Protein Overview: KAR3

General Information:
Name(s) found:	KAR3 / YPR141C [SGD]
Description(s) found: Found 29 descriptions. SHOW ALL	Minus-end-directed microtubule motor that functions in mitosis and meiosis, localizes to the spindle pole body and localization is dependent on functional Cik1p, required for nuclear fusion during mating; potential Cdc28p substrate [SGD]
Organism:	Saccharomyces cerevisiae
Length:	729 amino acids
Gene Ontology:
Cellular Component:	spindle pole body [IDA ] microtubule [TAS]
Biological Process:	mitotic sister chromatid cohesion [IGI][IMP] mitosis [IGI] karyogamy involved in conjugation with cellular fusion [IMP] meiosis [IDA][IMP]
Molecular Function:	microtubule motor activity [TAS] motor activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MESLPRTPTK GRSTQHLSTP SPKNDILAMN GHKRRNTTTP PPKHTLLKPQ RTDIHRHSLA  60
   61 SQSRISMSPN RELLKNYKGT ANLIYGNQKS NSGVTSFYKE NVNELNRTQA ILFEKKATLD 120
  121 LLKDELTETK EKINAVNLKF ETLREEKIKI EQQLNLKNNE LISIKEEFLS KKQFMNEGHE 180
  181 IHLKQLAASN KKELKQMENE YKTKIEKLKF MKIKQFENER ASLLDKIEEV RNKITMNPST 240
  241 LQEMLNDVEQ KHMLEKEEWL TEYQSQWKKD IELNNKHMQE IESIKKEIEN TLKPELAEKK 300
  301 KLLTEKRNAY EAIKVKVKEK EEETTRLRDE VALKQKTNLE TLEKIKELEE YIKDTELGMK 360
  361 ELNEILIKEE TVRRTLHNEL QELRGNIRVY CRIRPALKNL ENSDTSLINV NEFDDNSGVQ 420
  421 SMEVTKIQNT AQVHEFKFDK IFDQQDTNVD VFKEVGQLVQ SSLDGYNVCI FAYGQTGSGK 480
  481 TFTMLNPGDG IIPSTISHIF NWINKLKTKG WDYKVNCEFI EIYNENIVDL LRSDNNNKED 540
  541 TSIGLKHEIR HDQETKTTTI TNVTSCKLES EEMVEIILKK ANKLRSTAST ASNEHSSRSH 600
  601 SIFIIHLSGS NAKTGAHSYG TLNLVDLAGS ERINVSQVVG DRLRETQNIN KSLSCLGDVI 660
  661 HALGQPDSTK RHIPFRNSKL TYLLQYSLTG DSKTLMFVNI SPSSSHINET LNSLRFASKV 720
  721 NSTRLVSRK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		AME1 ARP1 BIK1 CIK1 CIN8 COG7 DYN1 JNM1 KAR3 KAR5 KIP1 KIP2 KIP3 LIN1 NUF2 SPC19 SPC34 STU1 STU2 TID3
View Details	Qiu et al. (2008)		AME1 BBP1 BIK1 BIM1 CIK1 CIN8 CNM67 COG7 DYN1 DYN3 HSK3 KAR3 KAR9 KIP1 KIP2 KIP3 LIN1 MYO2 NUF2 PAC1 PAC11 SGO1 SLK19 SPC19 SPC24 SPC34 STU1 STU2 TID3 VIK1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	FUN19	KAR3	2	Unpublished Fields Lab Data
View Screen	RBG1	KAR3	2	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	KAR3	Huh WK, et al. (2003)
View Data	KAR3 and SPC42	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..371]	PSI-BLAST	85.980454	Heavy meromyosin subfragment
2	View Details	[372..729]	PSI-BLAST	10120.0	Kinesin motor Ncd (non-claret disjunctional)

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
microtubule motor activity	7.68336791348661	bayes_pls_golite062009
plus-end-directed microtubule motor activity	4.97507344344589	bayes_pls_golite062009
microfilament motor activity	4.176620576177	bayes_pls_golite062009
motor activity	4.04927796049806	bayes_pls_golite062009
nucleoside-triphosphatase activity	2.95079405147356	bayes_pls_golite062009
pyrophosphatase activity	2.87427509025394	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	2.85499564954033	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	2.85178667290149	bayes_pls_golite062009
actin-dependent ATPase activity	2.77140616759165	bayes_pls_golite062009
tubulin binding	2.67743463093593	bayes_pls_golite062009
binding	2.64537357529343	bayes_pls_golite062009
microtubule binding	2.59155206524122	bayes_pls_golite062009
transporter activity	2.58140740499725	bayes_pls_golite062009
cytoskeletal protein binding	2.4101693596493	bayes_pls_golite062009
transmembrane transporter activity	2.39695871109024	bayes_pls_golite062009
ATPase activity	2.39535714892224	bayes_pls_golite062009
ATPase activity, coupled	2.18396165590908	bayes_pls_golite062009
ATP-dependent DNA helicase activity	2.07967285635739	bayes_pls_golite062009
DNA clamp loader activity	1.984885722076	bayes_pls_golite062009
actin binding	1.75397031486306	bayes_pls_golite062009
hydrolase activity	1.57549510726226	bayes_pls_golite062009
helicase activity	1.33212695797068	bayes_pls_golite062009
substrate-specific transporter activity	1.29730375126024	bayes_pls_golite062009
protein binding	1.22129154759482	bayes_pls_golite062009
DNA-dependent ATPase activity	1.07479235523606	bayes_pls_golite062009
purine nucleotide binding	0.929901285538132	bayes_pls_golite062009
nucleotide binding	0.922442819194247	bayes_pls_golite062009
purine ribonucleotide binding	0.920987930180051	bayes_pls_golite062009
ribonucleotide binding	0.920926158369519	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	0.906554174337376	bayes_pls_golite062009
purine NTP-dependent helicase activity	0.89390151223569	bayes_pls_golite062009
ATP-dependent helicase activity	0.89390151223569	bayes_pls_golite062009
nucleic acid binding	0.777787753097919	bayes_pls_golite062009
actin filament binding	0.728264616959762	bayes_pls_golite062009
microtubule plus-end binding	0.673980113049624	bayes_pls_golite062009
structure-specific DNA binding	0.579644950922817	bayes_pls_golite062009
tubulin-dependent ATPase activity	0.553754516119488	bayes_pls_golite062009
kinetochore binding	0.505370954958131	bayes_pls_golite062009
DNA binding	0.479412476059825	bayes_pls_golite062009
GTPase activity	0.437665353870877	bayes_pls_golite062009
active transmembrane transporter activity	0.434775387083003	bayes_pls_golite062009
ATP binding	0.41092997612902	bayes_pls_golite062009
adenyl ribonucleotide binding	0.385813421701492	bayes_pls_golite062009
adenyl nucleotide binding	0.377870145186619	bayes_pls_golite062009
ion transmembrane transporter activity	0.355466224802969	bayes_pls_golite062009
histone acetyltransferase activity	0.328186246219424	bayes_pls_golite062009
lysine N-acetyltransferase activity	0.328186246219424	bayes_pls_golite062009
transcription regulator activity	0.312171320541728	bayes_pls_golite062009
primary active transmembrane transporter activity	0.294474570607914	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	0.282352478532746	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	0.220608508617331	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	0.219533217661421	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	0.219157471322311	bayes_pls_golite062009
DNA helicase activity	0.17029946873911	bayes_pls_golite062009
mismatched DNA binding	0.13866566261611	bayes_pls_golite062009
minus-end-directed microtubule motor activity	0.068779631341469	bayes_pls_golite062009
cation transmembrane transporter activity	0.0254369227200564	bayes_pls_golite062009
structural constituent of cytoskeleton	0.0111338193708552	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2