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Protein Overview: GCN20

General Information:
Name(s) found: GCN20 / YFR009W [SGD]
Description(s) found:
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  • gi|836764|dbj|BAA09248.1| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR]
  • gi|643479|gb|AAA75444.1| Gcn20p [Saccharomyces cerevisiae] [NCBI NR]
  • gi|51830311|gb|AAU09721.1| YFR009W [Saccharomyces cerevisiae] [NCBI NR]
  • gi|14318531|ref|NP_116664.1| Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA; Gcn20p [Saccharomyces cerevisiae] [NCBI NR]
  • pir||S56146 GCN20 protein - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • gi|1169871|sp|P43535.1|GCN20_YEAST RecName: Full=Protein GCN20 [NCBI NR]
  • Protein GCN20 OS=Saccharomyces cerevisiae GN=GCN20 PE=1 SV=1 [Swiss-Prot]
  • (P43535) GCN20 protein [Swiss-Prot]
  • GCN20 Positive effector of Gcn2p [mips]
  • Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA [SGD]
  • GCN20, Chr VI from 162481-164739 [yeast_orfs2]
  • Chr VI from 162481-164739 [yeastorf3.fasta]
  • Chr VI from 162482-164740 [yeastorf5.fasta]
  • GCN20 SGDID:S0001905, Chr VI from 162482-164740, Verified ORF [yeastflysequest.fasta]
  • Gcn20p [Saccharomyces cerevisiae]gi|51830311|gb|AAU09721.1| YFR009W [Saccharomyces cerevisiae]gi|1169871|sp|P43535|GC20_YEAST GCN20 proteingi|1363661|pir||S56146 GCN20 protein - yeast (Saccharomyces cerevisiae)gi|643479|gb|AAA75444.1| Gcn20pgi|836764 [nrdb_11152004_Chlamydomonas]
  • GCN20 SGDID:S000001905, Chr VI from 162482-164740, Verified ORF, "Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • YFR009W [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA; Gcn20p [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Gcn20p [nr-271106-contam.fasta]
  • Protein GCN20 [nr-271106-contam.fasta]
  • [chemostatdb.fasta]
  • Protein GCN20 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GCN20 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • GCN20 SGDID:S000001905, Chr VI from 162488-164746, Genome Release 64-1-1, Verified ORF, "Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA" [yeast-030211-contam.fasta]
  • GCN20 SGDID:S000001905, Chr VI from 162488-164746, Genome Release 64-2-1, Verified ORF, "Positive regulator of the Gcn2p kinase activity; forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA" [20141208_orf_trans.fasta]
  • YFR009W SGDID:S000001905, Chr VI from 162488-164746, Genome Release 64-1-1, Verified ORF, "Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 752 amino acids

    Gene Ontology:
    Cellular Component: cytosol [IPILink to PubMed article]
    Biological Process: regulation of translational elongation [IMPLink to PubMed article][IPILink to PubMed article]
    Molecular Function: ATPase activity [ISSLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MASIGSQVRK AASSIDPIVT DYAVGYFNHL SGITFDAVQS KQVDLSTEVQ FVSDLLIDAG  60
   61 ASKAKVKELS ESILKQLTTQ LKENEAKLEL TGDTSKRLLD INVLKSHNSK SDINVSLSML 120
  121 GVNGDIEHTG RKMETRVDLK KLAKAEQKIA KKVAKRNNKF VKYEASKLIN DQKEEDYDSF 180
  181 FLQINPLEFG SSAGKSKDIH IDTFDLYVGD GQRILSNAQL TLSFGHRYGL VGQNGIGKST 240
  241 LLRALSRREL NVPKHVSILH VEQELRGDDT KALQSVLDAD VWRKQLLSEE AKINERLKEM 300
  301 DVLRQEFEED SLEVKKLDNE REDLDNHLIQ ISDKLVDMES DKAEARAASI LYGLGFSTEA 360
  361 QQQPTNSFSG GWRMRLSLAR ALFCQPDLLL LDEPSNMLDV PSIAYLAEYL KTYPNTVLTV 420
  421 SHDRAFLNEV ATDIIYQHNE RLDYYRGQDF DTFYTTKEER RKNAQREYDN QMVYRKHLQE 480
  481 FIDKYRYNAA KSQEAQSRIK KLEKLPVLEP PEQDKTIDFK FPECDKLSPP IIQLQDVSFG 540
  541 YDENNLLLKD VNLDVQMDSR IALVGANGCG KTTLLKIMME QLRPLKGFVS RNPRLRIGYF 600
  601 TQHHVDSMDL TTSAVDWMSK SFPGKTDEEY RRHLGSFGIT GTLGLQKMQL LSGGQKSRVA 660
  661 FAALCLNNPH ILVLDEPSNH LDTTGLDALV EALKNFNGGV LMVSHDISVI DSVCKEIWVS 720
  721 EQGTVKRFEG TIYDYRDYIL QSADAAGVVK KH

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) ATP2 CCT6 CLU1 EFT2, EFT1 GCN20 ILV1 MAE1 OLA1 SAM1 TUB3 YHR020W
    View Details Krogan NJ, et al. (2006) GCN1 GCN20 RSA4 UTP14 YHL039W
    View Details Gavin AC, et al. (2006) GCN1 GCN20
    View Details Gavin AC, et al. (2002) ACC1 ADH1 ARO1 ASC1 CCT4 CCT8 CLU1 CPA2 CSE1 DHH1 DUG1 ECM29 FDH1 FKS1 GCN1 GCN20 GFA1 GUS1 HSC82 IDH2 ILV1 KAP123 KTR3 LYS12 MET18 MIR1 MKT1 MRPL3 PFK1 PSA1 RAD3 RAD51 RAD52 RFA1 RGR1 RPA135 RPB2 RPN10 RPN11 RPN12 RPN8 RPN9 RPT3 RPT5 RPT6 RVB2 SAM1 SEC27 SEC7 SHM2 SLC1 SMC3 SRV2 TCB1 TFP1 TIF1, TIF2 TSA1 TUB3 URA7 VAS1 YDJ1 YHB1 YHR020W YOL098C

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run RLF2 identification data - first search in cascade Green EM, et al (2005)
    View Run ZDS1 Sample 2- cpn2 from june 2005 McCusker D, et al (2007)
    View Run ZDS1 Sample 3 - cpn + atp, from june 2005 McCusker D, et al (2007)
    View Run MLP2 #26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2 Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen CTF3 GCN20 1 Unpublished Fields Lab Data
    View Screen HMG1 GCN20 1 Unpublished Fields Lab Data
    View Screen HMG1 GCN20 1 Unpublished Fields Lab Data

    Microscopy / Localization Data

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      PROTEIN(S) PUBLICATION
    View Data GCN20 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..182] deduced N/A No confident structure predictions are available.
    2 View Details [183..491] PSI-BLAST 124.30103 ATP-binding subunit of the histidine permease
    3 View Details [492..752] PSI-BLAST 127.917458 MsbA


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.98

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle