Protein Overview: MCM7

General Information:
Name(s) found:	MCM7 / YBR202W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6319679\|ref\|NP_009761.1\| Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-ph [NCBI NR] gi\|55977791\|sp\|P38132.3\|CDC47_YEAST RecName: Full=DNA replication licensing factor CDC47; AltName: Full=Cell division control protein 47 [NCBI NR] gi\|536577\|emb\|CAA85166.1\| CDC47 [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S34027 replication licensing factor MCM7 [validated] - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|311678\|emb\|CAA79689.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] DNA replication licensing factor CDC47 OS=Saccharomyces cerevisiae GN=CDC47 PE=1 SV=3 [Swiss-Prot] CDC47 Cell division control protein [mips] Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase [SGD] CDC47, Chr II from 625730-628267 [yeast_orfs2] Chr II from 625730-628267 [yeastorf3.fasta] Chr II from 625729-628266 [yeastorf4.fasta] Chr II from 625727-628264 [yeastorf5.fasta] CDC47 SGDID:S0000406, Chr II from 625729-628266, Verified ORF [yeastflysequest.fasta] CDC47 SGDID:S0000406, Chr II from 625767-628304, Verified ORF [10112005cervSGD.fasta] Cdc47p [Saccharomyces cerevisiae]gi\|311678\|emb\|CAA79689.1\| unknown [Saccharomyces cerevisiae]gi\|536577\|emb\|CAA85166.1\| CDC47 [Saccharomyces cerevisiae]gi\|419857\|pir\|\|S34027 replication licensing factor MCM7 [validated] - yeast (Saccharomyces cerevisia [nrdb_11152004_Chlamydomonas] CDC47 SGDID:S000000406, Chr II from 625767-628304, Verified ORF, "Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] MCM7 SGDID:S000000406, Chr II from 625767-628304, Verified ORF, "Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] CDC47 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase; Cdc47p [Saccharomyces c [nr-271106-contam.fasta] DNA replication licensing factor CDC47 (Cell division control protein 47) [nr-271106-contam.fasta] unknown [Saccharomyces cerevisiae] [nr-271106-contam.fasta] MCM7 SGDID:S000000406, Chr II from 625767-628304, Verified ORF, "Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase" [yeast-bovrabprots.fasta] CDC47 SGDID:S000000406, Chr II from 625767-628304, Verified ORF, "Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase" [yeast-contam_custom.fasta] [chemostatdb.fasta]
Organism:	Saccharomyces cerevisiae
Length:	845 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] pre-replicative complex [IDA] cytoplasm [IDA][TAS] MCM complex [IDA]
Biological Process:	DNA replication initiation [TAS] pre-replicative complex assembly [IPI] DNA unwinding involved in replication [TAS]
Molecular Function:	ATP binding [IDA] chromatin binding [TAS] ATP-dependent DNA helicase activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSAALPSIQL PVDYNNLFNE ITDFLVTFKQ DTLSSDATRN ENEDENLDAE NIEQHLLEKG  60
   61 PKYMAMLQKV ANRELNSVII DLDDILQYQN EKFLQGTQAD DLVSAIQQNA NHFTELFCRA 120
  121 IDNNMPLPTK EIDYKDDVLD VILNQRRLRN ERMLSDRTNE IRSENLMDTT MDPPSSMNDA 180
  181 LREVVEDETE LFPPNLTRRY FLYFKPLSQN CARRYRKKAI SSKPLSVRQI KGDFLGQLIT 240
  241 VRGIITRVSD VKPAVEVIAY TCDQCGYEVF QEVNSRTFTP LSECTSEECS QNQTKGQLFM 300
  301 STRASKFSAF QECKIQELSQ QVPVGHIPRS LNIHVNGTLV RSLSPGDIVD VTGIFLPAPY 360
  361 TGFKALKAGL LTETYLEAQF VRQHKKKFAS FSLTSDVEER VMELITSGDV YNRLAKSIAP 420
  421 EIYGNLDVKK ALLLLLVGGV DKRVGDGMKI RGDINVCLMG DPGVAKSQLL KAICKISPRG 480
  481 VYTTGKGSSG VGLTAAVMKD PVTDEMILEG GALVLADNGI CCIDEFDKMD ESDRTAIHEV 540
  541 MEQQTISISK AVINTNPGAR TSILAAANPL YGRINPRLSP LDNINLPAAL LSRFDILFLM 600
  601 LDIPSRDDDE KLAEHVTYVH MHNKQPDLDF TPVEPSKMRE YIAYAKTKRP VMSEAVNDYV 660
  661 VQAYIRLRQD SKREMDSKFS FGQATPRTLL GIIRLSQALA KLRLADMVDI DDVEEALRLV 720
  721 RVSKESLYQE TNKSKEDESP TTKIFTIIKK MLQETGKNTL SYENIVKTVR LRGFTMLQLS 780
  781 NCIQEYSYLN VWHLINEGNT LKFVDDGTMD TDQEDSLVST PKLAPQTTAS ANVSAQDSDI 840
  841 DLQDA

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		CDC45 CDC6 DPB11 DPB2 DPB3 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 POL2 POL3 POL31 POL32 SLD3
View Details	Gavin AC, et al. (2006)		ERG26 MCM7 MYO5
View Details	Gavin AC, et al. (2002)		CDC45 ERG26 GLT1 HAT1 HSP42 LYS12 MCM2 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 RPD3 RPN9 SIN3 TFP1
View Details	Ho Y, et al. (2002)		ADR1 ARC15 ARC18 ARC19 ARC35 ARC40 ARP2 ARP3 BNI1 DUR1,2 IMH1 KAP122 MCM4 MCM7 MET18 RPT4 TRP3 YJR029W
View Details	Qiu et al. (2008)		CDC45 CDC6 DPB11 DPB2 DPB3 HCS1 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 POL2 POL3 POL31 POL32 RFC1 RFC4 RRM3 SLD3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#13 Mitotic Prep2-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..206]	MSA	1.089991	View MSA. No confident structure predictions are available.
2	View Details	[207..417]	Pfam	8.79588	MCM2/3/5 family No confident structure predictions are available.
3	View Details	[418..646]	PSI-BLAST	46.522879	ATPase subunit of magnesium chelatase, BchI
4	View Details	[647..760]	PSI-BLAST	46.522879	ATPase subunit of magnesium chelatase, BchI
5	View Details	[761..845]	MSA	2.270992	View MSA. No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
nucleic acid binding	4.29626483284963	bayes_pls_golite062009
DNA binding	3.71771169141041	bayes_pls_golite062009
structure-specific DNA binding	3.48139490984491	bayes_pls_golite062009
single-stranded DNA binding	3.43027351695641	bayes_pls_golite062009
binding	2.83465364476231	bayes_pls_golite062009
telomerase inhibitor activity	2.77979101622935	bayes_pls_golite062009
structural constituent of ribosome	2.13063800565247	bayes_pls_golite062009
transcription regulator activity	2.10110937001387	bayes_pls_golite062009
structural molecule activity	1.9177190829469	bayes_pls_golite062009
DNA replication origin binding	1.84819558112187	bayes_pls_golite062009
telomeric DNA binding	1.7847835613194	bayes_pls_golite062009
3'-5'-exoribonuclease activity	1.54401231841266	bayes_pls_golite062009
single-stranded telomeric DNA binding	1.48033977789232	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	1.35850109411274	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	1.35845277283398	bayes_pls_golite062009
ligase activity, forming aminoacyl-tRNA and related compounds	1.35845277283398	bayes_pls_golite062009
transcription repressor activity	1.30343463892105	bayes_pls_golite062009
DNA-directed RNA polymerase activity	1.19766815287926	bayes_pls_golite062009
RNA polymerase activity	1.19766815287926	bayes_pls_golite062009
ribonuclease activity	1.19236946227657	bayes_pls_golite062009
transcription factor activity	1.14242112812892	bayes_pls_golite062009
DNA helicase activity	1.12203697857261	bayes_pls_golite062009
sequence-specific DNA binding	0.85691075793641	bayes_pls_golite062009
DNA-dependent ATPase activity	0.638831894247026	bayes_pls_golite062009
RNA polymerase II transcription factor activity	0.583085644017496	bayes_pls_golite062009
nuclease activity	0.57381531077345	bayes_pls_golite062009
ligase activity	0.346041081217685	bayes_pls_golite062009
nucleotidyltransferase activity	0.325774164899685	bayes_pls_golite062009
double-stranded DNA binding	0.314881439089948	bayes_pls_golite062009
translation regulator activity	0.31332179848255	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.311535572386606	bayes_pls_golite062009
exonuclease activity	0.28313884527829	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.263197317040851	bayes_pls_golite062009
translation initiation factor activity	0.2080607201312	bayes_pls_golite062009
chromatin binding	0.19409870825073	bayes_pls_golite062009
RNA binding	0.18502033889261	bayes_pls_golite062009
protein binding	0.152645195799531	bayes_pls_golite062009
aspartate-tRNA ligase activity	0.151650064901878	bayes_pls_golite062009
exoribonuclease activity, producing 5'-phosphomonoesters	0.125256208482441	bayes_pls_golite062009
exoribonuclease activity	0.109774918833391	bayes_pls_golite062009
exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	0.0954216620147701	bayes_pls_golite062009
ATP-dependent DNA helicase activity	0.0179535526984695	bayes_pls_golite062009
helicase activity	0.0121541775159524	bayes_pls_golite062009

Term	Confidence	Notes
DNA clamp loader activity	4.91429252297969	bayes_pls_golite062009
protein-DNA loading ATPase activity	4.80695929076807	bayes_pls_golite062009
DNA-dependent ATPase activity	2.72462789867829	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	2.66107861968804	bayes_pls_golite062009
binding	2.64537357529343	bayes_pls_golite062009
nucleic acid binding	2.51683451033161	bayes_pls_golite062009
DNA helicase activity	2.2817164193758	bayes_pls_golite062009
pyrophosphatase activity	2.13284212711072	bayes_pls_golite062009
DNA binding	2.12258000781149	bayes_pls_golite062009
transcription regulator activity	2.09345198747025	bayes_pls_golite062009
ATP-dependent DNA helicase activity	1.88455339138556	bayes_pls_golite062009
helicase activity	1.70242731830224	bayes_pls_golite062009
RNA helicase activity	1.63960194106079	bayes_pls_golite062009
DNA replication origin binding	1.62491387774159	bayes_pls_golite062009
ATP-dependent RNA helicase activity	1.61735400124552	bayes_pls_golite062009
RNA-dependent ATPase activity	1.60404584546501	bayes_pls_golite062009
ribonucleotide binding	1.53142082854758	bayes_pls_golite062009
purine nucleotide binding	1.52938239070386	bayes_pls_golite062009
purine ribonucleotide binding	1.52887308780164	bayes_pls_golite062009
nucleotide binding	1.52746968836881	bayes_pls_golite062009
microtubule motor activity	1.35938517416356	bayes_pls_golite062009
ATP binding	1.23942044024113	bayes_pls_golite062009
adenyl ribonucleotide binding	1.21044170258803	bayes_pls_golite062009
structure-specific DNA binding	1.20315703345493	bayes_pls_golite062009
adenyl nucleotide binding	1.19481945569892	bayes_pls_golite062009
ATP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
purine NTP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
double-stranded DNA binding	1.05408274676326	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
DNA-directed DNA polymerase activity	0.971476020912368	bayes_pls_golite062009
DNA polymerase activity	0.928091843881235	bayes_pls_golite062009
transcription factor activity	0.887108427603557	bayes_pls_golite062009
transcription repressor activity	0.862685397469217	bayes_pls_golite062009
hydrogen ion transporting ATP synthase activity, rotational mechanism	0.8469248456592	bayes_pls_golite062009
kinase activity	0.641541011250583	bayes_pls_golite062009
motor activity	0.61636596887462	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.584453361662212	bayes_pls_golite062009
single-stranded DNA binding	0.406600732518248	bayes_pls_golite062009
protein kinase activity	0.351308564667977	bayes_pls_golite062009
RNA polymerase II transcription factor activity	0.243902356228423	bayes_pls_golite062009
protein binding	0.170837278876362	bayes_pls_golite062009
hydrogen ion transmembrane transporter activity	0.156278588350022	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.147579541298736	bayes_pls_golite062009
mismatched DNA binding	0.13366911641814	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.113504049290503	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.111293884322157	bayes_pls_golite062009
structural constituent of ribosome	0.089234257580272	bayes_pls_golite062009
3'-5' DNA helicase activity	0.0769112340058804	bayes_pls_golite062009
histone methyltransferase activity (H3-K9 specific)	0.0642842984371965	bayes_pls_golite062009
cytoskeletal protein binding	0.0272673919333979	bayes_pls_golite062009
monovalent inorganic cation transmembrane transporter activity	0.0223627159838333	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

	PROTEIN(S)	PUBLICATION
View Data	MCM7	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Protein Overview: MCM7

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: