Protein Overview: MYO2

General Information:
Name(s) found:	MYO2 / YOR326W [SGD]
Description(s) found: Found 26 descriptions. SHOW ALL	One of two type V myosin motors (along with MYO4) involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle [SGD]
Organism:	Saccharomyces cerevisiae
Length:	1574 amino acids
Gene Ontology:
Cellular Component:	incipient cellular bud site [IDA] cellular bud tip [IDA] cellular bud neck [IDA] fungal-type vacuole membrane [IDA] myosin V complex [ISS] mating projection tip [IDA] vesicle [IDA] filamentous actin [IDA]
Biological Process:	mitochondrion inheritance [IGI ][IMP][IPI] vacuole inheritance [IGI ][IMP][IPI] establishment of mitotic spindle orientation [IMP][IPI] unidimensional cell growth [IMP] vesicle-mediated transport [IGI][IMP] Golgi inheritance [IMP] vesicle transport along actin filament [IEP ][IMP ] peroxisome inheritance [IMP][IPI] membrane addition at site of cytokinesis [IEP][IGI][IMP] budding cell apical bud growth [IMP]
Molecular Function:	actin filament binding [IDA] calmodulin binding [IDA] microfilament motor activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSFEVGTRCW YPHKELGWIG AEVIKNEFND GKYHLELQLE DDEIVSVDTK DLNNDKDQSL  60
   61 PLLRNPPILE ATEDLTSLSY LNEPAVLHAI KQRYSQLNIY TYSGIVLIAT NPFDRVDQLY 120
  121 TQDMIQAYAG KRRGELEPHL FAIAEEAYRL MKNDKQNQTI VVSGESGAGK TVSAKYIMRY 180
  181 FASVEEENSA TVQHQVEMSE TEQKILATNP IMEAFGNAKT TRNDNSSRFG KYLEILFDKD 240
  241 TSIIGARIRT YLLERSRLVY QPPIERNYHI FYQLMAGLPA QTKEELHLTD ASDYFYMNQG 300
  301 GDTKINGIDD AKEYKITVDA LTLVGITKET QHQIFKILAA LLHIGNIEIK KTRNDASLSA 360
  361 DEPNLKLACE LLGIDAYNFA KWVTKKQIIT RSEKIVSNLN YSQALVAKDS VAKFIYSALF 420
  421 DWLVENINTV LCNPAVNDQI SSFIGVLDIY GFEHFEKNSF EQFCINYANE KLQQEFNQHV 480
  481 FKLEQEEYVK EEIEWSFIEF NDNQPCIDLI ENKLGILSLL DEESRLPAGS DESWTQKLYQ 540
  541 TLDKSPTNKV FSKPRFGQTK FIVSHYALDV AYDVEGFIEK NRDTVSDGHL EVLKASTNET 600
  601 LINILEGLEK AAKKLEEAKK LELEQAGSKK PGPIRTVNRK PTLGSMFKQS LIELMNTINS 660
  661 TNVHYIRCIK PNADKEAWQF DNLMVLSQLR ACGVLETIRI SCAGFPSRWT FEEFVLRYYI 720
  721 LIPHEQWDLI FKKKETTEED IISVVKMILD ATVKDKSKYQ IGNTKIFFKA GMLAYLEKLR 780
  781 SNKMHNSIVM IQKKIRAKYY RKQYLQISQA IKYLQNNIKG FIIRQRVNDE MKVNCATLLQ 840
  841 AAYRGHSIRA NVFSVLRTIT NLQKKIRKEL KQRQLKQEHE YNAAVTIQSK VRTFEPRSRF 900
  901 LRTKKDTVVV QSLIRRRAAQ RKLKQLKADA KSVNHLKEVS YKLENKVIEL TQNLASKVKE 960
  961 NKEMTERIKE LQVQVEESAK LQETLENMKK EHLIDIDNQK SKDMELQKTI ENNLQSTEQT1020
 1021 LKDAQLELED MVKQHDELKE ESKKQLEELE QTKKTLVEYQ TLNGDLQNEV KSLKEEIARL1080
 1081 QTAMSLGTVT TSVLPQTPLK DVMGGGASNF NNMMLENSDL SPNDLNLKSR STPSSGNNHI1140
 1141 DSLSVDRENG VNATQINEEL YRLLEDTEIL NQEITEGLLK GFEVPDAGVA IQLSKRDVVY1200
 1201 PARILIIVLS EMWRFGLTKQ SESFLAQVLT TIQKVVTQLK GNDLIPSGVF WLANVRELYS1260
 1261 FVVFALNSIL TEETFKNGMT DEEYKEYVSL VTELKDDFEA LSYNIYNIWL KKLQKQLQKK1320
 1321 AINAVVISES LPGFSAGETS GFLNKIFANT EEYTMDDILT FFNSIYWCMK SFHIENEVFH1380
 1381 AVVTTLLNYV DAICFNELIM KRNFLSWKRG LQLNYNVTRL EEWCKTHGLT DGTECLQHLI1440
 1441 QTAKLLQVRK YTIEDIDILR GICYSLTPAQ LQKLISQYQV ADYESPIPQE ILRYVADIVK1500
 1501 KEAALSSSGN DSKGHEHSSS IFITPETGPF TDPFSLIKTR KFDQVEAYIP AWLSLPSTKR1560
 1561 IVDLVAQQVV QDGH

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CMD1 CRN1 MLC1 MYO2 MYO4 SHE4
View Details	(MIPS) Mewes HW, et al. (2004)		MYO1 MYO2 MYO3 MYO4 MYO5 SMY1 SMY2
View Details	Krogan NJ, et al. (2006)		ATG33 BUB1 CUL3 GUT1 ISM1 MEC3 MLC1 MYO2 MYO4 RGA1 RSB1 SAT4 SHE2 SHE3 SHE4 SRC1 SSH4 STT4 TOF2 TVP18 YDR186C YOL098C
View Details	Gavin AC, et al. (2006)		MYO2 SHE4
View Details	Gavin AC, et al. (2002)		ACE2 ACT1 ADH1 ALT2 ARX1 BCK1 CDC11 CMD1 CSL4 CTR9 DIS3 DRE2 ECM16 ECM30 ENP2 GCN1 HSC82 IML1 KAP123 MAM33 MLC1 MLC2 MNL1 MTR3 MTR4 MYO1 MYO2 MYO4 NIP1 NMD5 RGC1 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 SAM1 SEC3 SHE2 SHE3 SHE4 SKI6 SKI7 SRP1 UBP15 UTP10 YBL044W YEF3 YJR098C YNL313C
View Details	Ho Y, et al. (2002)		ADR1 BBC1 CCT2 CCT3 CCT5 CCT6 CDC25 CDC39 CDC53 CKI1 CMD1 CMK2 CMP2 COF1 CPR1 CRM1 DUR1,2 ECM29 ECM33 EDE1 FAA4 FZO1 GAL3 GCN1 GCR2 GIN4 HCH1 HOM3 HRT1 HUL5 HYP2 ILS1 IPP1 IRA1 IRA2 MDN1 MKT1 MLC1 MMS1 MYO2 MYO3 MYO4 MYO5 NAP1 NEW1 PEX7 PFK1 PGM2 PMA2 PMD1 PRS2 PRS3 PRS5 PST2 RIM11 RPA190 RPN1 RPN6 SHE3 SHE4 SOD1 SPC110 TPS1 TSL1 UBA1 UBI4 UBR1 VAS1 VPS13 YAR009C YDR170W-A YER138C YER160C YGP1 YJR027W YJR028W YLR035C-A YNK1
View Details	Qiu et al. (2008)		ABP140 ACT1 BEM1 BNI1 BNI4 BNR1 BUD6 CAP1 CAP2 CIK1 DYN1 DYN3 HOF1 HSK3 KAR3 KIP3 MLC1 MLC2 MYO1 MYO2 MYO3 MYO4 MYO5 PAC11 PEA2 PXL1 SAC6 SCP1 SMY1 SMY2 SPA2 SPH1 TPM1 TPM2

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	TTI2	No Comments	Hazbun TR, et al. (2003)
View Run	CAB3	No Comments	Hazbun TR, et al. (2003)
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	NUP1	MYO2	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	MYO2	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..63]	PSI-BLAST	2630.0	Myosin S1 fragment, N-terminal domain; Myosin S1, motor domain
2	View Details	[64..342] [417..452] [653..697]	PSI-BLAST	2630.0	Myosin S1 fragment, N-terminal domain; Myosin S1, motor domain
3	View Details	[343..416] [453..483] [498..652] [698..701]	PSI-BLAST	2630.0	Myosin S1 fragment, N-terminal domain; Myosin S1, motor domain
4	View Details	[484..497] [702..783]	PSI-BLAST	2630.0	Myosin S1 fragment, N-terminal domain; Myosin S1, motor domain
5	View Details	[784..844]	PSI-BLAST	12.522879	SCALLOP MYOSIN REGULATORY DOMAIN
6	View Details	[845..1009]	PSI-BLAST	4.0	Colicin Ia; Colicin Ia, N-terminal domain
7	View Details	[1010..1327]	PSI-BLAST	4.0	Colicin Ia; Colicin Ia, N-terminal domain
8	View Details	[1328..1380]	deduced	N/A	No confident structure predictions are available.
9	View Details	[1381..1509]	Pfam	46.657577	DIL domain No confident structure predictions are available.
10	View Details	[1510..1574]	deduced	N/A	Confident ab initio structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2