Protein Overview: IRE1

General Information:
Name(s) found:	IRE1 / YHR079C [SGD]
Description(s) found: Found 26 descriptions. SHOW ALL	Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress [SGD]
Organism:	Saccharomyces cerevisiae
Length:	1115 amino acids
Gene Ontology:
Cellular Component:	nuclear envelope [TAS] endoplasmic reticulum membrane [TAS]
Biological Process:	activation of signaling protein activity involved in unfolded protein response [TAS] inositol metabolic process [TAS] UFP-specific transcription factor mRNA processing during unfolded protein response [TAS] protein amino acid phosphorylation [IDA]
Molecular Function:	protein serine/threonine kinase activity [IDA] endoribonuclease activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MRLLRRNMLV LTLLVCVFSS IISCSIPLSS RTSRRQIVED EVASTKKLNF NYGVDKNINS  60
   61 PIPAPRTTEG LPNMKLSSYP TPNLLNTADN RRANKKGRRA ANSISVPYLE NRSLNELSLS 120
  121 DILIAADVEG GLHAVDRRNG HIIWSIEPEN FQPLIEIQEP SRLETYETLI IEPFGDGNIY 180
  181 YFNAHQGLQK LPLSIRQLVS TSPLHLKTNI VVNDSGKIVE DEKVYTGSMR TIMYTINMLN 240
  241 GEIISAFGPG SKNGYFGSQS VDCSPEEKIK LQECENMIVI GKTIFELGIH SYDGASYNVT 300
  301 YSTWQQNVLD VPLALQNTFS KDGMCIAPFR DKSLLASDLD FRIARWVSPT FPGIIVGLFD 360
  361 VFNDLRTNEN ILVPHPFNPG DHESISSNKV YLDQTSNLSW FALSSQNFPS LVESAPISRY 420
  421 ASSDRWRVSS IFEDETLFKN AIMGVHQIYN NEYDHLYENY EKTNSLDTTH KYPPLMIDSS 480
  481 VDTTDLHQNN EMNSLKEYMS PEDLEAYRKK IHEQISRELD EKNQNSLLLK FGSLVYRIIE 540
  541 TGVFLLLFLI FCAILQRFKI LPPLYVLLSK IGFMPEKEIP IVESKSLNCP SSSENVTKPF 600
  601 DMKSGKQVVF EGAVNDGSLK SEKDNDDADE DDEKSLDLTT EKKKRKRGSR GGKKGRKSRI 660
  661 ANIPNFEQSL KNLVVSEKIL GYGSSGTVVF QGSFQGRPVA VKRMLIDFCD IALMEIKLLT 720
  721 ESDDHPNVIR YYCSETTDRF LYIALELCNL NLQDLVESKN VSDENLKLQK EYNPISLLRQ 780
  781 IASGVAHLHS LKIIHRDLKP QNILVSTSSR FTADQQTGAE NLRILISDFG LCKKLDSGQS 840
  841 SFRTNLNNPS GTSGWRAPEL LEESNNLQCQ VETEHSSSRH TVVSSDSFYD PFTKRRLTRS 900
  901 IDIFSMGCVF YYILSKGKHP FGDKYSRESN IIRGIFSLDE MKCLHDRSLI AEATDLISQM 960
  961 IDHDPLKRPT AMKVLRHPLF WPKSKKLEFL LKVSDRLEIE NRDPPSALLM KFDAGSDFVI1020
 1021 PSGDWTVKFD KTFMDNLERY RKYHSSKLMD LLRALRNKYH HFMDLPEDIA ELMGPVPDGF1080
 1081 YDYFTKRFPN LLIGVYMIVK ENLSDDQILR EFLYS

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		IRE1 LYS21

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	TID3	No Comments	Cheeseman IM, et al. (2002)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	IRE1	YDR532C	2	Uetz P, et al. (2000)

