Protein Overview: WTM1

General Information:
Name(s) found:	WTM1 / YOR230W [SGD]
Description(s) found: Found 31 descriptions. SHOW ALL	Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats [SGD]
Organism:	Saccharomyces cerevisiae
Length:	437 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA ]
Biological Process:	regulation of meiosis [IGI]
Molecular Function:	transcription corepressor activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPKKVWKSST PSTYEHISSL RPKFVSRVDN VLHQRKSLTF SNVVVPDKKN NTLTSSVIYS  60
   61 QGSDIYEIDF AVPLQEAASE PVKDYGDAFE GIENTSLSPK FVYQGETVSK MAYLDKTGET 120
  121 TLLSMSKNGS LAWFKEGIKV PIHIVQELMG PATSYASIHS LTRPGDLPEK DFSLAISDFG 180
  181 ISNDTETIVK SQSNGDEEDS ILKIIDNAGK PGEILRTVHV PGTTVTHTVR FFDNHIFASC 240
  241 SDDNILRFWD TRTSDKPIWV LGEPKNGKLT SFDCSQVSNN LFVTGFSTGI IKLWDARAAE 300
  301 AATTDLTYRQ NGEDPIQNEI ANFYHAGGDS VVDVQFSATS SSEFFTVGGT GNIYHWNTDY 360
  361 SLSKYNPDDT IAPPQDATEE SQTKSLRFLH KGGSRRSPKQ IGRRNTAAWH PVIENLVGTV 420
  421 DDDSLVSIYK PYTEESE

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		RNR2 WTM1
View Details	Krogan NJ, et al. (2006)		GIN4 HFD1 HTZ1 KAP114 KCC4 NAP1 PRO3 REI1 RIM11 RPS6A, RPS6B UBC8 VIP1 WTM1 YKR011C
View Details	Gavin AC, et al. (2006)		ARO3 WTM1
View Details	Ho Y, et al. (2002)		AAT2 ACH1 ACO1 ACS2 AFR1 AHP1 ARC1 ARF1 ATP3 CAR2 CCT2 CCT3 CCT6 CDC33 CLU1 COF1 COR1 CPR1 CYR1 CYS4 DLD3 DUG1 EGD1 ERG10 ERG13 ERG6 FPR1 FRS2 FUM1 GND1 GND2 GRX1 GUS1 HEF3 HHF1, HHF2 ILV3 LRO1 LSP1 MET6 NTF2 OLA1 OYE2 PFK1 PIL1 PRM2 RHO1 RNR2 RSN1 RVB1 SAH1 SCP160 SEC53 SEN15 SES1 SNU13 SOD1 TCP1 TEF4 THS1 TIF1, TIF2 TMA19 TSA2 UBA1 VMA4 VMA5 WTM1 WTM2 YNK1 YOR283W

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#06 Alpha Factor Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	IRE1	WTM1	1	Unpublished Fields Lab Data
View Screen	CTF13	WTM1	1	Unpublished Fields Lab Data
View Screen	CUS1	WTM1	1	Unpublished Fields Lab Data
View Screen	PSE1	WTM1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	WTM1	Huh WK, et al. (2003)
View Data	WTM1 and NOP56	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..74]	deduced	N/A	Confident ab initio structure predictions are available.
2	View Details	[75..437]	PSI-BLAST	68.69897	beta1-subunit of the signal-transducing G protein heterotrimer

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
binding	2.42570478946173	bayes_pls_golite062009
transcription regulator activity	2.3610959773142	bayes_pls_golite062009
nucleic acid binding	2.30918780727915	bayes_pls_golite062009
protein binding	2.26925404328312	bayes_pls_golite062009
DNA binding	1.71685536470455	bayes_pls_golite062009
transcription activator activity	1.23969853550045	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	1.21305848427711	bayes_pls_golite062009
cytoskeletal protein binding	1.19593750149343	bayes_pls_golite062009
transcription factor activity	1.08768505897883	bayes_pls_golite062009
	0.88172763425403	bayes_pls_golite062009
transporter activity	0.692270256454453	bayes_pls_golite062009
histone binding	0.685319917508843	bayes_pls_golite062009
RNA polymerase II transcription factor activity	0.676924760416604	bayes_pls_golite062009
transcription repressor activity	0.658573452658359	bayes_pls_golite062009
DNA-dependent ATPase activity	0.612501046529002	bayes_pls_golite062009
actin binding	0.529861581509437	bayes_pls_golite062009
transcription factor binding	0.489473003915035	bayes_pls_golite062009
histone methyltransferase activity	0.311523647180993	bayes_pls_golite062009
transcription cofactor activity	0.30761651528651	bayes_pls_golite062009
structural molecule activity	0.254012051644049	bayes_pls_golite062009
protein kinase activity	0.229242350006544	bayes_pls_golite062009
transcription corepressor activity	0.182612389752591	bayes_pls_golite062009
chromatin binding	0.151977098745772	bayes_pls_golite062009
substrate-specific transporter activity	0.121183207943714	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.00585398682921312	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2