Protein Overview: MOT1

General Information:
Name(s) found:	MOT1 / YPL082C [SGD]
Description(s) found: Found 26 descriptions. SHOW ALL	Essential abundant protein involved in regulation of transcription, removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity [SGD]
Organism:	Saccharomyces cerevisiae
Length:	1867 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA ] nuclear chromosome [IDA] nuclear outer membrane [IMP]
Biological Process:	transcription of nuclear rRNA large RNA polymerase I transcript [IDA][IMP] regulation of RNA polymerase II transcriptional preinitiation complex assembly [IMP] regulation of transcription from RNA polymerase II promoter [IDA]
Molecular Function:	ATPase activity [IDA] rDNA binding [IDA] TATA-binding protein binding [IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTSRVSRLDR QVILIETGST QVVRNMAADQ MGDLAKQHPE DILSLLSRVY PFLLVKKWET  60
   61 RVTAARAVGG IVAHAPSWDP NESDLVGGTN EGSPLDNAQV KLEHEMKIKL EEATQNNQLN 120
  121 LLQEDHHLSS LSDWKLNEIL KSGKVLLASS MNDYNVLGKA DDNIRKQAKT DDIKQETSML 180
  181 NASDKANENK SNANKKSARM LAMARRKKKM SAKNTPKHPV DITESSVSKT LLNGKNMTNS 240
  241 AASLATSPTS NQLNPKLEIT EQADESKLMI ESTVRPLLEQ HEIVAGLVWQ FQGIYELLLD 300
  301 NLMSENWEIR HGAALGLREL VKKHAYGVSR VKGNTREENN LRNSRSLEDL ASRLLTVFAL 360
  361 DRFGDYVYDT VVAPVRESVA QTLAALLIHL DSTLSIKIFN CLEQLVLQDP LQTGLPNKIW 420
  421 EATHGGLLGI RYFVSIKTNF LFAHGLLENV VRIVLYGLNQ SDDDVQSVAA SILTPITSEF 480
  481 VKLNNSTIEI LVTTIWSLLA RLDDDISSSV GSIMDLLAKL CDHQEVLDIL KNKALEHPSE 540
  541 WSFKSLVPKL YPFLRHSISS VRRAVLNLLI AFLSIKDDST KNWLNGKVFR LVFQNILLEQ 600
  601 NPELLQLSFD VYVALLEHYK VKHTEKTLDH VFSKHLQPIL HLLNTPVGEK GKNYAMESQY 660
  661 ILKPSQHYQL HPEKKRSISE TTTDSDIPIP KNNEHINIDA PMIAGDITLL GLDVILNTRI 720
  721 MGAKAFALTL SMFQDSTLQS FFTNVLVRCL ELPFSTPRML AGIIVSQFCS SWLQKHPEGE 780
  781 KLPSFVSEIF SPVMNKQLLN RDEFPVFREL VPSLKALRTQ CQSLLATFVD VGMLPQYKLP 840
  841 NVAIVVQGET EAGPHAFGVE TAEKVYGEYY DKMFKSMNNS YKLLAKKPLE DSKHRVLMAI 900
  901 NSAKESAKLR TGSILANYAS SILLFDGLPL KLNPIIRSLM DSVKEERNEK LQTMAGESVV 960
  961 HLIQQLLENN KVNVSGKIVK NLCGFLCVDT SEVPDFSVNA EYKEKILTLI KESNSIAAQD1020
 1021 DINLAKMSEE AQLKRKGGLI TLKILFEVLG PSILQKLPQL RSILFDSLSD HENEEASKVD1080
 1081 NEQGQKIVDS FGVLRALFPF MSDSLRSSEV FTRFPVLLTF LRSNLSVFRY SAARTFADLA1140
 1141 KISSVEVMAY TIREILPLMN SAGSLSDRQG STELIYHLSL SMETDVLPYV IFLIVPLLGR1200
 1201 MSDSNEDVRN LATTTFASII KLVPLEAGIA DPKGLPEELV ASRERERDFI QQMMDPSKAK1260
 1261 PFKLPIAIKA TLRKYQQDGV NWLAFLNKYH LHGILCDDMG LGKTLQTICI IASDQYLRKE1320
 1321 DYEKTRSVES RALPSLIICP PSLTGHWENE FDQYAPFLKV VVYAGGPTVR LTLRPQLSDA1380
 1381 DIIVTSYDVA RNDLAVLNKT EYNYCVLDEG HIIKNSQSKL AKAVKEITAN HRLILTGTPI1440
 1441 QNNVLELWSL FDFLMPGFLG TEKMFQERFA KPIAASRNSK TSSKEQEAGV LALEALHKQV1500
 1501 LPFMLRRLKE DVLSDLPPKI IQDYYCELGD LQKQLYMDFT KKQKNVVEKD IENSEIADGK1560
 1561 QHIFQALQYM RKLCNHPALV LSPNHPQLAQ VQDYLKQTGL DLHDIINAPK LSALRTLLFE1620
 1621 CGIGEEDIDK KASQDQNFPI QNVISQHRAL IFCQLKDMLD MVENDLFKKY MPSVTYMRLD1680
 1681 GSIDPRDRQK VVRKFNEDPS IDCLLLTTKV GGLGLNLTGA DTVIFVEHDW NPMNDLQAMD1740
 1741 RAHRIGQKKV VNVYRIITKG TLEEKIMGLQ KFKMNIASTV VNQQNSGLAS MDTHQLLDLF1800
 1801 DPDNVTSQDN EEKNNGDSQA AKGMEDIANE TGLTGKAKEA LGELKELWDP SQYEEEYNLD1860
 1861 TFIKTLR

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		HHF1, HHF2 HTA2 HTB1 IOC3 ISW1 ISW2 ITC1 MOT1 NPL6 RPO31 RSC2 RSC3 RSC4 RSC58 RSC8 SPT15 STH1 TAF5 VPS1
View Details	Krogan NJ, et al. (2006)		CSI1 CSN9 ERV46 FPR2 HUL5 IQG1 MOT1 MPT5 NAS6 NMD3 NPT1 PCI8 PEP1 PST1 PUS9 RPN1 RPN10 RPN11 RPN12 RPN13 RPN14 RPN2 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 RRI1 RRI2 SEC18 SEM1 SKP1 SPG5 SPO77 SQT1 SWA2 TCB2 TIP41 TOD6 TOR2 TSR4 UBP6 YEN1 YKL027W YMR258C YNL022C YPR003C YPT52
View Details	Gavin AC, et al. (2002)		ACT1 ADA2 ADE5,7 ADH1 ARP4 ARP8 CLU1 DPB2 DPB4 ESC8 FUN19 GCN1 GCN5 HEM13 HHF1, HHF2 IES1 IES5 INO80 IOC3 ISW1 ISW2 ITC1 KAP114 KAP123 MED4 MES1 MNN4 MOT1 MYO1 NAP1 NGG1 NHP10 NOP1 NPL6 PDC1 PFK2 POL2 PSK1 PTC1 PUF6 REB1 RET1 RFA1 RFX1 RNR2 RPA135 RPB2 RPC34 RPC40 RPC82 RPN9 RPO21 RPO31 RPT5 RSC2 RSC3 RSC4 RSC58 RSC6 RSC8 RVB2 SFH1 SGF29 SGF73 SIN3 SIN4 SPT15 SPT20 SPT7 SPT8 STH1 STO1 TAF1 TAF10 TAF11 TAF12 TAF13 TAF14 TAF2 TAF3 TAF4 TAF5 TAF6 TAF7 TAF8 TAF9 TBF1 TEF4 TFC7 TOP1 VAS1 VPS1 YBR090C-A, NHP6B YEF3
View Details	Ho Y, et al. (2002)		LTP1 MOT1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	YPT53	MOT1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	MOT1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..266]	MSA	1.011923	View MSA. No confident structure predictions are available.
2	View Details	[267..460]	deduced	N/A	No confident structure predictions are available.
3	View Details	[461..689]	MSA	1.01494	View MSA. No confident structure predictions are available.
4	View Details	[690..1018]	deduced	N/A	No confident structure predictions are available.
5	View Details	[1019..1198]	MSA	2.326994	View MSA. No confident structure predictions are available.
6	View Details	[1199..1269]	deduced	N/A	No confident structure predictions are available.
7	View Details	[1270..1583]	Pfam	151.040959	SNF2 family N-terminal domain No confident structure predictions are available.
8	View Details	[1584..1672]	deduced	N/A	No confident structure predictions are available.
9	View Details	[1673..1746]	Pfam	24.638272	Helicase conserved C-terminal domain No confident structure predictions are available.
