Protein Overview: CBK1

General Information:
Name(s) found:	CBK1 / YNL161W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324168\|ref\|NP_014238.1\| Serine/threonine protein kinase that regulates cell morphogenesis pathways; involved in cell wall biosynthesis, apical growth, proper mating projection morphology, bipolar bud site selection in diploid cells, and cell separatio [NCBI NR] pir\|\|S60966 probable protein kinase YNL161w (EC 2.7.1.-) - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1730060\|sp\|P53894.1\|CBK1_YEAST RecName: Full=Serine/threonine-protein kinase CBK1; AltName: Full=Cell wall biosynthesis kinase [NCBI NR] gi\|1302128\|emb\|CAA96048.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|1050791\|emb\|CAA63278.1\| N1727 [Saccharomyces cerevisiae] [NCBI NR] Serine/threonine-protein kinase CBK1 OS=Saccharomyces cerevisiae GN=CBK1 PE=1 SV=1 [Swiss-Prot] CBK1 Protein kinase involved in cell wall biosynthesis [mips] Serine/threonine protein kinase that regulates cell morphogenesis pathways; involved in cell wall biosynthesis, apical growth, proper mating projection morphology, bipolar bud site selection in diploid cells, and cell separation [SGD] CBK1, Chr XIV from 332594-334864 [yeast_orfs2] Chr XIV from 332594-334864 [yeastorf3.fasta] (P53894) Serine/threonine-protein kinase CBK1 (EC 2.7.1.-) (Cell wall biosynthesis kinase) [4932.FASTACCERV.txt] CBK1 SGDID:S0005105, Chr XIV from 332597-334867, Verified ORF [yeastflysequest.fasta] Cbk1p [Saccharomyces cerevisiae]gi\|1050791\|emb\|CAA63278.1\| N1727 [Saccharomyces cerevisiae]gi\|1302128\|emb\|CAA96048.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|2133047\|pir\|\|S60966 probable protein kinase YNL161w (EC 2.7.1.-) - yeast (Saccha [nrdb_11152004_Chlamydomonas] (P53894) Serine/threonine-protein kinase CBK1 (EC 2.7.1.37) (Cell wall biosynthesis kinase) [cerv4932.FASTAC] CBK1 SGDID:S000005105, Chr XIV from 332599-334869, Verified ORF, "Serine/threonine protein kinase that regulates cell morphogenesis pathways; involved in cell wall biosynthesis, apical growth, proper mating projection morphology, bipolar bud site selectio [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] N1727 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Serine/threonine-protein kinase CBK1 (Cell wall biosynthesis kinase) [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Serine/threonine protein kinase that regulates cell morphogenesis pathways; involved in cell wall biosynthesis, apical growth, proper mating projection morphology, bipolar bud site selection in diploid cells, and cell separation; Cbk1p [Saccharomyces cere [nr-271106-contam.fasta] CBK1 SGDID:S000005105, Chr XIV from 332599-334869, Verified ORF, "Serine/threonine protein kinase that regulates cell morphogenesis pathways; involved in cell wall biosynthesis, apical growth, proper mating projection morphology, bipolar bud site selection in diploid cells, and cell separation" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Serine/threonine-protein kinase CBK1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CBK1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] CBK1 SGDID:S000005105, Chr XIV from 332597-334867, Genome Release 64-1-1, Verified ORF, "Serine/threonine protein kinase that regulates cell morphogenesis pathways, including cell wall biosynthesis, mating projection morphology, bipolar bud site selection; regulates SRL1 mRNA localization via phosphorylation of substrate Ssd1p" [yeast-030211-contam.fasta] CBK1 SGDID:S000005105, Chr XIV from 332597-334867, Genome Release 64-2-1, Verified ORF, "Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress" [20141208_orf_trans.fasta] YNL161W SGDID:S000005105, Chr XIV from 332597-334867, Genome Release 64-1-1, Verified ORF, "Serine/threonine protein kinase that regulates cell morphogenesis pathways, including cell wall biosynthesis, mating projection morphology, bipolar bud site selection; regulates SRL1 mRNA localization via phosphorylation of substrate Ssd1p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	756 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] cellular bud [IDA] cytoplasm [IDA] cellular bud neck [IDA] mating projection tip [TAS]
Biological Process:	response to pheromone involved in conjugation with cellular fusion [IDA] cell morphogenesis during vegetative growth [IGI] regulation of exit from mitosis [IMP] cell morphogenesis involved in conjugation with cellular fusion [IDA]
Molecular Function:	protein kinase activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MYNSSTNHHE GAPTSGHGYY MSQQQDQQHQ QQQQYANEMN PYQQIPRPPA AGFSSNYMKE  60
   61 QGSHQSLQEH LQRETGNLGS GFTDVPALNY PATPPPHNNY AASNQMINTP PPSMGGLYRH 120
  121 NNNSQSMVQN GNGSGNAQLP QLSPGQYSIE SEYNQNLNGS SSSSPFHQPQ TLRSNGSYSS 180
  181 GLRSVKSFQR LQQEQENVQV QQQLSQAQQQ NSRQQQQQLQ YQQQQQQQQQ QQHMQIQQQQ 240
  241 QQQQQQQQSQ SPVQSGFNNG TISNYMYFER RPDLLTKGTQ DKAAAVKLKI ENFYQSSVKY 300
  301 AIERNERRVE LETELTSHNW SEERKSRQLS SLGKKESQFL RLRRTRLSLE DFHTVKVIGK 360
  361 GAFGEVRLVQ KKDTGKIYAM KTLLKSEMYK KDQLAHVKAE RDVLAGSDSP WVVSLYYSFQ 420
  421 DAQYLYLIME FLPGGDLMTM LIRWQLFTED VTRFYMAECI LAIETIHKLG FIHRDIKPDN 480
  481 ILIDIRGHIK LSDFGLSTGF HKTHDSNYYK KLLQQDEATN GISKPGTYNA NTTDTANKRQ 540
  541 TMVVDSISLT MSNRQQIQTW RKSRRLMAYS TVGTPDYIAP EIFLYQGYGQ ECDWWSLGAI 600
  601 MYECLIGWPP FCSETPQETY RKIMNFEQTL QFPDDIHISY EAEDLIRRLL THADQRLGRH 660
  661 GGADEIKSHP FFRGVDWNTI RQVEAPYIPK LSSITDTRFF PTDELENVPD SPAMAQAAKQ 720
  721 REQMTKQGGS APVKEDLPFI GYTYSRFDYL TRKNAL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Ho Y, et al. (2002)		ARP2 CBK1 ECM10 GAL7 MOB2 PRB1 SEC28 SGT2 SIS1 SSD1 TAO3 UBP15 VMA6
View Details	Qiu et al. (2008)		CBK1 HBT1 KIC1 MOB2 SOG2 STE20 STE4

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	TRZ1	No Comments	Hazbun TR, et al. (2003)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	None Entered	#17 Mitotic Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RAD6	CBK1	1	Unpublished Fields Lab Data
View Screen	MPE1	CBK1	1	Unpublished Fields Lab Data
View Screen	YJU2	CBK1	1	Unpublished Fields Lab Data
View Screen	FAR10	CBK1	1	Unpublished Fields Lab Data
View Screen	PLB1	CBK1	1	Unpublished Fields Lab Data
View Screen	RRB1	CBK1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	CBK1	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..323]	deduced	N/A	No confident structure predictions are available.
2	View Details	[324..503]	PSI-BLAST	94.0	No description for 1lhxA_ was found.
3	View Details	[504..636]	deduced	N/A	No confident structure predictions are available.
4	View Details	[637..756]	PSI-BLAST	440.228787	cAMP-dependent PK, catalytic subunit

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
phosphotransferase activity, alcohol group as acceptor	5.49988294224815	bayes_pls_golite062009
protein kinase activity	5.4586557943719	bayes_pls_golite062009
kinase activity	5.3287080791198	bayes_pls_golite062009
calmodulin-dependent protein kinase activity	5.10630503791738	bayes_pls_golite062009
cyclic nucleotide-dependent protein kinase activity	4.97855712164511	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	4.96052051118534	bayes_pls_golite062009
cAMP-dependent protein kinase activity	4.66277812893755	bayes_pls_golite062009
protein serine/threonine kinase activity	4.46381887831481	bayes_pls_golite062009
phosphorylase kinase activity	4.23570595363447	bayes_pls_golite062009
transferase activity	3.97138602499442	bayes_pls_golite062009
actin binding	2.91722352767433	bayes_pls_golite062009
protein tyrosine kinase activity	2.8153155526437	bayes_pls_golite062009
binding	2.74863538127007	bayes_pls_golite062009
structural molecule activity	2.60280308499348	bayes_pls_golite062009
transmembrane receptor activity	2.59526003830791	bayes_pls_golite062009
transmembrane receptor protein kinase activity	2.54265774272269	bayes_pls_golite062009
ion transmembrane transporter activity	2.52531786618214	bayes_pls_golite062009
cation transmembrane transporter activity	2.46424348876434	bayes_pls_golite062009
molecular transducer activity	2.40586892801446	bayes_pls_golite062009
signal transducer activity	2.40586892801446	bayes_pls_golite062009
receptor activity	2.39311927684508	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	2.37122853419919	bayes_pls_golite062009
protein binding	2.276111749106	bayes_pls_golite062009
tubulin binding	2.18641675163967	bayes_pls_golite062009
transmembrane receptor protein tyrosine kinase activity	2.16831387024372	bayes_pls_golite062009
cytoskeletal protein binding	2.12615078788254	bayes_pls_golite062009
receptor signaling protein activity	2.10379226653669	bayes_pls_golite062009
ATP binding	1.97600611746091	bayes_pls_golite062009
adenyl ribonucleotide binding	1.93596671856664	bayes_pls_golite062009
adenyl nucleotide binding	1.90928021591891	bayes_pls_golite062009
metal ion transmembrane transporter activity	1.9016235206634	bayes_pls_golite062009
microtubule binding	1.88110801192607	bayes_pls_golite062009
receptor signaling protein serine/threonine kinase activity	1.81249386965144	bayes_pls_golite062009
molecular adaptor activity	1.81145713371725	bayes_pls_golite062009
actin filament binding	1.75933904320288	bayes_pls_golite062009
purine ribonucleotide binding	1.65732943334212	bayes_pls_golite062009
ribonucleotide binding	1.65726906484847	bayes_pls_golite062009
purine nucleotide binding	1.63763394711025	bayes_pls_golite062009
nucleotide binding	1.63195828048121	bayes_pls_golite062009
insulin receptor substrate binding	1.62657794525303	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	1.6084489855403	bayes_pls_golite062009
SH3/SH2 adaptor activity	1.59474164216385	bayes_pls_golite062009
transcription regulator activity	1.56748951434625	bayes_pls_golite062009
JUN kinase kinase kinase kinase activity	1.55257528159541	bayes_pls_golite062009
DNA binding	1.35333135345112	bayes_pls_golite062009
gamma-tubulin binding	1.28511591054866	bayes_pls_golite062009
kinase binding	1.26086833316556	bayes_pls_golite062009
phosphoinositide 3-kinase binding	1.25285260301349	bayes_pls_golite062009
catalytic activity	1.21655087018101	bayes_pls_golite062009
nucleic acid binding	1.16049201286103	bayes_pls_golite062009
transporter activity	1.11833481501707	bayes_pls_golite062009
transcription factor activity	1.10928254946881	bayes_pls_golite062009
protein kinase binding	1.06030596516506	bayes_pls_golite062009
cation channel activity	1.04999722123563	bayes_pls_golite062009
telethonin binding	1.04264263421201	bayes_pls_golite062009
transmembrane transporter activity	0.97575992765505	bayes_pls_golite062009
cyclin-dependent protein kinase activity	0.959360414036299	bayes_pls_golite062009
growth factor binding	0.86076618871384	bayes_pls_golite062009
protein binding, bridging	0.83823250804321	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	0.835012681606991	bayes_pls_golite062009
ligase activity, forming aminoacyl-tRNA and related compounds	0.835012681606991	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	0.832111648073981	bayes_pls_golite062009
MAP kinase activity	0.81943579965027	bayes_pls_golite062009
structural constituent of muscle	0.767275562047313	bayes_pls_golite062009
substrate-specific transporter activity	0.705905455086486	bayes_pls_golite062009
phosphoinositide 3-kinase regulator activity	0.688047400629641	bayes_pls_golite062009
vinculin binding	0.666656406096231	bayes_pls_golite062009
ligase activity	0.665445021164512	bayes_pls_golite062009
structural constituent of cytoskeleton	0.612883428421652	bayes_pls_golite062009
acetylcholine receptor binding	0.594548112804154	bayes_pls_golite062009
transcription repressor activity	0.568332175782799	bayes_pls_golite062009
protein serine/threonine/tyrosine kinase activity	0.48345772948903	bayes_pls_golite062009
enzyme binding	0.46284745266176	bayes_pls_golite062009
magnesium ion binding	0.393328812780268	bayes_pls_golite062009
MAP kinase kinase kinase activity	0.37115026509016	bayes_pls_golite062009
MAP kinase kinase activity	0.35402045013148	bayes_pls_golite062009
phosphotyrosine binding	0.311079584914389	bayes_pls_golite062009
passive transmembrane transporter activity	0.287189255116606	bayes_pls_golite062009
channel activity	0.287189255116606	bayes_pls_golite062009
structure-specific DNA binding	0.267404531445803	bayes_pls_golite062009
insulin receptor binding	0.266450215230107	bayes_pls_golite062009
substrate-specific channel activity	0.261869836686301	bayes_pls_golite062009
myosin binding	0.259936573810332	bayes_pls_golite062009
RNA binding	0.245748631571068	bayes_pls_golite062009
ion channel activity	0.241519346930618	bayes_pls_golite062009
protein complex binding	0.239339759110451	bayes_pls_golite062009
insulin receptor activity	0.237134032032089	bayes_pls_golite062009
SH2 domain binding	0.226760162354251	bayes_pls_golite062009
transmembrane receptor protein serine/threonine kinase activity	0.20643152368029	bayes_pls_golite062009
transforming growth factor beta receptor activity	0.20643152368029	bayes_pls_golite062009
alpha-actinin binding	0.181804774839734	bayes_pls_golite062009
protein kinase C activity	0.0795689006739899	bayes_pls_golite062009
transforming growth factor beta receptor activity, type I	0.0506751594607393	bayes_pls_golite062009
actin-dependent ATPase activity	0.0339299940546054	bayes_pls_golite062009
MAP kinase kinase kinase kinase activity	0.01826424351081	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.0040394359628233	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2