Protein Overview: EHD3

General Information:
Name(s) found:	EHD3 / YDR036C [SGD]
Description(s) found: Found 29 descriptions. SHOW ALL	3-hydroxyisobutyryl-CoA hydrolase, member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis [SGD]
Organism:	Saccharomyces cerevisiae
Length:	500 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA ]
Biological Process:	[NOT]fatty acid beta-oxidation [IMP] endocytosis [IMP]
Molecular Function:	3-hydroxyisobutyryl-CoA hydrolase activity [IDA][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MLRNTLKCAQ LSSKYGFKTT TRTFMTTQPQ LNVTDAPPVL FTVQDTARVI TLNRPKKLNA  60
   61 LNAEMSESMF KTLNEYAKSD TTNLVILKSS NRPRSFCAGG DVATVAIFNF NKEFAKSIKF 120
  121 FTDEYSLNFQ IATYLKPIVT FMDGITMGGG VGLSIHTPFR IATENTKWAM PEMDIGFFPD 180
  181 VGSTFALPRI VTLANSNSQM ALYLCLTGEV VTGADAYMLG LASHYVSSEN LDALQKRLGE 240
  241 ISPPFNNDPQ SAYFFGMVNE SIDEFVSPLP KDYVFKYSNE KLNVIEACFN LSKNGTIEDI 300
  301 MNNLRQYEGS AEGKAFAQEI KTKLLTKSPS SLQIALRLVQ ENSRDHIESA IKRDLYTAAN 360
  361 MCMNQDSLVE FSEATKHKLI DKQRVPYPWT KKEQLFVSQL TSITSPKPSL PMSLLRNTSN 420
  421 VTWTQYPYHS KYQLPTEQEI AAYIEKRTND DTGAKVTERE VLNHFANVIP SRRGKLGIQS 480
  481 LCKIVCERKC EEVNDGLRWK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		EHD3 FYV4 MRP1 MRP13 MRP21 MRP4 MRP51 MRPS16 MRPS17 MRPS18 MRPS28 MRPS9 NAM9 PUS7 RSM10 RSM22 RSM23 RSM24 RSM25 RSM26 RSM27 RSM7
View Details	Gavin AC, et al. (2006)		EHD3 MRP1 MRP13 MRP17 MRP21 MRP4 MRP51 MRPS16 MRPS17 MRPS18 MRPS28 MRPS5 MRPS8 MRPS9 NAM9 PET123 RSM10 RSM19 RSM22 RSM23 RSM25 RSM27 RSM7
View Details	Gavin AC, et al. (2002)		BEM2 CCM1 ECM16 EHD3 FYV4 GEP3 HSC82 MRP1 MRP10 MRP13 MRP17 MRP21 MRP4 MRP51 MRPS16 MRPS17 MRPS18 MRPS28 MRPS5 MRPS8 MRPS9 NAM9 PAP2 PET123 PSD2 PUS7 RIX1 RSM10 RSM19 RSM22 RSM23 RSM24 RSM25 RSM26 RSM27 RSM7 TIF4631 UBP10 UTP20 UTP22
View Details	Ho Y, et al. (2002)		ADH2 AFG2 AIM21 ALB1 ARX1 BUD20 CIC1 COF1 CPR1 EHD3 FYV4 GPI15 HHF1, HHF2 HMO1 HTA1 HTB1 HTB2 HYP2 LHP1 LSM2 MAM33 MDH1 MGM101 MNP1 MRPS16 NMD3 NOG1 NOP12 NOP13 NOP7 NSA2 NUG1 OYE2 PRP43 PUF6 PWP1 RIX1 RLP24 RSM24 RSM25 TEF4 UTP20 YBL044W YFL006W
View Details	Qiu et al. (2008)		EHD3 MRP1 MRP10 MRP13 MRP17 MRP21 MRP4 MRP51 MRPL8 MRPS16 MRPS17 MRPS18 MRPS28 MRPS35 MRPS5 MRPS9 NAM9 PET123 PPE1 RSM10 RSM18 RSM19 RSM22 RSM23 RSM24 RSM25 RSM26 RSM27 RSM7 VAR1 YMR31

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	EHD3	DIG1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	EHD3	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..247]	PSI-BLAST	188.201249	Enoyl-CoA hydratase (crotonase)
2	View Details	[248..345]	PSI-BLAST	188.201249	Enoyl-CoA hydratase (crotonase)
3	View Details	[346..433]	PSI-BLAST	3.30103	Short chain L-3-hydroxyacyl CoA dehydrogenase
4	View Details	[434..500]	deduced	N/A	Confident ab initio structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
long-chain-3-hydroxyacyl-CoA dehydrogenase activity	4.80462645980977	bayes_pls_golite062009
long-chain-enoyl-CoA hydratase activity	4.09442487131529	bayes_pls_golite062009
CoA carboxylase activity	3.89913842968597	bayes_pls_golite062009
acetyl-CoA carboxylase activity	3.85567083340773	bayes_pls_golite062009
acetyl-CoA C-acyltransferase activity	3.6090947563188	bayes_pls_golite062009
ligase activity, forming carbon-carbon bonds	3.4980371783229	bayes_pls_golite062009
oxidoreductase activity	3.09625241707629	bayes_pls_golite062009
catalytic activity	1.79741889688267	bayes_pls_golite062009
oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor	1.70038641098437	bayes_pls_golite062009
oxidoreductase activity, acting on CH-OH group of donors	1.65338499044318	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-CH group of donors	1.61759001796638	bayes_pls_golite062009
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	1.47309297191111	bayes_pls_golite062009
3-hydroxyacyl-CoA dehydrogenase activity	1.25489401408719	bayes_pls_golite062009
propionyl-CoA carboxylase activity	1.20853698666984	bayes_pls_golite062009
oxidoreductase activity, acting on NADH or NADPH	0.99118526697655	bayes_pls_golite062009
transferase activity, transferring acyl groups	0.788034697021177	bayes_pls_golite062009
methylcrotonoyl-CoA carboxylase activity	0.718702926356989	bayes_pls_golite062009
monovalent inorganic cation transmembrane transporter activity	0.576163737662951	bayes_pls_golite062009
enoyl-CoA hydratase activity	0.439912268968251	bayes_pls_golite062009
binding	0.355184540089764	bayes_pls_golite062009
L-malate dehydrogenase activity	0.332985872550541	bayes_pls_golite062009
methylenetetrahydrofolate dehydrogenase activity	0.124826575912808	bayes_pls_golite062009
methenyltetrahydrofolate cyclohydrolase activity	0.118312276550651	bayes_pls_golite062009
fatty acid synthase activity	0.0962368786590111	bayes_pls_golite062009
intramolecular oxidoreductase activity, transposing C=C bonds	0.0918189681606029	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	0.0913823272876781	bayes_pls_golite062009
alcohol dehydrogenase (NAD) activity	0.00644214957417527	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.87

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2