Protein Overview: SIS1

General Information:
Name(s) found:	SIS1 / YNL007C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324321\|ref\|NP_014391.1\| Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; shares similarity with bacterial DnaJ proteins; Sis1p [Saccharomyces cerevisiae] [NCBI NR] gi\|4474\|emb\|CAA41366.1\| SIS1 protein [Saccharomyces cerevisiae] [NCBI NR] gi\|134509\|sp\|P25294.1\|SIS1_YEAST RecName: Full=Protein SIS1 [NCBI NR] gi\|1301824\|emb\|CAA95866.1\| SIS1 [Saccharomyces cerevisiae] [NCBI NR] pir\|\|A39660 heat shock protein SIS1 - yeast (Saccharomyces cerevisiae) [NCBI NR] Protein SIS1 OS=Saccharomyces cerevisiae GN=SIS1 PE=1 SV=1 [Swiss-Prot] SIS1 Heat shock protein [mips] Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; shares similarity with bacterial DnaJ proteins [SGD] SIS1, Chr XIV from 618504-619562, reverse complement [yeast_orfs2] Chr XIV from 618504-619562, reverse complement [yeastorf3.fasta] Chr XIV from 619562-618504, reverse complement [yeastorf5.fasta] (P25294) SIS1 protein [4932.FASTACCERV.txt] SIS1 SGDID:S0004952, Chr XIV from 619564-618506, reverse complement, Verified ORF [yeastflysequest.fasta] SIS1 SGDID:S0004952, Chr XIV from 619565-618507, reverse complement, Verified ORF [10112005cervSGD.fasta] HSP40 family chaperone; sit4 suppressor, dnaJ homolog [Saccharomyces cerevisiae]gi\|1301824\|emb\|CAA95866.1\| SIS1 [Saccharomyces cerevisiae]gi\|4474\|emb\|CAA41366.1\| SIS1 protein [Saccharomyces cerevisiae]gi\|101623\|pir\|\|A39660 heat shock protein SIS1 - yea [nrdb_11152004_Chlamydomonas] SIS1 SGDID:S000004952, Chr XIV from 619567-618509, reverse complement, Verified ORF, "Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; shares similarity with [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Protein SIS1 [nr-271106-contam.fasta] SIS1 protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; shares similarity with bacterial DnaJ proteins; Sis1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] SIS1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] SIS1 SGDID:S000004952, Chr XIV from 619568-618510, reverse complement, Verified ORF, "Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; shares similarity with [SGD_S-cerevisiae_na_01-05-2010_reversed.fasta] SIS1 SGDID:S000004952, Chr XIV from 619567-618509, reverse complement, Verified ORF, "Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; shares similarity with bacterial DnaJ proteins" [yeast-bovrabprots.fasta] [chemostatdb.fasta] SIS1 SGDID:S000004952, Chr XIV from 619568-618510, reverse complement, Verified ORF, "Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; shares similarity with bacterial DnaJ proteins" [SGDyeast.20101221.fasta] Protein SIS1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SIS1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SIS1 SGDID:S000004952, Chr XIV from 619565-618507, Genome Release 64-1-1, reverse complement, Verified ORF, "Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; shares similarity with bacterial DnaJ proteins" [yeast-030211-contam.fasta] SIS1 SGDID:S000004952, Chr XIV from 619565-618507, Genome Release 64-2-1, reverse complement, Verified ORF, "Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; shuttles between cytosol and nucleus; mediates delivery of misfolded proteins into the nucleus for degradation; involved in proteasomal degradation of misfolded cytosolic proteins; protein abundance increases in response to DNA replication stress; polyQ aggregates sequester Sis1p and interfere with clearance of misfolded proteins; similar to bacterial DnaJ proteins and mammalian DnaJB1" [20141208_orf_trans.fasta] YNL007C SGDID:S000004952, Chr XIV from 619565-618507, Genome Release 64-1-1, reverse complement, Verified ORF, "Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; shares similarity with bacterial DnaJ proteins" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	352 amino acids
Gene Ontology:
Cellular Component:	nucleus [IEA] cytoplasm [IEA] cytosolic small ribosomal subunit [IDA]
Biological Process:	translational initiation [IMP] protein folding [IDA][IMP]
Molecular Function:	unfolded protein binding [TAS] chaperone regulator activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVKETKLYDL LGVSPSANEQ ELKKGYRKAA LKYHPDKPTG DTEKFKEISE AFEILNDPQK  60
   61 REIYDQYGLE AARSGGPSFG PGGPGGAGGA GGFPGGAGGF SGGHAFSNED AFNIFSQFFG 120
  121 GSSPFGGADD SGFSFSSYPS GGGAGMGGMP GGMGGMHGGM GGMPGGFRSA SSSPTYPEEE 180
  181 TVQVNLPVSL EDLFVGKKKS FKIGRKGPHG ASEKTQIDIQ LKPGWKAGTK ITYKNQGDYN 240
  241 PQTGRRKTLQ FVIQEKSHPN FKRDGDDLIY TLPLSFKESL LGFSKTIQTI DGRTLPLSRV 300
  301 QPVQPSQTST YPGQGMPTPK NPSQRGNLIV KYKVDYPISL NDAQKRAIDE NF

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Gavin AC, et al. (2002)		CDC24 HSP42 SIS1
View Details	Ho Y, et al. (2002)		AHA1 ARP2 ATP3 AVO1 BCK1 CBK1 CCT5 CDC53 CLU1 CPR6 DHH1 DPB2 EGD2 FOL2 FUN12 GAL7 GND1 GPH1 IDH1 ILV2 ILV5 IPP1 KIC1 KIN2 LHS1 LYS12 MET16 MET30 MET31 MET4 MKK2 MKT1 MOB2 NOG2 NPA3 OYE2 PDC6 PDX1 PHO85 PIL1 PMA1 PUF3 QCR2 RCN2 RPN12 RPN6 RPT3 RUB1 SEC28 SIS1 SKG3 SKP1 SLT2 SLX1 SMK1 SRP54 STI1 TAO3 TEF4 TEM1 TFC7 TIF1, TIF2 VMA6 VMA8 YER077C YGR266W YKR051W YKU80 YLR271W YPR003C

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	SIS1	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..79]	PSI-BLAST	188.221849	DnaJ chaperone, N-terminal (J) domain
2	View Details	[80..180]	ORFEUS	7.38	No description for 1la5A was found.
3	View Details	[181..257]	PSI-BLAST	937.512579	Heat shock protein 40 Sis1
4	View Details	[258..352]	PSI-BLAST	937.512579	Heat shock protein 40 Sis1

Protein Overview: SIS1

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: