KAR3-encoded kinesin is a minus-end-directed motor that functions with centromere binding proteins (CBF3) on an in vitro yeast kinetochore

Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7212-6. doi: 10.1073/pnas.91.15.7212.

Abstract

We have used in vitro motility assays to investigate the mechanism of kinetochore function in the budding yeast Saccharomyces cerevisiae. Functional centromeric DNA plus a tripartite centromere binding protein complex, CBF3, was found to be necessary but not sufficient for in vitro kinetochore activity. A fourth required component was identified as the motor protein Kar3p, a previously reported yeast kinesin known to be involved in karyogamy and mitosis. Our data support genetic evidence suggesting that Kar3p is a kinetochore-associated motor and imply that CBF3 plays a regulatory role in kinetochore function.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Centromere / physiology*
  • Chromosomes, Fungal
  • DNA-Binding Proteins / physiology*
  • Fungal Proteins / physiology*
  • Kinesins / physiology*
  • Kinetochores
  • Microtubule-Associated Proteins*
  • Nuclear Proteins*
  • Saccharomyces cerevisiae / physiology*
  • Saccharomyces cerevisiae Proteins*

Substances

  • CEP3 protein, S cerevisiae
  • DNA-Binding Proteins
  • Fungal Proteins
  • KAR3 protein, S cerevisiae
  • Microtubule-Associated Proteins
  • Nuclear Proteins
  • Saccharomyces cerevisiae Proteins
  • Kinesins