Protein Overview: SPT20

General Information:
Name(s) found:	SPT20 / YOL148C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|722281\|gb\|AAB07900.1\| Spt20p [NCBI NR] gi\|6324424\|ref\|NP_014493.1\| Subunit of the SAGA transcriptional regulatory complex, involved in maintaining the integrity of the complex; Spt20p [Saccharomyces cerevisiae] [NCBI NR] gi\|1711485\|sp\|P50875.1\|SPT20_YEAST RecName: Full=Transcription factor SPT20 [NCBI NR] gi\|1420052\|emb\|CAA99169.1\| SPT20 [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S58643 probable transcription factor SPT20 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1244722\|gb\|AAB07899.1\| Ada5p [NCBI NR] Transcription factor SPT20 OS=Saccharomyces cerevisiae GN=SPT20 PE=1 SV=1 [Swiss-Prot] (P50875) Transcription factor SPT20 [Swiss-Prot] SPT20 Member of the TBP class of SPT proteins that alter transcription site selection [mips] Subunit of the SAGA transcriptional regulatory complex, involved in maintaining the integrity of the complex [SGD] Chr XV from 47572-45758, reverse complement [hmr_yeast5_DM3-1_pombe.fasta] Chr XV from 45758-47572, reverse complement [yeastorf3.fasta] SPT20, Chr XV from 45758-47572, reverse complement [yeast_orfs2] SPT20 SGDID:S0005508, Chr XV from 47572-45758, reverse complement, Verified ORF [yeastflysequest.fasta] Spt20p [Saccharomyces cerevisiae]gi\|1420052\|emb\|CAA99169.1\| SPT20 [Saccharomyces cerevisiae]gi\|1711485\|sp\|P50875\|SP20_YEAST Transcription factor SPT20gi\|1362474\|pir\|\|S58643 probable transcription factor SPT20 - yeast (Saccharomyces cerevisiae)gi\|72228 [nrdb_11152004_Chlamydomonas] SPT20 SGDID:S000005508, Chr XV from 47572-45758, reverse complement, Verified ORF, "Subunit of the SAGA transcriptional regulatory complex, involved in maintaining the integrity of the complex" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] SPT20 SGDID:S000005508, Chr XV from 47573-45759, reverse complement, Verified ORF, "Subunit of the SAGA transcriptional regulatory complex, involved in maintaining the integrity of the complex" [yeast-contam.fasta] Subunit of the SAGA transcriptional regulatory complex, involved in maintaining the integrity of the complex; Spt20p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Spt20p [nr-271106-contam.fasta] SPT20 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Ada5p [nr-271106-contam.fasta] Transcription factor SPT20 [nr-271106-contam.fasta] [chemostatdb.fasta] Transcription factor SPT20 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SPT20 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SPT20 SGDID:S000005508, Chr XV from 47574-45760, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of the SAGA transcriptional regulatory complex; involved in maintaining the integrity of the complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay" [yeast-030211-contam.fasta] SPT20 SGDID:S000005508, Chr XV from 47574-45760, Genome Release 64-2-1, reverse complement, Verified ORF, "Subunit of the SAGA transcriptional regulatory complex; involved in maintaining the integrity of the complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay" [20141208_orf_trans.fasta] YOL148C SGDID:S000005508, Chr XV from 47574-45760, Genome Release 64-1-1, reverse complement, Verified ORF, "Subunit of the SAGA transcriptional regulatory complex; involved in maintaining the integrity of the complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	604 amino acids
Gene Ontology:
Cellular Component:	SLIK (SAGA-like) complex [IPI] SAGA complex [IDA]
Biological Process:	chromatin modification [TAS] histone acetylation [TAS]
Molecular Function:	transcription cofactor activity [IGI][IMP][IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSANSPTGND PHVFGIPVNA TPSNMGSPGS PVNVPPPMNP AVANVNHPVM RTNSNSNANE  60
   61 GTRTLTREQI QQLQQRQRLL LQQRLLEQQR KQQALQNYEA QFYQMLMTLN KRPKRLYNFV 120
  121 EDADSILKKY EQYLHSFEFH IYENNYKICA PANSRLQQQQ KQPELTSDGL ILTKNNETLK 180
  181 EFLEYVARGR IPDAIMEVLR DCNIQFYEGN LILQVYDHTN TVDVTPKENK PNLNSSSSPS 240
  241 NNNSTQDNSK IQQPSEPNSG VANTGANTAN KKASFKRPRV YRTLLKPNDL TTYYDMMSYA 300
  301 DNARFSDSIY QQFESEILTL TKRNLSLSVP LNPYEHRDML EETAFSEPHW DSEKKSFIHE 360
  361 HRAESTREGT KGVVGHIEER DEFPQHSSNY EQLMLIMNER TTTITNSTFA VSLTKNAMEI 420
  421 ASSSSNGVRG ASSSTSNSAS NTRNNSLANG NQVALAAAAA AAAVGSTMGN DNNQFSRLKF 480
  481 IEQWRINKEK RKQQALSANI NPTPFNARIS MTAPLTPQQQ LLQRQQQALE QQQNGGAMKN 540
  541 ANKRSGNNAT SNNNNNNNNL DKPKVKRPRK NAKKSESGTP APKKKRMTKK KQSASSTPSS 600
  601 TTMS

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ADA2 GCN5 HFI1 NGG1 SGF11 SGF29 SGF73 SPT20 SPT3 SPT7 SPT8 TAF10 TAF12 TAF5 TAF6 TAF9 TRA1 UBP8
View Details	(MIPS) Mewes HW, et al. (2004)		ADA2 GCN5 HFI1 NGG1 SGF29 SGF73 SPT20 SPT3 SPT7 SPT8 TAF10 TAF12 TAF5 TAF6 TAF9 TRA1
View Details	Krogan NJ, et al. (2006)		ADA2 AHC1 AHC2 FMP40 GCN5 HFI1 MSS4 NGG1 PDR15 RAD59 REV3 SGF11 SGF29 SGF73 SNL1 SPT20 SPT3 SPT7 SPT8 TAF1 TAF10 TAF11 TAF12 TAF13 TAF3 TAF4 TAF5 TAF6 TAF7 TAF9 UBP8 YMR007W
View Details	Gavin AC, et al. (2006)		ADA2 HFI1 NGG1 SGF29 SGF73 SPT20 SPT3 SPT7 SPT8
View Details	Gavin AC, et al. (2002)		ADA2 ADH1 CLU1 FUN19 GCN5 HEM13 HFI1 KAP114 KAP123 MED4 MES1 MNN4 MOT1 MYO1 MYO4 NGG1 NOP1 PFK2 PSK1 PTC1 PUF6 RPB2 RPN9 RPO21 RPT5 RVB2 SGF29 SGF73 SIN3 SIN4 SPT15 SPT20 SPT7 SPT8 TAF1 TAF10 TAF11 TAF12 TAF13 TAF14 TAF2 TAF3 TAF4 TAF5 TAF6 TAF7 TAF8 TAF9 TBF1 TEF4 UBP8 VAS1 VPS1
View Details	Qiu et al. (2008)		ADA2 ARP4 CSE2 GAL11 GCN5 HFI1 MED2 MED6 NGG1 NUT1 NUT2 PGD1 SGF11 SGF29 SGF73 SIN4 SPT20 SPT3 SPT7 SPT8 SRB4 SRB5 SRB6 SRB7 SUS1 TAF1 TAF10 TAF11 TAF12 TAF13 TAF14 TAF2 TAF3 TAF5 TAF6 TAF8 TAF9 TFB5 TRA1 UBP8

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CAB4	No Comments	Hazbun TR, et al. (2003)
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CAB3	SPT20	1	Hazbun TR, et al. (2003)
View Screen	CAB3	SPT20	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	SPT20	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..604]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.93

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2