Protein Overview: RPS31

General Information:
Name(s) found:	RPS31 / YLR167W [SGD]
Description(s) found: Found 25 descriptions. SHOW ALL	Fusion protein that is cleaved to yield a ribosomal protein of the small (40S) subunit and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B [SGD]
Organism:	Saccharomyces cerevisiae
Length:	152 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA]
Biological Process:	ribosome biogenesis [IDA] ribosomal small subunit assembly [IMP] translation [IDA] protein ubiquitination [IMP]
Molecular Function:	structural constituent of ribosome [IDA] protein tag [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN  60
   61 IQKESTLHLV LRLRGGGKKR KKKVYTTPKK IKHKHKKVKL AVLSYYKVDA EGKVTKLRRE 120
  121 CSNPTCGAGV FLANHKDRLY CGKCHSVYKV NA

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		ASC1 RPS0A RPS0B RPS10A RPS10B RPS11A, RPS11B RPS12 RPS13 RPS14A RPS14B RPS15 RPS16B, RPS16A RPS17A RPS17B RPS18B, RPS18A RPS19A RPS19B RPS1A RPS1B RPS2 RPS20 RPS21A RPS21B RPS22A RPS22B RPS23B, RPS23A RPS24A, RPS24B RPS25A RPS25B RPS26A RPS26B RPS27A RPS27B RPS28A RPS28B RPS29A RPS29B RPS3 RPS30B, RPS30A RPS31 RPS4B, RPS4A RPS5 RPS6A, RPS6B RPS7A RPS7B RPS8B, RPS8A RPS9A RPS9B
View Details	Krogan NJ, et al. (2006)		CWP2 DBP2 FLC2 PPN1 RPS31 SRP40 TFA1 TFA2 YAP1801
View Details	Qiu et al. (2008)		ASC1 ATG27 FYV12 NAT3 RKI1 RPL10 RPL11A RPL11B RPL12B, RPL12A RPL13A RPL13B RPL14A RPL15A RPL15B RPL16A RPL16B RPL17A RPL17B RPL18A, RPL18B RPL19B, RPL19A RPL1A, RPL1B RPL20A RPL20B RPL21A RPL21B RPL22A RPL23B, RPL23A RPL24A RPL24B RPL25 RPL26A RPL26B RPL27B RPL28 RPL29 RPL2A, RPL2B RPL3 RPL30 RPL31A RPL31B RPL32 RPL33A RPL33B RPL34A RPL35A, RPL35B RPL36A RPL36B RPL37A RPL37B RPL38 RPL39 RPL40B, RPL40A RPL41A, RPL41B RPL42B, RPL42A RPL43B, RPL43A RPL4A RPL5 RPL6A RPL6B RPL7A RPL7B RPL8A RPL8B RPL9A RPL9B RPP0 RPP1A RPP1B RPP2A RPP2B RPS0A RPS0B RPS10A RPS10B RPS11A, RPS11B RPS12 RPS13 RPS14A RPS14B RPS15 RPS16B, RPS16A RPS17A RPS17B RPS18B, RPS18A RPS19A RPS19B RPS1A RPS1B RPS2 RPS20 RPS21A RPS21B RPS22A RPS22B RPS23B, RPS23A RPS24A, RPS24B RPS25A RPS25B RPS26A RPS26B RPS27A RPS27B RPS28A RPS28B RPS29A RPS29B RPS3 RPS30B, RPS30A RPS31 RPS4B, RPS4A RPS5 RPS6A, RPS6B RPS7A RPS7B RPS8B, RPS8A RPS9A RPS9B TEF1, TEF2 TSR1 VAR1 YML6

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	LST8	No Comments	Reinke A, et al. (2004)
View Run	BIR1	No Comments	Widlund PO, et al. (2005)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	VAM3	sample: hx4	Hao Xu, et al. (2010)
View Run	VAM3	hx23: mixture in detergent (control) Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex	Hao Xu, et al. (2010)
View Run	MLP2	#19 Asynchronous Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#28 Asynchronous Prep5-TiO2 Phosphopeptide Enrichment	Keck JM, et al. (2011)
View Run	MLP2	#19b Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#05 Alpha Factor Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#07 Alpha Factor Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#09 Alpha Factor Prep4-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#19a Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RAD4	RPS31	2	Uetz P, et al. (2000)
View Screen	UBC7	RPS31	2	Unpublished Fields Lab Data
View Screen	CDC13	RPS31	1	Unpublished Fields Lab Data
View Screen	SDS23	RPS31	1	Unpublished Fields Lab Data
View Screen	RRB1	RPS31	1	Unpublished Fields Lab Data
View Screen	RIM11	RPS31	1	Unpublished Fields Lab Data
View Screen	UBC11	RPS31	1	Unpublished Fields Lab Data
View Screen	ILM1	RPS31	1	Lockshon D, et al. (2006)

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..89]	PSI-BLAST	378.071898	Ubiquitin
2	View Details	[90..152]	Pfam	31.124939	Ribosomal protein S27a No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2