Protein Overview: SPP381

General Information:
Name(s) found:	SPP381 / YBR152W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6319628\|ref\|NP_009710.1\| mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p; Spp381p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S46023 hypothetical protein YBR152w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|586295\|sp\|P38282.1\|SP381_YEAST RecName: Full=Pre-mRNA-splicing factor SPP381; AltName: Full=Suppressor of PRP38-1 mutation [NCBI NR] gi\|536480\|emb\|CAA85111.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|51013559\|gb\|AAT93073.1\| YBR152W [Saccharomyces cerevisiae] [NCBI NR] Uncharacterized protein YBR152W - Saccharomyces cerevisiae (Baker's yeast) [Swiss-Prot] Pre-mRNA-splicing factor SPP381 OS=Saccharomyces cerevisiae GN=SPP381 PE=1 SV=1 [Swiss-Prot] SPP381 Suppressor of Pre-mRNA Processing mutant [mips] mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p [SGD] SPP381, Chr II from 546334-547209 [yeast_orfs2] Chr II from 546334-547209 [yeastorf3.fasta] Chr II from 546333-547208 [yeastorf4.fasta] Chr II from 546331-547206 [yeastorf5.fasta] (P38282) Hypothetical 33.8 kDa protein in ARA1-RIB7 intergenic region [4932.FASTACCERV.txt] SPP381 SGDID:S0000356, Chr II from 546332-547207, Verified ORF [yeastflysequest.fasta] SPP381 SGDID:S0000356, Chr II from 546370-547245, Verified ORF [10112005cervSGD.fasta] Spp381p [Saccharomyces cerevisiae]gi\|51013559\|gb\|AAT93073.1\| YBR152W [Saccharomyces cerevisiae]gi\|536480\|emb\|CAA85111.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|626526\|pir\|\|S46023 hypothetical protein YBR152w - yeast (Saccharomyces cerevis [nrdb_11152004_Chlamydomonas] SPP381 SGDID:S000000356, Chr II from 546370-547245, Verified ORF, "mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Uncharacterized protein YBR152W [nr-271106-contam.fasta] mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p; Spp381p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YBR152W [Saccharomyces cerevisiae] [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Pre-mRNA-splicing factor SPP381 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SPP381 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SPP381 SGDID:S000000356, Chr II from 546376-547251, Genome Release 64-1-1, Verified ORF, "mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p" [yeast-030211-contam.fasta] SPP381 SGDID:S000000356, Chr II from 546376-547251, Genome Release 64-2-1, Verified ORF, "mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p; relocalizes to the cytosol in response to hypoxia" [20141208_orf_trans.fasta] TR1096\|c0_g1_i1\|g.856 ORF TR1096\|c0_g1_i1\|g.856 TR1096\|c0_g1_i1\|m.856 type:complete len:292 (+) TR1096\|c0_g1_i1:190-1065(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YBR152W SGDID:S000000356, Chr II from 546376-547251, Genome Release 64-1-1, Verified ORF, "mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_4734\|g.3480 ORF asmbl_4734\|g.3480 asmbl_4734\|m.3480 type:complete len:292 (+) asmbl_4734:190-1065(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_4739\|g.3012 ORF ID=asmbl_4739\|g.3012 ID=asmbl_4739\|m.3012 type:complete len:292 (+) ID=asmbl_4739:190-1065(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	291 amino acids
Gene Ontology:
Cellular Component:	U4/U6 x U5 tri-snRNP complex [IDA ]
Biological Process:	generation of catalytic spliceosome for first transesterification step [IGI] nuclear mRNA splicing, via spliceosome [IMP ]
Molecular Function:	protein binding [IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSFRHFKRRL DTSSADESSS ADEEHPDQNV SLTEKSASLS HSDLGGEILN GTGKNRTPND  60
   61 GQESNESDGS PESDESPESE ESSDNSDSSD SDDMRPLPRP LFMKKKANNL QKATKIDQPW 120
  121 NAQDDARVLQ TKKENMIKNI DKANQVAKNY ETMKLRLNTN YSTNEELIKQ CLLLDDNDEV 180
  181 DSEKERQKWF ERQNERKQKH RRIQLAKQRE SEEYEAKRFE AMQKGKDGNT KYDVILDKEK 240
  241 EKLDHKKQRS AEKVEKSHNN NRYKITRTKN VEFGDLGKNS RDYEETEYSV I

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		PRP3 PRP31 PRP38 PRP4 PRP6 SNU23 SNU66 SPP381
View Details	(MIPS) Mewes HW, et al. (2004)		AAR2 BRR1 BRR2 CBC2 CDC40 CWC15 CWC2 CWC22 CWC23 DHR2 LSM4 MAK5 MUD2 PRP16 PRP18 PRP2 PRP22 PRP24 PRP28 PRP31 PRP38 PRP39 PRP4 PRP40 PRP43 PRP5 PRP6 PRP8 SLU7 SMD1 SMD3 SME1 SNP1 SPP2 SPP381 STO1 YHC1
View Details	Krogan NJ, et al. (2006)		PRP38 SNU23 SPP381
View Details	Gavin AC, et al. (2006)		BRR2 CUS1 LEA1 PRP21 PRP3 PRP31 PRP4 PRP6 PRP8 PRP9 SMB1 SNU114 SNU23 SNU66 SPP381
View Details	Gavin AC, et al. (2002)		AAR2 BRR1 BUD31 CDC33 CEF1 CLF1 CSN12 CUS1 CWC2 CWC22 CWC23 DHH1 DIB1 ECM2 GCN2 HSH155 HTA1 IMG1 LEA1 LIN1 LSM1 LSM2 LSM4 LSM5 LSM6 LSM7 LUC7 MRP7 MRPL28 MRPL3 MRPL35 MRPL38 MRPL4 MRPL8 MRPS5 MSL1 MUD1 PAT1 PRP11 PRP18 PRP19 PRP21 PRP24 PRP28 PRP3 PRP31 PRP38 PRP39 PRP4 PRP40 PRP42 PRP43 PRP46 PRP6 PRP8 PRP9 RSE1 RVB2 SLU7 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNP1 SNT309 SNU114 SNU23 SNU56 SNU66 SNU71 SPP381 SPP382 SRB2 STO1 SYF1 TOS4 UTP21 YML6
View Details	Qiu et al. (2008)		AAR2 BRR1 BRR2 BUD31 CBC2 CDC40 CEF1 CLF1 CUS1 CUS2 CWC15 CWC2 CWC21 CWC22 CWC23 DHR2 DIB1 ECM2 HSH155 HSH49 IST3 ISY1 LEA1 LSM2 LSM3 LSM4 LSM5 LSM6 LSM7 LSM8 LUC7 MAK5 MSL1 MUD1 MUD2 NAM8 NTC20 PRP11 PRP16 PRP18 PRP19 PRP2 PRP21 PRP22 PRP24 PRP28 PRP3 PRP31 PRP38 PRP39 PRP4 PRP40 PRP42 PRP43 PRP45 PRP46 PRP5 PRP6 PRP8 PRP9 RSE1 SLU7 SMB1 SMD1 SMD2 SMD3 SME1 SMX2 SMX3 SNP1 SNT309 SNU114 SNU23 SNU56 SNU66 SNU71 SPP2 SPP381 SPP382 STO1 SUB2 SYF1 SYF2 YHC1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	None Entered	#17 Mitotic Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	SPP381	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..67]	ORFEUS	7.29	No description for 1l8mA was found.
2	View Details	[68..146]	ORFEUS	7.29	No description for 1l8mA was found.
3	View Details	[147..216]	ORFEUS	7.29	No description for 1l8mA was found.
4	View Details	[217..291]	ORFEUS	7.29	No description for 1l8mA was found.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Protein Overview: SPP381

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: