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Protein Overview: RPL8B

General Information:
Name(s) found: RPL8B / YLL045C [SGD]
Description(s) found:
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  • gi|6322984|ref|NP_013055.1| Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Ap and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits; Rpl8bp [Saccharomyces cerevisiae [NCBI NR]
  • gi|417670|sp|P29453.3|RL8B_YEAST RecName: Full=60S ribosomal protein L8-B; AltName: Full=L4; AltName: Full=L4-1; AltName: Full=YL5; AltName: Full=RP6 [NCBI NR]
  • gi|3869|emb|CAA40165.1| ribosomal protein L4-1 [Saccharomyces cerevisiae] [NCBI NR]
  • gi|1360241|emb|CAA97495.1| RPL4B [Saccharomyces cerevisiae] [NCBI NR]
  • pir||S16810 ribosomal protein L7a.e.B, cytosolic - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • 60S ribosomal protein L8-B OS=Saccharomyces cerevisiae GN=RPL8B PE=1 SV=3 [Swiss-Prot]
  • RPL8B 60s large subunit ribosomal protein L7a.e.B [mips]
  • Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Ap and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits [SGD]
  • RPL8B, Chr XII from 47858-48628, reverse complement [yeast_orfs2]
  • Chr XII from 47858-48628, reverse complement [yeastorf3.fasta]
  • Chr XII from 48628-47858, reverse complement [yeastorf5.fasta]
  • RPL8B SGDID:S0003968, Chr XII from 48628-47858, reverse complement, Verified ORF [yeastflysequest.fasta]
  • Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Ap and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits [Saccharomyces cerevisiae]gi|1360241|emb|CAA97495.1| RPL4B [ [nrdb_11152004_Chlamydomonas]
  • RPL8B SGDID:S000003968, Chr XII from 48628-47858, reverse complement, Verified ORF, "Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Ap and has similarity to rat L7a ribosomal protein; mutation results in decreased amoun [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • RPL4B [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • 60S ribosomal protein L8-B (L7A-1) (L4-1) (YL5) (RP6) [nr-271106-contam.fasta]
  • ribosomal protein L4-1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Ap and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits; Rpl8bp [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • RPL8B SGDID:S000003968, Chr XII from 48628-47858, reverse complement, Verified ORF, "Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Ap and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits" [yeast-bovrabprots.fasta]
  • [chemostatdb.fasta]
  • 60S ribosomal protein L8-B OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPL8B PE=1 SV=3 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • RPL8B SGDID:S000003968, Chr XII from 48629-47859, Genome Release 64-1-1, reverse complement, Verified ORF, "Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Ap and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits" [yeast-030211-contam.fasta]
  • RPL8B SGDID:S000003968, Chr XII from 48629-47859, Genome Release 64-2-1, reverse complement, Verified ORF, "Ribosomal 60S subunit protein L8B; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; L8 binds to Domain I of 25S and 5.8 S rRNAs; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication" [20141208_orf_trans.fasta]
  • YLL045C SGDID:S000003968, Chr XII from 48629-47859, Genome Release 64-1-1, reverse complement, Verified ORF, "Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Ap and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 256 amino acids

    Gene Ontology:
    Cellular Component: ribonucleoprotein complex [IEA]
    cytoplasm [IEA]
    cytosolic large ribosomal subunit [TAS]
    ribosome [IEA]
    Biological Process: translation [TASLink to PubMed article]
    Molecular Function: structural constituent of ribosome [TASLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAPGKKVAPA PFGAKSTKSN KAKNPLTHST PKNFGIGQAV QPKRNLSRYV KWPEYVRLQR  60
   61 QKKILSIRLK VPPTIAQFQY TLDRNTAAET FKLFNKYRPE TAAEKKERLT KEAAAIAEGK 120
  121 SKQDASPKPY AVKYGLNHVV SLIENKKAKL VLIANDVDPI ELVVFLPALC KKMGVPYAII 180
  181 KGKARLGTLV NQKTSAVAAL TEVRAEDEAA LAKLVSTIDA NFADKYDEVK KHWGGGILGN 240
  241 KAQAKMDKRA KTSDSA

