Protein Overview: BUD3

General Information:
Name(s) found:	BUD3 / YCL014W [SGD]
Description(s) found: Found 23 descriptions. SHOW ALL	Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding [SGD]
Organism:	Saccharomyces cerevisiae
Length:	1636 amino acids
Gene Ontology:
Cellular Component:	cellular bud neck contractile ring [TAS] cellular bud neck [IDA]
Biological Process:	axial cellular bud site selection [TAS] cellular bud site selection [TAS] cytokinesis [IMP][TAS]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MEKDLSSLYS EKKDKENDET LFNIKLSKSV VETTPLNGHS LFDDDKSLSD WTDNVFTQSV  60
   61 FYHGSDDLIW GKFFVCVYKS PNSNKLNAII FDKLGTSCFE SVDISSNSQY YPAIENLSPS 120
  121 DQESNVKKCI AVILLQRYPL LSPSDLSQIL SNKSENCDYD PPYAGDLASS CQLITAVPPE 180
  181 DLGKRFFTSG LLQNRFVSST LLDVIYENNE STIELNNRLV FHLGEQLEQL FNPVTEYSPE 240
  241 QTEYGYKAPE DELPTESDDD LVKAICNELL QLQTNFTFNL VEFLQKFLIA LRVRVLNEEI 300
  301 NGLSTTKLNR LFPPTIDEVT RINCIFLDSL KTAIPYGSLE VLKACSITIP YFYKAYTRHE 360
  361 AATKNFSKDI KLFIRHFSNV IPEREVYTEM KIESIIKGPQ EKLLKLKLII ERLWKSKKWR 420
  421 PKNQEMAKKC YNNIIDVIDS FGKLDSPLHS YSTRVFTPSG KILTELAKCW PVELQYKWLK 480
  481 RRVVGVYDVV DLNDENKRNL LVIFSDYVVF INILEAESYY TSDGSNRPLI SDILMNSLIN 540
  541 EVPLPSKIPK LKVERHCYID EVLVSILDKS TLRFDRLKGK DSFSMVCKLS SAFISSSSVA 600
  601 DLITKARILE KDTAFHLFKA SRSHFTLYST AHELCAYDSE KIKSKFALFL NIPPSKEILE 660
  661 VNNLHLAFFA RFCSNDGRDN IVILDVLTKH DDKHIEVTSD NIVFTIINQL AIEIPICFSS 720
  721 LNSSMAKDLL CVNENLIKNL EHQLEEVKHP STDEHRAVNS KLSGASDFDA THEKKRSYGT 780
  781 ITTFRSYTSD LKDSPSGDNS NVTKETKEIL PVKPTKKSSK KPREIQKKTK TNASKAEHIE 840
  841 KKKPNKGKGF FGVLKNVFGS KSKSKPSPVQ RVPKKISQRH PKSPVKKPMT SEKKSSPKRA 900
  901 VVSSPKIKKK STSFSTKESQ TAKSSLRAVE FKSDDLIGKP PDVGNGAHPQ ENTRISSVVR 960
  961 DTKYVSYNPS QPVTENTSNE KNVEPKADQS TKQDNISNFA DVEVSASSYP EKLDAETDDQ1020
 1021 IIGKATNSSS VHGNKELPDL AEVTTANRVS TTSAGDQRID TQSEFLRAAD VENLSDDDEH1080
 1081 RQNESRVFND DLFGDFIPKH YRNKQENINS SSNLFPEGKV PQEKGVSNEN TNISLKTNED1140
 1141 ASTLTQKLSP QASKVLTENS NELKDTNNEG KDAKDIKLGD DYSDKETAKE ITKPKNFVEG1200
 1201 ITERKEIFPT IPRLAPPASK INFQRSPSYI ELFQGMRVVL DKHDAHYNWK RLASQVSLSE1260
 1261 GLKVNTEEDA AIINKSQDDA KAERMTQISE VIEYEMQQPI PTYLPKAHLD DSGIEKSDDK1320
 1321 FFEIEEELKE ELKGSKTGNE DVGNNNPSNS IPKIEKPPAF KVIRTSPVRI IGRTFEDTRK1380
 1381 YENGSPSDIS FTYDTHNNDE PDKRLMELKF PSQDEIPDDR FYTPAEEPTA EFPVEELPNT1440
 1441 PRSINVTTSN NKSTDDKLSS GNIDQKPTEL LDDLEFSSFN IAFGNTSMST DNMKISSDLS1500
 1501 SNKTVLGNAQ KVQESPSGPL IYVLPQSSTK HEKEGFLRKK QKDEPIWVSP SKIDFADLSR1560
 1561 RTKALTPERN TVPLKNNDSR KYKYTGEGSI GNMTNMLLTK DASYAYLKDF VALSDDEDED1620
 1621 GKQNCAVGGP EKLKFY

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		BUD3 HXK1 MRPL20 TDH1
View Details	Krogan NJ, et al. (2006)		AHA1 BUD3 CNS1 CPR6 DIG2 DNM1 DSF1, YNR073C FRT2 GPA2 HOM6 HSC82 HSP104 HSP82 MBB1 MRPL50 PAM1 PPT1 QNS1 RMD8 RTS1 SMT3 STI1 STP4 TDH1 UTP20 YBL104C
View Details	Gavin AC, et al. (2006)		ATG26 BUD3 MRPL10 MRPL16 MRPL23 MRPL4 MRPL51 SEC1
View Details	Gavin AC, et al. (2002)		ATG26 BEM2 BUD3 COP1 CYR1 HRR25 IMG1 IMG2 IML1 MHR1 MRH4 MRM1 MRP20 MRP49 MRP7 MRPL1 MRPL10 MRPL13 MRPL16 MRPL17 MRPL19 MRPL20 MRPL23 MRPL24 MRPL25 MRPL27 MRPL28 MRPL3 MRPL35 MRPL36 MRPL39 MRPL4 MRPL44 MRPL51 MRPL6 MRPL7 MRPL8 NUG1 PRP43 RET2 ROM2 RTC6 SEC1 SEC26 SEC27 SIN4 SNF2 SWI3 SYP1 TAF5 UTP20 YDR115W YML6
View Details	Ho Y, et al. (2002)		BRX1 BUD3 CCT2 CCT3 CCT6 CIN8 CLU1 HIR1 HTB1 HTB2 IPI3 KRE33 MNP1 NAN1 NOP56 POL5 RIX1 RPC19 RRP12 RVB1 SSD1 TIF4631 TMA19 UTP10 UTP22 UTP7
View Details	Qiu et al. (2008)		BNR1 BUD3 BUD4 HOF1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	STE3	BUD3	1	Unpublished Fields Lab Data
View Screen	SPT21	BUD3	1	Unpublished Fields Lab Data
View Screen	PGA1	BUD3	1	Unpublished Fields Lab Data
View Screen	FMP41	BUD3	1	Unpublished Fields Lab Data
View Screen	PPG1	BUD3	1	Unpublished Fields Lab Data
View Screen	ILM1	BUD3	1	Lockshon D, et al. (2006)
View Screen	ARF1	BUD3	1	Unpublished Fields Lab Data
View Screen	YHR022C	BUD3	1	Unpublished Fields Lab Data
View Screen	CDC23	BUD3	1	Unpublished Fields Lab Data
View Screen	CAB2	BUD3	1	Unpublished Fields Lab Data
View Screen	TRL1	BUD3	1	Unpublished Fields Lab Data
View Screen	RCY1	BUD3	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	BUD3	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..240]	deduced	N/A	No confident structure predictions are available.
2	View Details	[241..635]	MSA	1.006863	View MSA. No confident structure predictions are available.
3	View Details	[636..1558]	MSA	27.337997	View MSA. No confident structure predictions are available.
4	View Details	[1559..1636]	deduced	N/A	No confident structure predictions are available.
5	View Details	[1038..1216]	MSA	1.422993	View MSA. No confident structure predictions are available.
6	View Details	[1217..1278]	deduced	N/A	No confident structure predictions are available.
7	View Details	[1279..1534]	MSA	1.393995	View MSA. No confident structure predictions are available.
8	View Details	[1535..1636]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2