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	IRE1	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..471]	ORFEUS	14.89	Methanol dehydrogenase, heavy chain
2	View Details	[472..637]	deduced	N/A	No confident structure predictions are available.
3	View Details	[638..714] [732..747]	PSI-BLAST	253.38764	Cyclin-dependent PK, CDK2
4	View Details	[715..731] [748..1060]	PSI-BLAST	253.38764	Cyclin-dependent PK, CDK2
5	View Details	[1061..1115]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
phosphotransferase activity, alcohol group as acceptor	5.49988294224815	bayes_pls_golite062009
protein kinase activity	5.4586557943719	bayes_pls_golite062009
kinase activity	5.3287080791198	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	4.96052051118534	bayes_pls_golite062009
cyclin-dependent protein kinase activity	4.62392503670521	bayes_pls_golite062009
protein serine/threonine kinase activity	4.46381887831481	bayes_pls_golite062009
transferase activity	3.97138602499442	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	3.06466701648326	bayes_pls_golite062009
binding	2.74863538127007	bayes_pls_golite062009
phosphoinositide 3-kinase binding	2.41610417530517	bayes_pls_golite062009
protein binding	2.276111749106	bayes_pls_golite062009
ATP binding	1.97600611746091	bayes_pls_golite062009
adenyl ribonucleotide binding	1.93596671856664	bayes_pls_golite062009
cation transmembrane transporter activity	1.93176092222748	bayes_pls_golite062009
adenyl nucleotide binding	1.90928021591891	bayes_pls_golite062009
ion transmembrane transporter activity	1.90354666768303	bayes_pls_golite062009
molecular transducer activity	1.79921740914853	bayes_pls_golite062009
signal transducer activity	1.79921740914853	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	1.71452519680794	bayes_pls_golite062009
purine ribonucleotide binding	1.65732943334212	bayes_pls_golite062009
ribonucleotide binding	1.65726906484847	bayes_pls_golite062009
purine nucleotide binding	1.63763394711025	bayes_pls_golite062009
nucleotide binding	1.63195828048121	bayes_pls_golite062009
insulin receptor substrate binding	1.62657794525303	bayes_pls_golite062009
transcription regulator activity	1.56748951434625	bayes_pls_golite062009
nucleic acid binding	1.46547714006072	bayes_pls_golite062009
MAP kinase kinase activity	1.42275431028856	bayes_pls_golite062009
protein tyrosine kinase activity	1.3898059094397	bayes_pls_golite062009
receptor signaling protein serine/threonine kinase activity	1.38496328649031	bayes_pls_golite062009
DNA binding	1.35333135345112	bayes_pls_golite062009
receptor signaling protein activity	1.27291233344322	bayes_pls_golite062009
metal ion transmembrane transporter activity	1.27184436658773	bayes_pls_golite062009
insulin receptor activity	1.22442673174261	bayes_pls_golite062009
catalytic activity	1.21655087018101	bayes_pls_golite062009
transcription factor activity	1.10928254946881	bayes_pls_golite062009
fibroblast growth factor receptor activity	1.01340572255694	bayes_pls_golite062009
transcription activator activity	0.997272622653026	bayes_pls_golite062009
PTB domain binding	0.9900774724568	bayes_pls_golite062009
insulin-like growth factor receptor binding	0.872782211382796	bayes_pls_golite062009
MAP kinase activity	0.81943579965027	bayes_pls_golite062009
insulin binding	0.614254468447749	bayes_pls_golite062009
transporter activity	0.595111702412257	bayes_pls_golite062009
cyclin-dependent protein kinase activating kinase activity	0.529121666062889	bayes_pls_golite062009
protein serine/threonine/tyrosine kinase activity	0.48345772948903	bayes_pls_golite062009
fibroblast growth factor binding	0.470448662251808	bayes_pls_golite062009
protein kinase C activity	0.41587987561486	bayes_pls_golite062009
transmembrane transporter activity	0.41384976209545	bayes_pls_golite062009
RNA polymerase II transcription factor activity	0.401250804041384	bayes_pls_golite062009
magnesium ion binding	0.393328812780268	bayes_pls_golite062009
telethonin binding	0.386946764759301	bayes_pls_golite062009
MAP kinase kinase kinase activity	0.37115026509016	bayes_pls_golite062009
protein complex binding	0.343240719858581	bayes_pls_golite062009
insulin receptor binding	0.266450215230107	bayes_pls_golite062009
cation channel activity	0.245400615103891	bayes_pls_golite062009
SH2 domain binding	0.226760162354251	bayes_pls_golite062009
substrate-specific transporter activity	0.0922946791401962	bayes_pls_golite062009
transmembrane receptor protein kinase activity	0.0556163711947139	bayes_pls_golite062009
transmembrane receptor activity	0.00652065412910241	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.0040394359628233	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2