Protein Overview: PIN4

General Information:
Name(s) found:	PIN4 / YBL051C [SGD]
Description(s) found: Found 27 descriptions. SHOW ALL	Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage [SGD]
Organism:	Saccharomyces cerevisiae
Length:	668 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA]
Biological Process:	DNA damage checkpoint [IGI][IPI] G2/M transition of mitotic cell cycle [IMP]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 METSSFENAP PAAINDAQDN NINTETNDQE TNQQSIETRD AIDKENGVQT ETGENSAKNA  60
   61 EQNVSSTNLN NAPTNGALDD DVIPNAIVIK NIPFAIKKEQ LLDIIEEMDL PLPYAFNYHF 120
  121 DNGIFRGLAF ANFTTPEETT QVITSLNGKE ISGRKLKVEY KKMLPQAERE RIEREKREKR 180
  181 GQLEEQHRSS SNLSLDSLSK MSGSGNNNTS NNQLFSTLMN GINANSMMNS PMNNTINNNS 240
  241 SNNNNSGNII LNQPSLSAQH TSSSLYQTNV NNQAQMSTER FYAPLPSTST LPLPPQQLDF 300
  301 NDPDTLEIYS QLLLFKDREK YYYELAYPMG ISASHKRIIN VLCSYLGLVE VYDPRFIIIR 360
  361 RKILDHANLQ SHLQQQGQMT SAHPLQPNST GGSMNRSQSY TSLLQAHAAA AANSISNQAV 420
  421 NNSSNSNTIN SNNGNGNNVI INNNSASSTP KISSQGQFSM QPTLTSPKMN IHHSSQYNSA 480
  481 DQPQQPQPQT QQNVQSAAQQ QQSFLRQQAT LTPSSRIPSG YSANHYQINS VNPLLRNSQI 540
  541 SPPNSQIPIN SQTLSQAQPP AQSQTQQRVP VAYQNASLSS QQLYNLNGPS SANSQSQLLP 600
  601 QHTNGSVHSN FSYQSYHDES MLSAHNLNSA DLIYKSLSHS GLDDGLEQGL NRSLSGLDLQ 660
  661 NQNKKNLW

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Gavin AC, et al. (2002)		PIN4 RVB2 TIF1, TIF2
View Details	Ho Y, et al. (2002)		ABP1 ACO1 AIM41 ATP4 BUD14 CAR2 COR1 CRZ1 CYS4 DCP1 DCP2 DNM1 EDC3 EDE1 EGD2 ENP1 GAS1 GCN3 GPH1 HHF1, HHF2 HRR25 HSP104 HXT7 HYP2 IPP1 LOC1 LSP1 LTV1 MDH1 MDS3 MGE1 NSA1 NUG1 OYE2 PEX19 PIL1 PIN4 PTC4 PUF3 RIO2 RPC19 RPM2 SAP185 SAP190 SAS10 SEC2 SEC23 SES1 SFB3 SGM1 SIT4 TMA19 TSR1 VMA4 YBR225W YER138C

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	PIN4	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..74]	PSI-BLAST	47.221849	Poly(A)-binding protein
2	View Details	[75..164]	PSI-BLAST	57.0	Nuclear ribonucleoprotein A1 (RNP A1, UP1)
3	View Details	[165..300]	PSI-BLAST	57.0	Nuclear ribonucleoprotein A1 (RNP A1, UP1)
4	View Details	[301..443]	MSA	2.206995	View MSA. No confident structure predictions are available.
5	View Details	[444..530]	PSI-BLAST	8.522879	poly(A) binding protein
6	View Details	[531..668]	MSA	2.154996	View MSA. No confident structure predictions are available.

Functions predicted (by domain):

#

Gene Ontology predictions

1

Term	Confidence	Notes
nucleic acid binding	3.64594243387558	bayes_pls_golite062009
binding	3.00411894202318	bayes_pls_golite062009
mRNA binding	2.76744192669109	bayes_pls_golite062009
RNA binding	2.41328743949172	bayes_pls_golite062009
protein binding	1.12215421354121	bayes_pls_golite062009
transcription regulator activity	0.737458867828906	bayes_pls_golite062009
DNA binding	0.691258294213766	bayes_pls_golite062009
mRNA 3'-UTR binding	0.599389001184667	bayes_pls_golite062009
translation regulator activity	0.38120065819101	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.128616879457381	bayes_pls_golite062009
structural molecule activity	0.0829058000493267	bayes_pls_golite062009
structure-specific DNA binding	0.0666589824195664	bayes_pls_golite062009

2

Term	Confidence	Notes
nucleic acid binding	3.64594243387558	bayes_pls_golite062009
binding	3.00411894202318	bayes_pls_golite062009
mRNA binding	2.76744192669109	bayes_pls_golite062009
RNA binding	2.41328743949172	bayes_pls_golite062009
protein binding	1.12215421354121	bayes_pls_golite062009
transcription regulator activity	0.737458867828906	bayes_pls_golite062009
DNA binding	0.691258294213766	bayes_pls_golite062009
mRNA 3'-UTR binding	0.599389001184667	bayes_pls_golite062009
translation regulator activity	0.38120065819101	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.128616879457381	bayes_pls_golite062009
structural molecule activity	0.0829058000493267	bayes_pls_golite062009
structure-specific DNA binding	0.0666589824195664	bayes_pls_golite062009

3

Term	Confidence	Notes
binding	1.45278097493666	bayes_pls_golite062009
nucleic acid binding	1.29585787691257	bayes_pls_golite062009
DNA binding	1.21862098093327	bayes_pls_golite062009
transcription regulator activity	1.06027197080527	bayes_pls_golite062009
protein binding	0.741098311996669	bayes_pls_golite062009
catalytic activity	0.307971467423079	bayes_pls_golite062009
structure-specific DNA binding	0.0146459886075777	bayes_pls_golite062009

4

No functions predicted.

5

Term	Confidence	Notes
mRNA binding	2.78281658065802	bayes_pls_golite062009
nucleic acid binding	2.51377464669599	bayes_pls_golite062009
poly(A) RNA binding	2.40208706397043	bayes_pls_golite062009
poly-purine tract binding	2.34978214792168	bayes_pls_golite062009
binding	2.12178506555766	bayes_pls_golite062009
RNA binding	1.38020034067888	bayes_pls_golite062009
single-stranded RNA binding	1.00503950757483	bayes_pls_golite062009
protein binding	0.856075580719879	bayes_pls_golite062009
ligase activity	0.38737123012383	bayes_pls_golite062009
catalytic activity	0.287695754348243	bayes_pls_golite062009
small conjugating protein ligase activity	0.109419310631115	bayes_pls_golite062009
ubiquitin-protein ligase activity	0.0911314408939872	bayes_pls_golite062009
eukaryotic initiation factor 4G binding	0.054315640496152	bayes_pls_golite062009

6

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle