Protein Overview: HRP1

General Information:
Name(s) found:	HRP1 / YOL123W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324449\|ref\|NP_014518.1\| Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal s [NCBI NR] gi\|2500574\|sp\|Q99383.1\|HRP1_YEAST RecName: Full=Nuclear polyadenylated RNA-binding protein 4; AltName: Full=Cleavage factor IB; Short=CFIB [NCBI NR] pir\|\|S66820 heterogeneous nuclear ribonucleoprotein HRP1 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1657691\|gb\|AAB18142.1\| Hrp1p [Saccharomyces cerevisiae] [NCBI NR] gi\|1550721\|emb\|CAA64546.1\| RNA binding protein [Saccharomyces cerevisiae] [NCBI NR] gi\|1420003\|emb\|CAA99142.1\| HRP1 [Saccharomyces cerevisiae] [NCBI NR] gi\|1016290\|gb\|AAA79097.1\| nuclear polyadenylated RNA-binding protein [NCBI NR] (Q99383) Nuclear polyadenylated RNA-binding protein 4 [Swiss-Prot] Nuclear polyadenylated RNA-binding protein 4 OS=Saccharomyces cerevisiae GN=HRP1 PE=1 SV=1 [Swiss-Prot] HRP1 CF Ib (RNA3` Cleavage factor Ib) [mips] Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences [SGD] (Q99383) Nuclear polyadenylated RNA-binding protein NAB4 [4932.FASTACCERV.txt] Chr XV from 87843-89447 [hmr_yeast5_DM3-1_pombe.fasta] HRP1, Chr XV from 87843-89447 [yeast_orfs2] HRP1 SGDID:S0005483, Chr XV from 87843-89447, Verified ORF [yeastflysequest.fasta] Hrp1p [Saccharomyces cerevisiae]gi\|1550721\|emb\|CAA64546.1\| RNA binding protein [Saccharomyces cerevisiae]gi\|1420003\|emb\|CAA99142.1\| HRP1 [Saccharomyces cerevisiae]gi\|2131274\|pir\|\|S66820 heterogeneous nuclear ribonucleoprotein HRP1 - yeast (Saccharomyc [nrdb_11152004_Chlamydomonas] HRP1 SGDID:S000005483, Chr XV from 87843-89447, Verified ORF, "Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family mem [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] HRP1 SGDID:S000005483, Chr XV from 87844-89448, Verified ORF, "Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family mem [yeast-contam.fasta] nuclear polyadenylated RNA-binding protein [nr-271106-contam.fasta] RNA binding protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Nuclear polyadenylated RNA-binding protein 4 (Cleavage factor IB) (CFIB) [nr-271106-contam.fasta] Hrp1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences; Hrp1p [Saccharomyc [nr-271106-contam.fasta] HRP1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] HRP1 SGDID:S000005483, Chr XV from 87844-89448, Verified ORF, "Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Nuclear polyadenylated RNA-binding protein 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HRP1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] HRP1 SGDID:S000005483, Chr XV from 87844-89448, Genome Release 64-1-1, Verified ORF, "Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences" [yeast-030211-contam.fasta] HRP1 SGDID:S000005483, Chr XV from 87844-89448, Genome Release 64-2-1, Verified ORF, "Subunit of cleavage factor I; cleavage factor I is a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences; required for genome stability" [20141208_orf_trans.fasta] TR1669\|c0_g1_i1\|g.1198 ORF TR1669\|c0_g1_i1\|g.1198 TR1669\|c0_g1_i1\|m.1198 type:complete len:535 (+) TR1669\|c0_g1_i1:388-1992(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR4839\|c0_g1_i1\|g.3210 ORF TR4839\|c0_g1_i1\|g.3210 TR4839\|c0_g1_i1\|m.3210 type:complete len:535 (+) TR4839\|c0_g1_i1:398-2002(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YOL123W SGDID:S000005483, Chr XV from 87844-89448, Genome Release 64-1-1, Verified ORF, "Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_2111\|g.2065 ORF asmbl_2111\|g.2065 asmbl_2111\|m.2065 type:complete len:535 (+) asmbl_2111:397-2001(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_2115\|g.1373 ORF ID=asmbl_2115\|g.1373 ID=asmbl_2115\|m.1373 type:complete len:535 (+) ID=asmbl_2115:397-2001(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_2211\|g.1358 ORF ID=asmbl_2211\|g.1358 ID=asmbl_2211\|m.1358 type:complete len:535 (+) ID=asmbl_2211:393-1997(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	534 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA ] cytoplasm [IDA] mRNA cleavage factor complex [IPI]
Biological Process:	mRNA polyadenylation [IDA][IMP] mRNA cleavage [IDA]
Molecular Function:	RNA binding [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSSDEEDFND IYGDDKPTTT EEVKKEEEQN KAGSGTSQLD QLAALQALSS SLNKLNNPNS  60
   61 NNSSSNNSNQ DTSSSKQDGT ANDKEGSNED TKNEKKQESA TSANANANAS SAGPSGLPWE 120
  121 QLQQTMSQFQ QPSSQSPPQQ QVTQTKEERS KADLSKESCK MFIGGLNWDT TEDNLREYFG 180
  181 KYGTVTDLKI MKDPATGRSR GFGFLSFEKP SSVDEVVKTQ HILDGKVIDP KRAIPRDEQD 240
  241 KTGKIFVGGI GPDVRPKEFE EFFSQWGTII DAQLMLDKDT GQSRGFGFVT YDSADAVDRV 300
  301 CQNKFIDFKD RKIEIKRAEP RHMQQKSSNN GGNNGGNNMN RRGGNFGNQG DFNQMYQNPM 360
  361 MGGYNPMMNP QAMTDYYQKM QEYYQQMQKQ TGMDYTQMYQ QQMQQMAMMM PGFAMPPNAM 420
  421 TLNQPQQDSN ATQGSPAPSD SDNNKSNDVQ TIGNTSNTDS GSPPLNLPNG PKGPSQYNDD 480
  481 HNSGYGYNRD RGDRDRNDRD RDYNHRSGGN HRRNGRGGRG GYNRRNNGYH PYNR

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ENP1 HRP1 KAP104 NAB2 YNL035C
View Details	(MIPS) Mewes HW, et al. (2004)		CLP1 HRP1 PCF11 RNA14 RNA15
View Details	Gavin AC, et al. (2006)		AEP1 HRP1 KAP104 RPS9A
View Details	Gavin AC, et al. (2002)		AEP1 AEP2 ATP22 BMH1 BMH2 CLU1 CRM1 ECM29 FKS1 GCN1 GEA2 GFA1 HRP1 KAP104 KAP123 LCB1 LCB2 LYS12 MIR1 NAB2 SAM1 SAM2 SES1 TSR1 YHR020W
View Details	Ho Y, et al. (2002)		ADH4 ADR1 ARC1 AYR1 BGL2 CBP6 DIM1 DPS1 ENP1 FAA1 GAR1 GSP1 GSP2 HEM15 HRP1 HTA2 KAP104 KTR3 MRT4 NAB2 NDE1 OAC1 PAA1 PET10 PET9 PGM2 PSD1 RPT4 RTN2 SAC1 TFG2 VMA8 YDL063C YHM2 YNL035C YRB1
View Details	Qiu et al. (2008)		CFT1 CFT2 CLP1 FIP1 HRP1 PAP1 PFS2 PTA1 PTI1 REF2 RNA14 RNA15 SSU72 SWD2 YSH1 YTH1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	STE50	HRP1	2	Unpublished Fields Lab Data
View Screen	YDR049W	HRP1	2	Unpublished Fields Lab Data
View Screen	MPE1	HRP1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	HRP1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..58]	deduced	N/A	No confident structure predictions are available.
2	View Details	[59..152]	PSI-BLAST	167.39794	Poly(A)-binding protein
3	View Details	[153..244]	PSI-BLAST	402.9794	Nuclear ribonucleoprotein A1 (RNP A1, UP1)
4	View Details	[245..347]	PSI-BLAST	13.0	Polypyrimidine tract-binding protein
5	View Details	[348..429]	PSI-BLAST	13.0	Polypyrimidine tract-binding protein
6	View Details	[430..534]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
RNA binding	6.73097473547076	bayes_pls_golite062009
mRNA binding	5.29759606920196	bayes_pls_golite062009
nucleic acid binding	4.51665560478175	bayes_pls_golite062009
	4.45006565905155	bayes_pls_golite062009
binding	3.64400841800969	bayes_pls_golite062009
single-stranded RNA binding	2.93798024346416	bayes_pls_golite062009
poly-pyrimidine tract binding	2.79052809544187	bayes_pls_golite062009
structural molecule activity	2.78501986457503	bayes_pls_golite062009
transcription regulator activity	1.7776932219095	bayes_pls_golite062009
DNA binding	1.65208390030564	bayes_pls_golite062009
snRNA binding	1.44241647066188	bayes_pls_golite062009
protein binding	1.12215421354121	bayes_pls_golite062009
mRNA 3'-UTR binding	0.824614388276642	bayes_pls_golite062009
transcription factor activity	0.795084003256395	bayes_pls_golite062009
translation regulator activity	0.38120065819101	bayes_pls_golite062009
sequence-specific DNA binding	0.304235325881492	bayes_pls_golite062009
U1 snRNA binding	0.290135317640781	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.128616879457381	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
RNA binding	6.73097473547076	bayes_pls_golite062009
mRNA binding	5.29759606920196	bayes_pls_golite062009
nucleic acid binding	4.51665560478175	bayes_pls_golite062009
	4.45006565905155	bayes_pls_golite062009
binding	3.64400841800969	bayes_pls_golite062009
single-stranded RNA binding	2.93798024346416	bayes_pls_golite062009
poly-pyrimidine tract binding	2.79052809544187	bayes_pls_golite062009
structural molecule activity	2.78501986457503	bayes_pls_golite062009
transcription regulator activity	1.7776932219095	bayes_pls_golite062009
DNA binding	1.65208390030564	bayes_pls_golite062009
snRNA binding	1.44241647066188	bayes_pls_golite062009
protein binding	1.12215421354121	bayes_pls_golite062009
mRNA 3'-UTR binding	0.824614388276642	bayes_pls_golite062009
transcription factor activity	0.795084003256395	bayes_pls_golite062009
translation regulator activity	0.38120065819101	bayes_pls_golite062009
sequence-specific DNA binding	0.304235325881492	bayes_pls_golite062009
U1 snRNA binding	0.290135317640781	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.128616879457381	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2