61 MLFYGPPGTG KTSTILALTK ELYGPDLMKS RILELNASDE RGISIVREKV KNFARLTVSK 120
  121 PSKHDLENYP CPPYKIIILD EADSMTADAQ SALRRTMETY SGVTRFCLIC NYVTRIIDPL 180
  181 ASRCSKFRFK ALDASNAIDR LRFISEQENV KCDDGVLERI LDISAGDLRR GITLLQSASK 240
  241 GAQYLGDGKN ITSTQVEELA GVVPHDILIE IVEKVKSGDF DEIKKYVNTF MKSGWSAASV 300
  301 VNQLHEYYIT NDNFDTNFKN QISWLLFTTD SRLNNGTNEH IQLLNLLVKI SQL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ELG1 RFC1 RFC2 RFC3 RFC4 RFC5
View Details	(MIPS) Mewes HW, et al. (2004)		RFC1 RFC2 RFC3 RFC4 RFC5
View Details	Krogan NJ, et al. (2006)		ELG1 RFC1 RFC2 RFC3 RFC4 RFC5
View Details	Gavin AC, et al. (2006)		RFC1 RFC2
View Details	Gavin AC, et al. (2002)		CTF18 CTF8 DPB2 ELG1 OLA1 POL2 RFC1 RFC2 RFC3 RFC4 RFC5 TOP2 VPS13 YMR181C
View Details	Ho Y, et al. (2002)		ACH1 ACO1 ADE5,7 ADH2 AHA1 ARP2 ATP3 BRR2 CCT3 CLU1 CPA2 DUN1 ELG1 EXG1 FAA4 FET3 GUS1 HEF3 HSP104 IDH1 MDH2 NPA3 NTG1 PDI1 PGM2 PRB1 RAD24 RFC1 RFC2 RFC3 RFC4 RFC5 RNQ1 ROM2 RPC40 RPN5 RPT3 SAN1 SRP1 TCP1 TIF34 VAC8 YHR020W YIL108W YLR413W
View Details	Qiu et al. (2008)		CDC45 DPB11 DPB2 MCM6 POL31 RFC1 RFC2 RFC3 RFC4 RFC5

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	YMR057C	RFC2	2	Unpublished Fields Lab Data
View Screen	SPO21	RFC2	2	Unpublished Fields Lab Data
View Screen	MPC54	RFC2	2	Unpublished Fields Lab Data
View Screen	MPC54	RFC2	2	Unpublished Fields Lab Data
View Screen	ATG20	RFC2	2	Unpublished Fields Lab Data
View Screen	HBT1	RFC2	2	Unpublished Fields Lab Data
View Screen	SNX41	RFC2	2	Unpublished Fields Lab Data
View Screen	RGD2	RFC2	2	Unpublished Fields Lab Data
View Screen	TAM41	RFC2	2	Unpublished Fields Lab Data
View Screen	YJL147C	RFC2	2	Unpublished Fields Lab Data
View Screen	IML1	RFC2	2	Unpublished Fields Lab Data
View Screen	CAB3	RFC2	2	Hazbun TR, et al. (2003)
View Screen	CAB3	RFC2	2	Unpublished Fields Lab Data
View Screen	YKR078W	RFC2	2	Unpublished Fields Lab Data
View Screen	YOR289W	RFC2	1	Unpublished Fields Lab Data
View Screen	MPE1	RFC2	1	Unpublished Fields Lab Data
View Screen	HMG1	RFC2	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	RFC2	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..30] [193..262]	PSI-BLAST	679.315398	Replication factor C
2	View Details	[31..192] [263..353]	PSI-BLAST	679.315398	Replication factor C

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
DNA clamp loader activity	6.51495346697904	bayes_pls_golite062009
protein-DNA loading ATPase activity	6.40748863504898	bayes_pls_golite062009
DNA-dependent ATPase activity	2.72462789867829	bayes_pls_golite062009
binding	2.64537357529343	bayes_pls_golite062009
mismatched DNA binding	2.51625633651862	bayes_pls_golite062009
DNA helicase activity	2.2817164193758	bayes_pls_golite062009
nucleic acid binding	2.21184938313192	bayes_pls_golite062009
pyrophosphatase activity	2.13284212711072	bayes_pls_golite062009
DNA binding	1.94055378599526	bayes_pls_golite062009
ATP-dependent DNA helicase activity	1.88455339138556	bayes_pls_golite062009
	1.85506769032946	bayes_pls_golite062009
transcription regulator activity	1.83584389083042	bayes_pls_golite062009
helicase activity	1.70242731830224	bayes_pls_golite062009
guanine/thymine mispair binding	1.69026972999845	bayes_pls_golite062009
DNA insertion or deletion binding	1.68890613599528	bayes_pls_golite062009
RNA helicase activity	1.63960194106079	bayes_pls_golite062009
DNA replication origin binding	1.62491387774159	bayes_pls_golite062009
ATP-dependent RNA helicase activity	1.61735400124552	bayes_pls_golite062009
RNA-dependent ATPase activity	1.60404584546501	bayes_pls_golite062009
microtubule motor activity	1.35938517416356	bayes_pls_golite062009
structure-specific DNA binding	1.2539059700735	bayes_pls_golite062009
protein binding	1.22129154759482	bayes_pls_golite062009
purine ribonucleotide binding	1.21658213854831	bayes_pls_golite062009
ribonucleotide binding	1.21652144153182	bayes_pls_golite062009
purine nucleotide binding	1.21571071905795	bayes_pls_golite062009
nucleotide binding	1.20347573865566	bayes_pls_golite062009
purine NTP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
ATP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
motor activity	1.14667913749579	bayes_pls_golite062009
double-stranded DNA binding	1.117585097627	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
DNA-directed DNA polymerase activity	0.971476020912368	bayes_pls_golite062009
DNA polymerase activity	0.928091843881235	bayes_pls_golite062009
transcription factor activity	0.697256579145077	bayes_pls_golite062009
transcription repressor activity	0.677612020025357	bayes_pls_golite062009
guanyl nucleotide binding	0.656009904481476	bayes_pls_golite062009
guanyl ribonucleotide binding	0.64416740142962	bayes_pls_golite062009
GTP binding	0.643084645864898	bayes_pls_golite062009
single base insertion or deletion binding	0.629563765934841	bayes_pls_golite062009
single-stranded DNA binding	0.568457189190858	bayes_pls_golite062009
cytoskeletal protein binding	0.500462538667228	bayes_pls_golite062009
GTPase activity	0.406399103964327	bayes_pls_golite062009
DNA strand annealing activity	0.283577486331079	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.147579541298736	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.113504049290503	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.111293884322157	bayes_pls_golite062009
structural constituent of ribosome	0.089234257580272	bayes_pls_golite062009
3'-5' DNA helicase activity	0.0769112340058804	bayes_pls_golite062009
lysine N-acetyltransferase activity	0.0320943071400439	bayes_pls_golite062009
histone acetyltransferase activity	0.0320943071400439	bayes_pls_golite062009
double-strand/single-strand DNA junction binding	0.0136229525922795	bayes_pls_golite062009
actin binding	0.00482938540552702	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2