Protein Overview: PAC1

General Information:
Name(s) found:	PAC1 / YOR269W [SGD]
Description(s) found: Found 28 descriptions. SHOW ALL	Protein involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; synthetic lethal with bni1; homolog of human LIS1 [SGD]
Organism:	Saccharomyces cerevisiae
Length:	494 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA] cytoplasmic microtubule [IDA]
Biological Process:	nuclear migration along microtubule [IMP]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTNWQQQLPL TDTQKNELDK SVLRYLNWNY KQTVRHEHAQ DYESVRHAIV TLSGFLLQES  60
   61 VDRQEFISNN DTSNESMVDI DELLLPKKWN SIVRLQKKII ELEQNTETLV SQIKDLNTQV 120
  121 SELAQFKPTT SNGTSAHNVL KWIPRNLPSC LINVESSVTS VKLHPNLPIV FVATDHGKLY 180
  181 AFDLFNYTIP LASLQSHTKA ITSMDVLFTN YTNSSKKNYL VIVTASKDLQ IHVFKWVSEE 240
  241 CKFQQIRSLL GHEHIVSAVK IWQKNNDVHI ASCSRDQTVK IWDFHNGWSL KTFQPHSQWV 300
  301 RSIDVLGDYI ISGSHDTTLR LTHWPSGNGL SVGTGHEFPI EKVKFIHFIE DSPEIRFRTP 360
  361 STDRYKNWGM QYCVSASRDR TIKIWEIPLP TLMAHRAPIP NPTDSNFRCV LTLKGHLSWV 420
  421 RDISIRGQYL FSCADDKSVR CWDLNTGQCL HVWEKLHTGF VNCLDLDVDF DSNVTPRQMM 480
  481 VTGGLDCKSN VFMR

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		ARC18 ARC19 ARC35 ARC40 ARP2 ARP3 PAC1 TAP42
View Details	Qiu et al. (2008)		BIM1 CIK1 DYN1 DYN3 KAR3 KIP2 PAC1

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CLN3	PAC1	2	Unpublished Fields Lab Data
View Screen	NSE5	PAC1	2	Hazbun TR, et al. (2003)
View Screen	PAC1	SEC15	2	Unpublished Fields Lab Data
View Screen	PAC1	CPR6	2	Unpublished Fields Lab Data
View Screen	PAC1	HSC82	2	Unpublished Fields Lab Data
View Screen	PAC1	STI1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	PAC1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..153]	PSI-BLAST	24.522879	Groucho/tle1, C-terminal domain
2	View Details	[154..494]	PSI-BLAST	127.39794	Tup1, C-terminal domain

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
dynactin binding	7.11157019623125	bayes_pls_golite062009
dynein heavy chain binding	6.59987903164261	bayes_pls_golite062009
histone methyltransferase activity (H3-K4 specific)	4.71992275913384	bayes_pls_golite062009
actin binding	3.86142918698875	bayes_pls_golite062009
cytoskeletal protein binding	3.1056443224755	bayes_pls_golite062009
tubulin binding	2.76945430061825	bayes_pls_golite062009
microtubule binding	2.72684103222924	bayes_pls_golite062009
dynein binding	2.54821997913979	bayes_pls_golite062009
binding	2.42570478946173	bayes_pls_golite062009
snoRNA binding	2.20077763915561	bayes_pls_golite062009
S-adenosylmethionine-dependent methyltransferase activity	2.18628561734369	bayes_pls_golite062009
actin filament binding	2.0735069176589	bayes_pls_golite062009
histone methyltransferase activity	1.85632145559025	bayes_pls_golite062009
transporter activity	1.65168247201205	bayes_pls_golite062009
nucleic acid binding	1.40004045504145	bayes_pls_golite062009
platelet-activating factor acetyltransferase activity	1.36229564654648	bayes_pls_golite062009
protein methyltransferase activity	1.35170368882651	bayes_pls_golite062009
transcription regulator activity	1.25687092973217	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	1.22384651674093	bayes_pls_golite062009
protein binding	1.21879977456466	bayes_pls_golite062009
histone-lysine N-methyltransferase activity	1.15313682561559	bayes_pls_golite062009
lysine N-methyltransferase activity	1.15313682561559	bayes_pls_golite062009
protein-lysine N-methyltransferase activity	1.15313682561559	bayes_pls_golite062009
microtubule plus-end binding	1.01166033978052	bayes_pls_golite062009
myosin binding	0.989691178611801	bayes_pls_golite062009
protein transporter activity	0.964340340346761	bayes_pls_golite062009
	0.88172763425403	bayes_pls_golite062009
myosin heavy chain kinase activity	0.848140419140051	bayes_pls_golite062009
DNA binding	0.711274653406079	bayes_pls_golite062009
dynein intermediate chain binding	0.707581908546932	bayes_pls_golite062009
histone binding	0.685319917508843	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	0.426429095658617	bayes_pls_golite062009
kinase activity	0.387572734234845	bayes_pls_golite062009
molecular transducer activity	0.367729389382533	bayes_pls_golite062009
signal transducer activity	0.367729389382533	bayes_pls_golite062009
structural molecule activity	0.254012051644049	bayes_pls_golite062009
transcription factor activity	0.243992994917491	bayes_pls_golite062009
protein kinase activity	0.229242350006544	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.226335610368636	bayes_pls_golite062009
ligase activity	0.20682813699621	bayes_pls_golite062009
enzyme regulator activity	0.161639372576406	bayes_pls_golite062009
chromatin binding	0.145491963066342	bayes_pls_golite062009
substrate-specific transporter activity	0.121183207943714	bayes_pls_golite062009
myosin II binding	0.12060669039328	bayes_pls_golite062009
structural constituent of cytoskeleton	0.118975415284669	bayes_pls_golite062009
RNA binding	0.0914092269020899	bayes_pls_golite062009
transcription repressor activity	0.084112683169759	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.00585398682921312	bayes_pls_golite062009
N-methyltransferase activity	0.00335766408304172	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2