Protein Overview: PMS1

General Information:
Name(s) found:	PMS1 / YNL082W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|86161606\|gb\|ABC86936.1\| PMS1 [Saccharomyces cerevisiae] [NCBI NR] gi\|86161604\|gb\|ABC86935.1\| PMS1 [Saccharomyces cerevisiae] [NCBI NR] gi\|86161602\|gb\|ABC86934.1\| PMS1 [Saccharomyces cerevisiae] [NCBI NR] gi\|86161598\|gb\|ABC86932.1\| PMS1 [Saccharomyces cerevisiae] [NCBI NR] gi\|46562124\|ref\|NP_014317.2\| ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL; Pms1p [Saccharomyces cer [NCBI NR] gi\|110282983\|sp\|P14242.3\|PMS1_YEAST RecName: Full=DNA mismatch repair protein PMS1; AltName: Full=Postmeiotic segregation protein 1 [NCBI NR] DNA mismatch repair protein PMS1 OS=Saccharomyces cerevisiae GN=PMS1 PE=1 SV=3 [Swiss-Prot] PMS1 DNA mismatch repair protein [mips] ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL [SGD] PMS1 SGDID:S0005026, Chr XIV from 473390-476011, Verified ORF [yeastflysequest.fasta] Pms1p [Saccharomyces cerevisiae] [nrdb_11152004_Chlamydomonas] PMS1 SGDID:S000005026, Chr XIV from 473392-476013, Verified ORF, "ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] PMS1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] DNA mismatch repair protein PMS1 (Postmeiotic segregation protein 1) [nr-271106-contam.fasta] ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL; Pms1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] PMS1 SGDID:S000005026, Chr XIV from 473393-476014, Verified ORF, "ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E [SGD_S-cerevisiae_na_01-05-2010_reversed.fasta] PMS1 SGDID:S000005026, Chr XIV from 473392-476013, Verified ORF, "ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL" [yeast-bovrabprots.fasta] [chemostatdb.fasta] PMS1 SGDID:S000005026, Chr XIV from 473393-476014, Verified ORF, "ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL" [SGDyeast.20101221.fasta] DNA mismatch repair protein PMS1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PMS1 PE=1 SV=3 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] PMS1 SGDID:S000005026, Chr XIV from 473391-476012, Genome Release 64-1-1, Verified ORF, "ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL" [yeast-030211-contam.fasta] PMS1 SGDID:S000005026, Chr XIV from 473391-476012, Genome Release 64-2-1, Verified ORF, "ATP-binding protein required for mismatch repair; required for both mitosis and meiosis; functions as a heterodimer with Mlh1p; binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL" [20141208_orf_trans.fasta] YNL082W SGDID:S000005026, Chr XIV from 473391-476012, Genome Release 64-1-1, Verified ORF, "ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	873 amino acids
Gene Ontology:
Cellular Component:	nuclear chromosome [TAS]
Biological Process:	meiotic mismatch repair [IMP] mismatch repair [TAS] meiosis [TAS]
Molecular Function:	ATPase activity [IDA] DNA binding [IDA] ATP binding [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTQIHQINDI DVHRITSGQV ITDLTTAVKE LVDNSIDANA NQIEIIFKDY GLESIECSDN  60
   61 GDGIDPSNYE FLALKHYTSK IAKFQDVAKV QTLGFRGEAL SSLCGIAKLS VITTTSPPKA 120
  121 DKLEYDMVGH ITSKTTTSRN KGTTVLVSQL FHNLPVRQKE FSKTFKRQFT KCLTVIQGYA 180
  181 IINAAIKFSV WNITPKGKKN LILSTMRNSS MRKNISSVFG AGGMRGLEEV DLVLDLNPFK 240
  241 NRMLGKYTDD PDFLDLDYKI RVKGYISQNS FGCGRNSKDR QFIYVNKRPV EYSTLLKCCN 300
  301 EVYKTFNNVQ FPAVFLNLEL PMSLIDVNVT PDKRVILLHN ERAVIDIFKT TLSDYYNRQE 360
  361 LALPKRMCSQ SEQQAQKRLK TEVFDDRSTT HESDNENYHT ARSESNQSNH AHFNSTTGVI 420
  421 DKSNGTELTS VMDGNYTNVT DVIGSECEVS VDSSVVLDEG NSSTPTKKLP SIKTDSQNLS 480
  481 DLNLNNFSNP EFQNITSPDK ARSLEKVVEE PVYFDIDGEK FQEKAVLSQA DGLVFVDNEC 540
  541 HEHTNDCCHQ ERRGSTDTEQ DDEADSIYAE IEPVEINVRT PLKNSRKSIS KDNYRSLSDG 600
  601 LTHRKFEDEI LEYNLSTKNF KEISKNGKQM SSIISKRKSE AQENIIKNKD ELEDFEQGEK 660
  661 YLTLTVSKND FKKMEVVGQF NLGFIIVTRK VDNKYDLFIV DQHASDEKYN FETLQAVTVF 720
  721 KSQKLIIPQP VELSVIDELV VLDNLPVFEK NGFKLKIDEE EEFGSRVKLL SLPTSKQTLF 780
  781 DLGDFNELIH LIKEDGGLRR DNIRCSKIRS MFAMRACRSS IMIGKPLNKK TMTRVVHNLS 840
  841 ELDKPWNCPH GRPTMRHLME LRDWSSFSKD YEI

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		MLH1 PMS1
View Details	(MIPS) Mewes HW, et al. (2004)		MSH2 MSH3 PMS1
View Details	Krogan NJ, et al. (2006)		NSA1 PMS1 RDR1
View Details	Gavin AC, et al. (2006)		MLH1 PMS1
View Details	Gavin AC, et al. (2002)		CMR1 DNA2 HDA1 HDA2 HDA3 HSC82 KAP95 LCD1 MEC1 MGM101 MLH1 MPH1 MSH2 MSH3 MSH6 OLA1 PGK1 PMS1 PRO2 RAD16 RAD52 RFA1 RFA2 RFA3 RIM1 RNH1 RSC8 RVB2 SGS1 SHS1 TIF1, TIF2 TOP3 VAS1 VPS1 YHR122W
View Details	Qiu et al. (2008)		MSH2 PMS1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	ATG12	PMS1	1	Unpublished Fields Lab Data
View Screen	UBI4	PMS1	1	Unpublished Fields Lab Data
View Screen	UBC7	PMS1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	PMS1	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..403]	PSI-BLAST	68.39794	DNA mismatch repair protein PMS2
2	View Details	[404..590]	MSA	4.434992	View MSA. No confident structure predictions are available.
3	View Details	[591..733]	MSA	1.139986	View MSA. No confident structure predictions are available.
4	View Details	[734..826]	deduced	N/A	No confident structure predictions are available.
5	View Details	[827..873]	MSA	3.062993	View MSA. No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
mismatched DNA binding	8.69038541722775	bayes_pls_golite062009
DNA insertion or deletion binding	7.03361327424191	bayes_pls_golite062009
	6.04882684441736	bayes_pls_golite062009
guanine/thymine mispair binding	5.8954303695758	bayes_pls_golite062009
mismatch repair complex binding	5.63566878749852	bayes_pls_golite062009
single base insertion or deletion binding	5.36719395837475	bayes_pls_golite062009
dinucleotide insertion or deletion binding	4.91072939712457	bayes_pls_golite062009
loop DNA binding	4.26052361441101	bayes_pls_golite062009
DNA topoisomerase (ATP-hydrolyzing) activity	2.66323092512867	bayes_pls_golite062009
DNA topoisomerase activity	2.35776058207527	bayes_pls_golite062009
protein kinase activity	2.13536672517084	bayes_pls_golite062009
catalytic activity	2.10890475146569	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	1.77725015786603	bayes_pls_golite062009
kinase activity	1.68443268281997	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	1.64025200399404	bayes_pls_golite062009
transcription regulator activity	1.37978051130887	bayes_pls_golite062009
DNA binding	1.31521246060081	bayes_pls_golite062009
structure-specific DNA binding	1.22640171545155	bayes_pls_golite062009
nucleic acid binding	1.22084067619837	bayes_pls_golite062009
transferase activity	1.09369139087478	bayes_pls_golite062009
exonuclease activity	1.02947145936572	bayes_pls_golite062009
molecular transducer activity	0.996368427522977	bayes_pls_golite062009
signal transducer activity	0.996368427522977	bayes_pls_golite062009
double-stranded DNA binding	0.91402301750067	bayes_pls_golite062009
binding	0.908938377164829	bayes_pls_golite062009
exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	0.86218415868631	bayes_pls_golite062009
MutSalpha complex binding	0.8423412194292	bayes_pls_golite062009
single-stranded DNA binding	0.839521076707668	bayes_pls_golite062009
ribonuclease activity	0.75307991342421	bayes_pls_golite062009
structural constituent of ribosome	0.71251019785001	bayes_pls_golite062009
nuclease activity	0.69721312877891	bayes_pls_golite062009
3'-5'-exoribonuclease activity	0.48105441429954	bayes_pls_golite062009
3'-5' exonuclease activity	0.477960254167	bayes_pls_golite062009
structural molecule activity	0.41673693977319	bayes_pls_golite062009
exoribonuclease activity, producing 5'-phosphomonoesters	0.36703663415044	bayes_pls_golite062009
exoribonuclease activity	0.350394896843651	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.97

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2