Protein Overview: CDC15

General Information:
Name(s) found:	CDC15 / YAR019C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6319328\|ref\|NP_009411.1\| Protein kinase of the Mitotic Exit Network that is localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; Cdc15p [Saccharomyces cerevisiae] [NCBI NR] gi\|349757\|gb\|AAC04965.1\| Cdc15p: Putative kinase [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S15038 cell division control protein CDC15 - yeast (Saccharomyces cerevisiae) [NCBI NR] CDC15 Protein kinase of the MAP kinase kinase kinase family [mips] Protein kinase of the Mitotic Exit Network that is localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II [SGD] CDC15, Chr I from 172205-175129, reverse complement [yeast_orfs2] Chr I from 172205-175129, reverse complement [yeastorf3.fasta] Chr I from 172209-175133, reverse complement [yeastorf4.fasta] Chr I from 175133-172209, reverse complement [yeastorf5.fasta] CDC15 SGDID:S0000072, Chr I from 175135-172211, reverse complement, Verified ORF [yeastflysequest.fasta] CDC15 SGDID:S0000072, Chr I from 175133-172209, reverse complement, Verified ORF [10112005cervSGD.fasta] Cdc15p [Saccharomyces cerevisiae]gi\|101245\|pir\|\|S15038 cell division control protein CDC15 - yeast (Saccharomyces cerevisiae)gi\|349757\|gb\|AAC04965.1\| Cdc15p: Putative kinase [Saccharomyces cerevisiae] [nrdb_11152004_Chlamydomonas] CDC15 SGDID:S000000072, Chr I from 175133-172209, reverse complement, Verified ORF, "Protein kinase of the Mitotic Exit Network that is localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activi [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Cdc15p: Putative kinase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Protein kinase of the Mitotic Exit Network that is localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; Cdc15p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] CDC15 SGDID:S000000072, Chr I from 175133-172209, reverse complement, Verified ORF, "Protein kinase of the Mitotic Exit Network that is localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II" [yeast-bovrabprots.fasta] CDC15 SGDID:S000000072, Chr I from 175133-172209, reverse complement, Verified ORF, "Protein kinase of the Mitotic Exit Network that is localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p" [yeast-contam_custom.fasta] [chemostatdb.fasta]
Organism:	Saccharomyces cerevisiae
Length:	974 amino acids
Gene Ontology:
Cellular Component:	cellular bud neck [TAS] spindle pole body [IDA]
Biological Process:	regulation of exit from mitosis [IMP] cytokinesis [IMP] protein amino acid phosphorylation [IDA]
Molecular Function:	protein kinase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNSMADTDRV NLTPIQRASE KSVQYHLKQV IGRGSYGVVY KAINKHTDQV VAIKEVVYEN  60
   61 DEELNDIMAE ISLLKNLNHN NIVKYHGFIR KSYELYILLE YCANGSLRRL ISRSSTGLSE 120
  121 NESKTYVTQT LLGLKYLHGE GVIHRDIKAA NILLSADNTV KLADFGVSTI VNSSALTLAG 180
  181 TLNWMAPEIL GNRGASTLSD IWSLGATVVE MLTKNPPYHN LTDANIYYAV ENDTYYPPSS 240
  241 FSEPLKDFLS KCFVKNMYKR PTADQLLKHV WINSTENVKV DKLNKFKEDF TDADYHWDAD 300
  301 FQEEKLNISP SKFSLRAAPA PWAENNQELD LMPPTESQLL SQLKSSSKPL TDLHVLFSVC 360
  361 SLENIADTII ECLSRTTVDK RLITAFGSIF VYDTQHNHSR LRLKFIAMGG IPLIIKFEHL 420
  421 AKEFVIDYPQ TLIECGIMYP PNFASLKTPK YILELVYRFY DLTSTAFWCR WCFKHLDISL 480
  481 LLNNIHERRA QSILLKLSSY APWSFEKILP SLIDSKLKKK ILISPQITYV VFKSINYMIT 540
  541 TNDDKIHKSA IPSSSSLPLS SSPTRNSPVN SVQSPSRSPV HSLMATRPSS PMRHKSISNF 600
  601 PHLTISSKSR LLIELPEGFF TWLTSFFVDM AQIKDLSVLK YFTKLCYLTV HINSTFLNDL 660
  661 LDNDAFFAFI RNIDTIIPFI DDAKTAAFIW KQITAICVEM SLDMDQMSAS LFSTAMNFIR 720
  721 KKNNTSISGL EIILNCLHFT LRNVNDDVAP TVGSSESHSV FLIKVNNDAA IELPIDQLVD 780
  781 LFYALNDDDV NLSKLISIFT KICSLPGFEN LTINIIFHPN FYEKIVSFFD TYFNSLLIQI 840
  841 DLLKFIKLIF SKSLLKLYDY TGQPDPIKQT EPNRRNKATV FKLRAILVQI TEFLNNNWNN 900
  901 GCPKRNSNQV GGDSVLICQL CEDIRSLSKK GSLQKVSSVT AAIGSSPTKD ERSNLRSSKD 960
  961 KSDGFSVPIT TFQT

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CDC15 DUG2 TEM1
View Details	Krogan NJ, et al. (2006)		BUD4 CDC13 CDC15 GIR2 PRS5 RBG2 YIL152W
View Details	Ho Y, et al. (2002)		ATG4 BFA1 CAR2 CDC15 CDC33 CLU1 COF1 COR1 CPA2 CPR1 CPR3 CYS4 DBP8 DUG2 DUT1 FAA4 GCD11 GUS1 HEF3 KGD1 MCX1 NMD3 NUP53 OYE2 PFK1 PGM2 PST2 RNR1 RNR2 RPN1 SAR1 SEC26 SEC53 SSD1 TEF4 TEM1 TFP1 THI22 TIF1, TIF2 TMA29 UFD4 YDL086W YGR066C YHR033W YNK1 YTM1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	UBC7	CDC15	1	Unpublished Fields Lab Data
View Screen	TOS3	CDC15	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	CDC15 and SPC42	Huh WK, et al. (2003)
View Data	CDC15	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..101]	PSI-BLAST	447.218487	pak1
2	View Details	[102..347]	PSI-BLAST	447.218487	pak1
3	View Details	[348..864]	deduced	N/A	No confident structure predictions are available.
4	View Details	[865..974]	MSA	1.002947	View MSA. No confident structure predictions are available.
5	View Details	[758..871]	deduced	N/A	No confident structure predictions are available.
6	View Details	[872..974]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
phosphotransferase activity, alcohol group as acceptor	5.49988294224815	bayes_pls_golite062009
protein kinase activity	5.4586557943719	bayes_pls_golite062009
kinase activity	5.3287080791198	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	4.96052051118534	bayes_pls_golite062009
protein serine/threonine kinase activity	4.46381887831481	bayes_pls_golite062009
calmodulin-dependent protein kinase activity	4.31938467136214	bayes_pls_golite062009
transferase activity	3.97138602499442	bayes_pls_golite062009
binding	2.74863538127007	bayes_pls_golite062009
protein binding	2.276111749106	bayes_pls_golite062009
tubulin binding	2.18641675163967	bayes_pls_golite062009
cytoskeletal protein binding	2.12615078788254	bayes_pls_golite062009
fibroblast growth factor receptor activity	2.07350687583102	bayes_pls_golite062009
ATP binding	1.97600611746091	bayes_pls_golite062009
adenyl ribonucleotide binding	1.93596671856664	bayes_pls_golite062009
adenyl nucleotide binding	1.90928021591891	bayes_pls_golite062009
microtubule binding	1.88110801192607	bayes_pls_golite062009
purine ribonucleotide binding	1.65732943334212	bayes_pls_golite062009
ribonucleotide binding	1.65726906484847	bayes_pls_golite062009
purine nucleotide binding	1.63763394711025	bayes_pls_golite062009
nucleotide binding	1.63195828048121	bayes_pls_golite062009
fibroblast growth factor binding	1.46046123990071	bayes_pls_golite062009
JUN kinase kinase kinase activity	1.41172362253967	bayes_pls_golite062009
protein tyrosine kinase activity	1.3898059094397	bayes_pls_golite062009
receptor signaling protein serine/threonine kinase activity	1.38496328649031	bayes_pls_golite062009
actin binding	1.33479685165255	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	1.32254739984688	bayes_pls_golite062009
gamma-tubulin binding	1.28511591054866	bayes_pls_golite062009
receptor signaling protein activity	1.27291233344322	bayes_pls_golite062009
catalytic activity	1.21655087018101	bayes_pls_golite062009
fibroblast growth factor 1 binding	1.0383359654359	bayes_pls_golite062009
cyclin-dependent protein kinase activity	0.959360414036299	bayes_pls_golite062009
MAP kinase activity	0.81943579965027	bayes_pls_golite062009
structural constituent of cytoskeleton	0.612883428421652	bayes_pls_golite062009
transporter activity	0.595111702412257	bayes_pls_golite062009
insulin receptor substrate binding	0.57867852584301	bayes_pls_golite062009
protein serine/threonine/tyrosine kinase activity	0.48345772948903	bayes_pls_golite062009
enzyme binding	0.46284745266176	bayes_pls_golite062009
transmembrane transporter activity	0.41384976209545	bayes_pls_golite062009
magnesium ion binding	0.393328812780268	bayes_pls_golite062009
MAP kinase kinase kinase activity	0.37115026509016	bayes_pls_golite062009
MAP kinase kinase activity	0.35402045013148	bayes_pls_golite062009
protein complex binding	0.343240719858581	bayes_pls_golite062009
structure-specific DNA binding	0.318153468064373	bayes_pls_golite062009
signal transducer activity	0.270054246024761	bayes_pls_golite062009
molecular transducer activity	0.270054246024761	bayes_pls_golite062009
actin filament binding	0.2403700250846	bayes_pls_golite062009
ubiquitin-protein ligase activity	0.114318824149111	bayes_pls_golite062009
substrate-specific transporter activity	0.0922946791401962	bayes_pls_golite062009
transmembrane receptor protein kinase activity	0.0556163711947139	bayes_pls_golite062009
transmembrane receptor activity	0.00652065412910241	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.0040394359628233	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2