Protein Overview: GCN2

General Information:
Name(s) found:	GCN2 / YDR283C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6320489\|ref\|NP_010569.1\| Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; Gcn2p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|OKBYN2 protein kinase GCN2 (EC 2.7.1.-) - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1332634\|gb\|AAB64461.1\| Gcn2p: Protein kinase, phosphorylates the alpha subunit of eIF-2 (Swiss prot. accession number P15442). Note that this protein is lon [Saccharomyces cerevisiae] [NCBI NR] gi\|114152808\|sp\|P15442.3\|GCN2_YEAST RecName: Full=Serine/threonine-protein kinase GCN2 [NCBI NR] Serine/threonine-protein kinase GCN2 OS=Saccharomyces cerevisiae GN=GCN2 PE=1 SV=3 [Swiss-Prot] GCN2 Ser/thr protein kinase [mips] Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control [SGD] GCN2, Chr IV from 1025061-1030040, reverse complement [yeast_orfs2] Chr IV from 1025061-1030040, reverse complement [yeastorf3.fasta] Chr IV from 1030040-1025061, reverse complement [yeastorf5.fasta] GCN2 SGDID:S0002691, Chr IV from 1030041-1025062, reverse complement, Verified ORF [yeastflysequest.fasta] GCN2 SGDID:S0002691, Chr IV from 1030043-1025064, reverse complement, Verified ORF [10112005cervSGD.fasta] Gcn2p [Saccharomyces cerevisiae]gi\|2144426\|pir\|\|OKBYN2 protein kinase GCN2 (EC 2.7.1.-) - yeast (Saccharomyces cerevisiae)gi\|1332634\|gb\|AAB64461.1\| Gcn2p: Protein kinase, phosphorylates the alpha subunit of eIF-2 (Swiss prot. accession number P15442). [nrdb_11152004_Chlamydomonas] GCN2 SGDID:S000002691, Chr IV from 1030043-1025064, reverse complement, Verified ORF, "Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] GCN2 SGDID:S000002691, Chr IV from 1030045-1025066, reverse complement, Verified ORF, "Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn [yeast-contam.fasta] GCN2 SGDID:S000002691, Chr IV from 1030046-1025067, reverse complement, Verified ORF, "Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; Gcn2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Gcn2p: Protein kinase, phosphorylates the alpha subunit of eIF-2 (Swiss prot. accession number P15442). Note that this protein is lon [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Serine/threonine-protein kinase GCN2 [nr-271106-contam.fasta] GCN2 SGDID:S000002691, Chr IV from 1030046-1025067, reverse complement, Verified ORF, "Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control" [yeast-bovrabprots.fasta] GCN2 SGDID:S000002691, Chr IV from 1030045-1025066, reverse complement, Verified ORF, "Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex" [yeast-contam_custom.fasta] [chemostatdb.fasta] Serine/threonine-protein kinase GCN2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GCN2 PE=1 SV=3 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] GCN2 SGDID:S000002691, Chr IV from 1030049-1025070, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control" [yeast-030211-contam.fasta] GCN2 SGDID:S000002691, Chr IV from 1030049-1025070, Genome Release 64-2-1, reverse complement, Verified ORF, "Protein kinase; phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control" [20141208_orf_trans.fasta] YDR283C SGDID:S000002691, Chr IV from 1030049-1025070, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] eIF-2-alpha kinase GCN2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GCN2 PE=1 SV=3 [uniprot_sprot.fasta.20160308]
Organism:	Saccharomyces cerevisiae
Length:	1659 amino acids
Gene Ontology:
Cellular Component:	cytosolic ribosome [IDA]
Biological Process:	cellular amino acid biosynthetic process [IMP] regulation of translational initiation [IDA] protein amino acid phosphorylation [IDA]
Molecular Function:	protein kinase activity [IDA] eukaryotic translation initiation factor 2alpha kinase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSLSHLTLDQ YYEIQCNELE AIRSIYMDDF TDLTKRKSSW DKQPQIIFEI TLRSVDKEPV  60
   61 ESSITLHFAM TPMYPYTAPE IEFKNVQNVM DSQLQMLKSE FKKIHNTSRG QEIIFEITSF 120
  121 TQEKLDEFQN VVNTQSLEDD RLQRIKETKE QLEKEEREKQ QETIKKRSDE QRRIDEIVQR 180
  181 ELEKRQDDDD DLLFNRTTQL DLQPPSEWVA SGEAIVFSKT IKAKLPNNSM FKFKAVVNPK 240
  241 PIKLTSDIFS FSKQFLVKPY IPPESPLADF LMSSEMMENF YYLLSEIELD NSYFNTSNGK 300
  301 KEIANLEKEL ETVLKAKHDN VNRLFGYTVE RMGRNNATFV WKIRLLTEYC NYYPLGDLIQ 360
  361 SVGFVNLATA RIWMIRLLEG LEAIHKLGIV HKCINLETVI LVKDADFGST IPKLVHSTYG 420
  421 YTVLNMLSRY PNKNGSSVEL SPSTWIAPEL LKFNNAKPQR LTDIWQLGVL FIQIISGSDI 480
  481 VMNFETPQEF LDSTSMDETL YDLLSKMLNN DPKKRLGTLE LLPMKFLRTN IDSTINRFNL 540
  541 VSESVNSNSL ELTPGDTITV RGNGGRTLSQ SSIRRRSFNV GSRFSSINPA TRSRYASDFE 600
  601 EIAVLGQGAF GQVVKARNAL DSRYYAIKKI RHTEEKLSTI LSEVMLLASL NHQYVVRYYA 660
  661 AWLEEDSMDE NVFESTDEES DLSESSSDFE ENDLLDQSSI FKNRTNHDLD NSNWDFISGS 720
  721 GYPDIVFENS SRDDENEDLD HDTSSTSSSE SQDDTDKESK SIQNVPRRRN FVKPMTAVKK 780
  781 KSTLFIQMEY CENRTLYDLI HSENLNQQRD EYWRLFRQIL EALSYIHSQG IIHRDLKPMN 840
  841 IFIDESRNVK IGDFGLAKNV HRSLDILKLD SQNLPGSSDN LTSAIGTAMY VATEVLDGTG 900
  901 HYNEKIDMYS LGIIFFEMIY PFSTGMERVN ILKKLRSVSI EFPPDFDDNK MKVEKKIIRL 960
  961 LIDHDPNKRP GARTLLNSGW LPVKHQDEVI KEALKSLSNP SSPWQQQVRE SLFNQSYSLT1020
 1021 NDILFDNSVP TSTPFANILR SQMTEEVVKI FRKHGGIENN APPRIFPKAP IYGTQNVYEV1080
 1081 LDKGGTVLQL QYDLTYPMAR YLSKNPSLIS KQYRMQHVYR PPDHSRSSLE PRKFGEIDFD1140
 1141 IISKSSSESG FYDAESLKII DEILTVFPVF EKTNTFFILN HADILESVFN FTNIDKAQRP1200
 1201 LVSRMLSQVG FARSFKEVKN ELKAQLNISS TALNDLELFD FRLDFEAAKK RLYKLMIDSP1260
 1261 HLKKIEDSLS HISKVLSYLK PLEVARNVVI SPLSNYNSAF YKGGIMFHAV YDDGSSRNMI1320
 1321 AAGGRYDTLI SFFARPSGKK SSNTRKAVGF NLAWETIFGI AQNYFKLASG NRIKKRNRFL1380
 1381 KDTAVDWKPS RCDVLISSFS NSLLDTIGVT ILNTLWKQNI KADMLRDCSS VDDVVTGAQQ1440
 1441 DGIDWILLIK QQAYPLTNHK RKYKPLKIKK LSTNVDIDLD LDEFLTLYQQ ETGNKSLIND1500
 1501 SLTLGDKADE FKRWDENSSA GSSQEGDIDD VVAGSTNNQK VIYVPNMATR SKKANKREKW1560
 1561 VYEDAARNSS NMILHNLSNA PIITVDALRD ETLEIISITS LAQKEEWLRK VFGSGNNSTP1620
 1621 RSFATSIYNN LSKEAHKGNR WAILYCHKTG KSSVIDLQR

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Gavin AC, et al. (2006)		AAR2 GCN2 LTV1 RPM2 SNU66
View Details	Gavin AC, et al. (2002)		AAR2 BRR1 BUD31 CDC33 CEF1 CLF1 CSN12 CUS1 CWC2 CWC22 CWC23 DHH1 DIB1 ECM2 GCN2 HSH155 HTA1 IMG1 LEA1 LIN1 LSM1 LSM2 LSM4 LSM5 LSM6 LSM7 LUC7 MRP7 MRPL28 MRPL3 MRPL35 MRPL38 MRPL4 MRPL8 MRPS5 MSL1 MUD1 PAT1 PRP11 PRP18 PRP19 PRP21 PRP24 PRP28 PRP3 PRP31 PRP38 PRP39 PRP4 PRP40 PRP42 PRP43 PRP46 PRP6 PRP8 PRP9 RSE1 RVB2 SLU7 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNP1 SNT309 SNU114 SNU23 SNU56 SNU66 SNU71 SPP381 SPP382 SRB2 STO1 SYF1 TOS4 UTP21 YML6
View Details	Ho Y, et al. (2002)		GCN2 RRG9

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..199]	MSA	1.054963	View MSA. No confident structure predictions are available.
2	View Details	[200..263]	deduced	N/A	Confident ab initio structure predictions are available.
3	View Details	[264..579]	PSI-BLAST	160.626727	MAP kinase Erk2
4	View Details	[580..702]	PSI-BLAST	57.201249	No description for 1lwpA_ was found.
5	View Details	[703..800]	PSI-BLAST	57.201249	No description for 1lwpA_ was found.
6	View Details	[801..938]	PSI-BLAST	57.201249	No description for 1lwpA_ was found.
7	View Details	[939..1029]	PSI-BLAST	271.0	Calmodulin-dependent protein kinase
8	View Details	[1030..1062] [1093..1117] [1134..1371]	PSI-BLAST	16.0	Histidyl-tRNA synthetase (HisRS), C-terminal domain; Histidyl-tRNA synthetase (HisRS)
9	View Details	[1063..1092] [1118..1133] [1372..1517]	PSI-BLAST	16.0	Histidyl-tRNA synthetase (HisRS), C-terminal domain; Histidyl-tRNA synthetase (HisRS)
10	View Details	[1518..1659]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
ligase activity	5.77046276233401	bayes_pls_golite062009
small conjugating protein ligase activity	3.65045022869763	bayes_pls_golite062009
ubiquitin-protein ligase activity	3.38582942748827	bayes_pls_golite062009
eukaryotic translation initiation factor 2alpha kinase activity	3.23512529981344	bayes_pls_golite062009
acid-amino acid ligase activity	2.62286563200644	bayes_pls_golite062009
ligase activity, forming aminoacyl-tRNA and related compounds	2.4573066461497	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	2.4573066461497	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	2.44492491338365	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	1.69002970237151	bayes_pls_golite062009
kinase activity	1.63748693386705	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	1.47781099411385	bayes_pls_golite062009
binding	1.32417336730245	bayes_pls_golite062009
protein kinase activity	1.24946862508965	bayes_pls_golite062009
transferase activity	1.12000675735902	bayes_pls_golite062009
nucleic acid binding	1.07247161465191	bayes_pls_golite062009
transcription regulator activity	1.07160795075065	bayes_pls_golite062009
protein serine/threonine kinase activity	0.972449791105909	bayes_pls_golite062009
DNA binding	0.933974549145269	bayes_pls_golite062009
catalytic activity	0.891402822028799	bayes_pls_golite062009
protein binding	0.726328463755484	bayes_pls_golite062009
ligase activity, forming carbon-nitrogen bonds	0.509761707991986	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
phosphotransferase activity, alcohol group as acceptor	5.49988294224815	bayes_pls_golite062009
kinase activity	5.3287080791198	bayes_pls_golite062009
protein kinase activity	5.17195961442593	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	4.96052051118534	bayes_pls_golite062009
protein serine/threonine kinase activity	4.46381887831481	bayes_pls_golite062009
transferase activity	3.97138602499442	bayes_pls_golite062009
structural molecule activity	3.82327979736517	bayes_pls_golite062009
ligase activity, forming aminoacyl-tRNA and related compounds	2.33015238923914	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	2.33015238923914	bayes_pls_golite062009
binding	2.31930216202447	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	2.31770167474216	bayes_pls_golite062009
ligase activity	2.15844649250746	bayes_pls_golite062009
ATP binding	1.97600611746091	bayes_pls_golite062009
adenyl ribonucleotide binding	1.93596671856664	bayes_pls_golite062009
adenyl nucleotide binding	1.90928021591891	bayes_pls_golite062009
purine ribonucleotide binding	1.65732943334212	bayes_pls_golite062009
ribonucleotide binding	1.65726906484847	bayes_pls_golite062009
purine nucleotide binding	1.63763394711025	bayes_pls_golite062009
nucleotide binding	1.63195828048121	bayes_pls_golite062009
protein tyrosine kinase activity	1.3898059094397	bayes_pls_golite062009
receptor signaling protein serine/threonine kinase activity	1.38496328649031	bayes_pls_golite062009
receptor signaling protein activity	1.27291233344322	bayes_pls_golite062009
protein binding	1.22565748038754	bayes_pls_golite062009
catalytic activity	1.21655087018101	bayes_pls_golite062009
eukaryotic translation initiation factor 2alpha kinase activity	1.09810837428564	bayes_pls_golite062009
protein serine/threonine/tyrosine kinase activity	0.48345772948903	bayes_pls_golite062009
transcription regulator activity	0.425540150205164	bayes_pls_golite062009
cyclin-dependent protein kinase activity	0.39973598464632	bayes_pls_golite062009
nucleic acid binding	0.398242700677703	bayes_pls_golite062009
magnesium ion binding	0.393328812780268	bayes_pls_golite062009
MAP kinase kinase kinase activity	0.37115026509016	bayes_pls_golite062009
MAP kinase kinase activity	0.35402045013148	bayes_pls_golite062009
DNA binding	0.331341423430082	bayes_pls_golite062009
signal transducer activity	0.270054246024761	bayes_pls_golite062009
molecular transducer activity	0.270054246024761	bayes_pls_golite062009
RNA binding	0.245748631571068	bayes_pls_golite062009
transcription factor activity	0.151610605304256	bayes_pls_golite062009
transmembrane receptor protein kinase activity	0.0556163711947139	bayes_pls_golite062009
transmembrane receptor activity	0.00652065412910241	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
ligase activity, forming aminoacyl-tRNA and related compounds	7.89468709683036	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	7.89468709683036	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	7.88410036819636	bayes_pls_golite062009
ligase activity	6.92073480193262	bayes_pls_golite062009
histidine-tRNA ligase activity	5.91343220471745	bayes_pls_golite062009
phenylalanine-tRNA ligase activity	3.22631630581238	bayes_pls_golite062009
glycine-tRNA ligase activity	2.29229136935761	bayes_pls_golite062009
threonine-tRNA ligase activity	2.18642354927	bayes_pls_golite062009
serine-tRNA ligase activity	2.07594942154162	bayes_pls_golite062009
catalytic activity	1.99372273395202	bayes_pls_golite062009
alanine-tRNA ligase activity	1.82594054955281	bayes_pls_golite062009
lysine-tRNA ligase activity	1.72324031407565	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	1.43493514551266	bayes_pls_golite062009
binding	1.11470390774037	bayes_pls_golite062009
transferase activity	1.00944766864901	bayes_pls_golite062009
transcription regulator activity	0.936096346913383	bayes_pls_golite062009
asparagine-tRNA ligase activity	0.921492123574877	bayes_pls_golite062009
nucleic acid binding	0.817940471445751	bayes_pls_golite062009
ATP binding	0.78849239804069	bayes_pls_golite062009
DNA binding	0.755075958424431	bayes_pls_golite062009
adenyl ribonucleotide binding	0.751370849162632	bayes_pls_golite062009
adenyl nucleotide binding	0.727917585848449	bayes_pls_golite062009
RNA binding	0.576453255038334	bayes_pls_golite062009
purine ribonucleotide binding	0.482944943143156	bayes_pls_golite062009
ribonucleotide binding	0.48293725802639	bayes_pls_golite062009
purine nucleotide binding	0.467088940808932	bayes_pls_golite062009
nucleotide binding	0.457991109108443	bayes_pls_golite062009
transcription factor activity	0.151651763713257	bayes_pls_golite062009
aspartate-tRNA ligase activity	0.00572514744734753	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

	PROTEIN(S)	PUBLICATION
View Data	GCN2	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Protein Overview: GCN2

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: