Protein Overview: RPL4A

General Information:
Name(s) found:	RPL4A / YBR031W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6319505\|ref\|NP_009587.1\| N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Bp and has similarity to E. coli L4 and rat L4 ribosomal proteins; Rpl4ap [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S45887 ribosomal protein L4.e.A, cytosolic - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|585880\|sp\|P10664.4\|RL4A_YEAST RecName: Full=60S ribosomal protein L4-A; AltName: Full=L2; AltName: Full=YL2; AltName: Full=RP2 [NCBI NR] gi\|536244\|emb\|CAA84973.1\| RPL2A [Saccharomyces cerevisiae] [NCBI NR] gi\|498759\|emb\|CAA53687.1\| ribosomal protein L2B [Saccharomyces cerevisiae] [NCBI NR] gi\|1587588\|prf\|\|2206497L ribosomal protein L2B [NCBI NR] 60S ribosomal protein L4-A OS=Saccharomyces cerevisiae GN=RPL4A PE=1 SV=4 [Swiss-Prot] RPL4A Ribosomal protein [mips] N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Bp and has similarity to E. coli L4 and rat L4 ribosomal proteins [SGD] RPL4A, Chr II from 300125-301213 [yeast_orfs2] Chr II from 300125-301213 [yeastorf3.fasta] RPL4A SGDID:S0000235, Chr II from 300128-301216, Verified ORF [yeastflysequest.fasta] RPL4A SGDID:S0000235, Chr II from 300166-301254, Verified ORF [10112005cervSGD.fasta] N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Bp and has similarity to E. coli L4 and rat L4 ribosomal proteins [Saccharomyces cerevisiae]gi\|536244\|emb\|CAA84973.1\| RPL2A [Saccharomyces cerevisiae] [nrdb_11152004_Chlamydomonas] RPL4A SGDID:S000000235, Chr II from 300166-301254, Verified ORF, "N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Bp and has similarity to E. coli L4 and rat L4 ribosomal proteins" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] ribosomal protein L2B [Saccharomyces cerevisiae] [nr-271106-contam.fasta] N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Bp and has similarity to E. coli L4 and rat L4 ribosomal proteins; Rpl4ap [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ribosomal protein L2B [nr-271106-contam.fasta] 60S ribosomal protein L4-A (L2A) (RP2) [nr-271106-contam.fasta] RPL2A [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] 60S ribosomal protein L4-A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPL4A PE=1 SV=4 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] RPL4A SGDID:S000000235, Chr II from 300166-301254, Genome Release 64-1-1, Verified ORF, "N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Bp and has similarity to E. coli L4 and rat L4 ribosomal proteins" [yeast-030211-contam.fasta] RPL4A SGDID:S000000235, Chr II from 300166-301254, Genome Release 64-2-1, Verified ORF, "Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YBR031W SGDID:S000000235, Chr II from 300166-301254, Genome Release 64-1-1, Verified ORF, "N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Bp and has similarity to E. coli L4 and rat L4 ribosomal proteins" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_795\|g.395 ORF asmbl_795\|g.395 asmbl_795\|m.395 type:complete len:363 (+) asmbl_795:465-1553(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_393\|g.526 ORF ID=asmbl_393\|g.526 ID=asmbl_393\|m.526 type:complete len:363 (+) ID=asmbl_393:110-1198(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_799\|g.240 ORF ID=asmbl_799\|g.240 ID=asmbl_799\|m.240 type:complete len:363 (+) ID=asmbl_799:465-1553(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	362 amino acids
Gene Ontology:
Cellular Component:	ribonucleoprotein complex [IEA] cytoplasm [IEA] cytosolic large ribosomal subunit [TAS] ribosome [IEA] intracellular [IEA]
Biological Process:	translation [TAS]
Molecular Function:	structural constituent of ribosome [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSRPQVTVHS LTGEATANAL PLPAVFSAPI RPDIVHTVFT SVNKNKRQAY AVSEKAGHQT  60
   61 SAESWGTGRA VARIPRVGGG GTGRSGQGAF GNMCRGGRMF APTKTWRKWN VKVNHNEKRY 120
  121 ATASAIAATA VASLVLARGH RVEKIPEIPL VVSTDLESIQ KTKEAVAALK AVGAHSDLLK 180
  181 VLKSKKLRAG KGKYRNRRWT QRRGPLVVYA EDNGIVKALR NVPGVETANV ASLNLLQLAP 240
  241 GAHLGRFVIW TEAAFTKLDQ VWGSETVASS KVGYTLPSHI ISTSDVTRII NSSEIQSAIR 300
  301 PAGQATQKRT HVLKKNPLKN KQVLLRLNPY AKVFAAEKLG SKKAEKTGTK PAAVFTETLK 360
  361 HD

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ABF2 BFR1 GAR1 HCA4 ISA2 NHP6A NOP53 NOP56 PBP4 PMA1 PUF4 RAD4 RPF2 RPL10 RPL11B RPL12B, RPL12A RPL15A RPL16A RPL16B RPL17A RPL17B RPL18A, RPL18B RPL1A, RPL1B RPL20A RPL21A RPL24A RPL26A RPL29 RPL2A, RPL2B RPL3 RPL30 RPL31B RPL32 RPL34A RPL36A RPL42B, RPL42A RPL43B, RPL43A RPL4A RPL5 RPL6A RPL8B RPL9A RPS11A, RPS11B RPS12 RPS14A RPS16B, RPS16A RPS17B RPS1A RPS2 RPS22A RPS23B, RPS23A RPS26A RPS26B RPS3 RPS30B, RPS30A RPS5 RPS8B, RPS8A RPS9B SBP1 YHI9
View Details	(MIPS) Mewes HW, et al. (2004)		RPL10 RPL11A RPL11B RPL12B, RPL12A RPL13A RPL13B RPL14A RPL14B RPL15A RPL15B RPL16A RPL16B RPL17A RPL17B RPL18A, RPL18B RPL19B, RPL19A RPL1A, RPL1B RPL20A RPL20B RPL21A RPL21B RPL22A RPL22B RPL23B, RPL23A RPL24A RPL24B RPL25 RPL26A RPL26B RPL27A RPL27B RPL28 RPL29 RPL2A, RPL2B RPL3 RPL30 RPL31A RPL31B RPL32 RPL33A RPL33B RPL34A RPL34B RPL35A, RPL35B RPL36A RPL36B RPL37A RPL37B RPL38 RPL39 RPL40B, RPL40A RPL41A, RPL41B RPL42B, RPL42A RPL43B, RPL43A RPL4A RPL4B RPL5 RPL6A RPL6B RPL7A RPL7B RPL8A RPL8B RPL9A RPL9B RPP0 RPP1A RPP1B RPP2A RPP2B
View Details	Gavin AC, et al. (2006)		KTR3 RPL4A
View Details	Qiu et al. (2008)		BUD20 NSA2 RPL10 RPL11A RPL11B RPL12B, RPL12A RPL13A RPL13B RPL14A RPL14B RPL15A RPL15B RPL16A RPL16B RPL17A RPL17B RPL18A, RPL18B RPL19B, RPL19A RPL1A, RPL1B RPL20A RPL21A RPL21B RPL22A RPL22B RPL23B, RPL23A RPL24A RPL24B RPL25 RPL26A RPL26B RPL27A RPL27B RPL28 RPL29 RPL2A, RPL2B RPL3 RPL30 RPL31A RPL31B RPL32 RPL33A RPL33B RPL34A RPL34B RPL35A, RPL35B RPL36A RPL36B RPL37A RPL37B RPL38 RPL39 RPL40B, RPL40A RPL41A, RPL41B RPL42B, RPL42A RPL43B, RPL43A RPL4A RPL4B RPL5 RPL6A RPL6B RPL7A RPL7B RPL8A RPL8B RPL9A RPL9B RPP0 RPP1A RPP1B RPP2A RPP2B RPS0B RPS10A RPS10B RPS11A, RPS11B RPS12 RPS13 RPS14A RPS14B RPS15 RPS16B, RPS16A RPS17A RPS18B, RPS18A RPS1A RPS1B RPS2 RPS21A RPS21B RPS22A RPS22B RPS24A, RPS24B RPS25A RPS25B RPS26A RPS26B RPS27A RPS27B RPS28A RPS28B RPS29A RPS29B RPS3 RPS30B, RPS30A RPS31 RPS4B, RPS4A RPS6A, RPS6B RPS7A RPS7B RPS8B, RPS8A RPS9A RPS9B STM1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MIF2	No Comments	Cheeseman IM, et al. (2002)
View Run	TOR1	No Comments	Reinke A, et al. (2004)
View Run	BIR1	No Comments	Widlund PO, et al. (2005)
View Run	HSL7	No Comments	McCusker D, et al (2007)
View Run	BIR1	No Comments	Widlund PO, et al. (2005)
View Run	KIP3	No Comments	Cheeseman IM, et al. (2002)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	LST8	No Comments	Reinke A, et al. (2004)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	KOG1	No Comments	Reinke A, et al. (2004)
View Run	BIR1	No Comments	Widlund PO, et al. (2005)
View Run	BIR1	No Comments	Widlund PO, et al. (2005)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	HIR2	No Comments	Green EM, et al (2005)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	MIH1	control 1	McCusker D, et al (2007)
View Run	MIH1	control 2	McCusker D, et al (2007)
View Run	MIH1	experimental1 - mih1-1	McCusker D, et al (2007)
View Run	MIH1	experimental2 - mih1-2	McCusker D, et al (2007)
View Run	ASF1	3912: TAP-tagged, strain background includes hpc2(delta)	Green EM, et al (2005)
View Run	RLF2	cac1: TAP-tagged	Green EM, et al (2005)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	RLF2	phosphorylation data - cascade search	Green EM, et al (2005)
View Run	RLF2	identification data - first search in cascade	Green EM, et al (2005)
View Run	ASF1	sample 3928 - Asf1-S-TEV-ZZ (in hir3delta mutant)	Green EM, et al (2005)
View Run	VAM3	sample: hx1	Hao Xu, et al. (2010)
View Run	VAM3	sample: hx2	Hao Xu, et al. (2010)
View Run	VAM3	sample: hx3	Hao Xu, et al. (2010)
View Run	VAM3	sample: hx4	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control).	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX13 - trans-SNARE complex forms but fusion is blocked.	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX14 - mixture in detergent (control).	Hao Xu, et al. (2010)
View Run	VAM3	hx23: mixture in detergent (control) Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex	Hao Xu, et al. (2010)
View Run	MLP2	#19 Asynchronous Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#34 Asynchronous SPB prep	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#04 Alpha Factor Prep2	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#19b Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#19a Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	RPL4A	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..49] [106..300]	PSI-BLAST	1231.045757	No description for 1k5yD_ was found.
2	View Details	[50..105]	PSI-BLAST	1231.045757	No description for 1k5yD_ was found.
3	View Details	[301..362]	MSA	2.025997	View MSA. No confident structure predictions are available.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.56

Protein Overview: RPL4A

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: