Protein Overview: HEK2

General Information:
Name(s) found:	HEK2 / YBL032W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6319439\|ref\|NP_009521.1\| RNA binding protein with similarity to hnRNP-K that localizes to the cytoplasm and to subtelomeric DNA; required for the proper localization of ASH1 mRNA; involved in the regulation of telomere position effect and telomere leng [NCBI NR] pir\|\|S45766 hypothetical protein YBL032w - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|602889\|emb\|CAA54496.1\| YBL0418 [Saccharomyces cerevisiae] [NCBI NR] gi\|586417\|sp\|P38199.1\|YBD2_YEAST RecName: Full=KH domain-containing protein YBL032W [NCBI NR] gi\|536039\|emb\|CAA84852.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] KH domain-containing protein YBL032W OS=Saccharomyces cerevisiae GN=YBL032W PE=1 SV=1 [Swiss-Prot] HEK2 Heterogeneous nuclear RNP K-like gene [mips] RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K [SGD] YBL032W, Chr II from 160146-161291 [yeast_orfs2] Chr II from 160146-161291 [yeastorf3.fasta] (P38199) Hypothetical 41.7 kDa protein in RIB1-AAC2 intergenic region [4932.FASTACCERV.txt] HEK2 SGDID:S0000128, Chr II from 160149-161294, Verified ORF [yeastflysequest.fasta] HEK2 SGDID:S0000128, Chr II from 160187-161332, Verified ORF [10112005cervSGD.fasta] Hek2p [Saccharomyces cerevisiae]gi\|602889\|emb\|CAA54496.1\| YBL0418 [Saccharomyces cerevisiae]gi\|536039\|emb\|CAA84852.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|626477\|pir\|\|S45766 hypothetical protein YBL032w - yeast (Saccharomyces cerevisiae [nrdb_11152004_Chlamydomonas] HEK2 SGDID:S000000128, Chr II from 160187-161332, Verified ORF, "RNA binding protein with similarity to hnRNP-K that localizes to the cytoplasm and to subtelomeric DNA; required for the proper localization of ASH1 mRNA; involved in the regulation of telom [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] HEK2 SGDID:S000000128, Chr II from 160187-161332, Verified ORF, "RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere positio [yeast-200209-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YBL0418 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] KH domain-containing protein YBL032W [nr-271106-contam.fasta] RNA binding protein with similarity to hnRNP-K that localizes to the cytoplasm and to subtelomeric DNA; required for the proper localization of ASH1 mRNA; involved in the regulation of telomere position effect and telomere length; Hek2p [Saccharomyces cer [nr-271106-contam.fasta] HEK2 SGDID:S000000128, Chr II from 160187-161332, Verified ORF, "RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K" [yeast-bovrabprots.fasta] HEK2 SGDID:S000000128, Chr II from 160187-161332, Verified ORF, "RNA binding protein with similarity to hnRNP-K that localizes to the cytoplasm and to subtelomeric DNA; required for the proper localization of ASH1 mRNA; involved in the regulation of telomere position effect and telomere length" [yeast-contam_custom.fasta] [chemostatdb.fasta] HEK2 SGDID:S000000128, Chr II from 160184-161329, Genome Release 64-1-1, Verified ORF, "RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K" [yeast-030211-contam.fasta] HEK2 SGDID:S000000128, Chr II from 160184-161329, Genome Release 64-2-1, Verified ORF, "RNA binding protein involved in asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K" [20141208_orf_trans.fasta] TR5704\|c0_g1_i1\|g.3921 ORF TR5704\|c0_g1_i1\|g.3921 TR5704\|c0_g1_i1\|m.3921 type:complete len:382 (+) TR5704\|c0_g1_i1:130-1275(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YBL032W SGDID:S000000128, Chr II from 160184-161329, Genome Release 64-1-1, Verified ORF, "RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] ID=asmbl_4581\|g.2826 ORF ID=asmbl_4581\|g.2826 ID=asmbl_4581\|m.2826 type:complete len:382 (+) ID=asmbl_4581:182-1327(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	381 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA] nuclear chromosome, telomeric region [IDA]
Biological Process:	intracellular mRNA localization [IMP][IPI] telomere maintenance via telomerase [IMP][IPI]
Molecular Function:	mRNA binding [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSQFFEAATP VAIPTNNTNG GSSDAGSAAT GGAPVVGTTA QPTINHRLLL SLKEAAKIIG  60
   61 TKGSTISRIR AANAVKIGIS EKVPGCSDRI LSCAGNVINV ANAIGDIVDV LNKRNPENED 120
  121 AAEGEAEEHY YFHFLNHILP APSKDEIRDL QQLEDIGYVR LIVANSHISS IIGKAGATIK 180
  181 SLINKHGVKI VASKDFLPAS DERIIEIQGF PGSITNVLIE ISEIILSDVD VRFSTERSYF 240
  241 PHLKKSSGEP TSPSTSSNTR IELKIPELYV GAIIGRGMNR IKNLKTFTKT NIVVERKDDD 300
  301 DKDENFRKFI ITSKFPKNVK LAESMLLKNL NTEIEKRENY KRKLEAAEGD ATVVTERSDS 360
  361 ASFLEEKEEP QENHDNKEEQ S

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		ARC1 ATG14 CAT8 CCC2 HEK2 IRC20 MCA1 MES1 MGA2 NCS2 NHA1 PET127 PHO89 PHO91 YKL050C YLR363W-A
View Details	Gavin AC, et al. (2002)		ASC1 CBF5 CDC33 DBP2 HEK2 HHF1, HHF2 LHP1 MIS1 MRP7 NAB6 POL2 PUS7 RBG1 ROM2 SBP1 SNP1 SRO9 STM1 STO1 SUI3 TIF1, TIF2 TIF4631 TIF4632 UTP20 YMR310C
View Details	Qiu et al. (2008)		HEK2 RAP1 RIF1 SUB2

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	YPI1	HEK2	2	Hazbun TR, et al. (2003)
View Screen	YLR224W	HEK2	1	Unpublished Fields Lab Data
View Screen	SPC98	HEK2	1	Unpublished Fields Lab Data
View Screen	HEK2	TUP1	1	Unpublished Fields Lab Data
View Screen	HEK2	RTS3	1	Unpublished Fields Lab Data
View Screen	HEK2	ARG2	1	Unpublished Fields Lab Data
View Screen	HEK2	PIK1	1	Unpublished Fields Lab Data
View Screen	HEK2	HST1	1	Unpublished Fields Lab Data
View Screen	HEK2	TYE7	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	HEK2	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..42] [165..238]	PSI-BLAST	36.30103	Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domain 3; S1 RNA-binding domain of polyribonucleotide phosphorylase, PNPase; Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domains 1 and 4; Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domain 6; Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domains 2 and 5
2	View Details	[43..164] [239..381]	PSI-BLAST	36.30103	Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domain 3; S1 RNA-binding domain of polyribonucleotide phosphorylase, PNPase; Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domains 1 and 4; Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domain 6; Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domains 2 and 5

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
transcription regulator activity	3.9432182987214	bayes_pls_golite062009
structural constituent of ribosome	3.63349555627235	bayes_pls_golite062009
structural molecule activity	3.43604616110432	bayes_pls_golite062009
nucleic acid binding	2.14875774646095	bayes_pls_golite062009
binding	1.9759530321894	bayes_pls_golite062009
nucleotidyltransferase activity	1.69410216708845	bayes_pls_golite062009
DNA binding	1.688021219058	bayes_pls_golite062009
deacetylase activity	1.09789613642093	bayes_pls_golite062009
exonuclease activity	1.02947145936572	bayes_pls_golite062009
transcription termination factor activity	1.01912880224809	bayes_pls_golite062009
exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	0.96549462572212	bayes_pls_golite062009
ribonuclease activity	0.75307991342421	bayes_pls_golite062009
protein binding	0.523813050066193	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.499351238958464	bayes_pls_golite062009
3'-5'-exoribonuclease activity	0.48105441429954	bayes_pls_golite062009
3'-5' exonuclease activity	0.477960254167	bayes_pls_golite062009
exoribonuclease activity, producing 5'-phosphomonoesters	0.36703663415044	bayes_pls_golite062009
exoribonuclease activity	0.350394896843651	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	0.337324481645901	bayes_pls_golite062009
DNA-(apurinic or apyrimidinic site) lyase activity	0.288026663667102	bayes_pls_golite062009
RNA binding	0.24714225183821	bayes_pls_golite062009
kinase activity	0.237670019875888	bayes_pls_golite062009
damaged DNA binding	0.16864489654021	bayes_pls_golite062009
catalytic activity	0.10089766806364	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.0869020377778909	bayes_pls_golite062009
pyrophosphatase activity	0.0193674670513169	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2