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Protein Overview: SPC110

General Information:
Name(s) found: SPC110 / YDR356W [SGD]
Description(s) found:
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  • gi|849173|gb|AAB64791.1| Nuf1p: Probable essential component of the nucleoskeleton (Swiss Prot. accession number P32380) [Saccharomyces cerevisiae] [NCBI NR]
  • gi|6320562|ref|NP_010643.1| Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner; [NCBI NR]
  • gi|417392|sp|P32380.1|NUF1_YEAST RecName: Full=Protein NUF1; AltName: Full=Spindle poly body spacer protein SPC110 [NCBI NR]
  • gi|4070|emb|CAA77668.1| nuf1 [Saccharomyces cerevisiae] [NCBI NR]
  • gi|312175|emb|CAA51733.1| SPC110/NUF1 [Saccharomyces cerevisiae] [NCBI NR]
  • pir||S26710 spindle pole body protein NUF1 - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • Protein NUF1 OS=Saccharomyces cerevisiae GN=NUF1 PE=1 SV=1 [Swiss-Prot]
  • SPC110 Spindle pole body component [mips]
  • Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner [SGD]
  • NUF1, Chr IV from 1186096-1188930 [yeast_orfs2]
  • Chr IV from 1186096-1188930 [yeastorf3.fasta]
  • Chr IV from 1186095-1188929 [yeastorf4.fasta]
  • (P32380) NUF1 protein (Spindle poly body spacer protein SPC110) [4932.FASTACCERV.txt]
  • SPC110 SGDID:S0002764, Chr IV from 1186096-1188930, Verified ORF [yeastflysequest.fasta]
  • SPC110 SGDID:S0002764, Chr IV from 1186098-1188932, Verified ORF [10112005cervSGD.fasta]
  • Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner [Saccharomyces cerevisiae]g [nrdb_11152004_Chlamydomonas]
  • SPC110 SGDID:S000002764, Chr IV from 1186098-1188932, Verified ORF, "Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • SPC110 SGDID:S000002764, Chr IV from 1186100-1188934, Verified ORF, "Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated [yeast-contam.fasta]
  • SPC110 SGDID:S000002764, Chr IV from 1186101-1188935, Verified ORF, "Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta]
  • Protein NUF1 (Spindle poly body spacer protein SPC110) [nr-271106-contam.fasta]
  • nuf1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner; Spc110p [Saccharomyces cere [nr-271106-contam.fasta]
  • SPC110/NUF1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Nuf1p: Probable essential component of the nucleoskeleton (Swiss Prot. accession number P32380) [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • SPC110 SGDID:S000002764, Chr IV from 1186101-1188935, Verified ORF, "Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner" [yeast-bovrabprots.fasta]
  • SPC110 SGDID:S000002764, Chr IV from 1186100-1188934, Verified ORF, "Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner" [yeast-contam_custom.fasta]
  • [chemostatdb.fasta]
  • SPC110 SGDID:S000002764, Chr IV from 1186107-1188941, Genome Release 64-1-1, Verified ORF, "Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner" [yeast-030211-contam.fasta]
  • SPC110 SGDID:S000002764, Chr IV from 1186107-1188941, Genome Release 64-2-1, Verified ORF, "Inner plaque spindle pole body (SPB) component; ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner" [20141208_orf_trans.fasta]
  • YDR356W SGDID:S000002764, Chr IV from 1186107-1188941, Genome Release 64-1-1, Verified ORF, "Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 944 amino acids

    Gene Ontology:
    Cellular Component: inner plaque of spindle pole body [IDALink to PubMed article]
    central plaque of spindle pole body [IDALink to PubMed article]
    Biological Process: microtubule nucleation [IPILink to PubMed article]
    Molecular Function: structural constituent of cytoskeleton [IPILink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDEASHLPNG SLKNMEFTPV GFIKSKRNTT QTQVVSPTKV PNANNGDENE GPVKKRQRRS  60
   61 IDDTIDSTRL FSEASQFDDS FPEIKANIPP SPRSGNVDKS RKRNLIDDLK KDVPMSQPLK 120
  121 EQEVREHQMK KERFDRALES KLLGKRHITY ANSDISNKEL YINEIKSLKH EIKELRKEKN 180
  181 DTLNNYDTLE EETDDLKNRL QALEKELDAK NKIVNSRKVD DHSGCIEERE QMERKLAELE 240
  241 RKLKTVKDQV LELENNSDVQ SLKLRSKEDE LKNLMNELNE LKSNAEEKDT QLEFKKNELR 300
  301 KRTNELNELK IKSDEMDLQL KQKQNESKRL KDELNELETK FSENGSQSSA KENELKMLKN 360
  361 KIAELEEEIS TKNSQLIAKE GKLASLMAQL TQLESKLNQR DSQLGSREEE LKKTNDKLQK 420
  421 DIRIAREETV SKDERIIDLQ KKVKQLENDL FVIKKTHSES KTITDNELES KDKLIKILEN 480
  481 DLKVAQEKYS KMEKELKERE FNYKISESKL EDEKTTLNEK ISNLAAENSQ LKNKIEDNST 540
  541 ATHHMKENYE KQLESLRKDI EEYKESAKDS EDKIEELKIR IAENSAKVSE KRSKDIKQKD 600
  601 EQISDLTQNL KLQEDEISSL KSIIDRYKKD FNQLKSEQSN IQHDLNLQIL NLENKLIESE 660
  661 DELKSLRDSQ KIEIENWKRK YNNLSLENDR LLTEKESASD KEREISILNR KLDEMDKEKW 720
  721 NLQESKEKYK RELQKVITAN DRLRREKEEL NENSNNIRIM EDKMTRIKKN YLSEITSLQE 780
  781 ENRRLEERLI LNERRKDNDS TMQLNDIISY YKLKYHSEVR HNNDLKVIND YLNKVLALGT 840
  841 RRLRLDTRKG EHSLNISLPD DDELDRDYYN SHVYTRYHDY EYPLRFNLNR RGPYFERRLS 900
  901 FKTVALLVLA CVRMKRIAFY RRSDDNRLRI LRDRIESSSG RISW

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details (MIPS) Mewes HW, et al. (2004) BBP1 CDC31 CMD1 CNM67 KAR1 NUD1 SPC105 SPC110 SPC24 SPC25 SPC29 SPC42 SPC72
    View Details Gavin AC, et al. (2002) CDC48 GIM3 GIM5 PAC10 PFK1 SPC110 SPC97 SPC98 TUB4 YKE2
    View Details Ho Y, et al. (2002) CMD1 CMP2 HUL5 MLC1 MYO2 MYO3 MYO4 MYO5 SHE4 SPC110
    View Details Qiu et al. (2008) BBP1 BIK1 CDC31 CMD1 CNM67 KAR1 MPS2 NDC1 NUD1 NUF2 SLK19 SPC105 SPC110 SPC19 SPC24 SPC25 SPC29 SPC42 SPC72 SPC97 SPC98 STU1 STU2 TID3 TUB4

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run MLP2 #33 Asynchronous SPB prep Keck JM, et al. (2011)
    View Run MLP2 #19 Asynchronous Prep1-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #25 Asynchronous Prep3-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2 Keck JM, et al. (2011)
    View Run MLP2 #21 Asynchronous Prep2-IMAC Phosphopeptide enrichment A1 Keck JM, et al. (2011)
    View Run MLP2 #22 Asynchronous Prep2-IMAC Phosphopeptide enrichment B1-2 Keck JM, et al. (2011)
    View Run MLP2 #34 Asynchronous SPB prep Keck JM, et al. (2011)
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #35 Asynchronous SPB prep Keck JM, et al. (2011)
    View Run MLP2 #31 Asynchronous Prep (Protease cleavage) Keck JM, et al. (2011)
    View Run MLP2 #04 Alpha Factor Prep2 Keck JM, et al. (2011)
    View Run MLP2 #03 Alpha Factor Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #29 Asynchronous Prep5-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #14 Mitotic Prep2-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #13 Mitotic Prep2-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #10 Mitotic Prep1-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #02 Alpha Factor Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)
    View Run MLP2 #30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #11 Mitotic Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)
    View Run MLP2 #01 Alpha factor Prep1-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #28 Asynchronous Prep5-TiO2 Phosphopeptide Enrichment Keck JM, et al. (2011)
    View Run MLP2 #06 Alpha Factor Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #16 Mitotic-New search criteria Keck JM, et al. (2011)
    View Run MLP2 #15 Mitotic 2nd-TiO2 enriched, new search criteria Keck JM, et al. (2011)
    View Run MLP2 #19b Asynchronous Prep1-new search criteria Keck JM, et al. (2011)
    View Run MLP2 #05 Alpha Factor Prep2-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #07 Alpha Factor Prep3-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #09 Alpha Factor Prep4-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run None Entered #17 Mitotic Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run None Entered #18 Mitotic Prep4-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #19a Asynchronous Prep1-new search criteria Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data NBP1 and SPC110 Sundin BA, et al. (2004)
    View Data NBP1 and SPC110 Sundin BA, et al. (2004)
    View Data SPC110 and SPC42 Huh WK, et al. (2003)
    View Data SPC110 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..77] deduced N/A No confident structure predictions are available.
    2 View Details [78..409] PSI-BLAST 63.68867 Heavy meromyosin subfragment
    3 View Details [410..478] PSI-BLAST 24.39794 Tropomyosin
    4 View Details [479..649] ORFEUS 14.1 alpha-actinin
    5 View Details [650..847] ORFEUS 14.1 alpha-actinin
    6 View Details [848..944] deduced N/A Confident ab initio structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2 No functions predicted.
    3 No functions predicted.
    4
    Term Confidence Notes
  • binding
    		•  2.87991790206998 bayes_pls_golite062009
  • cytoskeletal protein binding
    		•  2.1457670954804 bayes_pls_golite062009
  • transcription regulator activity
    		•  2.122580758085 bayes_pls_golite062009
  • DNA binding
    		•  1.98453325263719 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.93681952439958 bayes_pls_golite062009
  • actin binding
    		•  1.48871115327078 bayes_pls_golite062009
  • transcription factor activity
    		•  1.36872096268289 bayes_pls_golite062009
  • RNA polymerase II transcription factor activity
    		•  1.09150919349912 bayes_pls_golite062009
  • sequence-specific DNA binding
    		•  1.03881604759993 bayes_pls_golite062009
  • protein binding
    		•  1.03780839342631 bayes_pls_golite062009
  • chromatin binding
    		•  0.545627011042409 bayes_pls_golite062009
  • actin filament binding
    		•  0.26311995282891 bayes_pls_golite062009
  • specific RNA polymerase II transcription factor activity
    		•  0.18367441245103 bayes_pls_golite062009
  • transcription activator activity
    		•  0.174325083775401 bayes_pls_golite062009
  • transcription factor binding
    		•  0.14097776282047 bayes_pls_golite062009
  • centromeric DNA binding
    		•  0.09491254824772 bayes_pls_golite062009
    5 No functions predicted.
    6 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.86

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle