Protein Overview: YCK3

General Information:
Name(s) found:	YCK3 / YER123W [SGD]
Description(s) found: Found 25 descriptions. SHOW ALL	Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway [SGD]
Organism:	Saccharomyces cerevisiae
Length:	524 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] fungal-type vacuole membrane [IDA] plasma membrane [IDA]
Biological Process:	protein amino acid phosphorylation [ISS]
Molecular Function:	casein kinase I activity [ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSQRSSQHIV GIHYAVGPKI GEGSFGVIFE GENILHSCQA QTGSKRDSSI IMANEPVAIK  60
   61 FEPRHSDAPQ LRDEFRAYRI LNGCVGIPHA YYFGQEGMHN ILIIDLLGPS LEDLFEWCGR 120
  121 KFSVKTTCMV AKQMIDRVRA IHDHDLIYRD IKPDNFLISQ YQRISPEGKV IKSCASSSNN 180
  181 DPNLIYMVDF GMAKQYRDPR TKQHIPYRER KSLSGTARYM SINTHFGREQ SRRDDLESLG 240
  241 HVFFYFLRGS LPWQGLKAPN NKLKYEKIGM TKQKLNPDDL LLNNAIPYQF ATYLKYARSL 300
  301 KFDEDPDYDY LISLMDDALR LNDLKDDGHY DWMDLNGGKG WNIKINRRAN LHGYGNPNPR 360
  361 VNGNTARNNV NTNSKTRNTT PVATPKQQAQ NSYNKDNSKS RISSNPQSFT KQQHVLKKIE 420
  421 PNSKYIPETH SNLQRPIKSQ SQTYDSISHT QNSPFVPYSS SKANPKRSNN EHNLPNHYTN 480
  481 LANKNINYQS QRNYEQENDA YSDDENDTFC SKIYKYCCCC FCCC

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		HRR25 YCK1 YCK2 YCK3
View Details	Ho Y, et al. (2002)		BCY1 CAR2 CYS4 GND1 GTO1 LAA1 LYS1 SAH1 SNO2 SSN8 THI22 THO2 TIF1, TIF2 TPK2 TPK3 VPS21 YBR028C YCK1 YCK2 YCK3 YGR111W YPT53
View Details	Qiu et al. (2008)		YCK2 YCK3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	VAM3	Sample: HX11 - both fusion and trans-SNARE complex formation take place.	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control).	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX13 - trans-SNARE complex forms but fusion is blocked.	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX14 - mixture in detergent (control).	Hao Xu, et al. (2010)
View Run	VAM3	hx21: both fusion and trans-SNARE complex formation take place. Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex	Hao Xu, et al. (2010)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	ARB1	YCK3	1	Unpublished Fields Lab Data
View Screen	UBC6	YCK3	1	Unpublished Fields Lab Data
View Screen	ELM1	YCK3	1	Unpublished Fields Lab Data
View Screen	FAR10	YCK3	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	YCK3	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..73] [90..105]	PSI-BLAST	1185.045757	Casein kinase-1, CK1
2	View Details	[74..89] [106..111] [136..204]	PSI-BLAST	1185.045757	Casein kinase-1, CK1
3	View Details	[112..135] [205..392]	PSI-BLAST	1185.045757	Casein kinase-1, CK1
4	View Details	[393..524]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
phosphotransferase activity, alcohol group as acceptor	5.49988294224815	bayes_pls_golite062009
protein kinase activity	5.4586557943719	bayes_pls_golite062009
kinase activity	5.3287080791198	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	4.96052051118534	bayes_pls_golite062009
protein serine/threonine kinase activity	4.46381887831481	bayes_pls_golite062009
transferase activity	3.97138602499442	bayes_pls_golite062009
protein tyrosine kinase activity	2.8153155526437	bayes_pls_golite062009
binding	2.74863538127007	bayes_pls_golite062009
transmembrane receptor activity	2.59526003830791	bayes_pls_golite062009
transmembrane receptor protein kinase activity	2.54265774272269	bayes_pls_golite062009
molecular transducer activity	2.40586892801446	bayes_pls_golite062009
signal transducer activity	2.40586892801446	bayes_pls_golite062009
receptor activity	2.39311927684508	bayes_pls_golite062009
transmembrane receptor protein tyrosine kinase activity	2.16831387024372	bayes_pls_golite062009
receptor signaling protein activity	2.10379226653669	bayes_pls_golite062009
ATP binding	1.97600611746091	bayes_pls_golite062009
adenyl ribonucleotide binding	1.93596671856664	bayes_pls_golite062009
adenyl nucleotide binding	1.90928021591891	bayes_pls_golite062009
receptor signaling protein serine/threonine kinase activity	1.81249386965144	bayes_pls_golite062009
purine ribonucleotide binding	1.65732943334212	bayes_pls_golite062009
ribonucleotide binding	1.65726906484847	bayes_pls_golite062009
purine nucleotide binding	1.63763394711025	bayes_pls_golite062009
nucleotide binding	1.63195828048121	bayes_pls_golite062009
phosphoinositide 3-kinase binding	1.47162554695064	bayes_pls_golite062009
peptidase activity	1.4194137915074	bayes_pls_golite062009
transcription regulator activity	1.30988141770642	bayes_pls_golite062009
protein binding	1.22565748038754	bayes_pls_golite062009
catalytic activity	1.21655087018101	bayes_pls_golite062009
DNA binding	1.17130513163489	bayes_pls_golite062009
nucleic acid binding	1.16049201286103	bayes_pls_golite062009
cytoskeletal protein binding	1.1208666000428	bayes_pls_golite062009
transporter activity	1.11833481501707	bayes_pls_golite062009
peptidase activity, acting on L-amino acid peptides	1.02752755983942	bayes_pls_golite062009
transmembrane transporter activity	0.97575992765505	bayes_pls_golite062009
hydrolase activity	0.950393847838778	bayes_pls_golite062009
transcription factor activity	0.919430701010326	bayes_pls_golite062009
growth factor binding	0.86076618871384	bayes_pls_golite062009
cyclin-dependent protein kinase activity	0.499492353295369	bayes_pls_golite062009
tubulin binding	0.488928950975855	bayes_pls_golite062009
protein serine/threonine/tyrosine kinase activity	0.48345772948903	bayes_pls_golite062009
actin binding	0.464111806616899	bayes_pls_golite062009
substrate-specific transporter activity	0.451090007290896	bayes_pls_golite062009
magnesium ion binding	0.393328812780268	bayes_pls_golite062009
MAP kinase kinase kinase activity	0.37115026509016	bayes_pls_golite062009
telethonin binding	0.366817170817532	bayes_pls_golite062009
MAP kinase kinase activity	0.35402045013148	bayes_pls_golite062009
kinase binding	0.26752427107238	bayes_pls_golite062009
MAP kinase activity	0.26024936404169	bayes_pls_golite062009
protein complex binding	0.239339759110451	bayes_pls_golite062009
transmembrane receptor protein serine/threonine kinase activity	0.20643152368029	bayes_pls_golite062009
transforming growth factor beta receptor activity	0.20643152368029	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	0.205652638854091	bayes_pls_golite062009
receptor binding	0.197402253061255	bayes_pls_golite062009
microtubule binding	0.196718452862042	bayes_pls_golite062009
protein kinase C activity	0.0795689006739899	bayes_pls_golite062009
protein kinase binding	0.00674998152095019	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.0040394359628233	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2