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) CSR1 EBP2 NHP6A NOP56 PUF4 RPF2 RPL11B RPL16A RPL16B RPL17A RPL17B RPL18A, RPL18B RPL1A, RPL1B RPL20A RPL21A RPL24A RPL29 RPL2A, RPL2B RPL3 RPL30 RPL31B RPL32 RPL36A RPL42B, RPL42A RPL4A RPL6A RPL8B RPL9A RPS11A, RPS11B RPS16B, RPS16A RPS17B RPS22A RPS26A RPS30B, RPS30A RPS8B, RPS8A RPS9B YHI9
    View Details (MIPS) Mewes HW, et al. (2004) RPL10 RPL11A RPL11B RPL12B, RPL12A RPL13A RPL13B RPL14A RPL14B RPL15A RPL15B RPL16A RPL16B RPL17A RPL17B RPL18A, RPL18B RPL19B, RPL19A RPL1A, RPL1B RPL20A RPL20B RPL21A RPL21B RPL22A RPL22B RPL23B, RPL23A RPL24A RPL24B RPL25 RPL26A RPL26B RPL27A RPL27B RPL28 RPL29 RPL2A, RPL2B RPL3 RPL30 RPL31A RPL31B RPL32 RPL33A RPL33B RPL34A RPL34B RPL35A, RPL35B RPL36A RPL36B RPL37A RPL37B RPL38 RPL39 RPL40B, RPL40A RPL41A, RPL41B RPL42B, RPL42A RPL43B, RPL43A RPL4A RPL4B RPL5 RPL6A RPL6B RPL7A RPL7B RPL8A RPL8B RPL9A RPL9B RPP0 RPP1A RPP1B RPP2A RPP2B
    View Details Qiu et al. (2008) APT1 ASC1 BUD20 MRPL50 RKI1 RPL10 RPL11A RPL11B RPL12B, RPL12A RPL13A RPL13B RPL14A RPL14B RPL15A RPL15B RPL16A RPL16B RPL17A RPL17B RPL18A, RPL18B RPL19B, RPL19A RPL1A, RPL1B RPL20A RPL20B RPL21A RPL21B RPL22A RPL22B RPL23B, RPL23A RPL24A RPL24B RPL25 RPL26A RPL26B RPL27A RPL27B RPL28 RPL29 RPL2A, RPL2B RPL3 RPL30 RPL31A RPL31B RPL32 RPL33A RPL33B RPL34A RPL34B RPL35A, RPL35B RPL36A RPL36B RPL37A RPL37B RPL38 RPL39 RPL40B, RPL40A RPL41A, RPL41B RPL42B, RPL42A RPL43B, RPL43A RPL4A RPL4B RPL5 RPL6A RPL6B RPL7A RPL7B RPL8A RPL8B RPL9A RPL9B RPP0 RPP1A RPP1B RPP2A RPP2B RPS0A RPS0B RPS10A RPS10B RPS11A, RPS11B RPS12 RPS13 RPS14A RPS14B RPS15 RPS16B, RPS16A RPS17A RPS17B RPS18B, RPS18A RPS19A RPS19B RPS1A RPS1B RPS2 RPS20 RPS21A RPS21B RPS22A RPS22B RPS23B, RPS23A RPS24A, RPS24B RPS25A RPS25B RPS26A RPS26B RPS27A RPS27B RPS28A RPS28B RPS29A RPS29B RPS3 RPS30B, RPS30A RPS31 RPS4B, RPS4A RPS5 RPS6A, RPS6B RPS7A RPS7B RPS8B, RPS8A RPS9A RPS9B RSM26 STM1 TEF1, TEF2 TOS4 TSR1

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run TOR1 No Comments Reinke A, et al. (2004)
    View Run BIR1 No Comments Widlund PO, et al. (2005)
    View Run TOR2 No Comments Reinke A, et al. (2004)
    View Run LST8 No Comments Reinke A, et al. (2004)
    View Run TOR2 No Comments Reinke A, et al. (2004)
    View Run CLB2 No Comments McCusker D, et al (2007)
    View Run SEC10 No Comments De Craene, J., et al. (2006)
    View Run BIR1 No Comments Widlund PO, et al. (2005)
    View Run BIR1 No Comments Widlund PO, et al. (2005)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run DAD1 Dam1-765 complex purified from yeast with Dad1-TAP Shimogawa MM, et al. (2006)
    View Run ASF1 3912: TAP-tagged, strain background includes hpc2(delta) Green EM, et al (2005)
    View Run RLF2 phosphorylation data - cascade search Green EM, et al (2005)
    View Run RLF2 identification data - first search in cascade Green EM, et al (2005)
    View Run VAM3 sample: hx1 Hao Xu, et al. (2010)
    View Run VAM3 sample: hx4 Hao Xu, et al. (2010)
    View Run VAM3 Sample: HX11 - both fusion and trans-SNARE complex formation take place. Hao Xu, et al. (2010)
    View Run VAM3 Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control). Hao Xu, et al. (2010)
    View Run VAM3 Sample: HX14 - mixture in detergent (control). Hao Xu, et al. (2010)
    View Run VAM3 hx23: mixture in detergent (control) Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex Hao Xu, et al. (2010)
    View Run MLP2 #24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #10 Mitotic Prep1-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #11 Mitotic Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)
    View Run MLP2 #08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data RPL8B Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..96] MSA 5.059996 View MSA. No confident structure predictions are available.
    2 View Details [97..256] PSI-BLAST 529.383689 No description for 1k5yG_ was found.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle