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Protein Overview: SSL2

General Information:
Name(s) found: SSL2 / YIL143C [SGD]
Description(s) found:
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  • gi|6322048|ref|NP_012123.1| Component of the holoenzyme form of RNA polymerase transcription factor TFIIH, has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; involved in DNA repair; homolog of human ERCC3; [NCBI NR]
  • gi|557780|emb|CAA86135.1| DNA repair helicase [Saccharomyces cerevisiae] [NCBI NR]
  • gi|51013217|gb|AAT92902.1| YIL143C [Saccharomyces cerevisiae] [NCBI NR]
  • pir||S31272 DNA excision repair cross-complementing protein ERCC3 [validated] - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • gi|267033|sp|Q00578.1|RAD25_YEAST RecName: Full=DNA repair helicase RAD25; AltName: Full=General transcription and DNA repair factor IIH subunit RAD25; Short=TFIIH subunit RAD25 [NCBI NR]
  • gi|172724|gb|AAA35102.1| encodes human ERCC-3 homologue [Saccharomyces cerevisiae] [NCBI NR]
  • DNA repair helicase RAD25 OS=Saccharomyces cerevisiae GN=RAD25 PE=1 SV=1 [Swiss-Prot]
  • (Q00578) DNA repair helicase RAD25 [Swiss-Prot]
  • SSL2 DNA helicase [mips]
  • Component of the holoenzyme form of RNA polymerase transcription factor TFIIH, has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; involved in DNA repair; homolog of human ERCC3 [SGD]
  • SSL2, Chr IX from 80510-83041, reverse complement [yeast_orfs2]
  • Chr IX from 80510-83041, reverse complement [yeastorf3.fasta]
  • Chr IX from 83041-80510, reverse complement [yeastorf5.fasta]
  • SSL2 SGDID:S0001405, Chr IX from 83041-80510, reverse complement, Verified ORF [yeastflysequest.fasta]
  • DNA helicase homolog; homolog of human XPBC, ERCC3 [Saccharomyces cerevisiae]gi|51013217|gb|AAT92902.1| YIL143C [Saccharomyces cerevisiae]gi|557780|emb|CAA86135.1| DNA repair helicase [Saccharomyces cerevisiae]gi|267033|sp|Q00578|RA25_YEAST DNA repair [nrdb_11152004_Chlamydomonas]
  • SSL2 SGDID:S000001405, Chr IX from 83041-80510, reverse complement, Verified ORF, "Component of the holoenzyme form of RNA polymerase transcription factor TFIIH, has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promote [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • DNA repair helicase [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • DNA repair helicase RAD25 (General transcription and DNA repair factor IIH subunit RAD25) (TFIIH subunit RAD25) [nr-271106-contam.fasta]
  • Component of the holoenzyme form of RNA polymerase transcription factor TFIIH, has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; involved in DNA repair; homolog of human ERCC3; Ssl2p [Saccharomyces cerevis [nr-271106-contam.fasta]
  • YIL143C [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • encodes human ERCC-3 homologue [nr-271106-contam.fasta]
  • SSL2 SGDID:S000001405, Chr IX from 83041-80510, reverse complement, Verified ORF, "Component of the holoenzyme form of RNA polymerase transcription factor TFIIH, has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; involved in DNA repair; homolog of human ERCC3" [yeast-bovrabprots.fasta]
  • [chemostatdb.fasta]
  • DNA repair helicase RAD25 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SSL2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • SSL2 SGDID:S000001405, Chr IX from 83041-80510, Genome Release 64-1-1, reverse complement, Verified ORF, "Component of the holoenzyme form of RNA polymerase transcription factor TFIIH, has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; involved in DNA repair; homolog of human ERCC3" [yeast-030211-contam.fasta]
  • SSL2 SGDID:S000001405, Chr IX from 83041-80510, Genome Release 64-2-1, reverse complement, Verified ORF, "Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; relocalizes to the cytosol in response to hypoxia; homolog of human ERCC3" [20141208_orf_trans.fasta]
  • YIL143C SGDID:S000001405, Chr IX from 83041-80510, Genome Release 64-1-1, reverse complement, Verified ORF, "Component of the holoenzyme form of RNA polymerase transcription factor TFIIH, has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; involved in DNA repair; homolog of human ERCC3" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 843 amino acids

