Yeast Taf170 is encoded by MOT1 and exists in a TATA box-binding protein (TBP)-TBP-associated factor complex distinct from transcription factor IID

J Biol Chem. 1994 Sep 16;269(37):23135-40.

Abstract

Our characterization of the Saccharomyces cerevisiae TATA box-binding protein (TBP) has led to the identification of nine specific yeast TBP-associated factors (TAFs) ranging in size from 170 to 25 kDa. The amino acid sequence derived from a purified TAF with an apparent M(r) of 170,000 indicates that yeast Taf170 is encoded by the essential yeast gene MOT1. We describe in this report a series of experiments that demonstrate that the protein encoded by MOT1 is a bona fide yeast TAF and that Taf170 forms a separate complex with TBP distinct from the RNA polymerase II-specific multisubunit transcription factor IID TBP-TAF complex. The significance of this unique TBP-Taf170 complex regarding transcriptional regulation is discussed.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphatases
  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Western
  • Cloning, Molecular
  • DNA Helicases / genetics*
  • DNA Helicases / isolation & purification
  • DNA Helicases / metabolism
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Electrophoresis, Polyacrylamide Gel
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Precipitin Tests
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins*
  • TATA Box
  • TATA-Binding Protein Associated Factors*
  • TATA-Box Binding Protein
  • Transcription Factor TFIID
  • Transcription Factors / genetics*
  • Transcription Factors / isolation & purification
  • Transcription Factors / metabolism

Substances

  • DNA-Binding Proteins
  • Oligodeoxyribonucleotides
  • Saccharomyces cerevisiae Proteins
  • TATA-Binding Protein Associated Factors
  • TATA-Box Binding Protein
  • Transcription Factor TFIID
  • Transcription Factors
  • Adenosine Triphosphatases
  • MOT1 protein, S cerevisiae
  • DNA Helicases