Protein Overview: RRP43

General Information:
Name(s) found:	RRP43 / YCR035C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|32363489\|sp\|P25359.2\|RRP43_YEAST RecName: Full=Exosome complex component RRP43; AltName: Full=Ribosomal RNA-processing protein 43 [NCBI NR] gi\|14588945\|emb\|CAC42984.1\| rRNA processing protein [Saccharomyces cerevisiae] [NCBI NR] gi\|10383796\|ref\|NP_009964.2\| Protein involved in rRNA processing; component of the exosome 3->5 exonuclease complex with Rrp41p, Rrp42p, Rrp4p and Dis3p; required for efficient maturation of 5.8S, 18S and 25S rRNA; Rrp43p [Saccharomyces cerevisiae] [NCBI NR] Exosome complex component RRP43 OS=Saccharomyces cerevisiae GN=RRP43 PE=1 SV=2 [Swiss-Prot] (P25359) Exosome complex exonuclease RRP43 (EC 3.1.13.-) (Ribosomal RNA processing protein 43) [Swiss-Prot] RRP43 rRNA processing protein [mips] Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8) [SGD] RRP43, Chr III from 191826-193010, reverse complement [yeast_orfs2] Chr III from 191826-193010, reverse complement [yeastorf3.fasta] Chr III from 193010-191826, reverse complement [yeastorf5.fasta] RRP43 SGDID:S0000631, Chr III from 193013-191829, reverse complement, Verified ORF [yeastflysequest.fasta] Ribosomal RNA Processing; Component of the exosome 3->5 exoribonuclease complex with Rrp4p, Rrp41p, Rrp42p and Dis3p (Rrp44p). [Saccharomyces cerevisiae]gi\|14588945\|emb\|CAC42984.1\| rRNA processing protein [Saccharomyces cerevisiae]gi\|32363489\|sp\|P25359\| [nrdb_11152004_Chlamydomonas] RRP43 SGDID:S000000631, Chr III from 193013-191829, reverse complement, Verified ORF, "Protein involved in rRNA processing; component of the exosome 3->5 exonuclease complex with Rrp41p, Rrp42p, Rrp4p and Dis3p; required for efficient maturation of 5.8S, [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] RRP43 SGDID:S000000631, Chr III from 193014-191830, reverse complement, Verified ORF, "Protein involved in rRNA processing; component of the exosome 3->5 exonuclease complex with Rrp41p, Rrp42p, Rrp4p and Dis3p; required for efficient maturation of 5.8S, [yeast-contam.fasta] RRP43 SGDID:S000000631, Chr III from 193014-191830, reverse complement, Verified ORF, "Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to [yeast-200209-contam.fasta] Exosome complex exonuclease RRP43 (Ribosomal RNA-processing protein 43) [nr-271106-contam.fasta] Protein involved in rRNA processing; component of the exosome 3->5 exonuclease complex with Rrp41p, Rrp42p, Rrp4p and Dis3p; required for efficient maturation of 5.8S, 18S and 25S rRNA; Rrp43p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] rRNA processing protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] RRP43 SGDID:S000000631, Chr III from 193014-191830, reverse complement, Verified ORF, "Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8)" [yeast-bovrabprots.fasta] RRP43 SGDID:S000000631, Chr III from 193014-191830, reverse complement, Verified ORF, "Protein involved in rRNA processing; component of the exosome 3->5 exonuclease complex with Rrp41p, Rrp42p, Rrp4p and Dis3p; required for efficient maturation of 5.8S, 18S and 25S rRNA" [yeast-contam_custom.fasta] [chemostatdb.fasta] Exosome complex component RRP43 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RRP43 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] RRP43 SGDID:S000000631, Chr III from 193018-191834, Genome Release 64-1-1, reverse complement, Verified ORF, "Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8)" [yeast-030211-contam.fasta] RRP43 SGDID:S000000631, Chr III from 193018-191834, Genome Release 64-2-1, reverse complement, Verified ORF, "Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress" [20141208_orf_trans.fasta] YCR035C SGDID:S000000631, Chr III from 193018-191834, Genome Release 64-1-1, reverse complement, Verified ORF, "Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8)" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	394 amino acids
Gene Ontology:
Cellular Component:	cytoplasmic exosome (RNase complex) [IDA] nuclear exosome (RNase complex) [IDA] nuclear outer membrane [TAS]
Biological Process:	mRNA catabolic process [IPI]
Molecular Function:	3'-5'-exoribonuclease activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAESTTLETI EIHPITFPPE VLARISPELS LQRHLSLGIR PCLRKYEEFR DVAIENNTLS  60
   61 RYADAGNIDT KNNILGSNVL KSGKTIVITS ITGGIIEETS AAIKDLDDFG EEELFEVTKE 120
  121 EDIIANYASV YPVVEVERGR VGACTDEEMT ISQKLHDSIL HSRILPKKAL KVKAGVRSAN 180
  181 EDGTFSVLYP DELEDDTLNE TNLKMKRKWS YVLYAKIVVL SRTGPVFDLC WNSLMYALQS 240
  241 VKLPRAFIDE RASDLRMTIR TRGRSATIRE TYEIICDQTK SVPLMINAKN IAFASNYGIV 300
  301 ELDPECQLQN SDNSEEEEVD IDMDKLNTVL IADLDTEAEE TSIHSTISIL AAPSGNYKQL 360
  361 TLVGGGAKIT PEMIKRSLLL SRVRADDLST RFNI

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CSL4 DIS3 LRP1 MPP6 MTR3 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 SKI2 SKI3 SKI6 SKI7 SRP1
View Details	(MIPS) Mewes HW, et al. (2004)		DIS3 RRP4 RRP42 RRP43 RRP45 RRP46 SKI6
View Details	Krogan NJ, et al. (2006)		CSL4 DDI1 DIS3 ENT3 LRP1 MPP6 MTR3 OSM1 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 SKI6 SKI7 YBL055C
View Details	Gavin AC, et al. (2006)		CSL4 DIS3 MTR3 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 SKI6 SKI7
View Details	Gavin AC, et al. (2002)		CMD1 CSL4 CTR9 DIS3 ECM16 GCN1 LRP1 MAM33 MLC1 MTR3 MYO2 NMD5 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 SAM1 SHE4 SKI2 SKI3 SKI6 SKI7 SRP1 UTP10 UTP20 UTP22 YIR035C
View Details	Ho Y, et al. (2002)		DIS3 EAF3 FIP1 HAS1 HPR1 KAP95 MES1 MFT1 NAM8 NOP6 NUP1 NUP2 NUP60 PAP1 PCT1 REB1 RNT1 RRP4 RRP43 RRP6 RTT103 SIF2 SIN3 SNU56 SRP1 STO1 THO2 TRA1 UME1 YPR090W
View Details	Qiu et al. (2008)		CSL4 DIS3 MTR3 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 SKI6

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	RRP43 and NOP56	Huh WK, et al. (2003)
View Data	RRP43	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..277]	Pfam	42.026872	3' exoribonuclease family, domain 1 No confident structure predictions are available.
2	View Details	[278..394]	Pfam	14.455932	3' exoribonuclease family, domain 2 No confident structure predictions are available.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Protein Overview: RRP43

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: