Protein Overview: NDE1

General Information:
Name(s) found:	NDE1 / YMR145C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|732163\|sp\|P40215.1\|NDH1_YEAST RecName: Full=External NADH-ubiquinone oxidoreductase 1, mitochondrial; AltName: Full=External NADH dehydrogenase 1; Flags: Precursor [NCBI NR] gi\|6323794\|ref\|NP_013865.1\| Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain; Nde1p [Saccharom [NCBI NR] gi\|606443\|emb\|CAA87359.1\| similar to rotenone-insensitive NADH-ubiquinone [Saccharomyces cerevisiae] [NCBI NR] gi\|51013021\|gb\|AAT92804.1\| YMR145C [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S50401 hypothetical protein YMR145c - yeast (Saccharomyces cerevisiae) [NCBI NR] (P40215) Hypothetical 62.8 kDa protein in RPS16A-TIF34 intergenic region [Swiss-Prot] External NADH-ubiquinone oxidoreductase 1, mitochondrial OS=Saccharomyces cerevisiae GN=NDE1 PE=1 SV=1 [Swiss-Prot] NDE1 Mitochondrial cytosolically directed NADH dehydrogenase [mips] Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain [SGD] YMR145C, Chr XIII from 554792-556474, reverse complement [yeast_orfs2] Chr XIII from 554792-556474, reverse complement [yeastorf3.fasta] Chr XIII from 556474-554792, reverse complement [yeastorf5.fasta] NDE1 SGDID:S0004753, Chr XIII from 556474-554792, reverse complement, Verified ORF [yeastflysequest.fasta] Nde1p [Saccharomyces cerevisiae]gi\|51013021\|gb\|AAT92804.1\| YMR145C [Saccharomyces cerevisiae]gi\|606443\|emb\|CAA87359.1\| similar to rotenone-insensitive NADH-ubiquinone [Saccharomyces cerevisiae]gi\|732163\|sp\|P40215\|YM23_YEAST Hypothetical 62.8 kDa protei [nrdb_11152004_Chlamydomonas] NDE1 SGDID:S000004753, Chr XIII from 556474-554792, reverse complement, Verified ORF, "Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] NDE1 SGDID:S000004753, Chr XIII from 556474-554792, reverse complement, Verified ORF, "Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NA [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] similar to rotenone-insensitive NADH-ubiquinone [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain; Nde1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YMR145C [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Hypothetical 62.8 kDa protein in RPS16A-TIF34 intergenic region [nr-271106-contam.fasta] NDE1 SGDID:S000004753, Chr XIII from 556474-554792, reverse complement, Verified ORF, "Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain" [yeast-bovrabprots.fasta] NDE1 SGDID:S000004753, Chr XIII from 556474-554792, reverse complement, Verified ORF, "Mitochondrial external NADH dehydrogenase, catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain" [yeast-contam_custom.fasta] [chemostatdb.fasta] External NADH-ubiquinone oxidoreductase 1, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NDE1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] NDE1 SGDID:S000004753, Chr XIII from 556475-554793, Genome Release 64-1-1, reverse complement, Verified ORF, "Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain" [yeast-030211-contam.fasta] NDE1 SGDID:S000004753, Chr XIII from 556475-554793, Genome Release 64-2-1, reverse complement, Verified ORF, "Mitochondrial external NADH dehydrogenase; type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain; NDE1 has a paralog, NDE2, that arose from the whole genome duplication" [20141208_orf_trans.fasta] TR4643\|c0_g1_i1\|g.3303 ORF TR4643\|c0_g1_i1\|g.3303 TR4643\|c0_g1_i1\|m.3303 type:complete len:561 (+) TR4643\|c0_g1_i1:234-1916(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR7618\|c0_g1_i1\|g.5609 ORF TR7618\|c0_g1_i1\|g.5609 TR7618\|c0_g1_i1\|m.5609 type:complete len:561 (+) TR7618\|c0_g1_i1:219-1901(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YMR145C SGDID:S000004753, Chr XIII from 556475-554793, Genome Release 64-1-1, reverse complement, Verified ORF, "Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_3960\|g.2755 ORF asmbl_3960\|g.2755 asmbl_3960\|m.2755 type:complete len:561 (+) asmbl_3960:232-1914(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_3929\|g.2530 ORF ID=asmbl_3929\|g.2530 ID=asmbl_3929\|m.2530 type:complete len:561 (+) ID=asmbl_3929:232-1914(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_3964\|g.2469 ORF ID=asmbl_3964\|g.2469 ID=asmbl_3964\|m.2469 type:complete len:561 (+) ID=asmbl_3964:232-1914(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	560 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA ][IMP ]
Biological Process:	NADH oxidation [IDA] glucose catabolic process to ethanol [IMP ] chronological cell aging [IMP]
Molecular Function:	NADH dehydrogenase activity [IDA ][ISS ]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MIRQSLMKTV WANSSRFSLQ SKSGLVKYAK NRSFHAARNL LEDKKVILQK VAPTTGVVAK  60
   61 QSFFKRTGKF TLKALLYSAL AGTAYVSYSL YREANPSTQV PQSDTFPNGS KRKTLVILGS 120
  121 GWGSVSLLKN LDTTLYNVVV VSPRNYFLFT PLLPSTPVGT IELKSIVEPV RTIARRSHGE 180
  181 VHYYEAEAYD VDPENKTIKV KSSAKNNDYD LDLKYDYLVV GVGAQPNTFG TPGVYEYSSF 240
  241 LKEISDAQEI RLKIMSSIEK AASLSPKDPE RARLLSFVVV GGGPTGVEFA AELRDYVDQD 300
  301 LRKWMPELSK EIKVTLVEAL PNILNMFDKY LVDYAQDLFK EEKIDLRLKT MVKKVDATTI 360
  361 TAKTGDGDIE NIPYGVLVWA TGNAPREVSK NLMTKLEEQD SRRGLLIDNK LQLLGAKGSI 420
  421 FAIGDCTFHP GLFPTAQVAH QEGEYLAQYF KKAYKIDQLN WKMTHAKDDS EVARLKNQIV 480
  481 KTQSQIEDFK YNHKGALAYI GSDKAIADLA VGEAKYRLAG SFTFLFWKSA YLAMCLSFRN 540
  541 RVLVAMDWAK VYFLGRDSSI

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		BGL2 FAA1 NDE1
View Details	(MIPS) Mewes HW, et al. (2004)		AIM45 ALD4 CIR1 CIR2 CIT1 CYB2 DLD1 FUM1 GUT2 MDH1 NDE1 NDE2 NDI1 SDH1
View Details	Ho Y, et al. (2002)		ACC1 ADH4 ADR1 ARC1 ARP2 AYR1 BGL2 CBP6 CCT6 ENP1 FAA1 FAA4 FRS2 GAR1 GCD11 GCN3 GLT1 GSF2 GSP1 HEM15 HRP1 HTA2 HXT7 KAP104 KTR3 MAE1 MCX1 MRT4 NAB2 NDE1 NPA3 OAC1 PAA1 PET10 PET9 PSD1 RMT2 RNR2 RPT4 SAP155 SAP185 SAP190 SIT4 SLC1 TAP42 TFG2 TRP3 TRP5 VMA8 VPS8 YDL063C YHM2 YMR196W YNL035C
View Details	Qiu et al. (2008)		ALD4 CIR1 FUM1 GUT2 MDH1 NDE1 NDE2 NDI1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	NOP9	No Comments	Hazbun TR, et al. (2003)
View Run	MIA40	No Comments	Hazbun TR, et al. (2003)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SXM1	NDE1	1	Unpublished Fields Lab Data
View Screen	RAD51	NDE1	1	Unpublished Fields Lab Data
View Screen	UBC7	NDE1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	NDE1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..102]	PSI-BLAST	4.0	Flavocytochrome c sulfide dehydrogenase, FCSD, flavin-binding subunit
2	View Details	[103..144] [182..225] [384..458]	PSI-BLAST	119.68867	Dihydrolipoamide dehydrogenase
3	View Details	[145..181] [226..383] [459..466]	PSI-BLAST	119.68867	Dihydrolipoamide dehydrogenase
4	View Details	[467..560]	PSI-BLAST	119.68867	Dihydrolipoamide dehydrogenase

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.52

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2