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View Structure Prediction Details

Protein: KAR3
Organism: Saccharomyces cerevisiae
Length: 729 amino acids
Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076



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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for KAR3.

Description E-value Query
Range
Subject
Range
KAR3 - Minus-end-directed microtubule motor that functions in mitosis and meiosis, localizes to the spindle...
gi|190408065 - gi|190408065|gb|EDV11330.1| hypothetical protein SCRG_02616 [Saccharomyces cerevisiae RM11-1a]
KAR3_YEAST - Kinesin-like protein KAR3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=KAR3 PE=1 SV=1
gi|151942914 - gi|151942914|gb|EDN61260.1| kinesin-like nuclear fusion protein [Saccharomyces cerevisiae YJM789]
0.0 [1..729] [1..729]
gi|7507776 - gi|7507776|pir||T16867 probable cytochrome P450 T13C5.1 [similarity] - Caenorhabditis elegans
0.0 [59..729] [579..1216]
ATK2_ARATH - Kinesin-2 OS=Arabidopsis thaliana GN=ATK2 PE=2 SV=1
0.0 [25..728] [38..729]

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Predicted Domain #1
Region A:
Residues: [1-371]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MESLPRTPTK GRSTQHLSTP SPKNDILAMN GHKRRNTTTP PPKHTLLKPQ RTDIHRHSLA  60
   61 SQSRISMSPN RELLKNYKGT ANLIYGNQKS NSGVTSFYKE NVNELNRTQA ILFEKKATLD 120
  121 LLKDELTETK EKINAVNLKF ETLREEKIKI EQQLNLKNNE LISIKEEFLS KKQFMNEGHE 180
  181 IHLKQLAASN KKELKQMENE YKTKIEKLKF MKIKQFENER ASLLDKIEEV RNKITMNPST 240
  241 LQEMLNDVEQ KHMLEKEEWL TEYQSQWKKD IELNNKHMQE IESIKKEIEN TLKPELAEKK 300
  301 KLLTEKRNAY EAIKVKVKEK EEETTRLRDE VALKQKTNLE TLEKIKELEE YIKDTELGMK 360
  361 ELNEILIKEE T

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 85.980454
Match: 1i84S_
Description: Heavy meromyosin subfragment
Matching Structure (courtesy of the PDB):

Predicted Domain #2
Region A:
Residues: [372-729]
      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 VRRTLHNELQ ELRGNIRVYC RIRPALKNLE NSDTSLINVN EFDDNSGVQS MEVTKIQNTA  60
   61 QVHEFKFDKI FDQQDTNVDV FKEVGQLVQS SLDGYNVCIF AYGQTGSGKT FTMLNPGDGI 120
  121 IPSTISHIFN WINKLKTKGW DYKVNCEFIE IYNENIVDLL RSDNNNKEDT SIGLKHEIRH 180
  181 DQETKTTTIT NVTSCKLESE EMVEIILKKA NKLRSTASTA SNEHSSRSHS IFIIHLSGSN 240
  241 AKTGAHSYGT LNLVDLAGSE RINVSQVVGD RLRETQNINK SLSCLGDVIH ALGQPDSTKR 300
  301 HIPFRNSKLT YLLQYSLTGD SKTLMFVNIS PSSSHINETL NSLRFASKVN STRLVSRK

[Run NCBI BLAST on this sequence.]

Detection Method: PSI-BLAST
Confidence: 10120.0
Match: 1f9tA_
Description: Kinesin motor Ncd (non-claret disjunctional)
Matching Structure (courtesy of the PDB):

Predicted functions:

Term Confidence Notes
microtubule motor activity 7.68336791348661 bayes_pls_golite062009
plus-end-directed microtubule motor activity 4.97507344344589 bayes_pls_golite062009
microfilament motor activity 4.176620576177 bayes_pls_golite062009
motor activity 4.04927796049806 bayes_pls_golite062009
nucleoside-triphosphatase activity 2.95079405147356 bayes_pls_golite062009
pyrophosphatase activity 2.87427509025394 bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides 2.85499564954033 bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides 2.85178667290149 bayes_pls_golite062009
actin-dependent ATPase activity 2.77140616759165 bayes_pls_golite062009
tubulin binding 2.67743463093593 bayes_pls_golite062009
binding 2.64537357529343 bayes_pls_golite062009
microtubule binding 2.59155206524122 bayes_pls_golite062009
transporter activity 2.58140740499725 bayes_pls_golite062009
cytoskeletal protein binding 2.4101693596493 bayes_pls_golite062009
transmembrane transporter activity 2.39695871109024 bayes_pls_golite062009
ATPase activity 2.39535714892224 bayes_pls_golite062009
ATPase activity, coupled 2.18396165590908 bayes_pls_golite062009
ATP-dependent DNA helicase activity 2.07967285635739 bayes_pls_golite062009
DNA clamp loader activity 1.984885722076 bayes_pls_golite062009
actin binding 1.75397031486306 bayes_pls_golite062009
hydrolase activity 1.57549510726226 bayes_pls_golite062009
helicase activity 1.33212695797068 bayes_pls_golite062009
substrate-specific transporter activity 1.29730375126024 bayes_pls_golite062009
protein binding 1.22129154759482 bayes_pls_golite062009
DNA-dependent ATPase activity 1.07479235523606 bayes_pls_golite062009
purine nucleotide binding 0.929901285538132 bayes_pls_golite062009
nucleotide binding 0.922442819194247 bayes_pls_golite062009
purine ribonucleotide binding 0.920987930180051 bayes_pls_golite062009
ribonucleotide binding 0.920926158369519 bayes_pls_golite062009
substrate-specific transmembrane transporter activity 0.906554174337376 bayes_pls_golite062009
purine NTP-dependent helicase activity 0.89390151223569 bayes_pls_golite062009
ATP-dependent helicase activity 0.89390151223569 bayes_pls_golite062009
nucleic acid binding 0.777787753097919 bayes_pls_golite062009
actin filament binding 0.728264616959762 bayes_pls_golite062009
microtubule plus-end binding 0.673980113049624 bayes_pls_golite062009
structure-specific DNA binding 0.579644950922817 bayes_pls_golite062009
tubulin-dependent ATPase activity 0.553754516119488 bayes_pls_golite062009
kinetochore binding 0.505370954958131 bayes_pls_golite062009
DNA binding 0.479412476059825 bayes_pls_golite062009
GTPase activity 0.437665353870877 bayes_pls_golite062009
active transmembrane transporter activity 0.434775387083003 bayes_pls_golite062009
ATP binding 0.41092997612902 bayes_pls_golite062009
adenyl ribonucleotide binding 0.385813421701492 bayes_pls_golite062009
adenyl nucleotide binding 0.377870145186619 bayes_pls_golite062009
ion transmembrane transporter activity 0.355466224802969 bayes_pls_golite062009
histone acetyltransferase activity 0.328186246219424 bayes_pls_golite062009
lysine N-acetyltransferase activity 0.328186246219424 bayes_pls_golite062009
transcription regulator activity 0.312171320541728 bayes_pls_golite062009
primary active transmembrane transporter activity 0.294474570607914 bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity 0.282352478532746 bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances 0.220608508617331 bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances 0.219533217661421 bayes_pls_golite062009
ATPase activity, coupled to movement of substances 0.219157471322311 bayes_pls_golite062009
DNA helicase activity 0.17029946873911 bayes_pls_golite062009
mismatched DNA binding 0.13866566261611 bayes_pls_golite062009
minus-end-directed microtubule motor activity 0.068779631341469 bayes_pls_golite062009
cation transmembrane transporter activity 0.0254369227200564 bayes_pls_golite062009
structural constituent of cytoskeleton 0.0111338193708552 bayes_pls_golite062009

YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle