Protein Overview: CIK1

General Information:
Name(s) found:	CIK1 / YMR198W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|642291\|emb\|CAA87820.1\| Cik1p [Saccharomyces cerevisiae] [NCBI NR] gi\|6323854\|ref\|NP_013925.1\| Kinesin-associated protein required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; has similarity to Vik1p; [NCBI NR] gi\|399252\|sp\|Q01649.1\|CIK1_YEAST RecName: Full=Spindle pole body-associated protein CIK1; AltName: Full=Chromosome instability and karyogamy protein 1 [NCBI NR] pir\|\|A44073 CIK1 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|171222\|gb\|AAA34494.1\| spindle pole body associated protein [NCBI NR] Spindle pole body-associated protein CIK1 OS=Saccharomyces cerevisiae GN=CIK1 PE=1 SV=1 [Swiss-Prot] CIK1 Spindle pole body associated protein [mips] Kinesin-associated protein required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; has similarity to Vik1p [SGD] CIK1, Chr XIII from 659744-661528 [yeast_orfs2] Chr XIII from 659744-661528 [yeastorf3.fasta] (Q01649) Spindle pole body associated protein [4932.FASTACCERV.txt] CIK1 SGDID:S0004811, Chr XIII from 659744-661528, Verified ORF [yeastflysequest.fasta] CIK1 is important for proper organiziation of microtubule arrays and establishment of a spindle; is essential for karyogamy; and expression is regulated by KAR4 and mating; spindle pole body associated protein [Saccharomyces cerevisiae]gi\|642291\|emb\|CAA8 [nrdb_11152004_Chlamydomonas] CIK1 SGDID:S000004811, Chr XIII from 659744-661528, Verified ORF, "Kinesin-associated protein required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Spindle pole body-associated protein [nr-271106-contam.fasta] spindle pole body associated protein [nr-271106-contam.fasta] Kinesin-associated protein required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; has similarity to Vik1p; Cik1p [Saccharomyces cerevi [nr-271106-contam.fasta] Cik1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] CIK1 SGDID:S000004811, Chr XIII from 659744-661528, Verified ORF, "Kinesin-associated protein required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; has similarity to Vik1p" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Spindle pole body-associated protein CIK1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CIK1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] CIK1 SGDID:S000004811, Chr XIII from 659745-661529, Genome Release 64-1-1, Verified ORF, "Kinesin-associated protein required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; has similarity to Vik1p" [yeast-030211-contam.fasta] CIK1 SGDID:S000004811, Chr XIII from 659745-661529, Genome Release 64-2-1, Verified ORF, "Kinesin-associated protein; required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; locus encodes a long and short transcript with differing functions; CIK1 has a paralog, VIK1, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YMR198W SGDID:S000004811, Chr XIII from 659745-661529, Genome Release 64-1-1, Verified ORF, "Kinesin-associated protein required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; has similarity to Vik1p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	594 amino acids
Gene Ontology:
Cellular Component:	spindle pole body [IDA ] spindle [IDA] kinesin complex [IDA] cytoplasmic microtubule [TAS]
Biological Process:	establishment of mitotic spindle orientation [TAS] mitotic sister chromatid segregation [TAS] mitotic spindle organization in nucleus [TAS] karyogamy involved in conjugation with cellular fusion [IMP] meiosis [IDA][IMP]
Molecular Function:	microtubule motor activity [IMP][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNNSKIPKLS FHSDPNNVTR DFPKTKRQKV QKREMDMILT PNNNKLNILH SSGSGIRRCY  60
   61 TDDTSATYTK KLTFGGDPKI IERVKNNERK VRKDIDSLLN AISEIEKESV RIHARELPAI 120
  121 TLELDAKVKA CRELQNEIDG LSTEMDLKDN QCDLQRKNVE LSSKNIVSMH AVKVQEFEND 180
  181 LEEELSNAKR EWTYKLMEVE NLKPDERLTD EMRQLKTEFE EVNRKLFILQ NENENECKNY 240
  241 KKELDKKFEI FKKVKNDARI ELDGEQERLS KVLKDLQDTH GELKENIKTC RDEFNDFEKR 300
  301 IGEAEVNFHS MELAVVPLKK KLASTSQALT QVQEEKKQVE GEANNWKKKY VNELEKVQQE 360
  361 LYTRQNLATS IEEIKGYTRC FAYANERQMP DEFHINYVDR CICENSGEKR VQVFDRVVLE 420
  421 EIHKDHKRLY NECIPFLEKY ISKLINCSII VVSQQPTAPM KKTLLKQLIE QYGENYKMTL 480
  481 NILHLDGSIK HSDVGLDNPT EIRDLSQDEE CMNILTLDTK LGKDEESHSM NIYIGSMSTV 540
  541 QLNRELDDAP SVLSHILTKT KQCFVFKINA GENIEKALAL AGKLKRTITL PQLD

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CIK1 REV1
View Details	(MIPS) Mewes HW, et al. (2004)		AME1 ARP1 BIK1 CIK1 CIN8 COG7 DYN1 JNM1 KAR3 KAR5 KIP1 KIP2 KIP3 LIN1 NUF2 SPC19 SPC34 STU1 STU2 TID3
View Details	Krogan NJ, et al. (2006)		ARG3 ATP3 BUD30 CIK1 GAL80 PMI40 SGN1 YGR250C YML002W YOR1
View Details	Ho Y, et al. (2002)		CIK1 CLU1
View Details	Qiu et al. (2008)		ARP10 BIK1 BIM1 CIK1 CIN8 DYN1 DYN2 DYN3 HSK3 JNM1 KAR3 KIP1 KIP3 MYO2 NIP100 NUF2 PAC1 PAC11 SFI1 SLK19 SPC19 SPC34 STU1 STU2 VIK1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SCL1	CIK1	1	Unpublished Fields Lab Data
View Screen	MDM30	CIK1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	CIK1	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..362]	PSI-BLAST	13.69897	Heavy meromyosin subfragment
2	View Details	[363..406]	PSI-BLAST	9.30103	Tropomyosin
3	View Details	[407..523]	PSI-BLAST	9.30103	Tropomyosin
4	View Details	[524..594]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2