Protein Overview: RPM2

General Information:
Name(s) found:	RPM2 / YML091C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6323548\|ref\|NP_013619.1\| Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucl [NCBI NR] gi\|575712\|emb\|CAA86647.1\| RPM2 [Saccharomyces cerevisiae] [NCBI NR] gi\|417700\|sp\|Q02773.1\|RPM2_YEAST RecName: Full=Ribonuclease P protein component, mitochondrial; Short=RNase P; Flags: Precursor [NCBI NR] gi\|172442\|gb\|AAA03168.1\| ribonuclease P [NCBI NR] pir\|\|A48773 ribonuclease P (EC 3.1.26.5) precursor, mitochondrial - yeast (Saccharomyces cerevisiae) [NCBI NR] Ribonuclease P protein component, mitochondrial OS=Saccharomyces cerevisiae GN=RPM2 PE=1 SV=1 [Swiss-Prot] RPM2 Ribonuclease P precursor, mitochondrial [mips] Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus [SGD] RPM2, Chr XIII from 87123-90731, reverse complement [yeast_orfs2] Chr XIII from 87123-90731, reverse complement [yeastorf3.fasta] Chr XIII from 90731-87123, reverse complement [yeastorf5.fasta] (Q02773) Ribonuclease P protein component, mitochondrial precursor (EC 3.1.26.5) (RNase P) [4932.FASTACCERV.txt] RPM2 SGDID:S0004556, Chr XIII from 90731-87123, reverse complement, Verified ORF [yeastflysequest.fasta] Protein component of mitochondrial RNase P, along with the mitochondrially-encoded RNA subunit RPM1; Rnase P removes 5' extensions from tRNA precursors; Rpm2p is also involved in maturation of RPM1 and in translation of mitochondrial mRNAs [Saccharomyces [nrdb_11152004_Chlamydomonas] RPM2 SGDID:S000004556, Chr XIII from 90731-87123, reverse complement, Verified ORF, "Protein component of mitochondrial RNase P, along with the mitochondrially-encoded RNA subunit RPM1; Rnase P removes 5' extensions from tRNA precursors; Rpm2p is also inv [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] RPM2 SGDID:S000004556, Chr XIII from 90731-87123, reverse complement, Verified ORF, "Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] RPM2 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Protein component of mitochondrial RNase P, along with the mitochondrially-encoded RNA subunit RPM1; Rnase P removes 5' extensions from tRNA precursors; Rpm2p is also involved in maturation of RPM1 and in translation of mitochondrial mRNAs; Rpm2p [Sacchar [nr-271106-contam.fasta] Ribonuclease P protein component, mitochondrial precursor (RNase P) [nr-271106-contam.fasta] ribonuclease P [nr-271106-contam.fasta] RPM2 SGDID:S000004556, Chr XIII from 90731-87123, reverse complement, Verified ORF, "Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus" [yeast-bovrabprots.fasta] RPM2 SGDID:S000004556, Chr XIII from 90731-87123, reverse complement, Verified ORF, "Protein component of mitochondrial RNase P, along with the mitochondrially-encoded RNA subunit RPM1; Rnase P removes 5' extensions from tRNA precursors; Rpm2p is also involved in maturation of RPM1 and in translation of mitochondrial mRNAs" [yeast-contam_custom.fasta] [chemostatdb.fasta] Ribonuclease P protein component, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPM2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] RPM2 SGDID:S000004556, Chr XIII from 90731-87123, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus" [yeast-030211-contam.fasta] RPM2 SGDID:S000004556, Chr XIII from 90731-87123, Genome Release 64-2-1, reverse complement, Verified ORF, "Protein subunit of mitochondrial RNase P; has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus" [20141208_orf_trans.fasta] YML091C SGDID:S000004556, Chr XIII from 90731-87123, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1202 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] mitochondrial ribonuclease P complex [IDA] mitochondrion [IDA ] cytoplasmic mRNA processing body [IDA]
Biological Process:	RNA processing [IMP] mRNA processing [IGI][IPI] positive regulation of transcription from RNA polymerase II promoter [IDA][IMP] tRNA 5'-leader removal [IDA] mitochondrion organization [IGI] translation [IMP]
Molecular Function:	ribonuclease P activity [IDA] transcription activator activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAFKSFIYSK GYHRSAAQKK TATSFFDSSY QYLRQNQGLV NSDPVLHASH LHPHPVVVAN  60
   61 VNYNNVDDIL HPHDLDSSIN NTNNPLTHEE LLYNQNVSLR SLKQQQSTNY VNNNNNNQHR 120
  121 YYSTGPTLPT NQYDPLNFSN RNFQDLSLKT SQPSVQQPQN EYSLLKDENA PVWKEDTEPC 180
  181 LNKSTYLQTH IDEINRCYEQ KNYNKINSLY QSLKRNDIVP PLEIFTKVLD SLCKRPLDNN 240
  241 DLDNKMYELL TCYQDMINNR LKPPDEIYNI VLLSLFKGSI LAYQFENPNG SDFYKIAIEL 300
  301 FNTTTNDPKQ KSVVKFRNFS KDVLDYNLLA MNIYPGHITL SKAQQVIKSS PAFIKDSFYF 360
  361 IACFSYAKLT NDKFAIKELY EDFRLSLSSG SPDQGLFDDQ FEIYSVILSS FIETGEVELA 420
  421 TNLLDDLVSK IQSSNGLASN ISLLLSSFLI SMSKVDPSKA YEIWFKFHNL NWIPEFSYEF 480
  481 YLVFMANSFQ DWNLTKKIYD YIFPMERNLS PLKKQKLSDY LLHPIGVDSI TTSLLDYSLQ 540
  541 LKDNEVIMKI LEESIVKNFS FDIGIYPFVF NYLREIQCGE DYLMRFIESH AEFIKKSNSI 600
  601 NKFQFLNMIV DNFQSQSLLN KISHAKFFKN FVEDFNLENC ELVSYNGLIS CINNFIKIPK 660
  661 TIKDFPYILE IHAILVTKLF DFDTYPILQN GNNEVLLKFR DQIEHQFKML AQNFCRLNLD 720
  721 PNLLAGVVSQ AMKMVNLDDT ANGQDLLNFF NHPGDWDKSY PLSLGSFIRN SPRGGIREFT 780
  781 KLSKEGYCFD YDTYKELIIK RAINKQIIDK CLEVCPDSIE LKNIVNLMIS KIPGRNLTQL 840
  841 IINNPKFSKV FVPNLRNDSM LKLIENCESL SNFIRICDFP EKFKSIAIQA ENKNAIELIY 900
  901 ERLFDGGKYA DILRYNNIVP VLNLELLLKS CIRSGEFKKY ESLSKKFNDK ISESSKIDIQ 960
  961 LEYLINKNDL KGAFTLFEKT PRELRTPHKT MDLYTFALFL DSFNRNITYY ESPENTLQFA1020
 1021 NILSSQTSFI NLLSTYNLIA HSDHLMNFNV GGMAAKVKKE ILNQMLNNLY DSIRLLSPSI1080
 1081 ENDKSMKEKL REKVKNYCRF KAYLKSPELD MDELKTLVSV ESFLNPFTPS MLFNNLIETI1140
 1141 YINEHASSLV LQNGLIYSLQ QKGLNKILSY LEESFITSGN DANIEKVREF RSLLRKSKPL1200
 1201 QA

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		GDS1 GEP5 IRC19 PET130 RPM2 RRG1 RRG8 YHR054C
View Details	Gavin AC, et al. (2006)		GCN2 LTV1 RPM2
View Details	Gavin AC, et al. (2002)		DCP1 DCP2 EDC3 RPM2
View Details	Ho Y, et al. (2002)		ABP1 ACO1 AIM41 ATP4 BUD14 CAR2 CDC25 CKA2 COR1 CRZ1 CYS4 DCP1 DCP2 DNM1 EDC3 EDE1 EGD2 ENP1 FPR3 GAS1 GCN3 GLC7 GPH1 HHF1, HHF2 HRR25 HSP104 HXT7 HYP2 IPP1 LOC1 LSP1 LTV1 MDH1 MDS3 MGE1 NSA1 NUG1 OYE2 PEX19 PIL1 PIN4 PTC4 PUF3 RIO2 RPC19 RPM2 SAP185 SAP190 SAS10 SEC2 SEC23 SES1 SFB3 SGM1 SIT4 TGL1 TMA19 TSR1 VMA4 YBR225W YER138C
View Details	Qiu et al. (2008)		COR1 NDI1 RPM2

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#02 Alpha Factor Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	YLR415C	RPM2	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	RPM2	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..1202]	deduced	N/A	No confident structure predictions are available.
2	View Details	[170..239]	Pfam	30.958607	No description for PF08579.2 was found. No confident structure predictions are available.
3	View Details	[240..332]	FFAS03	1.06	The superhelical TPR domain of O-linked GlcNAc transferase reveals structural similarities to importin alpha.
4	View Details	[333..854]	FFAS03	1.08	No description for 2gw1A was found.
5	View Details	[855..1084]	deduced	N/A	No confident structure predictions are available.
6	View Details	[1085..1202]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2