Protein Overview: CDC25

General Information:
Name(s) found:	CDC25 / YLR310C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6323341\|ref\|NP_013413.1\| Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression th [NCBI NR] gi\|577222\|gb\|AAB67360.1\| Cdc25p: regulatory protein of adenylate cyclase [Saccharomyces cerevisiae] [NCBI NR] pir\|\|RGBYC5 cell division control protein CDC25 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|171185\|gb\|AAA34478.1\| CDC25 protein [NCBI NR] gi\|115914\|sp\|P04821.1\|CDC25_YEAST RecName: Full=Cell division control protein 25 [NCBI NR] Cell division control protein 25 OS=Saccharomyces cerevisiae GN=CDC25 PE=1 SV=1 [Swiss-Prot] (P04821) Cell division control protein 25 [Swiss-Prot] CDC25 GDP/GTP exchange factor for Ras1p and Ras2p [mips] Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 [SGD] CDC25, Chr XII from 752224-756993, reverse complement [yeast_orfs2] Chr XII from 752224-756993, reverse complement [yeastorf3.fasta] Chr XII from 756993-752224, reverse complement [yeastorf5.fasta] CDC25 SGDID:S0004301, Chr XII from 756995-752226, reverse complement, Verified ORF [yeastflysequest.fasta] Cdc25p [Saccharomyces cerevisiae]gi\|115914\|sp\|P04821\|CC25_YEAST Cell division control protein 25gi\|279596\|pir\|\|RGBYC5 cell division control protein CDC25 - yeast (Saccharomyces cerevisiae)gi\|577222\|gb\|AAB67360.1\| Cdc25p: regulatory protein of adenylate [nrdb_11152004_Chlamydomonas] CDC25 SGDID:S000004301, Chr XII from 756995-752226, reverse complement, Verified ORF, "Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulati [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1; Cdc25p [Saccharomy [nr-271106-contam.fasta] Cdc25p: regulatory protein of adenylate cyclase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] CDC25 protein [nr-271106-contam.fasta] Cell division control protein 25 [nr-271106-contam.fasta] CDC25 SGDID:S000004301, Chr XII from 756995-752226, reverse complement, Verified ORF, "Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Cell division control protein 25 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CDC25 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] CDC25 SGDID:S000004301, Chr XII from 756993-752224, Genome Release 64-1-1, reverse complement, Verified ORF, "Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1" [yeast-030211-contam.fasta] CDC25 SGDID:S000004301, Chr XII from 756993-752224, Genome Release 64-2-1, reverse complement, Verified ORF, "Membrane bound guanine nucleotide exchange factor; indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1; a membrane bound guanine nucleotide exchange factor is also known as a GEF or GDP-release factor" [20141208_orf_trans.fasta] YLR310C SGDID:S000004301, Chr XII from 756993-752224, Genome Release 64-1-1, reverse complement, Verified ORF, "Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1589 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA] plasma membrane [IDA]
Biological Process:	traversing start control point of mitotic cell cycle [IMP] Ras protein signal transduction [TAS] replicative cell aging [IMP]
Molecular Function:	Ras guanyl-nucleotide exchange factor activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSDTNTSIPN TSSAREAGNA SQTPSISSSS NTSTTTNTES SSASLSSSPS TSELTSIRPI  60
   61 GIVVAAYDFN YPIKKDSSSQ LLSVQQGETI YILNKNSSGW WDGLVIDDSN GKVNRGWFPQ 120
  121 NFGRPLRDSH LRKHSHPMKK YSSSKSSRRS SLNSLGNSAY LHVPRNPSKS RRGSSTLSAS 180
  181 LSNAHNAETS SGHNNTVSMN NSPFSAPNDA SHITPQSSNF NSNASLSQDM TKSADGSSEM 240
  241 NTNAIMNNNE TNLQTSGEKA GPPLVAEETI KILPLEEIEM IINGIRSNIA STWSPIPLIT 300
  301 KTSDYKLVYY NKDLDIYCSE LPLISNSIME SDDICDSEPK FPPNDHLVNL YTRDLRKNAN 360
  361 IEDSSTRSKQ SESEQNRSSL LMEKQDSKET DGNNNSINDD DNNNENNKNE FNEAGPSSLN 420
  421 SLSAPDLTQN IQSRVVAPSR SSILAKSDIF YHYSRDIKLW TELQDLTVYY TKTAHKMFLK 480
  481 ENRLNFTKYF DLISDSIVFT QLGCRLMQHE IKAKSCSKEI KKIFKGLISS LSRISINSHL 540
  541 YFDSAFHRKK MDTMNDKDND NQENNCSRTE GDDGKIEVDS VHDLVSVPLS GKRNVSTSTT 600
  601 DTLTPMRSSF STVNENDMEN FSVLGPRNSV NSVVTPRTSI QNSTLEDFSP SNKNFKSAKS 660
  661 IYEMVDVEFS KFLRHVQLLY FVLQSSVFSD DNTLPQLLPR FFKGSFSGGS WTNPFSTFIT 720
  721 DEFGNATKNK AVTSNEVTAS SSKNSSISRI PPKMADAIAS ASGYSANSET NSQIDLKASS 780
  781 AASGSVFTPF NRPSHNRTFS RARVSKRKKK YPLTVDTLNT MKKKSSQIFE KLNNATGEHL 840
  841 KIISKPKSRI RNLEINSSTY EQINQNVLLL EILENLDLSI FINLKNLIKT PSILLDLESE 900
  901 EFLVHAMSSV SSVLTEFFDI KQAFHDIVIR LIMTTQQTTL DDPYLFSSMR SNFPVGHHEP 960
  961 FKNISNTPLV KGPFHKKNEQ LALSLFHVLV SQDVEFNNLE FLNNSDDFKD ACEKYVEISN1020
 1021 LACIIVDQLI EERENLLNYA ARMMKNNLTA ELLKGEQEKW FDIYSEDYSD DDSENDEAII1080
 1081 DDELGSEDYI ERKAANIEKN LPWFLTSDYE TSLVYDSRGK IRGGTKEALI EHLTSHELVD1140
 1141 AAFNVTMLIT FRSILTTREF FYALIYRYNL YPPEGLSYDD YNIWIEKKSN PIKCRVVNIM1200
 1201 RTFLTQYWTR NYYEPGIPLI LNFAKMVVSE KIPGAEDLLQ KINEKLINEN EKEPVDPKQQ1260
 1261 DSVSAVVQTT KRDNKSPIHM SSSSLPSSAS SAFFRLKKLK LLDIDPYTYA TQLTVLEHDL1320
 1321 YLRITMFECL DRAWGTKYCN MGGSPNITKF IANANTLTNF VSHTIVKQAD VKTRSKLTQY1380
 1381 FVTVAQHCKE LNNFSSMTAI VSALYSSPIY RLKKTWDLVS TESKDLLKNL NNLMDSKRNF1440
 1441 VKYRELLRSV TDVACVPFFG VYLSDLTFTF VGNPDFLHNS TNIINFSKRT KIANIVEEII1500
 1501 SFKRFHYKLK RLDDIQTVIE ASLENVPHIE KQYQLSLQVE PRSGNTKGST HASSASGTKT1560
 1561 AKFLSEFTDD KNGNFLKLGK KKPPSRLFR

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CDC25 MDS3 SAP185 YBR225W
View Details	Krogan NJ, et al. (2006)		CDC25 MDS3 YBR225W
View Details	Gavin AC, et al. (2006)		CDC25 HRK1 MDS3 SAP185 SIT4
View Details	Gavin AC, et al. (2002)		ACC1 AVT4 BEM2 CCT2 CDC25 FAB1 HRK1 MDS3 MIA40 MRPL3 SAP155 SAP185 SAP190 SIT4 SWT21 YBR225W
View Details	Ho Y, et al. (2002)		CDC25 CKA2 CKI1 DCP2 EDC3 EDE1 ENP1 FPR3 GCR2 GIN4 GLC7 HOM3 HRR25 IRA1 IRA2 MDS3 MYO2 MYO4 NAP1 PMD1 PRS2 PRS3 PRS5 RIM11 RPM2 SEC23 SFB3 SGM1 SIT4 TGL1 TPS1 TSL1 TSR1 YDR170W-A YER138C YER160C YJR027W YJR028W

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CWC2	No Comments	Hazbun TR, et al. (2003)
View Run	NSE5	No Comments	Hazbun TR, et al. (2003)
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SWC4	CDC25	1	Hazbun TR, et al. (2003)
View Screen	YLL037W	CDC25	1	Hazbun TR, et al. (2003)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	CDC25	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..129]	PSI-BLAST	47.69897	c-src protein tyrosine kinase; c-src tyrosine kinase
2	View Details	[130..256]	PSI-BLAST	47.69897	c-src protein tyrosine kinase; c-src tyrosine kinase
3	View Details	[257..975]	deduced	N/A	No confident structure predictions are available.
4	View Details	[976..1079]	PSI-BLAST	3.221849	Son of sevenless-1 (sos-1)
5	View Details	[1080..1263] [1467..1494]	PSI-BLAST	488.0	Son of sevenless protein homolog 1 (sos-1)
6	View Details	[1264..1466] [1495..1589]	PSI-BLAST	488.0	Son of sevenless protein homolog 1 (sos-1)

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
signal transducer activity	3.13508395312263	bayes_pls_golite062009
molecular transducer activity	3.13508395312263	bayes_pls_golite062009
binding	3.12724619324809	bayes_pls_golite062009
protein binding	2.14586566310919	bayes_pls_golite062009
molecular adaptor activity	1.81145713371725	bayes_pls_golite062009
SH3/SH2 adaptor activity	1.59474164216385	bayes_pls_golite062009
receptor binding	1.02111138016453	bayes_pls_golite062009
protein binding, bridging	0.83823250804321	bayes_pls_golite062009
kinase regulator activity	0.69502098407037	bayes_pls_golite062009
transcription regulator activity	0.540818657754841	bayes_pls_golite062009
enzyme regulator activity	0.456661966897198	bayes_pls_golite062009
nucleic acid binding	0.352421112301971	bayes_pls_golite062009
DNA binding	0.281643350069021	bayes_pls_golite062009
phosphoprotein binding	0.16798254471727	bayes_pls_golite062009
cytoskeletal protein binding	0.0898978787421796	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
binding	3.29080729281876	bayes_pls_golite062009
protein binding	2.12168271674167	bayes_pls_golite062009
GTPase regulator activity	1.66986263418539	bayes_pls_golite062009
nucleoside-triphosphatase regulator activity	1.64990514605397	bayes_pls_golite062009
signal transducer activity	1.55319485371003	bayes_pls_golite062009
molecular transducer activity	1.55319485371003	bayes_pls_golite062009
small GTPase regulator activity	1.2550809703725	bayes_pls_golite062009
enzyme regulator activity	1.25381967078645	bayes_pls_golite062009
protein domain specific binding	0.90578029954417	bayes_pls_golite062009
small GTPase binding	0.8507658747425	bayes_pls_golite062009
GTPase binding	0.82889812790119	bayes_pls_golite062009
guanyl-nucleotide exchange factor activity	0.745321902095093	bayes_pls_golite062009
receptor binding	0.50127063593663	bayes_pls_golite062009
receptor activity	0.353510125274044	bayes_pls_golite062009
transcription regulator activity	0.324566338715835	bayes_pls_golite062009
transmembrane receptor activity	0.207829786337892	bayes_pls_golite062009
transmembrane receptor protein tyrosine kinase signaling protein activity	0.13700176999256	bayes_pls_golite062009
DNA binding	0.136435190086451	bayes_pls_golite062009
nucleic acid binding	0.118897432176337	bayes_pls_golite062009
receptor signaling protein activity	0.10105990547756	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2