10	View Details	[1747..1867]	MSA	7.580996	View MSA. No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
binding	2.96084803796407	bayes_pls_golite062009
nucleic acid binding	2.69616509670156	bayes_pls_golite062009
protein binding	2.66176697577398	bayes_pls_golite062009
transcription regulator activity	2.19067997822967	bayes_pls_golite062009
protein transporter activity	2.04344254357091	bayes_pls_golite062009
DNA binding	1.96172460362671	bayes_pls_golite062009
RNA binding	1.78069099517213	bayes_pls_golite062009
structural molecule activity	1.66038601649515	bayes_pls_golite062009
transcription factor activity	1.21493607378314	bayes_pls_golite062009
transcription activator activity	1.11944470571412	bayes_pls_golite062009
cytoskeletal protein binding	0.66737572008694	bayes_pls_golite062009
translation regulator activity	0.642878560112121	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.620348955429476	bayes_pls_golite062009
protein transmembrane transporter activity	0.395743822582218	bayes_pls_golite062009
structure-specific DNA binding	0.384349606267362	bayes_pls_golite062009
substrate-specific transporter activity	0.36250570104201	bayes_pls_golite062009
translation initiation factor activity	0.286790642914928	bayes_pls_golite062009
nuclear localization sequence binding	0.135350548248851	bayes_pls_golite062009
mRNA binding	0.107084556647027	bayes_pls_golite062009
transcription factor binding	8.79739237675814E-4	bayes_pls_golite062009

Term	Confidence	Notes
DNA-dependent ATPase activity	2.72462789867829	bayes_pls_golite062009
binding	2.64537357529343	bayes_pls_golite062009
nucleic acid binding	2.51683451033161	bayes_pls_golite062009
nucleoside-triphosphatase activity	2.36915495988847	bayes_pls_golite062009
pyrophosphatase activity	2.29436982996465	bayes_pls_golite062009
DNA helicase activity	2.2817164193758	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	2.27303683020046	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	2.27190707957159	bayes_pls_golite062009
DNA binding	2.12258000781149	bayes_pls_golite062009
transcription regulator activity	2.09345198747025	bayes_pls_golite062009
ATP-dependent DNA helicase activity	1.88455339138556	bayes_pls_golite062009
ATPase activity	1.76238574289941	bayes_pls_golite062009
helicase activity	1.70242731830224	bayes_pls_golite062009
microtubule motor activity	1.6537049553461	bayes_pls_golite062009
ATPase activity, coupled	1.57826779898847	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	1.56989255954747	bayes_pls_golite062009
structural constituent of ribosome	1.47858140861683	bayes_pls_golite062009
RNA helicase activity	1.42791449104435	bayes_pls_golite062009
RNA-dependent ATPase activity	1.39335733374328	bayes_pls_golite062009
ATP-dependent RNA helicase activity	1.36724390116598	bayes_pls_golite062009
motor activity	1.34881124154297	bayes_pls_golite062009
protein-DNA loading ATPase activity	1.29640539057136	bayes_pls_golite062009
structure-specific DNA binding	1.2539059700735	bayes_pls_golite062009
protein binding	1.22129154759482	bayes_pls_golite062009
purine NTP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
ATP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
DNA clamp loader activity	1.12431517057397	bayes_pls_golite062009
double-stranded DNA binding	1.117585097627	bayes_pls_golite062009
DNA-directed DNA polymerase activity	1.02269224635866	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
DNA polymerase activity	0.987648886158896	bayes_pls_golite062009
hydrolase activity	0.98565595572967	bayes_pls_golite062009
transcription factor activity	0.887108427603557	bayes_pls_golite062009
structural molecule activity	0.86514677273731	bayes_pls_golite062009
transcription repressor activity	0.862685397469217	bayes_pls_golite062009
purine nucleotide binding	0.725611289205392	bayes_pls_golite062009
nucleotide binding	0.717697610309727	bayes_pls_golite062009
purine ribonucleotide binding	0.713814218071151	bayes_pls_golite062009
ribonucleotide binding	0.713752628021599	bayes_pls_golite062009
ATP binding	0.60527666756293	bayes_pls_golite062009
adenyl ribonucleotide binding	0.573214556107072	bayes_pls_golite062009
single-stranded DNA binding	0.568457189190858	bayes_pls_golite062009
adenyl nucleotide binding	0.554530910579109	bayes_pls_golite062009
lysine N-acetyltransferase activity	0.551232230388384	bayes_pls_golite062009
histone acetyltransferase activity	0.551232230388384	bayes_pls_golite062009
transcription activator activity	0.537911793669378	bayes_pls_golite062009
cytoskeletal protein binding	0.500462538667228	bayes_pls_golite062009
	0.442119153778711	bayes_pls_golite062009
GTPase activity	0.437665353870877	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	0.407727781673696	bayes_pls_golite062009
mismatched DNA binding	0.31144582840038	bayes_pls_golite062009
translation regulator activity	0.29096092258568	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.274994961137851	bayes_pls_golite062009
RNA polymerase II transcription factor activity	0.243902356228423	bayes_pls_golite062009
substrate-specific transporter activity	0.144154288308693	bayes_pls_golite062009
centromeric DNA binding	0.120185906020979	bayes_pls_golite062009
active transmembrane transporter activity	0.114463153989353	bayes_pls_golite062009
microfilament motor activity	0.10236059675512	bayes_pls_golite062009
RNA binding	0.0261282189186349	bayes_pls_golite062009
actin binding	0.00482938540552702	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2