    Gene Ontology:
    Cellular Component: holo TFIIH complex [IDALink to PubMed articleLink to PubMed article]
    nucleotide-excision repair factor 3 complex [IDALink to PubMed article]
    Biological Process: negative regulation of transcription from RNA polymerase II promoter, mitotic [TASLink to PubMed article]
    nucleotide-excision repair, DNA duplex unwinding [TASLink to PubMed article]
    nucleotide-excision repair [IDALink to PubMed article]
    transcription from RNA polymerase II promoter [IDALink to PubMed article]
    transcription initiation from RNA polymerase II promoter [TASLink to PubMed article]
    Molecular Function: DNA helicase activity [TASLink to PubMed article]
    general RNA polymerase II transcription factor activity [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTDVEGYQPK SKGKIFPDMG ESFFSSDEDS PATDAEIDEN YDDNRETSEG RGERDTGAMV  60
   61 TGLKKPRKKT KSSRHTAADS SMNQMDAKDK ALLQDTNSDI PADFVPDSVS GMFRSHDFSY 120
  121 LRLRPDHASR PLWISPSDGR IILESFSPLA EQAQDFLVTI AEPISRPSHI HEYKITAYSL 180
  181 YAAVSVGLET DDIISVLDRL SKVPVAESII NFIKGATISY GKVKLVIKHN RYFVETTQAD 240
  241 ILQMLLNDSV IGPLRIDSDH QVQPPEDVLQ QQLQQTAGKP ATNVNPNDVE AVFSAVIGGD 300
  301 NEREEEDDDI DAVHSFEIAN ESVEVVKKRC QEIDYPVLEE YDFRNDHRNP DLDIDLKPST 360
  361 QIRPYQEKSL SKMFGNGRAR SGIIVLPCGA GKTLVGITAA CTIKKSVIVL CTSSVSVMQW 420
  421 RQQFLQWCTL QPENCAVFTS DNKEMFQTES GLVVSTYSMV ANTRNRSHDS QKVMDFLTGR 480
  481 EWGFIILDEV HVVPAAMFRR VVSTIAAHAK LGLTATLVRE DDKIGDLNFL IGPKLYEANW 540
  541 MELSQKGHIA NVQCAEVWCP MTAEFYQEYL RETARKRMLL YIMNPTKFQA CQFLIQYHER 600
  601 RGDKIIVFSD NVYALQEYAL KMGKPFIYGS TPQQERMNIL QNFQYNDQIN TIFLSKVGDT 660
  661 SIDLPEATCL IQISSHYGSR RQEAQRLGRI LRAKRRNDEG FNAFFYSLVS KDTQEMYYST 720
  721 KRQAFLVDQG YAFKVITHLH GMENIPNLAY ASPRERRELL QEVLLKNEEA AGIEVGDDAD 780
  781 NSVGRGSNGH KRFKSKAVRG EGSLSGLAGG EDMAYMEYST NKNKELKEHH PLIRKMYYKN 840
  841 LKK

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details (MIPS) Mewes HW, et al. (2004) CCL1 KIN28 RAD2 RAD3 SSL1 SSL2 TFB1 TFB2 TFB3 TFB4
    View Details Krogan NJ, et al. (2006) FOL3 PKC1 SSL2 TYR1
    View Details Gavin AC, et al. (2006) GLN4 SSL2 YOR352W
    View Details Ho Y, et al. (2002) MKT1 SSL2 STI1
    View Details Qiu et al. (2008) ABF1 CCL1 ELC1 KIN28 RAD2 RAD3 ROX3 SSL1 SSL2 TAF10 TAF12 TAF2 TAF7 TFA2 TFB1 TFB2 TFB3 TFB4 TFB5 TOA2

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data SSL2 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..297] deduced N/A No confident structure predictions are available.
    2 View Details [298..570] PSI-BLAST 94.39794 Putative DEAD box RNA helicase
    3 View Details [571..717] PSI-BLAST 94.39794 Putative DEAD box RNA helicase
    4 View Details [718..843] PSI-BLAST 10.30103 Nucleotide excision repair enzyme UvrB

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2 No functions predicted.
    3
    Term Confidence Notes
  • general RNA polymerase II transcription factor activity
    		•  5.67074370996647 bayes_pls_golite062009
  • transcription regulator activity
    		•  5.55329976718261 bayes_pls_golite062009
  • DNA binding
    		•  5.08959163553491 bayes_pls_golite062009
  • nucleic acid binding
    		•  4.99681957904233 bayes_pls_golite062009
  • transcription factor activity
    		•  4.33152142980289 bayes_pls_golite062009
  • transcription activator activity
    		•  3.70589483230587 bayes_pls_golite062009
  • sequence-specific DNA binding
    		•  3.58179858678904 bayes_pls_golite062009
  • binding
    		•  3.52874320222492 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  2.7405324052133 bayes_pls_golite062009
  • RNA polymerase II transcription factor activity
    		•  2.60738445921725 bayes_pls_golite062009
  • transcription repressor activity
    		•  2.22485867684613 bayes_pls_golite062009
  • RNA polymerase activity
    		•  1.82365519759474 bayes_pls_golite062009
  • DNA-directed RNA polymerase activity
    		•  1.82365519759474 bayes_pls_golite062009
  • transcription elongation regulator activity
    		•  1.81270943531115 bayes_pls_golite062009
  • protein binding
    		•  1.80415286835431 bayes_pls_golite062009
  • structure-specific DNA binding
    		•  1.38449475025903 bayes_pls_golite062009
  • RNA binding
    		•  0.992043085614693 bayes_pls_golite062009
  • transcription factor binding
    		•  0.85714789329232 bayes_pls_golite062009
  • specific RNA polymerase II transcription factor activity
    		•  0.66743181561551 bayes_pls_golite062009
  • transferase activity
    		•  0.382878181629158 bayes_pls_golite062009
  • double-stranded DNA binding
    		•  0.349632742873003 bayes_pls_golite062009
  • nucleotidyltransferase activity
    		•  0.237727660028553 bayes_pls_golite062009
  • RNA polymerase II transcription factor activity, enhancer binding
    		•  0.23177057841536 bayes_pls_golite062009
  • hydrolase activity
    		•  0.140191206949582 bayes_pls_golite062009
  • positive transcription elongation factor activity
    		•  0.13524243942225 bayes_pls_golite062009
    4 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle